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The map label for this gene is nirF [H]

Identifier: 116696215

GI number: 116696215

Start: 2578765

End: 2580000

Strand: Direct

Name: nirF [H]

Synonym: H16_B2279

Alternate gene names: 116696215

Gene position: 2578765-2580000 (Clockwise)

Preceding gene: 116696214

Following gene: 116696216

Centisome position: 88.54

GC content: 71.52

Gene sequence:

>1236_bases
ATGGCTGATCGATCGACTGCAGGCCGGCAAGCCACCGGCCGCCTCGCCGCAAGGCAACTGATCCGCGGCCTGGCCGCGGC
CTTGTGCGCCACCGTTGCCGCCGCGCTGGTGGCCGGTTGTGCCAGCGCCGCGCGCGGCACCGGCGACCTGGGCGTGGTGG
TGGAGCGCGCCGCCGGACGGCTGCAGATCGTCGAGACCACCGGCATGACCCGGCTGGCAACCGTGGAAGGACTCGGTGAT
TTGTCCCATGCCAGCGTGGTGTTCTCGCGCAACACACGCTACGCCTATGTCTTCGGCCGCGACGGCGGCCTGACGCGCGT
GGACCTGCTCAGCGCCCGCATCACCCACCGCGTGCTGCAGGCGGGCAACAGCATCGGCGGCGCGATCTCGCAGGACGGCC
GCGTGGTGGCCACGCAGAACTACGCGCCCGGCGGCATCCGCCTGTTCGATGCCCAGACGCTTGAGCCGCTGGCTGACCTG
CCGGCCATCGGCAATGACGGCCGCCGCTCCAAGGTGGTGGGCCTGGCCGACCTGCCCGGCCGCCGCTTTGCCGCCAGCCT
GTTCGAATCCGGCGAAATCTGGATCATCGATGCCGGCGATCCGCGTCAGCCGCAGGTGACACGCCTGCCCGCCGGCCGCG
AGCCGTACGACGGACTGGTCACGCCCGATGGCCGCTGGTACCTGGCCGGCCTGTTCGGTGAGGACGGCCTGGCGCTGGTC
GACCTGTGGCAGGCGCCGCTGCAGTCGCGCCGCGTGCTGTCCGGCTACGGCCGCGGCAGCGCGCCGCTGCCGGTCTACAA
GATGCCGCACCTGCGCGGCTGGGCCATGGCCCAGGGCTTGGCCTGGCTGCCGGCGATCGGCCGTCATGAGGTGCTGGTGG
CCGATCCCGCCAACGGCTGGCAGCAGGCCGCGCGCGTGGCGCTGGCCGGCCAGCCGGTCTTTGTGATGGCACGCCCCGAC
GGGCGCCAGGTCTGGGTCAACTTCGCCTTCCCCCACAACGACACGGTGCAGGTGATCGACACCGCCACGCGCCAGGTGGT
GCGCACGCTGCATCCCTGCCGCGGCGTGCTGCACATGGAGTTCACGCCCAAGGGCGAGTCCCTGTGGCTGTCCTGCCGCG
ACGACAACCGTGTCGAGATCTACGACACCGCCAGCCTGGCGCGAATTGCCACGCTGCCCGCCGACAGCCCCAGCGGCATC
TTCTTCACCGTGCGCGCGCAACGGTTCGGCATGTAA

Upstream 100 bases:

>100_bases
CCGGCAAGCCGCCCGAGGGCCTGGTCGCCACCGTGCTGTACGGCCGCCCCGGCACCGCGATGCCGCCCTGGCAACCCTTC
ATGACGCAGGATGAAGCCCA

Downstream 100 bases:

>100_bases
AGGGACACCATGTACGATGAACTGAGCGTCTACGACACCCTGCAGCGCGATTTCCCGCTGCAGCCGCGGCCCTACCAGGC
GCTGGCCGGCAAGCTCGGCC

Product: heme d1 biosynthesis protein (NirF)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MADRSTAGRQATGRLAARQLIRGLAAALCATVAAALVAGCASAARGTGDLGVVVERAAGRLQIVETTGMTRLATVEGLGD
LSHASVVFSRNTRYAYVFGRDGGLTRVDLLSARITHRVLQAGNSIGGAISQDGRVVATQNYAPGGIRLFDAQTLEPLADL
PAIGNDGRRSKVVGLADLPGRRFAASLFESGEIWIIDAGDPRQPQVTRLPAGREPYDGLVTPDGRWYLAGLFGEDGLALV
DLWQAPLQSRRVLSGYGRGSAPLPVYKMPHLRGWAMAQGLAWLPAIGRHEVLVADPANGWQQAARVALAGQPVFVMARPD
GRQVWVNFAFPHNDTVQVIDTATRQVVRTLHPCRGVLHMEFTPKGESLWLSCRDDNRVEIYDTASLARIATLPADSPSGI
FFTVRAQRFGM

Sequences:

