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The map label for this gene is lcmA [H]

Identifier: 116871711

GI number: 116871711

Start: 315124

End: 316179

Strand: Direct

Name: lcmA [H]

Synonym: lwe0291

Alternate gene names: 116871711

Gene position: 315124-316179 (Clockwise)

Preceding gene: 116871710

Following gene: 116871712

Centisome position: 11.2

GC content: 32.95

Gene sequence:

>1056_bases
ATGATAGTCAATGCGGTTGATCTGTTTTGTGGTGTAGGTGGCCTAACTTGTGGAGTTCAGAAAACGGGGATCAATGTTAT
TGCGGGTTATGATATTGCTAAAGAATGTCAGTATTCATATGAGTATAATAATAAGGCAAGATTTATTCATAAAGATATAA
AAGATATCTCCGATAATGAAATATCAGCATTATATCCTGAAAATACGGATATAAGACTTTTAATGGGATGTGCTCCGTGC
CAACCGTTTTCTGCATATAGTCATAGGTATAAAGAAAGTGAAACAAGAAAACAAAAAATGGATTTATTAGATTATTTTGG
AAAACAAGTTGAATTAGTACAGCCGGAAATCGTATCAATGGAAAATGTTCCACAACTTAGAAATGACCCGGTTTTTGAAA
AATTTATTACTTTATTAAAAAGTAATAATTATTTTGTTGATTGGAAAATCGTTTATGCTCCAGAATATGGTGTTCCTCAA
AAAAGAAAAAGACTTTTATTGTTAGCATCTAAAATAGGTGAGATTAGTTTATTAGAGCCAACAAGAACGAAAGATAATTA
TCCAACGGTGAGGGAAGCAATTTCAAGATTGAAGCCAATTAAGGCAGGTGAAACAGATAATGAGGATTTTCTTCATAGGG
CAAGAAAATTATCTGACTTGAATATAAAAAGAATTAAGAGTTCTTTTCCGGGAGGAACTTGGAAAGATTGGGATGAAGAG
CTTTTGCCTAACTGTTATAAACGAAAAAGTGGTGAAAGTTATAAATCAGTATACGGTCGATTGGAATGGGACAAGCCATC
GTCAACTATTACTACTCAATTTGTGGGATATGGGAATGGTAGATTTGGACATCCTGAACAAGATAGGGCTTTGAGTTTGA
GAGAAGGAGCGATTCTACAAACTTTTCCTAAAGATTATCAGTTTGTAGAAAAAATTGAGCAAGCATCTTTTGTTAAACTA
GCTGTTCAAATAGGAAATGCTGTACCTGTTAAATTGGGAGAGGTTATCGGGGAAAGTATAAAAACACATCTAGAAAATAA
TAAATTATTATCATAA

Upstream 100 bases:

>100_bases
AAAACTAAGACGAGAAGAGTGAATATGAGATGGATAATTTTCTACAGTTTTCTTTGTTTTAAAAGTATTATTTAAAAATA
GAAAGATAGGAGGGGCTTCA

Downstream 100 bases:

>100_bases
GGAGAGTGCTAGAGATGAGTGAAAAAGAATACAATCTGGATATTGATCCAAGAATATTAGAATTATTAGGCCCCCATTTG
TATACGAATATCTATTACAT

Product: cytosine methyl transferase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MIVNAVDLFCGVGGLTCGVQKTGINVIAGYDIAKECQYSYEYNNKARFIHKDIKDISDNEISALYPENTDIRLLMGCAPC
QPFSAYSHRYKESETRKQKMDLLDYFGKQVELVQPEIVSMENVPQLRNDPVFEKFITLLKSNNYFVDWKIVYAPEYGVPQ
KRKRLLLLASKIGEISLLEPTRTKDNYPTVREAISRLKPIKAGETDNEDFLHRARKLSDLNIKRIKSSFPGGTWKDWDEE
LLPNCYKRKSGESYKSVYGRLEWDKPSSTITTQFVGYGNGRFGHPEQDRALSLREGAILQTFPKDYQFVEKIEQASFVKL
AVQIGNAVPVKLGEVIGESIKTHLENNKLLS

