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The map label for this gene is gtfA [H]

Identifier: 116871646

GI number: 116871646

Start: 234878

End: 236320

Strand: Direct

Name: gtfA [H]

Synonym: lwe0226

Alternate gene names: 116871646

Gene position: 234878-236320 (Clockwise)

Preceding gene: 116871645

Following gene: 116871647

Centisome position: 8.35

GC content: 32.5

Gene sequence:

>1443_bases
TTGCAAATTAAAAATAAAGCGATGTTAATTACTTATTCTGATAGTCTGGGTCGAAATATGGAAGAACTTTATCAAATGAT
GGAAACTTATTTTGAAGATGTTGTGGGTGGGATTCACTTATTACCATTCTTTCCATCTACTGGAGATCGAGGGTTCGCGC
CAACTGATTATACCATTGTAGATAGTAAGTTAGGATCTTGGGAAATGGTCGAAAAACTAGGTGAAAAATATTATTTAATG
TTTGATTTTATGATTAATCACATTTCTCGAAAATCTCACTTTTTTCAAGATTTTAAAAAAGCGCATGATAACTCAAAATA
TAGAGATATGTTTATTCGGGTAAATGAGTTTTTTCCACCAGGCAGACCAAGTGAAAAAGACTTAGACTTAATCTATAAGC
GAAAAGATAAAGCGCCTTTCCAAGAAGTTGAGTTTGCTGATGGAGAAACAGAGTTAGTCTGGAATACATTTGGTGAAGAA
CAAATTGATTTAGATGTTACAGCAGAAGTTACAAAGGAATTCATTCGCCAAACAATACAAAATATGGCGTCGCACGGTTG
TTCCATCTTACGTCTAGATGCTTTTGCTTATGCTATTAAAAAATTAGAGACCAATGATTTTTTTGTTGAACCTGAAATTT
GGAATTTGCTGGATGAAGTGAAAGCAGAAGCTAAGAAGTATAATATGGAATTATTACCAGAAATTCATGAGCATTATTCA
ATTCAAATGAAAATTGCGAACCATAATTATTATATTTATGATTTTGCGCTACCAATGGTTATGCTTTATTCATTATATAG
CGGACATGCGAATCGTTTAGCGAACTGGTTGAAAATGAGTCCTATGAAGCAATTTACTACATTAGATACACATGATGGCA
TTGGAGTTGTGGATGCCAGAGACCTATTAACAGACGAAGAACTTGATTATACATCAAAAGAATTATACAAAATAGGAGCC
AATGTCAAAAAAGTATATTCCTCCGAGAAATATAACAACTTGGATATTTATCAAATTAATAGTACGTATTATAGTGCTTT
AGGCGATGATGATAAAAGTTATTTGTTGGCGAGAGCGATTCAATGTTTTGCACCAGGAATTCCACAAATATACTATGTTG
GATTACTAGCGGGTAAAAATGATATTGAACTTTTGGAAAAAACGAAAGAAGGACGTAATATCAATCGCCATTATTACACA
GTAGATGAAATTGCCAAGGAAGTAGAAAGACCAGTTGTTAAAGCATTAAGTAAGCTACTTCGATTTAGAAATAATTCTGA
AGCATTTGATTTAGAGGGTAGCATAGAAGTGCAAACACCAAATTCAAATGAAATAGTAATTATTCGCAAAAATAAAACCG
GAAAAATAGTAGCAATCTTAACAGCGAATTTAATGACTAAATCATTCCAAATTACCGAAAACGAAAGAAATATTGCCATT
TAA

Upstream 100 bases:

>100_bases
GATAATTGCCAATGTGGAGTTGCCAAAAATTGTGGAAAATAAATTTATTTTACCCGCTTATACAGGCGCCGTTTTTTCAA
AAATTTTGGAGGTAGAGTGA

Downstream 100 bases:

>100_bases
TTATAAATAGACACGAATGAGGTCTCTCGTTCGTGTTTGTTTGTATTTTCTATTGACAACGGCGCCTGATTATTGTATTA
TTATATAATGCCAAAAGAAT

Product: sucrose phosphorylase

Products: NA

Alternate protein names: Glucosyltransferase-A; GTF-A; Sucrose glucosyltransferase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQIKNKAMLITYSDSLGRNMEELYQMMETYFEDVVGGIHLLPFFPSTGDRGFAPTDYTIVDSKLGSWEMVEKLGEKYYLM
FDFMINHISRKSHFFQDFKKAHDNSKYRDMFIRVNEFFPPGRPSEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEE
QIDLDVTAEVTKEFIRQTIQNMASHGCSILRLDAFAYAIKKLETNDFFVEPEIWNLLDEVKAEAKKYNMELLPEIHEHYS
IQMKIANHNYYIYDFALPMVMLYSLYSGHANRLANWLKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSKELYKIGA
NVKKVYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARAIQCFAPGIPQIYYVGLLAGKNDIELLEKTKEGRNINRHYYT
VDEIAKEVERPVVKALSKLLRFRNNSEAFDLEGSIEVQTPNSNEIVIIRKNKTGKIVAILTANLMTKSFQITENERNIAI

Sequences:

>Translated_480_residues
MQIKNKAMLITYSDSLGRNMEELYQMMETYFEDVVGGIHLLPFFPSTGDRGFAPTDYTIVDSKLGSWEMVEKLGEKYYLM
FDFMINHISRKSHFFQDFKKAHDNSKYRDMFIRVNEFFPPGRPSEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEE
QIDLDVTAEVTKEFIRQTIQNMASHGCSILRLDAFAYAIKKLETNDFFVEPEIWNLLDEVKAEAKKYNMELLPEIHEHYS
IQMKIANHNYYIYDFALPMVMLYSLYSGHANRLANWLKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSKELYKIGA
NVKKVYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARAIQCFAPGIPQIYYVGLLAGKNDIELLEKTKEGRNINRHYYT
VDEIAKEVERPVVKALSKLLRFRNNSEAFDLEGSIEVQTPNSNEIVIIRKNKTGKIVAILTANLMTKSFQITENERNIAI
>Mature_480_residues
MQIKNKAMLITYSDSLGRNMEELYQMMETYFEDVVGGIHLLPFFPSTGDRGFAPTDYTIVDSKLGSWEMVEKLGEKYYLM
FDFMINHISRKSHFFQDFKKAHDNSKYRDMFIRVNEFFPPGRPSEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEE
QIDLDVTAEVTKEFIRQTIQNMASHGCSILRLDAFAYAIKKLETNDFFVEPEIWNLLDEVKAEAKKYNMELLPEIHEHYS
IQMKIANHNYYIYDFALPMVMLYSLYSGHANRLANWLKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSKELYKIGA
NVKKVYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARAIQCFAPGIPQIYYVGLLAGKNDIELLEKTKEGRNINRHYYT
VDEIAKEVERPVVKALSKLLRFRNNSEAFDLEGSIEVQTPNSNEIVIIRKNKTGKIVAILTANLMTKSFQITENERNIAI

Specific function: Intracellular catabolism of sucrose. Production of extracellular glucans, that are thought to play a key role in the development of the dental plaque because of their ability to adhere to smooth surfaces and mediate the aggregation of bacterial cells and

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sucrose phosphorylase family [H]

Homologues:

Organism=Escherichia coli, GI87081873, Length=506, Percent_Identity=27.2727272727273, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR016377
- InterPro:   IPR022527 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =2.4.1.7 [H]

Molecular weight: Translated: 55770; Mature: 55770

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKNKAMLITYSDSLGRNMEELYQMMETYFEDVVGGIHLLPFFPSTGDRGFAPTDYTIV
CCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEE
DSKLGSWEMVEKLGEKYYLMFDFMINHISRKSHFFQDFKKAHDNSKYRDMFIRVNEFFPP
CCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCC
GRPSEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEEQIDLDVTAEVTKEFIRQTIQ
CCCCCCCHHHHEECCCCCCHHHEECCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHH
NMASHGCSILRLDAFAYAIKKLETNDFFVEPEIWNLLDEVKAEAKKYNMELLPEIHEHYS
HHHHCCCEEEEHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCEE
IQMKIANHNYYIYDFALPMVMLYSLYSGHANRLANWLKMSPMKQFTTLDTHDGIGVVDAR
EEEEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCCCEEEHH
DLLTDEELDYTSKELYKIGANVKKVYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARAI
HHCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCCEEEEEECCHHHHHCCCCCHHHHHHHHH
QCFAPGIPQIYYVGLLAGKNDIELLEKTKEGRNINRHYYTVDEIAKEVERPVVKALSKLL
HHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RFRNNSEAFDLEGSIEVQTPNSNEIVIIRKNKTGKIVAILTANLMTKSFQITENERNIAI
HHCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCEEEEEEEHHHHCEEEEECCCCCCCC
>Mature Secondary Structure
MQIKNKAMLITYSDSLGRNMEELYQMMETYFEDVVGGIHLLPFFPSTGDRGFAPTDYTIV
CCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCEEE
DSKLGSWEMVEKLGEKYYLMFDFMINHISRKSHFFQDFKKAHDNSKYRDMFIRVNEFFPP
CCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCC
GRPSEKDLDLIYKRKDKAPFQEVEFADGETELVWNTFGEEQIDLDVTAEVTKEFIRQTIQ
CCCCCCCHHHHEECCCCCCHHHEECCCCCEEEEEECCCCCEEEEEEEHHHHHHHHHHHHH
NMASHGCSILRLDAFAYAIKKLETNDFFVEPEIWNLLDEVKAEAKKYNMELLPEIHEHYS
HHHHCCCEEEEHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHCCHHHHHHHHCCEE
IQMKIANHNYYIYDFALPMVMLYSLYSGHANRLANWLKMSPMKQFTTLDTHDGIGVVDAR
EEEEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHCCCCCCCCCCEEEHH
DLLTDEELDYTSKELYKIGANVKKVYSSEKYNNLDIYQINSTYYSALGDDDKSYLLARAI
HHCCCCCCCCHHHHHHHHCCHHHHHHCCCCCCCEEEEEECCHHHHHCCCCCHHHHHHHHH
QCFAPGIPQIYYVGLLAGKNDIELLEKTKEGRNINRHYYTVDEIAKEVERPVVKALSKLL
HHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHH
RFRNNSEAFDLEGSIEVQTPNSNEIVIIRKNKTGKIVAILTANLMTKSFQITENERNIAI
HHCCCCCEEECCCEEEEECCCCCEEEEEEECCCCCEEEEEEEHHHHCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3194228; 2967248; 12397186; 2971020 [H]