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The map label for this gene is mtnE [H]

Identifier: 116871680

GI number: 116871680

Start: 280972

End: 282153

Strand: Direct

Name: mtnE [H]

Synonym: lwe0260

Alternate gene names: 116871680

Gene position: 280972-282153 (Clockwise)

Preceding gene: 116871674

Following gene: 116871681

Centisome position: 9.98

GC content: 39.34

Gene sequence:

>1182_bases
ATGAAAATTTCCAAACGCTTACAAAATTTACCAGATCAGTTTTTCTCTAGCCTTGTAGAAAAAGTTGGAAAAAAGGTGGC
AGAAGGGCATGACGTGATTAACTTAGGTCAAGGAAACCCAGACCAGCCAACACCCAAACATATCGTTCAAGCAATGAAAA
CCGCTTCAGAAAACCCTATGAACCACAAATATTCCTTGTTTCGTGGAAAAAGGGAGCTAAAACAAGCTGCAGCTGATTTT
TATGCGCGCGAATATAATGTGACTATTGATCCAGATACAGAAGTAGCGATTTTATTTGGTACTAAAACGGGCCTAGTGGA
ACTGCCGATGTGTTTAATGGATCCAGGTGATGTGATGCTTTTACCAGACCCGGGTTATCCAGATTATTTGTCGGGCGCTG
TCTTAGGCGAGGTTCAATTTGAAACAATGCCACTTATTGATGAAAATAACTTTTTACCAGATTTCTCTAAAATTCCACCA
GAAATTGCTGAAAAAGCGGAATTAATGTATTTAAATTATCCGAATAACCCAACTGGTGCAGTTGCAACAGCTGACTTTTT
TGAAGAAACGGTTGCTTTTGCCAAAGAAAATAATGTAACAATTGCGCACGATTTTGCTTATGGTGGTATTGGATTTGATG
GTAAGAAACCGATTAGTTTTTTAGAAACACCTGGTGCAAAAGAAGTAGGTATTGAATTATACACACTTTCTAAAACCTAT
AATATGGCAGGATGGCGTGTAGGTTTTGCCGTTGGAAATAAAGAAGTGGTTGAAGCAATAAACCTTCTTCAAGATCATAT
GTATGTAAGTCTTTTTCCTGGAATTCAAGATGCTGCAATCGAAGCGCTCATTGGTAATCAAACATGCGTGACAGAATTAA
ATGCTCGTTATGAAAGTCGCAGAAATGCTTTTATCTCGGCTTGTGATAAAATTGGCTGGAAAACAGTTGCACCAGCTGGT
TCCTTCTTCGCCTGGATGCCCGTGCCTGAGCAGTTTACTAGTAGCGAGTTTGCAGATTATTTATTGAATGAAGTAAGTGT
GGCGGTCGCTGACGGAAGTGGCTTTGGCAAATTTGGGGAAGGTTATGTGAGGGTAGGCCTTTTAATGGATGAGGAACGAT
TAGAAGAAGCTGTAACGCGAATTTCTAAACTTCATTTATTTGATAAAGTGCCACAAAACTAA

Upstream 100 bases:

>100_bases
TTGTCAATGCTTTTTTAAGTTTAAAAAAAATCATACTGGACAAATACTTCAGAAAACTTTACTATTTTAATCAATGAAAC
TTGTAAAGGACGGGTGTAAA

Downstream 100 bases:

>100_bases
GCAAATAGGTTATTTTAAAAGGAAATAGCTTGTAATAATTCAGATAATTACTTGTATGTGTTATAATTGATTAGTAAGTC
TATTCTTAGCATGTTGTTTT

Product: transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVQAMKTASENPMNHKYSLFRGKRELKQAAADF
YAREYNVTIDPDTEVAILFGTKTGLVELPMCLMDPGDVMLLPDPGYPDYLSGAVLGEVQFETMPLIDENNFLPDFSKIPP
EIAEKAELMYLNYPNNPTGAVATADFFEETVAFAKENNVTIAHDFAYGGIGFDGKKPISFLETPGAKEVGIELYTLSKTY
NMAGWRVGFAVGNKEVVEAINLLQDHMYVSLFPGIQDAAIEALIGNQTCVTELNARYESRRNAFISACDKIGWKTVAPAG
SFFAWMPVPEQFTSSEFADYLLNEVSVAVADGSGFGKFGEGYVRVGLLMDEERLEEAVTRISKLHLFDKVPQN

Sequences:

