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The map label for this gene is rlmH [H]

Identifier: 116871690

GI number: 116871690

Start: 295076

End: 295555

Strand: Direct

Name: rlmH [H]

Synonym: lwe0270

Alternate gene names: 116871690

Gene position: 295076-295555 (Clockwise)

Preceding gene: 116871689

Following gene: 116871691

Centisome position: 10.49

GC content: 42.92

Gene sequence:

>480_bases
ATGAATATCCAAATTATAACGGTGGGCAAACTAAAAGAAAAATATTTAGTTCAAGGAATTGCCGAGTATTTAAAACGACT
TACTGCATATGCAAAAGTGACGATTGTTGAAGTTCCGGATGAAAAAGCACCAGAAGTGTTAAGTGATGCGGAAATGAAGC
AAGTGAAAGATAAAGAAGGTGTGCGGATTTTAGCAAAGATTCCAGATGACACCCATGTGATTGCACTAGCTATTGATGGG
AAAATGAAGTCGAGTGAGGAGTTTGCAGCGGATTTGGATAAGCTGGCGACGTATGGTAAAAGTAAGGTGGCGTTTGTGAT
TGGTGGATCGCTGGGGCTTAGTGAGTCGGTGTTGAAGCGGAGTGATGAGCAGATTTCGTTTGGTCGGTTAACGTTACCAC
ATCAGTTGATGAGATTGGTGCTGGTGGAGCAGGTTTACCGGGCATTTCGGATTGTTCGGGGAGAGCCTTATCATAAATAG

Upstream 100 bases:

>100_bases
CGAGGATAGTGTTTTTTTATTTAGAATTATAGATTTTGGTAGTGTTGGATGTGTTCTTTGATGTATGATAAATGTATCAA
TAAAGATAAGGGTGACGTAT

Downstream 100 bases:

>100_bases
AACTTTTTTATCAAAGGAGAATTCAAAATGAAATTTCTAAAAAAATATGGTTTTTACTTTTTAATACTGGCGGTTTTAAG
TGACTTTCTAACGCCTTATA

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MNIQIITVGKLKEKYLVQGIAEYLKRLTAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEGVRILAKIPDDTHVIALAIDG
KMKSSEEFAADLDKLATYGKSKVAFVIGGSLGLSESVLKRSDEQISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK

Sequences:

>Translated_159_residues
MNIQIITVGKLKEKYLVQGIAEYLKRLTAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEGVRILAKIPDDTHVIALAIDG
KMKSSEEFAADLDKLATYGKSKVAFVIGGSLGLSESVLKRSDEQISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK
>Mature_159_residues
MNIQIITVGKLKEKYLVQGIAEYLKRLTAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEGVRILAKIPDDTHVIALAIDG
KMKSSEEFAADLDKLATYGKSKVAFVIGGSLGLSESVLKRSDEQISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA [H]

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family [H]

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=32.0754716981132, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016051
- InterPro:   IPR003742 [H]

Pfam domain/function: PF02590 SPOUT_MTase [H]

EC number: NA

Molecular weight: Translated: 17786; Mature: 17786

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQIITVGKLKEKYLVQGIAEYLKRLTAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEG
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCC
VRILAKIPDDTHVIALAIDGKMKSSEEFAADLDKLATYGKSKVAFVIGGSLGLSESVLKR
CEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
SDEQISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNIQIITVGKLKEKYLVQGIAEYLKRLTAYAKVTIVEVPDEKAPEVLSDAEMKQVKDKEG
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCCC
VRILAKIPDDTHVIALAIDGKMKSSEEFAADLDKLATYGKSKVAFVIGGSLGLSESVLKR
CEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHC
SDEQISFGRLTLPHQLMRLVLVEQVYRAFRIVRGEPYHK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]