Sequences:

>Translated_351_residues
MIVNAVDLFCGVGGLTCGVQKTGINVIAGYDIAKECQYSYEYNNKARFIHKDIKDISDNEISALYPENTDIRLLMGCAPC
QPFSAYSHRYKESETRKQKMDLLDYFGKQVELVQPEIVSMENVPQLRNDPVFEKFITLLKSNNYFVDWKIVYAPEYGVPQ
KRKRLLLLASKIGEISLLEPTRTKDNYPTVREAISRLKPIKAGETDNEDFLHRARKLSDLNIKRIKSSFPGGTWKDWDEE
LLPNCYKRKSGESYKSVYGRLEWDKPSSTITTQFVGYGNGRFGHPEQDRALSLREGAILQTFPKDYQFVEKIEQASFVKL
AVQIGNAVPVKLGEVIGESIKTHLENNKLLS
>Mature_351_residues
MIVNAVDLFCGVGGLTCGVQKTGINVIAGYDIAKECQYSYEYNNKARFIHKDIKDISDNEISALYPENTDIRLLMGCAPC
QPFSAYSHRYKESETRKQKMDLLDYFGKQVELVQPEIVSMENVPQLRNDPVFEKFITLLKSNNYFVDWKIVYAPEYGVPQ
KRKRLLLLASKIGEISLLEPTRTKDNYPTVREAISRLKPIKAGETDNEDFLHRARKLSDLNIKRIKSSFPGGTWKDWDEE
LLPNCYKRKSGESYKSVYGRLEWDKPSSTITTQFVGYGNGRFGHPEQDRALSLREGAILQTFPKDYQFVEKIEQASFVKL
AVQIGNAVPVKLGEVIGESIKTHLENNKLLS

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40069; Mature: 40069

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVNAVDLFCGVGGLTCGVQKTGINVIAGYDIAKECQYSYEYNNKARFIHKDIKDISDNE
CEECHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
ISALYPENTDIRLLMGCAPCQPFSAYSHRYKESETRKQKMDLLDYFGKQVELVQPEIVSM
EEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCC
ENVPQLRNDPVFEKFITLLKSNNYFVDWKIVYAPEYGVPQKRKRLLLLASKIGEISLLEP
CCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECC
TRTKDNYPTVREAISRLKPIKAGETDNEDFLHRARKLSDLNIKRIKSSFPGGTWKDWDEE
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHH
LLPNCYKRKSGESYKSVYGRLEWDKPSSTITTQFVGYGNGRFGHPEQDRALSLREGAILQ
HHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEECCCCCHHH
TFPKDYQFVEKIEQASFVKLAVQIGNAVPVKLGEVIGESIKTHLENNKLLS
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIVNAVDLFCGVGGLTCGVQKTGINVIAGYDIAKECQYSYEYNNKARFIHKDIKDISDNE
CEECHHHHHHCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCC
ISALYPENTDIRLLMGCAPCQPFSAYSHRYKESETRKQKMDLLDYFGKQVELVQPEIVSM
EEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHCC
ENVPQLRNDPVFEKFITLLKSNNYFVDWKIVYAPEYGVPQKRKRLLLLASKIGEISLLEP
CCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECC
TRTKDNYPTVREAISRLKPIKAGETDNEDFLHRARKLSDLNIKRIKSSFPGGTWKDWDEE
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHH
LLPNCYKRKSGESYKSVYGRLEWDKPSSTITTQFVGYGNGRFGHPEQDRALSLREGAILQ
HHHHHHHHCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEECCCCCHHH
TFPKDYQFVEKIEQASFVKLAVQIGNAVPVKLGEVIGESIKTHLENNKLLS
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]