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The map label for this gene is qor [C]

Identifier: 116872003

GI number: 116872003

Start: 621849

End: 622790

Strand: Direct

Name: qor [C]

Synonym: lwe0583

Alternate gene names: 116872003

Gene position: 621849-622790 (Clockwise)

Preceding gene: 116872002

Following gene: 116872004

Centisome position: 22.1

GC content: 38.96

Gene sequence:

>942_bases
ATGAAAGCAGTTGTAATTGAAAATTATGGTGGAAAAGAAGAATTAAAAGAAAAAGAAGTTGCAATGCCAAAAGCTGGTAA
AAACCAAGTAATTGTAAAAGAGGCAGCAACATCAATTAATCCGATTGATTGGAAACTTCGTGAAGGATACCTAAAACAAA
TGATGGACTGGGAATTCCCAATTATTTTAGGTTGGGATGTAGCGGGCGTTATTTCAGAAGTTGGAGAAGGCGTAACAGAT
TGGAAAGTTGGGGATGAAGTATTTGCACGTCCTGAAACGACTCGTTTTGGTACGTATGCAGAATATACAGCAGTAGATGA
CCACTTGCTTGCACCACTTCCAGAAGGCATTAGTTTTGATGAAGCAGCATCTATTCCTCTTGCAGGACTAACTGCATGGC
AAGCACTATTTGATCATGCAAATCTACAAAAAGGTGAAAAAGTCTTAATTCATGCTGGTGCTGGCGGTGTAGGAACACTG
GCGATTCAACTTGCCAAACATGCTGGAGCAGAGGTAATCACAACAGCTAGCGCGAAGAATCATGAATTACTCAAATCGCT
TGGTGCAGATCAAGTTATTGATTATAAAGAAGTTAATTTTAAAGATGTACTTTCTGATATTGATGTTGTTTTTGATACAA
TGGGCGGCGAAATCGAAACTGATAGCTATGATGTATTAAAAGCTGGAACTGGTCGTTTAGTTAGTATCGTTGGGATTTCA
AATGAAGATCGTGCAAAAGAGAAAAACGTTACAGCGACAGGAATATGGCTTCAACCAAACGGCGAGCAGCTGAAAGAACT
AGGGAAATTACTTGCTGATAAAACAGTTAAACCAATTGTTGGCGCAACTTTCCCGTTCTCTGAAAAAGGTGTTTTCGATG
CACATGCGTTAAGTGAAACGCACCATGCGGTTGGAAAAATAGTCATTTCATTCAATAAATAA

Upstream 100 bases:

>100_bases
CAGAAGCTTTAGAGGCATTTTCAGAGGATGAAGTTCGTGCGTTACTAAAATCAGTCACAAAAATGATGAATAATGTAGAG
AAAATGGAGGAGAAGTGAAA

Downstream 100 bases:

>100_bases
GTTTTTCGGAAAGCTAGATGCTATCATCTAGCTTTTTTTGTTGTTTGCTGGAATAGATTGGATAGGCTATAATAGCAAAA
AAAGGTGGTGTCTGAATGGA

Product: alcohol dehydrogenase, zinc-dependent

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFPIILGWDVAGVISEVGEGVTD
WKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFDEAASIPLAGLTAWQALFDHANLQKGEKVLIHAGAGGVGTL
AIQLAKHAGAEVITTASAKNHELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGEIETDSYDVLKAGTGRLVSIVGIS
NEDRAKEKNVTATGIWLQPNGEQLKELGKLLADKTVKPIVGATFPFSEKGVFDAHALSETHHAVGKIVISFNK

Sequences:

>Translated_313_residues
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFPIILGWDVAGVISEVGEGVTD
WKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFDEAASIPLAGLTAWQALFDHANLQKGEKVLIHAGAGGVGTL
AIQLAKHAGAEVITTASAKNHELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGEIETDSYDVLKAGTGRLVSIVGIS
NEDRAKEKNVTATGIWLQPNGEQLKELGKLLADKTVKPIVGATFPFSEKGVFDAHALSETHHAVGKIVISFNK
>Mature_313_residues
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFPIILGWDVAGVISEVGEGVTD
WKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFDEAASIPLAGLTAWQALFDHANLQKGEKVLIHAGAGGVGTL
AIQLAKHAGAEVITTASAKNHELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGEIETDSYDVLKAGTGRLVSIVGIS
NEDRAKEKNVTATGIWLQPNGEQLKELGKLLADKTVKPIVGATFPFSEKGVFDAHALSETHHAVGKIVISFNK

