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The map label for this gene is fruA [H]

Identifier: 116871806

GI number: 116871806

Start: 398436

End: 398900

Strand: Direct

Name: fruA [H]

Synonym: lwe0386

Alternate gene names: 116871806

Gene position: 398436-398900 (Clockwise)

Preceding gene: 116871805

Following gene: 116871807

Centisome position: 14.16

GC content: 35.91

Gene sequence:

>465_bases
ATGGAAGTAAAAGATATTTTAACAAAAGAATTAGTTGTTTTTGATTTAGAAGCAACGACCAAAGAAGCAGCAGTGGAAGA
AATGGCGGAAATACTGAATAAACATGGCATTTTAGCAGATAAAGCGACATATATTCAAGCCGTTTTGGAACGAGAAGCGC
ATTCAACAACTGGAATAGGTAATAACATTGCTATCCCACACGGAAAAAGTGAGAGTGTGACAAAATCAGCCATTGTTTTT
GCAAGAACGAAAGAAATGATTGAGTGGGAATCACTGGATGATGAACCGGTAAACATGATTTTTTTACTGGCAATTACTGA
TTCCGACAAAACAAACGGACATTTGAAAATCCTTGCTGAAATTGCAACGAAACTAATGGACGATGATATTGTCGAAAGTT
TAAAAAACACAAGAGACGAAGAAGAAGTCATTCGAATTTTAAATGGAGGCGTAGTAGAGATATGA

Upstream 100 bases:

>100_bases
AGTTTTATAAATTTATTGATAATAAAAAATGGCTTGAAAAGCTGACACAACTAAAAAGTGCCGCAGATATTTATGAGCAT
TTGTTAAAGGATGGGATGTA

Downstream 100 bases:

>100_bases
AACGTAAAATTATTGCAGTAACCGCATGTGCGACAGGTGTTGCGCATACTTATATGGCAGCGCAAGCACTTAAAAAAGGC
GCGAAAAAAATGGGTAACCT

Product: PTS system, fructose-specific, IIA component

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MEVKDILTKELVVFDLEATTKEAAVEEMAEILNKHGILADKATYIQAVLEREAHSTTGIGNNIAIPHGKSESVTKSAIVF
ARTKEMIEWESLDDEPVNMIFLLAITDSDKTNGHLKILAEIATKLMDDDIVESLKNTRDEEEVIRILNGGVVEI

Sequences:

>Translated_154_residues
MEVKDILTKELVVFDLEATTKEAAVEEMAEILNKHGILADKATYIQAVLEREAHSTTGIGNNIAIPHGKSESVTKSAIVF
ARTKEMIEWESLDDEPVNMIFLLAITDSDKTNGHLKILAEIATKLMDDDIVESLKNTRDEEEVIRILNGGVVEI
>Mature_154_residues
MEVKDILTKELVVFDLEATTKEAAVEEMAEILNKHGILADKATYIQAVLEREAHSTTGIGNNIAIPHGKSESVTKSAIVF
ARTKEMIEWESLDDEPVNMIFLLAITDSDKTNGHLKILAEIATKLMDDDIVESLKNTRDEEEVIRILNGGVVEI

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=32.4324324324324, Blast_Score=87, Evalue=5e-19,
Organism=Escherichia coli, GI2367327, Length=142, Percent_Identity=27.4647887323944, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI1786951, Length=149, Percent_Identity=25.503355704698, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1790027, Length=146, Percent_Identity=30.8219178082192, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1788726, Length=146, Percent_Identity=28.7671232876712, Blast_Score=67, Evalue=5e-13,
Organism=Escherichia coli, GI1788494, Length=152, Percent_Identity=28.9473684210526, Blast_Score=60, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17061; Mature: 17061

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVKDILTKELVVFDLEATTKEAAVEEMAEILNKHGILADKATYIQAVLEREAHSTTGIG
CCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
NNIAIPHGKSESVTKSAIVFARTKEMIEWESLDDEPVNMIFLLAITDSDKTNGHLKILAE
CCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
IATKLMDDDIVESLKNTRDEEEVIRILNGGVVEI
HHHHHCCHHHHHHHHCCCCHHHHHHHHCCCEECC
>Mature Secondary Structure
MEVKDILTKELVVFDLEATTKEAAVEEMAEILNKHGILADKATYIQAVLEREAHSTTGIG
CCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
NNIAIPHGKSESVTKSAIVFARTKEMIEWESLDDEPVNMIFLLAITDSDKTNGHLKILAE
CCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHHHH
IATKLMDDDIVESLKNTRDEEEVIRILNGGVVEI
HHHHHCCHHHHHHHHCCCCHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]