Click here to switch to the map view.

The map label for this gene is alkK [H]

Identifier: 116696458

GI number: 116696458

Start: 2849280

End: 2850926

Strand: Direct

Name: alkK [H]

Synonym: H16_B2522

Alternate gene names: 116696458

Gene position: 2849280-2850926 (Clockwise)

Preceding gene: 116696457

Following gene: 116696463

Centisome position: 97.83

GC content: 66.91

Gene sequence:

>1647_bases
ATGCAACCAAACCTTGTGCCTGCGGCGCCGCAGGCTTATGCCTATCCCTTGCTCGTCAGGCAACTGCTGCTCAATTCGCT
GTCGCTCTACGGCGACCAGCAGATCACGTACCGCGGGCAACTGCGCCATAGCTATCGCGACTTCCACCGGCGTGTCGGCC
GGCTGGCTTCAGCGCTGGCGGCGCAGGGGGTGGCGCACGGCACCACCGTGGCGGTGATGGACTGGGACAGCCACCGCTAC
CTGGAATGCTATTTCGGCGTGCCGATGATGGGCGCGACGCTGTTCACGGTCAACGTGCGGCTGTCGGCGCAGCAAATCCT
GTACACGCTGAACGATTCCGGCGCCGAGGTGGTGCTGCTCCATCCCGACTTCCTGCCGGTGATGGAAGAGATCCGCGGCC
AGCTGACCAGCGTGCGCAGCTTTGTCCTGCTGGCCGACGGCCAGCACGTGCCGCCGACATCTCTGCCGTTCTGCGGCGAG
TACGAGACGCTGCTGAGCGCGGCCTCGCCCGACTTCGACTTCCCCGAGTTCGACGAGAACACCCGCGCCGCCACCTTCTA
CACGACGGGCACCACCGGCGATCCCAAGGGCGTGTGCTACAGCCATCGCGATATCGTGCTGCATGCGCTGGCCTCGGCCA
CCAGCCTGTGCGCGCCGCGCGAAGGACAGCGCCTGCATCGGGAAGACGTCTACATGCCGATCACGCCGATGTTCCACGTG
CTCGCCTGGGGCATCCCGTATGTCGCGGTCATGCTCGGGCTGCGCATCGTGCTGCCGGGGCGCTATGCGCCGGACATGCT
GCTGCAGTTGCGCGAGACCGAGCGGGTCACGTTCTCGCACTGCGTGCCGACCTTGCTGCAGATGCTGTTGCAGGCGGCGC
AGGCAAGCGGCCAGGACTTGTCGGGCTGGAAGCTCATCATCGGCGGCTCGGCGCTGCCGCCGGCGTTGTGCGAGGCTGCC
CTCGAGCGCGGCATGGATGTGTTTGCCGGCTATGGCATGTCGGAGACCGGCCCCATCGTCGCGCTGGCACAGTTGCCGCC
AGGCGACGCACATCGCGACCATGAGACCGAAGTGCGCATGCGCTGCTCGACGGGGCGGCCGGTGGCGATGGTGGACTTCC
GCCTGGTGGATGAATCGATGCACGAGGTGCCGCGCGACGGCCAGGCGCGCGGCGAGATCGTGCTGCGCGCACCGTTCCTG
ACCAGGGCATACCATGGCAAGCCCGAGGCATCGGCCGAGCTGTGGGCCGGCGGCTACCTGCATACGCAGGACATCGCGGT
AATGGGGGCCGACGGCTTCGTGCAGATCGTCGACCGGATCAAGGACGTGATCAAGACCGGCGGCGAATGGGTCTCGTCGA
TCGAGGTCGAGAGCCTGGTCACGCAGGTGCCGGGCGTGCAGGAGTGCGCGGTCATCGGCGTGCCGGACGCCAGGTGGGGC
GAGCGGCCGATGGCATTCGTGGTGCGCAAGCCTGGCACAACGGTGACCGCGGATACGATTCGTACGTCGCTGCTGGCGCG
CGTCGAGGCCAACCGGCTCAGCAAATATGCGGTGCCGGAATCCGAGCGGATCCTGTTTGTCGACGAGATCCCGAAGACCA
GCGTCGGCAAGATCGACAAGAAGCGCTTGCGCAGCACCGGCGCCTGA

