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The map label for this gene is prs [H]

Identifier: 116871886

GI number: 116871886

Start: 499617

End: 500552

Strand: Direct

Name: prs [H]

Synonym: lwe0466

Alternate gene names: 116871886

Gene position: 499617-500552 (Clockwise)

Preceding gene: 116871885

Following gene: 116871887

Centisome position: 17.75

GC content: 40.38

Gene sequence:

>936_bases
ATGGCAGGCAAAATGAAACTTTTTTCTGTGACGAGTGAGAGACCACTTGCGACAAAAATTGCAGATTATTTAGATATACC
TTTATGTGAGGTGGAGCTTCAGAAGTTCAGTGACGGAGAAGTAAAAGTTAATATTGAGGAGAGTATCCGCGGGACGAATG
CGTATGTAGTGCAATCCATGAACGCAAACGTCAATGAACGCTTAATGGAACTTTTAATTATGGTAGATGCTTTAAAACGT
GCTTCCGTTCACTCAATCAATATAATTATGCCTTATTATGGCTATGCTCGTCAGGACAGAAAAGCACGCTCAAGAGAACC
TATAACTGCCAAATTAATGGCAAATCTTATCCAACGTGCAGGTGCTGATCGTTTAATCACAGTTGATTTGCATGCAGCTC
AGATTCAGGGCTTTTTCAATATTCCAATCGATCATCTTTCAGCAGTTCCGCTTATTGGTGATTATTTGATAGAAAAATAT
GGAAATGAGGATGTTGTAGTAGTTGCGCCTGACCATAGTGGTGTAGTTCGTGCGCGTAGAATTGCTGACAGACTAAATGC
TCCTATAGCCATTTTAAACCGTAAACCACGACCACACGAAGATGAAATTATGAGTGTGATTGGCGATGTCAAAGGTAAAG
TTGCGATAGTTGTCGATGATATTATCGATACCGGAGTTCGCGCTACGACCTCAGCTGATATTTTACTCGAAAAAGGTGCG
CTAGAAGTTATAGCCTGCGCAACCCATTCAGTTATGGCTGGAAACGCTACTGAACGCTTGCAAAACTCAAGAATCAAAGA
AGTCATCACATCCGACTCCATTGATTTACCAGAAGAAAAACAATTCGATAAATTAACTACTATCTCCATCGGTCGAATTC
TAGGTCGCGCAATTGAAGGCGTACAAGAAAACCGTTCATTACATCCGTTATTTTAA

Upstream 100 bases:

>100_bases
GCCTTTTTTGTTTTGTGAAACATTTTAGCTAGTAGACAACACCTAGACAGTTCTATATAATAATAAGGAGAAGAAATTAG
TTCGGCGGAAGGAGATAGCA

Downstream 100 bases:

>100_bases
GGACAAACTATAATTAATTCAAAACAGCAACTGAGTAAAGTTGCTGTTTTTTTGTGTAAAATAGCAAATTCTAACCAAAA
ATCTGGTGTTTTATGCTAAA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2 [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKVNIEESIRGTNAYVVQSMNANVNERLMELLIMVDALKR
ASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRAGADRLITVDLHAAQIQGFFNIPIDHLSAVPLIGDYLIEKY
GNEDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
LEVIACATHSVMAGNATERLQNSRIKEVITSDSIDLPEEKQFDKLTTISIGRILGRAIEGVQENRSLHPLF

Sequences:

>Translated_311_residues
MAGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKVNIEESIRGTNAYVVQSMNANVNERLMELLIMVDALKR
ASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRAGADRLITVDLHAAQIQGFFNIPIDHLSAVPLIGDYLIEKY
GNEDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
LEVIACATHSVMAGNATERLQNSRIKEVITSDSIDLPEEKQFDKLTTISIGRILGRAIEGVQENRSLHPLF
>Mature_310_residues
AGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKVNIEESIRGTNAYVVQSMNANVNERLMELLIMVDALKRA
SVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRAGADRLITVDLHAAQIQGFFNIPIDHLSAVPLIGDYLIEKYG
NEDVVVVAPDHSGVVRARRIADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGAL
EVIACATHSVMAGNATERLQNSRIKEVITSDSIDLPEEKQFDKLTTISIGRILGRAIEGVQENRSLHPLF

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=42.3076923076923, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=41.6129032258065, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI28557709, Length=310, Percent_Identity=41.2903225806452, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=41.2140575079872, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI4506133, Length=346, Percent_Identity=33.8150289017341, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI194018537, Length=329, Percent_Identity=32.8267477203647, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI310128524, Length=142, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310115209, Length=142, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310118259, Length=142, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI310119946, Length=142, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=43.7299035369775, Blast_Score=255, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI25149168, Length=310, Percent_Identity=42.9032258064516, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17554702, Length=310, Percent_Identity=42.9032258064516, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI71989924, Length=310, Percent_Identity=42.9032258064516, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=42.8571428571429, Blast_Score=241, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17570245, Length=341, Percent_Identity=32.5513196480938, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321776, Length=310, Percent_Identity=39.6774193548387, Blast_Score=225, Evalue=7e-60,
Organism=Saccharomyces cerevisiae, GI6320946, Length=310, Percent_Identity=38.0645161290323, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=39.0476190476191, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6322667, Length=201, Percent_Identity=36.318407960199, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324511, Length=92, Percent_Identity=42.3913043478261, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=312, Percent_Identity=40.7051282051282, Blast_Score=230, Evalue=7e-61,
Organism=Drosophila melanogaster, GI45551540, Length=335, Percent_Identity=38.2089552238806, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24651458, Length=342, Percent_Identity=29.5321637426901, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24651456, Length=342, Percent_Identity=29.5321637426901, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI281362873, Length=342, Percent_Identity=29.5321637426901, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24651454, Length=342, Percent_Identity=29.5321637426901, Blast_Score=148, Evalue=5e-36,
Organism=Drosophila melanogaster, GI45552010, Length=179, Percent_Identity=35.195530726257, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24651462, Length=179, Percent_Identity=35.195530726257, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24651464, Length=179, Percent_Identity=35.195530726257, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34372; Mature: 34241

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKVNIEESIRGTNAYVVQSM
CCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEHHHCCCCCEEEEEEC
NANVNERLMELLIMVDALKRASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
GADRLITVDLHAAQIQGFFNIPIDHLSAVPLIGDYLIEKYGNEDVVVVAPDHSGVVRARR
CCCCEEEEEEEHHHHCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
IADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
HHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCHHEEEECCC
LEVIACATHSVMAGNATERLQNSRIKEVITSDSIDLPEEKQFDKLTTISIGRILGRAIEG
EEEEEEHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VQENRSLHPLF
HHHCCCCCCCC
>Mature Secondary Structure 
AGKMKLFSVTSERPLATKIADYLDIPLCEVELQKFSDGEVKVNIEESIRGTNAYVVQSM
CCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEHHHCCCCCEEEEEEC
NANVNERLMELLIMVDALKRASVHSINIIMPYYGYARQDRKARSREPITAKLMANLIQRA
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHC
GADRLITVDLHAAQIQGFFNIPIDHLSAVPLIGDYLIEKYGNEDVVVVAPDHSGVVRARR
CCCCEEEEEEEHHHHCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
IADRLNAPIAILNRKPRPHEDEIMSVIGDVKGKVAIVVDDIIDTGVRATTSADILLEKGA
HHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCCCHHEEEECCC
LEVIACATHSVMAGNATERLQNSRIKEVITSDSIDLPEEKQFDKLTTISIGRILGRAIEG
EEEEEEHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VQENRSLHPLF
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]