>Translated_393_residues
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVQAMKTASENPMNHKYSLFRGKRELKQAAADF
YAREYNVTIDPDTEVAILFGTKTGLVELPMCLMDPGDVMLLPDPGYPDYLSGAVLGEVQFETMPLIDENNFLPDFSKIPP
EIAEKAELMYLNYPNNPTGAVATADFFEETVAFAKENNVTIAHDFAYGGIGFDGKKPISFLETPGAKEVGIELYTLSKTY
NMAGWRVGFAVGNKEVVEAINLLQDHMYVSLFPGIQDAAIEALIGNQTCVTELNARYESRRNAFISACDKIGWKTVAPAG
SFFAWMPVPEQFTSSEFADYLLNEVSVAVADGSGFGKFGEGYVRVGLLMDEERLEEAVTRISKLHLFDKVPQN
>Mature_393_residues
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVQAMKTASENPMNHKYSLFRGKRELKQAAADF
YAREYNVTIDPDTEVAILFGTKTGLVELPMCLMDPGDVMLLPDPGYPDYLSGAVLGEVQFETMPLIDENNFLPDFSKIPP
EIAEKAELMYLNYPNNPTGAVATADFFEETVAFAKENNVTIAHDFAYGGIGFDGKKPISFLETPGAKEVGIELYTLSKTY
NMAGWRVGFAVGNKEVVEAINLLQDHMYVSLFPGIQDAAIEALIGNQTCVTELNARYESRRNAFISACDKIGWKTVAPAG
SFFAWMPVPEQFTSSEFADYLLNEVSVAVADGSGFGKFGEGYVRVGLLMDEERLEEAVTRISKLHLFDKVPQN

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Homo sapiens, GI95147551, Length=317, Percent_Identity=24.9211356466877, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI169881279, Length=317, Percent_Identity=24.9211356466877, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI56713256, Length=316, Percent_Identity=23.4177215189873, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI56713254, Length=316, Percent_Identity=23.4177215189873, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI169881281, Length=309, Percent_Identity=23.9482200647249, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788722, Length=386, Percent_Identity=37.5647668393782, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1786816, Length=364, Percent_Identity=25.2747252747253, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788627, Length=403, Percent_Identity=24.8138957816377, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994476, Length=393, Percent_Identity=24.1730279898219, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994472, Length=393, Percent_Identity=24.1730279898219, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17567369, Length=318, Percent_Identity=21.6981132075472, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17567663, Length=379, Percent_Identity=24.5382585751979, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322401, Length=394, Percent_Identity=23.0964467005076, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573069, Length=415, Percent_Identity=22.4096385542169, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24646114, Length=415, Percent_Identity=22.4096385542169, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573067, Length=415, Percent_Identity=22.4096385542169, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573065, Length=415, Percent_Identity=22.4096385542169, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.- [C]

Molecular weight: Translated: 43426; Mature: 43426

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVQAMKTASENPM
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
NHKYSLFRGKRELKQAAADFYAREYNVTIDPDTEVAILFGTKTGLVELPMCLMDPGDVML
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCCEEE
LPDPGYPDYLSGAVLGEVQFETMPLIDENNFLPDFSKIPPEIAEKAELMYLNYPNNPTGA
ECCCCCCHHHCCCEEEEEEEEEECEECCCCCCCCHHHCCHHHHCCCEEEEEECCCCCCCC
VATADFFEETVAFAKENNVTIAHDFAYGGIGFDGKKPISFLETPGAKEVGIELYTLSKTY
EEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHCEEEEEEECCC
NMAGWRVGFAVGNKEVVEAINLLQDHMYVSLFPGIQDAAIEALIGNQTCVTELNARYESR
CCCCEEEEEEECCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RNAFISACDKIGWKTVAPAGSFFAWMPVPEQFTSSEFADYLLNEVSVAVADGSGFGKFGE
HHHHHHHHHHCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
GYVRVGLLMDEERLEEAVTRISKLHLFDKVPQN
CEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKISKRLQNLPDQFFSSLVEKVGKKVAEGHDVINLGQGNPDQPTPKHIVQAMKTASENPM
CCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCC
NHKYSLFRGKRELKQAAADFYAREYNVTIDPDTEVAILFGTKTGLVELPMCLMDPGDVML
CCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCCCCCCHHHCCCCCEEE
LPDPGYPDYLSGAVLGEVQFETMPLIDENNFLPDFSKIPPEIAEKAELMYLNYPNNPTGA
ECCCCCCHHHCCCEEEEEEEEEECEECCCCCCCCHHHCCHHHHCCCEEEEEECCCCCCCC
VATADFFEETVAFAKENNVTIAHDFAYGGIGFDGKKPISFLETPGAKEVGIELYTLSKTY
EEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHCEEEEEEECCC
NMAGWRVGFAVGNKEVVEAINLLQDHMYVSLFPGIQDAAIEALIGNQTCVTELNARYESR
CCCCEEEEEEECCHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RNAFISACDKIGWKTVAPAGSFFAWMPVPEQFTSSEFADYLLNEVSVAVADGSGFGKFGE
HHHHHHHHHHCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
GYVRVGLLMDEERLEEAVTRISKLHLFDKVPQN
CEEEEEEEECHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]