Upstream 100 bases:

>100_bases
GCTGCACCCAGCATTACGCAGTGCAGGACTATGCGCCCACGGTGCGCAAAATGGAGCGCGCCGGCATCATCGGCGACGTG
CCGTTCTGACGGAGGCAGAC

Downstream 100 bases:

>100_bases
GCCGGCAGCAGGAAGAAGAAAGGGCAGCCTCGGATGAGGCTGCCCTTTTGCGTTGCCGGGAACTGACTCAGGGCTGGATA
TTGAACGCCTTGACGATGCC

Product: medium-chain-fatty-acid-CoA ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MQPNLVPAAPQAYAYPLLVRQLLLNSLSLYGDQQITYRGQLRHSYRDFHRRVGRLASALAAQGVAHGTTVAVMDWDSHRY
LECYFGVPMMGATLFTVNVRLSAQQILYTLNDSGAEVVLLHPDFLPVMEEIRGQLTSVRSFVLLADGQHVPPTSLPFCGE
YETLLSAASPDFDFPEFDENTRAATFYTTGTTGDPKGVCYSHRDIVLHALASATSLCAPREGQRLHREDVYMPITPMFHV
LAWGIPYVAVMLGLRIVLPGRYAPDMLLQLRETERVTFSHCVPTLLQMLLQAAQASGQDLSGWKLIIGGSALPPALCEAA
LERGMDVFAGYGMSETGPIVALAQLPPGDAHRDHETEVRMRCSTGRPVAMVDFRLVDESMHEVPRDGQARGEIVLRAPFL
TRAYHGKPEASAELWAGGYLHTQDIAVMGADGFVQIVDRIKDVIKTGGEWVSSIEVESLVTQVPGVQECAVIGVPDARWG
ERPMAFVVRKPGTTVTADTIRTSLLARVEANRLSKYAVPESERILFVDEIPKTSVGKIDKKRLRSTGA

Sequences:

