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The map label for this gene is pucI [H]

Identifier: 116696417

GI number: 116696417

Start: 2805885

End: 2807450

Strand: Direct

Name: pucI [H]

Synonym: H16_B2481

Alternate gene names: 116696417

Gene position: 2805885-2807450 (Clockwise)

Preceding gene: 116696412

Following gene: 116696418

Centisome position: 96.34

GC content: 64.24

Gene sequence:

>1566_bases
ATGTCGCCTATCGTCAAGACACACATCTGCCAGGAGACTCAGATGATGCAAACGACCGCAACCCCCGCCGACGCACCGCA
GGTGCAGGCCGGGCATGCCGCACACGGCAACGCCCTGATCAAGCCGGGGTATGACGAACGCCTGACCAATGAGGACCTGG
CGCCGCTGCGCAAGCAGACCTGGGGCACCTACAACATCTTTGCCTTCTGGATGTCGGACGTGCACAGCGTGGGCGGCTAT
ATCACCGCCGGCAGCCTGTTCGCGCTGGGGCTGACCAGCTGGCAGGTGCTGGTGTCGCTGCTGGTCGGCATCCTGATCGT
GCAGTACTTCTGCAACCTGGTGGCCAAGCCCAGCCAGGTGACCGGCACGCCTTATCCGGTGATCTGCCGGGCCTCGTTCG
GCGTGCTCGGCGCCAATATCCCGGCGGTGATCCGCGGGCTCATCGCAGTGGCGTGGTACGGCATCCAGACCTACCTGGCT
TCCAGCGCCTTCCTGGTGCTGGCGCTGCACTTCTTCCCCGCGCTGGCGCCCTATGCCGACGTGAAGCTGCACGGCTTTGC
CGGACTCTCCACGCTGGGCTGGGCCGCATTCATGGTGCTGTGGGTGTTGCAGGCGCTGGTGTTCTGGACCGGCATGGAGA
CCATCCGCAAGTTCATCGACTGGGCCGGCCCGGCGGTGTACGTGGTTATGATCGCGCTGGCGATCTGGCTGGTGAACAAG
GCCGGCTGGGAGAACGTCAATTTCACGCTGAGCTTCGTCAAGTACACCGGCTGGGAAGCGGTGCCGGTGATGCTGAGCGC
GATCGCGCTGGTGGTGTCGTATTTCTCCGGCCCGATGCTGAACTTCGGCGACTTCTCGCGCTATGCCAAGGACTTCAAGT
CGGTCAAGCGCGGCAACTTCTGGGGCCTGCCGGTCAACTTCCTGGGCTTCTCGCTGCTGACCGTGATCACGACCTCGGCC
ACGCTGCCGGTGTTCGGCAAGCTGATCACCGACCCGGTGGAGACCGTCAGCCATATCGACAGCACCTTCGCCATGCTGCT
GGGCGCTTTCACCTTCATGACCGCGACCATCGGCATCAATATCGTCGCCAACTTCGTTTCGCCGGCATTCGACTTCTCCA
ACGTCTCGCCCAAGCACATCAGCTGGCGCACCGGCGGCATGATCGCGGCCGTGGCTTCGATCTTCATCACGCCGTGGAAC
CTGTACAACAATCCGGCGGTGATCCACTACACGCTGGACGTGCTGGGCGCCTTCATCGGCCCGCTGTTCGGCATCCTGAT
TGCGGACTACTACCTGGTGCGGCGCCAGCATGTCGACGTCGACGACCTGTACACGCTGAACCCGCGCGGCCGCTACTGGT
ACCGCAACGGCTTCAACCCGGCGGCGGTGGCCACCATGATCCCGGCGGCCATCATCCCGATCCTGTGCGTGGTGGTGCCG
GCCTGGCAGTCCGCGGCCAACTACAGCTGGTTTATCGGCATGGGCATTGCGCTGGTGCTGTACCGGCAACTGGCGCCGCG
CATGATGCCCGGCCACTTCGGCAGCCGTGCCGCCAGCCAGGCCTGA

Upstream 100 bases:

>100_bases
TCTACAACATCGTCTACACACTAAATCAATTTTGTAGACAATTTTGTAGTCAATAAAAAGCAGCTTTCCACCGGCGCACA
CCAAAGAGCCCAACGGCCGC

Downstream 100 bases:

>100_bases
GCCCCGGAACCATTCTTTCGACTGCACGATTTCGACCGCACTTAGCAGAGCCGCGTCTATGAAGCTGAAGATCATCAACC
CCAATACCACCCAGAGCATG

Product: NCS1 nucleoside transporter

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSPIVKTHICQETQMMQTTATPADAPQVQAGHAAHGNALIKPGYDERLTNEDLAPLRKQTWGTYNIFAFWMSDVHSVGGY
ITAGSLFALGLTSWQVLVSLLVGILIVQYFCNLVAKPSQVTGTPYPVICRASFGVLGANIPAVIRGLIAVAWYGIQTYLA
SSAFLVLALHFFPALAPYADVKLHGFAGLSTLGWAAFMVLWVLQALVFWTGMETIRKFIDWAGPAVYVVMIALAIWLVNK
AGWENVNFTLSFVKYTGWEAVPVMLSAIALVVSYFSGPMLNFGDFSRYAKDFKSVKRGNFWGLPVNFLGFSLLTVITTSA
TLPVFGKLITDPVETVSHIDSTFAMLLGAFTFMTATIGINIVANFVSPAFDFSNVSPKHISWRTGGMIAAVASIFITPWN
LYNNPAVIHYTLDVLGAFIGPLFGILIADYYLVRRQHVDVDDLYTLNPRGRYWYRNGFNPAAVATMIPAAIIPILCVVVP
AWQSAANYSWFIGMGIALVLYRQLAPRMMPGHFGSRAASQA

