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The map label for this gene is ytwF [H]

Identifier: 116871999

GI number: 116871999

Start: 618225

End: 618524

Strand: Direct

Name: ytwF [H]

Synonym: lwe0579

Alternate gene names: 116871999

Gene position: 618225-618524 (Clockwise)

Preceding gene: 116871998

Following gene: 116872002

Centisome position: 21.97

GC content: 36.33

Gene sequence:

>300_bases
ATGTATCAATCCATTACTGCAAATGATTTAGAACAAGAATTGAAAGAATCCACGCAAAATATACTAGATGTAAGAGACGC
AGATGCCTTTATTGAAGGTCACATTCCAAATGCCATAAATATTCCGATTAATGAATTACCAGAAAAGCTTGCAACGCTTG
ATAAAAAGAAAGCTTATACAATTATTTGTTATGCAGGTGGACGTTCTGAGCGAGCAAGTCAATTTTTAGCTGCGGAAGGT
TTTGATGTAACGAATGTAATGGGTGGCATGGGTGCTTTTCGTGGTACTACCACTAAATAA

Upstream 100 bases:

>100_bases
TGGTGTAAAAAGCCACATTGTCTTTTTTGAGCATAAAATTTGTTTGCCTAGCAAGAAGGCGGTATGGTAAATAAAGAAGA
ATTTGAAGAGGTGAAAATAG

Downstream 100 bases:

>100_bases
ACAAAGAAGTCGTGACTTGAATATTAAGTCACGACTTCTTTTTAGTGTTGTTTTCTATTGCGGAAAAAGATAATTGCTAA
TCCCGCTAAAGCGATACCGA

Product: rhodanese-like domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 99; Mature: 99

Protein sequence:

>99_residues
MYQSITANDLEQELKESTQNILDVRDADAFIEGHIPNAINIPINELPEKLATLDKKKAYTIICYAGGRSERASQFLAAEG
FDVTNVMGGMGAFRGTTTK

Sequences:

>Translated_99_residues
MYQSITANDLEQELKESTQNILDVRDADAFIEGHIPNAINIPINELPEKLATLDKKKAYTIICYAGGRSERASQFLAAEG
FDVTNVMGGMGAFRGTTTK
>Mature_99_residues
MYQSITANDLEQELKESTQNILDVRDADAFIEGHIPNAINIPINELPEKLATLDKKKAYTIICYAGGRSERASQFLAAEG
FDVTNVMGGMGAFRGTTTK

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 10839; Mature: 10839

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSITANDLEQELKESTQNILDVRDADAFIEGHIPNAINIPINELPEKLATLDKKKAYT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEECCHHHHHHHHHHCCCCCCEE
IICYAGGRSERASQFLAAEGFDVTNVMGGMGAFRGTTTK
EEEEECCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MYQSITANDLEQELKESTQNILDVRDADAFIEGHIPNAINIPINELPEKLATLDKKKAYT
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEECCHHHHHHHHHHCCCCCCEE
IICYAGGRSERASQFLAAEGFDVTNVMGGMGAFRGTTTK
EEEEECCCCHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]