>Translated_548_residues
MQPNLVPAAPQAYAYPLLVRQLLLNSLSLYGDQQITYRGQLRHSYRDFHRRVGRLASALAAQGVAHGTTVAVMDWDSHRY
LECYFGVPMMGATLFTVNVRLSAQQILYTLNDSGAEVVLLHPDFLPVMEEIRGQLTSVRSFVLLADGQHVPPTSLPFCGE
YETLLSAASPDFDFPEFDENTRAATFYTTGTTGDPKGVCYSHRDIVLHALASATSLCAPREGQRLHREDVYMPITPMFHV
LAWGIPYVAVMLGLRIVLPGRYAPDMLLQLRETERVTFSHCVPTLLQMLLQAAQASGQDLSGWKLIIGGSALPPALCEAA
LERGMDVFAGYGMSETGPIVALAQLPPGDAHRDHETEVRMRCSTGRPVAMVDFRLVDESMHEVPRDGQARGEIVLRAPFL
TRAYHGKPEASAELWAGGYLHTQDIAVMGADGFVQIVDRIKDVIKTGGEWVSSIEVESLVTQVPGVQECAVIGVPDARWG
ERPMAFVVRKPGTTVTADTIRTSLLARVEANRLSKYAVPESERILFVDEIPKTSVGKIDKKRLRSTGA
>Mature_548_residues
MQPNLVPAAPQAYAYPLLVRQLLLNSLSLYGDQQITYRGQLRHSYRDFHRRVGRLASALAAQGVAHGTTVAVMDWDSHRY
LECYFGVPMMGATLFTVNVRLSAQQILYTLNDSGAEVVLLHPDFLPVMEEIRGQLTSVRSFVLLADGQHVPPTSLPFCGE
YETLLSAASPDFDFPEFDENTRAATFYTTGTTGDPKGVCYSHRDIVLHALASATSLCAPREGQRLHREDVYMPITPMFHV
LAWGIPYVAVMLGLRIVLPGRYAPDMLLQLRETERVTFSHCVPTLLQMLLQAAQASGQDLSGWKLIIGGSALPPALCEAA
LERGMDVFAGYGMSETGPIVALAQLPPGDAHRDHETEVRMRCSTGRPVAMVDFRLVDESMHEVPRDGQARGEIVLRAPFL
TRAYHGKPEASAELWAGGYLHTQDIAVMGADGFVQIVDRIKDVIKTGGEWVSSIEVESLVTQVPGVQECAVIGVPDARWG
ERPMAFVVRKPGTTVTADTIRTSLLARVEANRLSKYAVPESERILFVDEIPKTSVGKIDKKRLRSTGA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=579, Percent_Identity=27.6338514680484, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI187761343, Length=579, Percent_Identity=27.6338514680484, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=25.3759398496241, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI38505220, Length=454, Percent_Identity=27.5330396475771, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI115511026, Length=520, Percent_Identity=25.1923076923077, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI42544132, Length=465, Percent_Identity=24.7311827956989, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI122937307, Length=525, Percent_Identity=25.7142857142857, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI58082049, Length=525, Percent_Identity=23.4285714285714, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI157311624, Length=524, Percent_Identity=23.0916030534351, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI157311622, Length=524, Percent_Identity=23.0916030534351, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI28416953, Length=542, Percent_Identity=23.9852398523985, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI13236579, Length=509, Percent_Identity=24.1650294695481, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI38524616, Length=513, Percent_Identity=24.1715399610136, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI13375727, Length=557, Percent_Identity=22.9802513464991, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=30.2536231884058, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI221142682, Length=513, Percent_Identity=25.7309941520468, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI145693145, Length=367, Percent_Identity=30.2452316076294, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1786810, Length=523, Percent_Identity=24.282982791587, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788595, Length=449, Percent_Identity=24.0534521158129, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1790505, Length=540, Percent_Identity=22.4074074074074, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1786529, Length=545, Percent_Identity=24.4036697247706, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786801, Length=446, Percent_Identity=25.5605381165919, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17559526, Length=478, Percent_Identity=27.4058577405858, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17538037, Length=533, Percent_Identity=23.2645403377111, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=560, Percent_Identity=25.1785714285714, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996755, Length=542, Percent_Identity=25.0922509225092, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=538, Percent_Identity=23.9776951672862, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531443, Length=515, Percent_Identity=23.3009708737864, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=590, Percent_Identity=22.7118644067797, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994694, Length=590, Percent_Identity=22.7118644067797, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=544, Percent_Identity=22.7941176470588, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560140, Length=478, Percent_Identity=22.5941422594142, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=369, Percent_Identity=24.9322493224932, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17558820, Length=374, Percent_Identity=27.2727272727273, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI32563687, Length=513, Percent_Identity=23.5867446393762, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556552, Length=368, Percent_Identity=26.0869565217391, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17541856, Length=410, Percent_Identity=25.609756097561, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=386, Percent_Identity=26.9430051813472, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319264, Length=552, Percent_Identity=24.2753623188406, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323182, Length=142, Percent_Identity=29.5774647887324, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21355181, Length=539, Percent_Identity=24.6753246753247, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24581924, Length=588, Percent_Identity=25.8503401360544, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859661, Length=374, Percent_Identity=26.4705882352941, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19922652, Length=552, Percent_Identity=23.0072463768116, Blast_Score=112, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356947, Length=504, Percent_Identity=22.4206349206349, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24653035, Length=515, Percent_Identity=22.5242718446602, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20130357, Length=371, Percent_Identity=24.5283018867925, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648260, Length=371, Percent_Identity=24.7978436657682, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356441, Length=362, Percent_Identity=25.414364640884, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI161076582, Length=522, Percent_Identity=20.4980842911877, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24667955, Length=602, Percent_Identity=23.2558139534884, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648257, Length=369, Percent_Identity=23.8482384823848, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI62472339, Length=564, Percent_Identity=22.6950354609929, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667959, Length=404, Percent_Identity=25.2475247524752, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21358303, Length=341, Percent_Identity=25.2199413489736, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=361, Percent_Identity=22.7146814404432, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24648255, Length=368, Percent_Identity=23.0978260869565, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281365686, Length=149, Percent_Identity=25.503355704698, Blast_Score=70, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 60254; Mature: 60254

