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The map label for this gene is blaI [H]

Identifier: 116871635

GI number: 116871635

Start: 223510

End: 223896

Strand: Direct

Name: blaI [H]

Synonym: lwe0215

Alternate gene names: 116871635

Gene position: 223510-223896 (Clockwise)

Preceding gene: 116871634

Following gene: 116871636

Centisome position: 7.94

GC content: 30.23

Gene sequence:

>387_bases
ATGTCAATTAAAGCTATATCGAAATCTGAACTAGAAATCATGAAAATAATTTGGGAATACGGCAGAGCTGTTCAGTATTC
AGATGTTGCCATGAAACTTAGTGAAAAAGAAGTATTATGGAAGAAAAACACCATTCTTACTTTTTTAGCACGCTTAGTTG
AAAAAAATTTACTTCGAGTTAAAAAAATTGGACGTAAAAATGAATATTATGCACTTGTTAGTGAAAATGAATATTTGGAA
CTGCAAACAGAAACATTTTTAGAAGAGATTTATGAGGGGGATGTAAAAGGATTAATCACGAATTTAGTACAGAATGACTT
AATTTCTTCGGATGAACTTGATGATTTACAACAATTTTGGAAAAGGATGAAGTCACCTAATGAATGA

Upstream 100 bases:

>100_bases
AAGGAATGAAGTCGGAAATAATCCTTTAGAAGGCTTTTTTTTATTGCCGTTACATGCTAGTATTAGTAAGTGAGTTAGTT
AGTTTTCTGGAGGTTTATAC

Downstream 100 bases:

>100_bases
ACTGCTTAAGATGGTCTTATCCATGGCCCTCACTTCACTAGTGTTAATACCATGTATATGGATATTTGGAAAAGCATTTA
ATCGCTATTTAAGTAAGAAA

Product: transcriptional regulator, putative

Products: NA

Alternate protein names: Beta-lactamase repressor protein; Regulatory protein BlaI [H]

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSIKAISKSELEIMKIIWEYGRAVQYSDVAMKLSEKEVLWKKNTILTFLARLVEKNLLRVKKIGRKNEYYALVSENEYLE
LQTETFLEEIYEGDVKGLITNLVQNDLISSDELDDLQQFWKRMKSPNE

Sequences:

>Translated_128_residues
MSIKAISKSELEIMKIIWEYGRAVQYSDVAMKLSEKEVLWKKNTILTFLARLVEKNLLRVKKIGRKNEYYALVSENEYLE
LQTETFLEEIYEGDVKGLITNLVQNDLISSDELDDLQQFWKRMKSPNE
>Mature_127_residues
SIKAISKSELEIMKIIWEYGRAVQYSDVAMKLSEKEVLWKKNTILTFLARLVEKNLLRVKKIGRKNEYYALVSENEYLEL
QTETFLEEIYEGDVKGLITNLVQNDLISSDELDDLQQFWKRMKSPNE

Specific function: Transcriptional repressor that constitutively blocks expression of beta-lactamase. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer [H]

COG id: COG3682

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BlaI transcriptional regulatory family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005650
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03965 Pencillinase_R [H]

EC number: NA

Molecular weight: Translated: 15112; Mature: 14980

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKAISKSELEIMKIIWEYGRAVQYSDVAMKLSEKEVLWKKNTILTFLARLVEKNLLRV
CCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIGRKNEYYALVSENEYLELQTETFLEEIYEGDVKGLITNLVQNDLISSDELDDLQQFW
HHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
KRMKSPNE
HHHCCCCC
>Mature Secondary Structure 
SIKAISKSELEIMKIIWEYGRAVQYSDVAMKLSEKEVLWKKNTILTFLARLVEKNLLRV
CCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIGRKNEYYALVSENEYLELQTETFLEEIYEGDVKGLITNLVQNDLISSDELDDLQQFW
HHCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
KRMKSPNE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3096969; 3305074; 3260234; 3040663; 3498148 [H]