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The map label for this gene is pcaQ [H]

Identifier: 116696228

GI number: 116696228

Start: 2591855

End: 2592808

Strand: Direct

Name: pcaQ [H]

Synonym: H16_B2292

Alternate gene names: 116696228

Gene position: 2591855-2592808 (Clockwise)

Preceding gene: 116696223

Following gene: 116696230

Centisome position: 88.99

GC content: 70.23

Gene sequence:

>954_bases
ATGAAACCACAGGCCCCTCCCGCACTCGAAATCTTCCGCAATCGCGTGCGGCTGCGGCACCTCTATTGTTTCGTGGCGGT
GTCGCAGACCCAGCACCTGGGCCGTGCGGCCGAACGCCTGGGCCTGACCCAGCCGGCCGTCTCCAAGACACTGAGCGAGC
TCGAGGAGCTGGCCGGCACCCGCCTGCTGGTGCGGCAGCGGGCCGGCACCGAACTGACCACCGCCGGCACGCGCTTCCTG
CGCCACGCCCTGCGCATCCTCGCGGATATCGATGCCGCGGCGGACAGCATGGCCGGCGCGGATGCGCTGCCGCAGGAGCG
CATCCGGCTGGGTGCGCTGCCGAGCGTGGTGCCGGCCGTGCTGACCGACGCCCTGCTGCGTTTCCGCGCCGCGCATCCCG
AGGTCGGCCTTGCCGTGCAGACCGGCATGAACCGCAACCTCATCGACCAACTCAAGGCGGATGTGCTCGACCTGGTCATT
GGCCGCATGGATGACCCGGTGGCCATGGAGAGCCTGTGGTTCGAGTCCCTGGGCCAGGACTCGCTGGCTCTGGCGGTTCG
CCCCGGGCATCCCCTCGCCGCCGTATCGCGGCCAACGCTGGTGGACGCGCTGGCATGGCCGCTGGTGATTCCGGCCGCGG
GCTCGATTCCCCGCCACAACACGGAAAGCCTGCTCGCCCGGCACGGCCTGCCCCTGCCGGGCGGATGCGTGGAAACCTCG
GATGCCTATCTCGGACGCCTGCTGGCCCGGCAGAGTGACAGCGTGTGGGCAGCGCCGCTCTCCGCAACCAGGCGCGCGGT
GTCCGAGGGAGATCTGGTACTCCTGCCAATCGATACGCAGGGCACCGAGGAACCGATCGGCCTGCTGCGGCACGGCGACC
GCACGCTCACGCCGATGGCCGAGGCGTTGGCAAACTGCATTCGCGCCGCGGCCCAGCCTGCTTCCGCTCAGTGA

Upstream 100 bases:

>100_bases
GGTCATCGGGCAGTCTCCTCGTCGTTGGAATGGTTTGACTTTAAACAACACATAAAGTCATGTAAATTAAGATTACTGTT
AAATACGATAACTTTTAGGC

Downstream 100 bases:

>100_bases
CTTGCGACGCTTGCGCCGCCATTGACTGAGTCGGCGGCACCCGCAGGCCGGCTGCTGAAATGCTTCACCAGCAGTGCACC
GGCGGCAAGGAATGCCGGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKPQAPPALEIFRNRVRLRHLYCFVAVSQTQHLGRAAERLGLTQPAVSKTLSELEELAGTRLLVRQRAGTELTTAGTRFL
RHALRILADIDAAADSMAGADALPQERIRLGALPSVVPAVLTDALLRFRAAHPEVGLAVQTGMNRNLIDQLKADVLDLVI
GRMDDPVAMESLWFESLGQDSLALAVRPGHPLAAVSRPTLVDALAWPLVIPAAGSIPRHNTESLLARHGLPLPGGCVETS
DAYLGRLLARQSDSVWAAPLSATRRAVSEGDLVLLPIDTQGTEEPIGLLRHGDRTLTPMAEALANCIRAAAQPASAQ

