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The map label for this gene is exp5 [H]

Identifier: 116871699

GI number: 116871699

Start: 304182

End: 304643

Strand: Direct

Name: exp5 [H]

Synonym: lwe0279

Alternate gene names: 116871699

Gene position: 304182-304643 (Clockwise)

Preceding gene: 116871698

Following gene: 116871700

Centisome position: 10.81

GC content: 29.0

Gene sequence:

>462_bases
TTGAATTTTTTTAAGAAAAAGACAATTATTTATTCTCCCGGAAATGGATTAATAAAATCTATTGATAAAGTACATGATGA
ACTTTTTTCAACTAAAGCTTTAGGTGATGGTTTTGTATTAGAGCCAAATGAAAGTAGTATTTTTGCTCCAGTTGAAGGAA
CCATTACTTCCATTTTCCCTACAAAACATGCTATTTCTATTAAAACAGCGAGTAATTTAGAGGTTTTAATCCATATTGGG
ATTGATACAGTGGAATTGGATGGGAAGGGCTTTAATCTAAAAGTAAAAGAAGGAACAAAAGTCACCAAAGAAACAGAAAT
TGTAGAAGTTGATTTTCCTTTTTTAGAATCAAATGGAAAAGATACGGATGTTATTGTTATTTTCACAAATTTAAAAGAAA
AACCATTAGCGATTACAGAAGGCCTTATGAAAGTAAATCAAGAAATAGGTACAATTAAATAA

Upstream 100 bases:

>100_bases
GCAATTATTGGGTTACTCATTTCGATGATGGTTTCAGCGATATTAGCATATTCCTTTACTAGTAGGGATGAGAGATTAGC
GTAGGAAAGAAGGAATTAAC

Downstream 100 bases:

>100_bases
TTAATAAAAAATACTCTTAGAAATTCTAGGAGTATTTTTTTGCTAGTTTGACTAAGTTTGAAAATAAATGATAAAATGTA
AATGAATTTAAAATAGGATG

Product: sugar-specific permease EIIA

Products: NA

Alternate protein names: EII-Glc/EIII-Glc; EIICBA-Glc; Glucose permease IIC component; PTS system glucose-specific EIIC component; Glucose-specific phosphotransferase enzyme IIB component; PTS system glucose-specific EIIB component; Glucose-specific phosphotransferase enzyme IIA component; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 153; Mature: 153

Protein sequence:

>153_residues
MNFFKKKTIIYSPGNGLIKSIDKVHDELFSTKALGDGFVLEPNESSIFAPVEGTITSIFPTKHAISIKTASNLEVLIHIG
IDTVELDGKGFNLKVKEGTKVTKETEIVEVDFPFLESNGKDTDVIVIFTNLKEKPLAITEGLMKVNQEIGTIK

Sequences:

>Translated_153_residues
MNFFKKKTIIYSPGNGLIKSIDKVHDELFSTKALGDGFVLEPNESSIFAPVEGTITSIFPTKHAISIKTASNLEVLIHIG
IDTVELDGKGFNLKVKEGTKVTKETEIVEVDFPFLESNGKDTDVIVIFTNLKEKPLAITEGLMKVNQEIGTIK
>Mature_153_residues
MNFFKKKTIIYSPGNGLIKSIDKVHDELFSTKALGDGFVLEPNESSIFAPVEGTITSIFPTKHAISIKTASNLEVLIHIG
IDTVELDGKGFNLKVKEGTKVTKETEIVEVDFPFLESNGKDTDVIVIFTNLKEKPLAITEGLMKVNQEIGTIK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=124, Percent_Identity=37.9032258064516, Blast_Score=101, Evalue=2e-23,
Organism=Escherichia coli, GI1788757, Length=124, Percent_Identity=41.9354838709677, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1790159, Length=121, Percent_Identity=38.8429752066116, Blast_Score=87, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011300 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16810; Mature: 16810

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFFKKKTIIYSPGNGLIKSIDKVHDELFSTKALGDGFVLEPNESSIFAPVEGTITSIFP
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCEEEECC
TKHAISIKTASNLEVLIHIGIDTVELDGKGFNLKVKEGTKVTKETEIVEVDFPFLESNGK
CCEEEEEEECCCEEEEEEECCEEEEECCCCEEEEECCCCCCCCCEEEEEEECCEECCCCC
DTDVIVIFTNLKEKPLAITEGLMKVNQEIGTIK
CCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNFFKKKTIIYSPGNGLIKSIDKVHDELFSTKALGDGFVLEPNESSIFAPVEGTITSIFP
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEECCCCEEEECC
TKHAISIKTASNLEVLIHIGIDTVELDGKGFNLKVKEGTKVTKETEIVEVDFPFLESNGK
CCEEEEEEECCCEEEEEEECCEEEEECCCCEEEEECCCCCCCCCEEEEEEECCEECCCCC
DTDVIVIFTNLKEKPLAITEGLMKVNQEIGTIK
CCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11463916; 7934910 [H]