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The map label for this gene is rlmN [H]

Identifier: 116871870

GI number: 116871870

Start: 486165

End: 487268

Strand: Direct

Name: rlmN [H]

Synonym: lwe0450

Alternate gene names: 116871870

Gene position: 486165-487268 (Clockwise)

Preceding gene: 116871869

Following gene: 116871871

Centisome position: 17.28

GC content: 38.5

Gene sequence:

>1104_bases
ATGGAAAAGAGCTCCATTTATGGATTAACCTGGACAAAATTAACAGAATGGCTAGAAGCACACGGCCAAAAGAAATTCCG
CGCGACACAAGTGTGGGACTGGCTTTATAGAAAACGTGTTAAAACTTTTGAAGAAATGAGTAACGTACCAAAAGAAACAA
TTGAACTATTGACAGCGAATTTTGTGATGAACACTTTAGAGGAACAAGTGGTACAAGAATCTACAGATGGCACGACGAAA
TATTTATTCAAGCTGAGTGACGGGAATTTAATTGAAACCGTGATGATGAAGCAAGAATATGGCTTGTCTGTTTGTGTGAC
GACCCAAGTTGGCTGTAATATTGGCTGTACTTTTTGCGCAAGTGGGCTTTTGAAAAAAAGTCGTGATTTAACTGCTGGTG
AGATTGTGGAACAAATTATGAATGTGCAGCATTATTTGGATGGACGTAATTTGGAAGAACGTGTAAGTCATGTGGTTGTA
ATGGGAATCGGCGAACCGTTTGATAATTATGATAATGTGATGGATTTCTTACGCGTGATTAATCATGATAAAGGTTTGGC
AATCGGTGCACGTCATATCACTGTTTCTACAAGTGGACTCGCACCGCGTATTATTGATTTCGCGAATGAGGATTTCCAAG
TGAACCTAGCAATTTCCTTACATGCGCCAAACAATGAACTACGGACGAGCATTATGCGTATTAATAAAACCTATTCTATC
GAGAAATTGATGGAGGCGATTCATTATTACGTAAATAAAACCAACCGCCGAATTACTTTTGAATATATTATGTTAAAAGG
TGTAAACGACCATAAAAAAGAAGCGCTCGAACTTGCTGCACTTCTGGGCGAACATCGTCATTTAGCTTATGTTAACTTGA
TTCCTTATAACCCAGTGGATGAACATATTGATTACGAACGTAGTACAAAAGAAGATGTACTTGCATTTTATGACACGCTT
AAGAAAAATGGTATAAATTGCGTTATTCGCCGTGAACATGGAACAGATATTGATGCTGCATGTGGGCAACTACGTAGTAA
ACAAATCAAACGAGTTGGCGTGCGTGAACGAATGAAACAAAAGCAAGCAGCACTAGAAGAATAA

Upstream 100 bases:

>100_bases
CAGGACAAACGACTGATATTTACGAAATAGCATTGCTTGTGAACCATAAAACAGCTATAATCTTTATAGACGAAAACAAG
ACCTGAAAAGAGGCTGAACA

Downstream 100 bases:

>100_bases
CATTCAAACTATCTGGTTTCCATTTGGAGATTGGATAGTTTTTGTTTATACTTAAGTTATCTAAAGCAATTGAAGGAGGA
CAAAAATCATGAAAGTAGAG

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTANFVMNTLEEQVVQESTDGTTK
YLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCASGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVV
MGIGEPFDNYDNVMDFLRVINHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVDEHIDYERSTKEDVLAFYDTL
KKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQKQAALEE

Sequences:

>Translated_367_residues
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTANFVMNTLEEQVVQESTDGTTK
YLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCASGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVV
MGIGEPFDNYDNVMDFLRVINHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVDEHIDYERSTKEDVLAFYDTL
KKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQKQAALEE
>Mature_367_residues
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTANFVMNTLEEQVVQESTDGTTK
YLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCASGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVV
MGIGEPFDNYDNVMDFLRVINHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVDEHIDYERSTKEDVLAFYDTL
KKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQKQAALEE

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=364, Percent_Identity=34.8901098901099, Blast_Score=214, Evalue=8e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 42144; Mature: 42144

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTAN
CCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FVMNTLEEQVVQESTDGTTKYLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCA
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
SGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVVMGIGEPFDNYDNVMDFLRVI
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCCHHHHHHHHHHH
NHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
CCCCCCEEEEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCH
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVD
HHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
EHIDYERSTKEDVLAFYDTLKKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQ
HHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQAALEE
HHHHCCC
>Mature Secondary Structure
MEKSSIYGLTWTKLTEWLEAHGQKKFRATQVWDWLYRKRVKTFEEMSNVPKETIELLTAN
CCCCCEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FVMNTLEEQVVQESTDGTTKYLFKLSDGNLIETVMMKQEYGLSVCVTTQVGCNIGCTFCA
HHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
SGLLKKSRDLTAGEIVEQIMNVQHYLDGRNLEERVSHVVVMGIGEPFDNYDNVMDFLRVI
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCCHHHHHHHHHHH
NHDKGLAIGARHITVSTSGLAPRIIDFANEDFQVNLAISLHAPNNELRTSIMRINKTYSI
CCCCCCEEEEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCCH
EKLMEAIHYYVNKTNRRITFEYIMLKGVNDHKKEALELAALLGEHRHLAYVNLIPYNPVD
HHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCH
EHIDYERSTKEDVLAFYDTLKKNGINCVIRREHGTDIDAACGQLRSKQIKRVGVRERMKQ
HHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KQAALEE
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]