Sequences:

>Translated_521_residues
MSPIVKTHICQETQMMQTTATPADAPQVQAGHAAHGNALIKPGYDERLTNEDLAPLRKQTWGTYNIFAFWMSDVHSVGGY
ITAGSLFALGLTSWQVLVSLLVGILIVQYFCNLVAKPSQVTGTPYPVICRASFGVLGANIPAVIRGLIAVAWYGIQTYLA
SSAFLVLALHFFPALAPYADVKLHGFAGLSTLGWAAFMVLWVLQALVFWTGMETIRKFIDWAGPAVYVVMIALAIWLVNK
AGWENVNFTLSFVKYTGWEAVPVMLSAIALVVSYFSGPMLNFGDFSRYAKDFKSVKRGNFWGLPVNFLGFSLLTVITTSA
TLPVFGKLITDPVETVSHIDSTFAMLLGAFTFMTATIGINIVANFVSPAFDFSNVSPKHISWRTGGMIAAVASIFITPWN
LYNNPAVIHYTLDVLGAFIGPLFGILIADYYLVRRQHVDVDDLYTLNPRGRYWYRNGFNPAAVATMIPAAIIPILCVVVP
AWQSAANYSWFIGMGIALVLYRQLAPRMMPGHFGSRAASQA
>Mature_520_residues
SPIVKTHICQETQMMQTTATPADAPQVQAGHAAHGNALIKPGYDERLTNEDLAPLRKQTWGTYNIFAFWMSDVHSVGGYI
TAGSLFALGLTSWQVLVSLLVGILIVQYFCNLVAKPSQVTGTPYPVICRASFGVLGANIPAVIRGLIAVAWYGIQTYLAS
SAFLVLALHFFPALAPYADVKLHGFAGLSTLGWAAFMVLWVLQALVFWTGMETIRKFIDWAGPAVYVVMIALAIWLVNKA
GWENVNFTLSFVKYTGWEAVPVMLSAIALVVSYFSGPMLNFGDFSRYAKDFKSVKRGNFWGLPVNFLGFSLLTVITTSAT
LPVFGKLITDPVETVSHIDSTFAMLLGAFTFMTATIGINIVANFVSPAFDFSNVSPKHISWRTGGMIAAVASIFITPWNL
YNNPAVIHYTLDVLGAFIGPLFGILIADYYLVRRQHVDVDDLYTLNPRGRYWYRNGFNPAAVATMIPAAIIPILCVVVPA
WQSAANYSWFIGMGIALVLYRQLAPRMMPGHFGSRAASQA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=481, Percent_Identity=27.8586278586279, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319495, Length=423, Percent_Identity=24.113475177305, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6322219, Length=423, Percent_Identity=23.1678486997636, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6319429, Length=436, Percent_Identity=25.4587155963303, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324766, Length=471, Percent_Identity=21.8683651804671, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6324645, Length=475, Percent_Identity=22.1052631578947, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323266, Length=471, Percent_Identity=22.2929936305732, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 57062; Mature: 56931

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIVKTHICQETQMMQTTATPADAPQVQAGHAAHGNALIKPGYDERLTNEDLAPLRKQT
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCHHCCCCCHHHHHHHC
WGTYNIFAFWMSDVHSVGGYITAGSLFALGLTSWQVLVSLLVGILIVQYFCNLVAKPSQV
CCCHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TGTPYPVICRASFGVLGANIPAVIRGLIAVAWYGIQTYLASSAFLVLALHFFPALAPYAD
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
VKLHGFAGLSTLGWAAFMVLWVLQALVFWTGMETIRKFIDWAGPAVYVVMIALAIWLVNK
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHC
AGWENVNFTLSFVKYTGWEAVPVMLSAIALVVSYFSGPMLNFGDFSRYAKDFKSVKRGNF
CCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
WGLPVNFLGFSLLTVITTSATLPVFGKLITDPVETVSHIDSTFAMLLGAFTFMTATIGIN
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVANFVSPAFDFSNVSPKHISWRTGGMIAAVASIFITPWNLYNNPAVIHYTLDVLGAFIG
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHH
PLFGILIADYYLVRRQHVDVDDLYTLNPRGRYWYRNGFNPAAVATMIPAAIIPILCVVVP
HHHHHHHHHHHHHHHHCCCHHHCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AWQSAANYSWFIGMGIALVLYRQLAPRMMPGHFGSRAASQA
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPIVKTHICQETQMMQTTATPADAPQVQAGHAAHGNALIKPGYDERLTNEDLAPLRKQT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCHHCCCCCHHHHHHHC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HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]