Click here to switch to the map view.

The map label for this gene is ltaA [H]

Identifier: 116871704

GI number: 116871704

Start: 309672

End: 310760

Strand: Direct

Name: ltaA [H]

Synonym: lwe0284

Alternate gene names: 116871704

Gene position: 309672-310760 (Clockwise)

Preceding gene: 116871703

Following gene: 116871705

Centisome position: 11.0

GC content: 42.79

Gene sequence:

>1089_bases
ATGACAAACACGCTAAAAACCAGCTACCAAAAAACACCTTATAAACTAGGCGGAAACGGTACGCGGAATATTGGTGTTCT
TACAGAAGCGCTTCAAAATATAGATAATAACCTAGAAAGCGATATATACGGAAGCGGCCCAATTATTGAAGATTTTGAAA
CGAAAATCGCGAAAATTCTTGGTAAACAATCTGCGGTATTTTTTCCGAGTGGGACTATGGCTCAACAAATCGCGTTAAGA
ATTTGGGCTGACCGGAAAGAGAATCAACTCGTGGCATATCATCCACTTTCGCATTTGGAGATTCATGAGCAAGACGGGCT
AAAAGAATTGCAGCAAATCACGCCAATATTACTTGGAACGGCGAATCGACTTGTGACGATTGATGATATTAAAGATCTTC
GCGAGCCAGTTGCTAGTGTGCTTATTGAACTGCCGCAACGAGAAATTGGTGGGCAACTTCCTTCTTTTGAAGAGCTTGAG
GAGATTTCGGAGTATTGTCATGAACAGGGTATTTCATTGCATCTGGACGGAGCGAGGTTATGGGAAATTACACCTTTTTA
TCAGAAGTCTGCGGAAGAAATTTGCGCTTTATTTGATAGTGTATATGTGTCGTTTTATAAAGGAATTGGCGGGATTGCTG
GGGCGATTTTGGCTGGGTCTGATGATTTTGTGCAAGAGGCAAAAATCTGGAAACGTCGATATGGTGGTGATTTGATTAGC
CTGTACCCGTATATTTTGTCCGCGGATTATTATTTTGAAAAACGGATTGGGAAGATGGCGGAGTATTTTGAGGCGGCAAA
AGGATTGGCTGAGCGATTTAATTCGTGTTCGGGCGTGAAGACTGTGCCGGAAGTACCGGTTTCTAATATGTTTCATGTTT
ACTTTGAGAAGTCGGCTGATGAGGTTGGGACGATTTTGACGAAGATTCAGGATGAAACTGGTGTGGGGATTTCGGGTTAT
TTGCAAGAGAAATCGGCGGATGTTTGTGCGTTTGAGGTTTCGATTGGGGATGCGTATGCGGAGATACCTGTGGAGGTTTT
GGATGTTGTGTTTAAACAATTAGAGGTAGGCTCCCCATGCCAAAACTAA

Upstream 100 bases:

>100_bases
CCATCTGCACATATTCCGTATGGTGTTACGAATCAGAAAAAGGATGAGGTATAGGGGTTAGATAACACAAAAAATTTGAT
CGTAAAAGGAGTGAATCTAA

Downstream 100 bases:

>100_bases
TCATCGACGCAGACGATTTCGGTCTATCCAAAGCCATCAACCACGGCATCATCGAAAGCTACAAAACCGGCATTACCACA
TCCACCTTACTCATGCCGAA

Product: threonine aldolase family protein

Products: Acetaldehyde; Glycine [C]

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTNTLKTSYQKTPYKLGGNGTRNIGVLTEALQNIDNNLESDIYGSGPIIEDFETKIAKILGKQSAVFFPSGTMAQQIALR
IWADRKENQLVAYHPLSHLEIHEQDGLKELQQITPILLGTANRLVTIDDIKDLREPVASVLIELPQREIGGQLPSFEELE
EISEYCHEQGISLHLDGARLWEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGSDDFVQEAKIWKRRYGGDLIS
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSADEVGTILTKIQDETGVGISGY
LQEKSADVCAFEVSIGDAYAEIPVEVLDVVFKQLEVGSPCQN

