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The map label for this gene is dasR [H]

Identifier: 116871445

GI number: 116871445

Start: 25017

End: 25757

Strand: Direct

Name: dasR [H]

Synonym: lwe0023

Alternate gene names: 116871445

Gene position: 25017-25757 (Clockwise)

Preceding gene: 116871443

Following gene: 116871446

Centisome position: 0.89

GC content: 31.17

Gene sequence:

>741_bases
ATGTCAGAACCAAAATATGCGATTATAATCAGTGATATCAAGCGATTAATCAATAATGGAACTTTCAAGCCAGGAGAAAA
AATTTATTCCGAAGATGAACTTAAAAAGAAATATAATGTTAGTAATACAACAGTTGTTCGCGCTTTACATGAACTTGTAA
GAGCAGGGATTTTAGCGCGTTATCAAGGAAAAGGTACTTACGTTAGTAAATCCATTATCAATGAGGAAGTTATTTTTAAT
GAATATACAACAGTGCCTAACGGTAAATTTAATCGAAAAACAAAAATTACTAATGAGCATACGAAAGTTTTGGCTATTAA
CGAAATTCAAGATGAGCGAATTGCTAAAAATCTGCAAATCCCACCTGAAAACATGATTGTTCATTTTCAGCGGATTCGTC
TTATAGATGACGTACCTTGGACAGTTCAAAACAACTATATGGCAAAATCCAATTTAATTAACGTCGATTTAACGAATCTG
GAAAAGTTTAATTCTTTATCGGAAGTAATTAAAGAGCTTTATGGTATAAATATTCTGCATGAGGCGATGAAAGAGCGCAT
TCAAGTTGAATTTCCAGTAAAAGACAAGAATAATTTTAAATTACTTGAAATCGACACAGAATTACCACTTTATCACATTG
AAAGAATCACTTATGTACCAGAGGGAAAGCCCTATGAATATATTGAAAGCTATTTACGACATAATTTTTACTCTATTGAA
ATTGAAAAGAAAAAACAATAG

Upstream 100 bases:

>100_bases
TTAGCAAGCGAATTCAAGTTTGCAATCAAAAATGTATCATGGTACCATTAACCTAAAACTTAACGTGTTAACACGCGGGA
TGCTTTAGGGGGAACTAACG

Downstream 100 bases:

>100_bases
AAAGGAGATCATTACTTGAATTATATTATCGCAGCACATGGCCGGTACGCGCAGGAAGTAAAAAATAGCTGCCAGATGAT
TACCGGCCAAACAACGAATA

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSEPKYAIIISDIKRLINNGTFKPGEKIYSEDELKKKYNVSNTTVVRALHELVRAGILARYQGKGTYVSKSIINEEVIFN
EYTTVPNGKFNRKTKITNEHTKVLAINEIQDERIAKNLQIPPENMIVHFQRIRLIDDVPWTVQNNYMAKSNLINVDLTNL
EKFNSLSEVIKELYGINILHEAMKERIQVEFPVKDKNNFKLLEIDTELPLYHIERITYVPEGKPYEYIESYLRHNFYSIE
IEKKKQ

Sequences:

>Translated_246_residues
MSEPKYAIIISDIKRLINNGTFKPGEKIYSEDELKKKYNVSNTTVVRALHELVRAGILARYQGKGTYVSKSIINEEVIFN
EYTTVPNGKFNRKTKITNEHTKVLAINEIQDERIAKNLQIPPENMIVHFQRIRLIDDVPWTVQNNYMAKSNLINVDLTNL
EKFNSLSEVIKELYGINILHEAMKERIQVEFPVKDKNNFKLLEIDTELPLYHIERITYVPEGKPYEYIESYLRHNFYSIE
IEKKKQ
>Mature_245_residues
SEPKYAIIISDIKRLINNGTFKPGEKIYSEDELKKKYNVSNTTVVRALHELVRAGILARYQGKGTYVSKSIINEEVIFNE
YTTVPNGKFNRKTKITNEHTKVLAINEIQDERIAKNLQIPPENMIVHFQRIRLIDDVPWTVQNNYMAKSNLINVDLTNLE
KFNSLSEVIKELYGINILHEAMKERIQVEFPVKDKNNFKLLEIDTELPLYHIERITYVPEGKPYEYIESYLRHNFYSIEI
EKKKQ

Specific function: Global regulator that is part of the nutrient-sensing system. In the absence of N-acetylglucosamine 6-P, represses the phosphotransferase system (PTS) specific for the uptake of N- acetylglucosamine (PTSNag), and genes involved in the metabolism of chitin

COG id: COG2188

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082252, Length=243, Percent_Identity=24.6913580246914, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786950, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000524
- InterPro:   IPR011663
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00392 GntR; PF07702 UTRA [H]

EC number: NA

Molecular weight: Translated: 28908; Mature: 28776

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPKYAIIISDIKRLINNGTFKPGEKIYSEDELKKKYNVSNTTVVRALHELVRAGILAR
CCCCCEEEEHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEE
YQGKGTYVSKSIINEEVIFNEYTTVPNGKFNRKTKITNEHTKVLAINEIQDERIAKNLQI
ECCCCCEEHHHHHCHHHEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCC
PPENMIVHFQRIRLIDDVPWTVQNNYMAKSNLINVDLTNLEKFNSLSEVIKELYGINILH
CCHHHHEEHHHHHHHCCCCCEECCCEEECCCEEEEEHHCHHHHHHHHHHHHHHHCCHHHH
EAMKERIQVEFPVKDKNNFKLLEIDTELPLYHIERITYVPEGKPYEYIESYLRHNFYSIE
HHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCEEEEE
IEKKKQ
EECCCC
>Mature Secondary Structure 
SEPKYAIIISDIKRLINNGTFKPGEKIYSEDELKKKYNVSNTTVVRALHELVRAGILAR
CCCCEEEEHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHEE
YQGKGTYVSKSIINEEVIFNEYTTVPNGKFNRKTKITNEHTKVLAINEIQDERIAKNLQI
ECCCCCEEHHHHHCHHHEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHCCCC
PPENMIVHFQRIRLIDDVPWTVQNNYMAKSNLINVDLTNLEKFNSLSEVIKELYGINILH
CCHHHHEEHHHHHHHCCCCCEECCCEEECCCEEEEEHHCHHHHHHHHHHHHHHHCCHHHH
EAMKERIQVEFPVKDKNNFKLLEIDTELPLYHIERITYVPEGKPYEYIESYLRHNFYSIE
HHHHHHEEEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHCCEEEEE
IEKKKQ
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]