Sequences:

>Translated_317_residues
MKPQAPPALEIFRNRVRLRHLYCFVAVSQTQHLGRAAERLGLTQPAVSKTLSELEELAGTRLLVRQRAGTELTTAGTRFL
RHALRILADIDAAADSMAGADALPQERIRLGALPSVVPAVLTDALLRFRAAHPEVGLAVQTGMNRNLIDQLKADVLDLVI
GRMDDPVAMESLWFESLGQDSLALAVRPGHPLAAVSRPTLVDALAWPLVIPAAGSIPRHNTESLLARHGLPLPGGCVETS
DAYLGRLLARQSDSVWAAPLSATRRAVSEGDLVLLPIDTQGTEEPIGLLRHGDRTLTPMAEALANCIRAAAQPASAQ
>Mature_317_residues
MKPQAPPALEIFRNRVRLRHLYCFVAVSQTQHLGRAAERLGLTQPAVSKTLSELEELAGTRLLVRQRAGTELTTAGTRFL
RHALRILADIDAAADSMAGADALPQERIRLGALPSVVPAVLTDALLRFRAAHPEVGLAVQTGMNRNLIDQLKADVLDLVI
GRMDDPVAMESLWFESLGQDSLALAVRPGHPLAAVSRPTLVDALAWPLVIPAAGSIPRHNTESLLARHGLPLPGGCVETS
DAYLGRLLARQSDSVWAAPLSATRRAVSEGDLVLLPIDTQGTEEPIGLLRHGDRTLTPMAEALANCIRAAAQPASAQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081904, Length=300, Percent_Identity=41.3333333333333, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1787601, Length=295, Percent_Identity=27.4576271186441, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787879, Length=222, Percent_Identity=32.8828828828829, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788887, Length=180, Percent_Identity=32.2222222222222, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=170, Percent_Identity=25.2941176470588, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=28.7735849056604, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994962, Length=152, Percent_Identity=32.8947368421053, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787589, Length=220, Percent_Identity=27.7272727272727, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693105, Length=176, Percent_Identity=25, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367136, Length=191, Percent_Identity=30.3664921465969, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=195, Percent_Identity=28.7179487179487, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33924; Mature: 33924

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQAPPALEIFRNRVRLRHLYCFVAVSQTQHLGRAAERLGLTQPAVSKTLSELEELAGT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RLLVRQRAGTELTTAGTRFLRHALRILADIDAAADSMAGADALPQERIRLGALPSVVPAV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHH
LTDALLRFRAAHPEVGLAVQTGMNRNLIDQLKADVLDLVIGRMDDPVAMESLWFESLGQD
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
SLALAVRPGHPLAAVSRPTLVDALAWPLVIPAAGSIPRHNTESLLARHGLPLPGGCVETS
CEEEEECCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCH
DAYLGRLLARQSDSVWAAPLSATRRAVSEGDLVLLPIDTQGTEEPIGLLRHGDRTLTPMA
HHHHHHHHHHCCCCCEECCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHH
EALANCIRAAAQPASAQ
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKPQAPPALEIFRNRVRLRHLYCFVAVSQTQHLGRAAERLGLTQPAVSKTLSELEELAGT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
RLLVRQRAGTELTTAGTRFLRHALRILADIDAAADSMAGADALPQERIRLGALPSVVPAV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHH
LTDALLRFRAAHPEVGLAVQTGMNRNLIDQLKADVLDLVIGRMDDPVAMESLWFESLGQD
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
SLALAVRPGHPLAAVSRPTLVDALAWPLVIPAAGSIPRHNTESLLARHGLPLPGGCVETS
CEEEEECCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCH
DAYLGRLLARQSDSVWAAPLSATRRAVSEGDLVLLPIDTQGTEEPIGLLRHGDRTLTPMA
HHHHHHHHHHCCCCCEECCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHH
EALANCIRAAAQPASAQ
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]