Sequences:

>Translated_362_residues
MTNTLKTSYQKTPYKLGGNGTRNIGVLTEALQNIDNNLESDIYGSGPIIEDFETKIAKILGKQSAVFFPSGTMAQQIALR
IWADRKENQLVAYHPLSHLEIHEQDGLKELQQITPILLGTANRLVTIDDIKDLREPVASVLIELPQREIGGQLPSFEELE
EISEYCHEQGISLHLDGARLWEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGSDDFVQEAKIWKRRYGGDLIS
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSADEVGTILTKIQDETGVGISGY
LQEKSADVCAFEVSIGDAYAEIPVEVLDVVFKQLEVGSPCQN
>Mature_361_residues
TNTLKTSYQKTPYKLGGNGTRNIGVLTEALQNIDNNLESDIYGSGPIIEDFETKIAKILGKQSAVFFPSGTMAQQIALRI
WADRKENQLVAYHPLSHLEIHEQDGLKELQQITPILLGTANRLVTIDDIKDLREPVASVLIELPQREIGGQLPSFEELEE
ISEYCHEQGISLHLDGARLWEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGSDDFVQEAKIWKRRYGGDLISL
YPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSADEVGTILTKIQDETGVGISGYL
QEKSADVCAFEVSIGDAYAEIPVEVLDVVFKQLEVGSPCQN

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=239, Percent_Identity=26.7782426778243, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193206520, Length=204, Percent_Identity=25.9803921568627, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI193206522, Length=204, Percent_Identity=25.9803921568627, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI193206524, Length=204, Percent_Identity=25.9803921568627, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320789, Length=243, Percent_Identity=27.9835390946502, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21355439, Length=213, Percent_Identity=29.1079812206573, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5 [C]

Molecular weight: Translated: 40232; Mature: 40101

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTLKTSYQKTPYKLGGNGTRNIGVLTEALQNIDNNLESDIYGSGPIIEDFETKIAKIL
CCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
GKQSAVFFPSGTMAQQIALRIWADRKENQLVAYHPLSHLEIHEQDGLKELQQITPILLGT
CCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHCC
ANRLVTIDDIKDLREPVASVLIELPQREIGGQLPSFEELEEISEYCHEQGISLHLDGARL
CCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCEE
WEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGSDDFVQEAKIWKRRYGGDLIS
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSAD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHCCHH
EVGTILTKIQDETGVGISGYLQEKSADVCAFEVSIGDAYAEIPVEVLDVVFKQLEVGSPC
HHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEECCHHHHCCHHHHHHHHHHHHCCCCC
QN
CC
>Mature Secondary Structure 
TNTLKTSYQKTPYKLGGNGTRNIGVLTEALQNIDNNLESDIYGSGPIIEDFETKIAKIL
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
GKQSAVFFPSGTMAQQIALRIWADRKENQLVAYHPLSHLEIHEQDGLKELQQITPILLGT
CCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHCC
ANRLVTIDDIKDLREPVASVLIELPQREIGGQLPSFEELEEISEYCHEQGISLHLDGARL
CCCEEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCEE
WEITPFYQKSAEEICALFDSVYVSFYKGIGGIAGAILAGSDDFVQEAKIWKRRYGGDLIS
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
LYPYILSADYYFEKRIGKMAEYFEAAKGLAERFNSCSGVKTVPEVPVSNMFHVYFEKSAD
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHCCHH
EVGTILTKIQDETGVGISGYLQEKSADVCAFEVSIGDAYAEIPVEVLDVVFKQLEVGSPC
HHHHHHHHHHHCCCCCHHHHHHCCCCCEEEEEEEECCHHHHCCHHHHHHHHHHHHCCCCC
QN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.12 {DL-threo-phenylserine}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} [C]

Substrates: L-Threonine [C]

Specific reaction: L-Threonine <==> Acetaldehyde + Glycine [C]

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]