>Translated_411_residues
MADRSTAGRQATGRLAARQLIRGLAAALCATVAAALVAGCASAARGTGDLGVVVERAAGRLQIVETTGMTRLATVEGLGD
LSHASVVFSRNTRYAYVFGRDGGLTRVDLLSARITHRVLQAGNSIGGAISQDGRVVATQNYAPGGIRLFDAQTLEPLADL
PAIGNDGRRSKVVGLADLPGRRFAASLFESGEIWIIDAGDPRQPQVTRLPAGREPYDGLVTPDGRWYLAGLFGEDGLALV
DLWQAPLQSRRVLSGYGRGSAPLPVYKMPHLRGWAMAQGLAWLPAIGRHEVLVADPANGWQQAARVALAGQPVFVMARPD
GRQVWVNFAFPHNDTVQVIDTATRQVVRTLHPCRGVLHMEFTPKGESLWLSCRDDNRVEIYDTASLARIATLPADSPSGI
FFTVRAQRFGM
>Mature_410_residues
ADRSTAGRQATGRLAARQLIRGLAAALCATVAAALVAGCASAARGTGDLGVVVERAAGRLQIVETTGMTRLATVEGLGDL
SHASVVFSRNTRYAYVFGRDGGLTRVDLLSARITHRVLQAGNSIGGAISQDGRVVATQNYAPGGIRLFDAQTLEPLADLP
AIGNDGRRSKVVGLADLPGRRFAASLFESGEIWIIDAGDPRQPQVTRLPAGREPYDGLVTPDGRWYLAGLFGEDGLALVD
LWQAPLQSRRVLSGYGRGSAPLPVYKMPHLRGWAMAQGLAWLPAIGRHEVLVADPANGWQQAARVALAGQPVFVMARPDG
RQVWVNFAFPHNDTVQVIDTATRQVVRTLHPCRGVLHMEFTPKGESLWLSCRDDNRVEIYDTASLARIATLPADSPSGIF
FTVRAQRFGM

Specific function: Required for the biosynthesis of heme d1 of nitrite reductase. Could have a dehydrogenase activity yielding sirohydrochlorin from precorrin-2 or dehydrogenation of propionate side chain C17 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011048
- InterPro:   IPR003143
- InterPro:   IPR015943 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 44049; Mature: 43918

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRSTAGRQATGRLAARQLIRGLAAALCATVAAALVAGCASAARGTGDLGVVVERAAGR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCC
LQIVETTGMTRLATVEGLGDLSHASVVFSRNTRYAYVFGRDGGLTRVDLLSARITHRVLQ
EEEEEECCCEEEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHH
AGNSIGGAISQDGRVVATQNYAPGGIRLFDAQTLEPLADLPAIGNDGRRSKVVGLADLPG
CCCCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCEEEEEECCCC
RRFAASLFESGEIWIIDAGDPRQPQVTRLPAGREPYDGLVTPDGRWYLAGLFGEDGLALV
HHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCCCCCEECCCCCEEEEEEECCCCEEEH
DLWQAPLQSRRVLSGYGRGSAPLPVYKMPHLRGWAMAQGLAWLPAIGRHEVLVADPANGW
HHHHCCHHHCHHHHCCCCCCCCCCEEECCCCCCHHHHCCCHHCCCCCCCEEEEECCCCHH
QQAARVALAGQPVFVMARPDGRQVWVNFAFPHNDTVQVIDTATRQVVRTLHPCRGVLHME
HHHHHEEECCCCEEEEECCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCEEEEE
FTPKGESLWLSCRDDNRVEIYDTASLARIATLPADSPSGIFFTVRAQRFGM
ECCCCCEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCEEEEEEEHHCCC
>Mature Secondary Structure 
ADRSTAGRQATGRLAARQLIRGLAAALCATVAAALVAGCASAARGTGDLGVVVERAAGR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCC
LQIVETTGMTRLATVEGLGDLSHASVVFSRNTRYAYVFGRDGGLTRVDLLSARITHRVLQ
EEEEEECCCEEEEEECCCCCCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHH
AGNSIGGAISQDGRVVATQNYAPGGIRLFDAQTLEPLADLPAIGNDGRRSKVVGLADLPG
CCCCCCCCCCCCCEEEEECCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCEEEEEECCCC
RRFAASLFESGEIWIIDAGDPRQPQVTRLPAGREPYDGLVTPDGRWYLAGLFGEDGLALV
HHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCCCCCCEECCCCCEEEEEEECCCCEEEH
DLWQAPLQSRRVLSGYGRGSAPLPVYKMPHLRGWAMAQGLAWLPAIGRHEVLVADPANGW
HHHHCCHHHCHHHHCCCCCCCCCCEEECCCCCCHHHHCCCHHCCCCCCCEEEEECCCCHH
QQAARVALAGQPVFVMARPDGRQVWVNFAFPHNDTVQVIDTATRQVVRTLHPCRGVLHME
HHHHHEEECCCCEEEEECCCCCEEEEEEECCCCCCEEEEHHHHHHHHHHHHHHCCEEEEE
FTPKGESLWLSCRDDNRVEIYDTASLARIATLPADSPSGIFFTVRAQRFGM
ECCCCCEEEEEECCCCEEEEEECCCEEEEEECCCCCCCCEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7588711; 2001732 [H]