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPNLVPAAPQAYAYPLLVRQLLLNSLSLYGDQQITYRGQLRHSYRDFHRRVGRLASALA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AQGVAHGTTVAVMDWDSHRYLECYFGVPMMGATLFTVNVRLSAQQILYTLNDSGAEVVLL
HCCCCCCCEEEEEECCCCCEEEEEECCCHHCCEEEEEEEEEEHHHEEEEECCCCCEEEEE
HPDFLPVMEEIRGQLTSVRSFVLLADGQHVPPTSLPFCGEYETLLSAASPDFDFPEFDEN
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
TRAATFYTTGTTGDPKGVCYSHRDIVLHALASATSLCAPREGQRLHREDVYMPITPMFHV
CCEEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHH
LAWGIPYVAVMLGLRIVLPGRYAPDMLLQLRETERVTFSHCVPTLLQMLLQAAQASGQDL
HHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
SGWKLIIGGSALPPALCEAALERGMDVFAGYGMSETGPIVALAQLPPGDAHRDHETEVRM
CCCEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEE
RCSTGRPVAMVDFRLVDESMHEVPRDGQARGEIVLRAPFLTRAYHGKPEASAELWAGGYL
EECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCCCCCCCCHHCCCCEE
HTQDIAVMGADGFVQIVDRIKDVIKTGGEWVSSIEVESLVTQVPGVQECAVIGVPDARWG
EECCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
ERPMAFVVRKPGTTVTADTIRTSLLARVEANRLSKYAVPESERILFVDEIPKTSVGKIDK
CCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHH
KRLRSTGA
HHHHCCCC
>Mature Secondary Structure
MQPNLVPAAPQAYAYPLLVRQLLLNSLSLYGDQQITYRGQLRHSYRDFHRRVGRLASALA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AQGVAHGTTVAVMDWDSHRYLECYFGVPMMGATLFTVNVRLSAQQILYTLNDSGAEVVLL
HCCCCCCCEEEEEECCCCCEEEEEECCCHHCCEEEEEEEEEEHHHEEEEECCCCCEEEEE
HPDFLPVMEEIRGQLTSVRSFVLLADGQHVPPTSLPFCGEYETLLSAASPDFDFPEFDEN
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
TRAATFYTTGTTGDPKGVCYSHRDIVLHALASATSLCAPREGQRLHREDVYMPITPMFHV
CCEEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHH
LAWGIPYVAVMLGLRIVLPGRYAPDMLLQLRETERVTFSHCVPTLLQMLLQAAQASGQDL
HHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
SGWKLIIGGSALPPALCEAALERGMDVFAGYGMSETGPIVALAQLPPGDAHRDHETEVRM
CCCEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCEEE
RCSTGRPVAMVDFRLVDESMHEVPRDGQARGEIVLRAPFLTRAYHGKPEASAELWAGGYL
EECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHCCCCCCCCHHCCCCEE
HTQDIAVMGADGFVQIVDRIKDVIKTGGEWVSSIEVESLVTQVPGVQECAVIGVPDARWG
EECCEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
ERPMAFVVRKPGTTVTADTIRTSLLARVEANRLSKYAVPESERILFVDEIPKTSVGKIDK
CCCEEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHH
KRLRSTGA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]