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The map label for this gene is ybjI [H]

Identifier: 116871803

GI number: 116871803

Start: 394626

End: 395438

Strand: Direct

Name: ybjI [H]

Synonym: lwe0383

Alternate gene names: 116871803

Gene position: 394626-395438 (Clockwise)

Preceding gene: 116871800

Following gene: 116871805

Centisome position: 14.02

GC content: 38.01

Gene sequence:

>813_bases
TTGATTAGAGCTATTTCTGTAGATATGGACGGGACTTTTTTAGATGAGAATGGTGAATATAATCGTGCTAGATTTGAAAG
AATTTATGCAGAATTAGTGAAGCAGCAGATTAAATTTATTGTTGCGAGCGGAAATCAATATTATCAGCTAAAATCTTTTT
TTCCTGGGAAGGATGAGGAACTTTTTTACGTTGCTGAAAATGGAGCAGTTATTTTTCATCAAGGGGAACTTCGGAGTGTG
AATCGTTTTGAGAAGGGATTAGTTCAAAAGATTTTGCGGACGTTGATTCAAGATTATCAGGATTTGCAAGTGATTCTTTG
TGGGGTCAAGAGTGCGTATTTGTTAAAAGCAGCGGATTCGAATTTTAAAACATTTGCGAAAAAGTATTATTTTGAATTAC
AAGAAGTGGATTCGTTCGATGTGCTTCCGGATGATACATTTATTAAGTTTGCGCTGGATGTGGAAGTGACTAAGACTGGG
CAAATTGTGGAAGATTTGAATCAAACGTTTAATGGAGAAATTCGTGCTGTCGCAAGTGGTCACGGAAGTATCGATATTAT
TATTCCGGGTGTGACGAAAGGGAGCGCCATTCAGCAACTATTGAATGAATGGCGAGTGGCTCCAGATGATTTACTCGCTT
TTGGGGATGCGAATAATGATATCGAAATGTTGCAATTAACTCCGAATAGTTACGCAATGCAAGAAAGTAGTCCGGAAGTC
TTTGCTACTGCAAAATATATTGCTCCTTCTAATAAGGAGGCGGGCGTGTTACAAGTTATAGAAGATTATATGGAAAAAAG
CCAGAGACCTTGA

Upstream 100 bases:

>100_bases
TTAGTATAGCATATTTTATAAGCAAACAAGGAGTAGCAATGTAACACGAAAAAAATCTGATATAATAGAATAAAATTAAC
TTTAAAAGGAGCTTAAATAA

Downstream 100 bases:

>100_bases
TATGGTCTTTGGCTTTTTCTATTTCAATAAACTAAGAAATACGACGCCCACCATAATTAGCAGTATGCCAGAAAGGATAA
AGGTCCATTCCAGTGGGCTT

Product: Cof-like hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MIRAISVDMDGTFLDENGEYNRARFERIYAELVKQQIKFIVASGNQYYQLKSFFPGKDEELFYVAENGAVIFHQGELRSV
NRFEKGLVQKILRTLIQDYQDLQVILCGVKSAYLLKAADSNFKTFAKKYYFELQEVDSFDVLPDDTFIKFALDVEVTKTG
QIVEDLNQTFNGEIRAVASGHGSIDIIIPGVTKGSAIQQLLNEWRVAPDDLLAFGDANNDIEMLQLTPNSYAMQESSPEV
FATAKYIAPSNKEAGVLQVIEDYMEKSQRP

Sequences:

>Translated_270_residues
MIRAISVDMDGTFLDENGEYNRARFERIYAELVKQQIKFIVASGNQYYQLKSFFPGKDEELFYVAENGAVIFHQGELRSV
NRFEKGLVQKILRTLIQDYQDLQVILCGVKSAYLLKAADSNFKTFAKKYYFELQEVDSFDVLPDDTFIKFALDVEVTKTG
QIVEDLNQTFNGEIRAVASGHGSIDIIIPGVTKGSAIQQLLNEWRVAPDDLLAFGDANNDIEMLQLTPNSYAMQESSPEV
FATAKYIAPSNKEAGVLQVIEDYMEKSQRP
>Mature_270_residues
MIRAISVDMDGTFLDENGEYNRARFERIYAELVKQQIKFIVASGNQYYQLKSFFPGKDEELFYVAENGAVIFHQGELRSV
NRFEKGLVQKILRTLIQDYQDLQVILCGVKSAYLLKAADSNFKTFAKKYYFELQEVDSFDVLPDDTFIKFALDVEVTKTG
QIVEDLNQTFNGEIRAVASGHGSIDIIIPGVTKGSAIQQLLNEWRVAPDDLLAFGDANNDIEMLQLTPNSYAMQESSPEV
FATAKYIAPSNKEAGVLQVIEDYMEKSQRP

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=269, Percent_Identity=41.635687732342, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787043, Length=266, Percent_Identity=36.4661654135338, Blast_Score=187, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30546; Mature: 30546

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRAISVDMDGTFLDENGEYNRARFERIYAELVKQQIKFIVASGNQYYQLKSFFPGKDEE
CEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCE
LFYVAENGAVIFHQGELRSVNRFEKGLVQKILRTLIQDYQDLQVILCGVKSAYLLKAADS
EEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NFKTFAKKYYFELQEVDSFDVLPDDTFIKFALDVEVTKTGQIVEDLNQTFNGEIRAVASG
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECC
HGSIDIIIPGVTKGSAIQQLLNEWRVAPDDLLAFGDANNDIEMLQLTPNSYAMQESSPEV
CCEEEEEECCCCCCHHHHHHHHHHCCCHHHHEEECCCCCCEEEEEECCCCEECCCCCCCE
FATAKYIAPSNKEAGVLQVIEDYMEKSQRP
EEEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRAISVDMDGTFLDENGEYNRARFERIYAELVKQQIKFIVASGNQYYQLKSFFPGKDEE
CEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCE
LFYVAENGAVIFHQGELRSVNRFEKGLVQKILRTLIQDYQDLQVILCGVKSAYLLKAADS
EEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
NFKTFAKKYYFELQEVDSFDVLPDDTFIKFALDVEVTKTGQIVEDLNQTFNGEIRAVASG
CHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEECC
HGSIDIIIPGVTKGSAIQQLLNEWRVAPDDLLAFGDANNDIEMLQLTPNSYAMQESSPEV
CCEEEEEECCCCCCHHHHHHHHHHCCCHHHHEEECCCCCCEEEEEECCCCEECCCCCCCE
FATAKYIAPSNKEAGVLQVIEDYMEKSQRP
EEEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]