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The map label for this gene is alkB [H]

Identifier: 116696312

GI number: 116696312

Start: 2687531

End: 2688193

Strand: Direct

Name: alkB [H]

Synonym: H16_B2376

Alternate gene names: 116696312

Gene position: 2687531-2688193 (Clockwise)

Preceding gene: 116696311

Following gene: 116696313

Centisome position: 92.28

GC content: 70.14

Gene sequence:

>663_bases
ATGACTTTCGACCTGTTCGACGACCTGCCACCGAACGGCCCCGCCAGCCCCGCCATCGAACCCCTCGCCGACGGCGCCGT
AGTCCTGCGCGGCCTGGCCCGTGCGGATGCCGAAGTGCTGCTGGCCGACGTCCAGGCCATCATCGCGCTTGCGCCCTGGC
GGCACATGATCACCCCCGGCGGGCTGACAATGTCGGTGGCGATGGTCAACTGCGGCACGGTCGGCTGGGTCTCCGATGCG
CGTGGCTATCGCTATGACCCCGTCGATCCGCTGAACGGAAAGCCGTGGCCGGAGATGCCGGCGAGCTTCCGGAAGCTCGC
CACCACTGCTGCGGCGCAAGCGGGTTTTGCCGGTTTCGAACCGGATGCCTGCCTCATCAATCGCTACGAGCCGGGCACCC
GGCTCTCGCTGCACCAGGACCGCGACGAGCGCGATTTCAGCGCGCCCATCGTTTCGGTCTCGCTGGGACTGCCCGCGGTG
TTCCTGTTCGGCGGCATGCGCCGCGCCGACCGCCCGCAGCGGGTGCGGCTGGCGCATGGCGACGTGGTGGTGTGGGGCGG
GCCGTCGCGGCTGGCCTTCCACGGCGTGGCGCCGCTGGCCGACGGCGACCATCCGCTGCTGGGGCGGCTGCGCATCAACC
TGACATTCCGCAAGGCGCTTTAG

Upstream 100 bases:

>100_bases
GACCAAGATCGATACGATCGAGGCTGCATTCCGGGCGCTGGGGAAGCGGCTGGAACTGACGGTTGCCTGACCGCGCCAGG
GCGTCCGTAAACCTCCACCC

Downstream 100 bases:

>100_bases
CCATCACCGCCGCCTTGGCACTTTGCCGGGGGTTTTCTACAGTCAATGGACTGTCCTGAACGCCACCGTGCAAAGGCCCA
CACCATGCATCTCGACGGCT

Product: alkylated DNA repair protein

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTFDLFDDLPPNGPASPAIEPLADGAVVLRGLARADAEVLLADVQAIIALAPWRHMITPGGLTMSVAMVNCGTVGWVSDA
RGYRYDPVDPLNGKPWPEMPASFRKLATTAAAQAGFAGFEPDACLINRYEPGTRLSLHQDRDERDFSAPIVSVSLGLPAV
FLFGGMRRADRPQRVRLAHGDVVVWGGPSRLAFHGVAPLADGDHPLLGRLRINLTFRKAL

Sequences:

>Translated_220_residues
MTFDLFDDLPPNGPASPAIEPLADGAVVLRGLARADAEVLLADVQAIIALAPWRHMITPGGLTMSVAMVNCGTVGWVSDA
RGYRYDPVDPLNGKPWPEMPASFRKLATTAAAQAGFAGFEPDACLINRYEPGTRLSLHQDRDERDFSAPIVSVSLGLPAV
FLFGGMRRADRPQRVRLAHGDVVVWGGPSRLAFHGVAPLADGDHPLLGRLRINLTFRKAL
>Mature_219_residues
TFDLFDDLPPNGPASPAIEPLADGAVVLRGLARADAEVLLADVQAIIALAPWRHMITPGGLTMSVAMVNCGTVGWVSDAR
GYRYDPVDPLNGKPWPEMPASFRKLATTAAAQAGFAGFEPDACLINRYEPGTRLSLHQDRDERDFSAPIVSVSLGLPAVF
LFGGMRRADRPQRVRLAHGDVVVWGGPSRLAFHGVAPLADGDHPLLGRLRINLTFRKAL

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=121, Percent_Identity=33.0578512396694, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1788541, Length=217, Percent_Identity=57.6036866359447, Blast_Score=254, Evalue=4e-69,
Organism=Drosophila melanogaster, GI45555401, Length=98, Percent_Identity=38.7755102040816, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 23670; Mature: 23539

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDLFDDLPPNGPASPAIEPLADGAVVLRGLARADAEVLLADVQAIIALAPWRHMITPG
CCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
GLTMSVAMVNCGTVGWVSDARGYRYDPVDPLNGKPWPEMPASFRKLATTAAAQAGFAGFE
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PDACLINRYEPGTRLSLHQDRDERDFSAPIVSVSLGLPAVFLFGGMRRADRPQRVRLAHG
CCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCCEEEEECC
DVVVWGGPSRLAFHGVAPLADGDHPLLGRLRINLTFRKAL
CEEEECCCCCEEEECCCCCCCCCCCEEEEEEEEEEEEECC
>Mature Secondary Structure 
TFDLFDDLPPNGPASPAIEPLADGAVVLRGLARADAEVLLADVQAIIALAPWRHMITPG
CCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
GLTMSVAMVNCGTVGWVSDARGYRYDPVDPLNGKPWPEMPASFRKLATTAAAQAGFAGFE
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
PDACLINRYEPGTRLSLHQDRDERDFSAPIVSVSLGLPAVFLFGGMRRADRPQRVRLAHG
CCEEEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCHHHHHHCCCCCCCCCCEEEEECC
DVVVWGGPSRLAFHGVAPLADGDHPLLGRLRINLTFRKAL
CEEEECCCCCEEEECCCCCCCCCCCEEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]