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=354, Percent_Identity=33.8983050847458, Blast_Score=154, Evalue=8e-38,
Organism=Homo sapiens, GI194239674, Length=313, Percent_Identity=30.6709265175719, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI13236495, Length=313, Percent_Identity=30.6709265175719, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI22538446, Length=350, Percent_Identity=30.2857142857143, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI22538444, Length=350, Percent_Identity=30.2857142857143, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI194239676, Length=301, Percent_Identity=32.890365448505, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI18379349, Length=260, Percent_Identity=33.8461538461538, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI24308257, Length=235, Percent_Identity=29.3617021276596, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI28557745, Length=232, Percent_Identity=31.0344827586207, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI90669511, Length=183, Percent_Identity=33.879781420765, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI67078404, Length=282, Percent_Identity=28.3687943262411, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI67078406, Length=260, Percent_Identity=27.6923076923077, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1790485, Length=227, Percent_Identity=34.3612334801762, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790045, Length=335, Percent_Identity=24.1791044776119, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=307, Percent_Identity=30.6188925081433, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI71987554, Length=349, Percent_Identity=29.512893982808, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17562876, Length=309, Percent_Identity=28.4789644012945, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17536829, Length=270, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17556000, Length=254, Percent_Identity=28.3464566929134, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71988145, Length=222, Percent_Identity=24.3243243243243, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=336, Percent_Identity=27.6785714285714, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324195, Length=303, Percent_Identity=29.042904290429, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=245, Percent_Identity=29.7959183673469, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=290, Percent_Identity=28.2758620689655, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=226, Percent_Identity=29.2035398230088, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319945, Length=256, Percent_Identity=30.078125, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323493, Length=256, Percent_Identity=28.90625, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319401, Length=159, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581339, Length=361, Percent_Identity=28.5318559556787, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581341, Length=361, Percent_Identity=27.9778393351801, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45550423, Length=241, Percent_Identity=26.5560165975104, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 33856; Mature: 33856

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFP
CCEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCC
IILGWDVAGVISEVGEGVTDWKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFD
EEECCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCC
EAASIPLAGLTAWQALFDHANLQKGEKVLIHAGAGGVGTLAIQLAKHAGAEVITTASAKN
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCC
HELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGEIETDSYDVLKAGTGRLVSIVGIS
HHHHHHCCCCCEECHHCCCHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCEEEEEECC
NEDRAKEKNVTATGIWLQPNGEQLKELGKLLADKTVKPIVGATFPFSEKGVFDAHALSET
CCCHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHH
HHAVGKIVISFNK
HHCEEEEEEEECC
>Mature Secondary Structure
MKAVVIENYGGKEELKEKEVAMPKAGKNQVIVKEAATSINPIDWKLREGYLKQMMDWEFP
CCEEEEECCCCHHHHHHHHHCCCCCCCCCEEEEHHHCCCCCCCHHHHHHHHHHHHCCCCC
IILGWDVAGVISEVGEGVTDWKVGDEVFARPETTRFGTYAEYTAVDDHLLAPLPEGISFD
EEECCCHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCHHHCCCCCCCCCC
EAASIPLAGLTAWQALFDHANLQKGEKVLIHAGAGGVGTLAIQLAKHAGAEVITTASAKN
CCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCCC
HELLKSLGADQVIDYKEVNFKDVLSDIDVVFDTMGGEIETDSYDVLKAGTGRLVSIVGIS
HHHHHHCCCCCEECHHCCCHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCCEEEEEECC
NEDRAKEKNVTATGIWLQPNGEQLKELGKLLADKTVKPIVGATFPFSEKGVFDAHALSET
CCCHHHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHH
HHAVGKIVISFNK
HHCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]