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The map label for this gene is narI2 [H]

Identifier: 116696204

GI number: 116696204

Start: 2569090

End: 2569773

Strand: Direct

Name: narI2 [H]

Synonym: H16_B2268

Alternate gene names: 116696204

Gene position: 2569090-2569773 (Clockwise)

Preceding gene: 116696203

Following gene: 116696205

Centisome position: 88.21

GC content: 65.94

Gene sequence:

>684_bases
ATGGCAACCCTCCACCAATTCCTCTATGGCATCTATCCGTACATCGCTGCCGCCATCTTCCTGTTCGGCAGCCTGGCCCG
CTTCGAGCGCGAGCAATACACCTGGAAGACCGACAGCTCGCAGGTGCTGTACCGCGGCAACCTGCGCACCGGCAACATCC
TGTTCCACGTCGGCATCCTCGGCCTGTTCTTCGGCCACCTGGTCGGCCTGCTGACGCCGGTGGCAGTGTGGAATGCGCTG
GGTGTCTCGCACGGCTTCAAGCAGGCCGTGGCAATGGCCGCGGGCGGCGTGATGGGCACCATGTGCCTGGCCGGACTGCT
GATCCTGCTGCACCGCCGCCTGACCAACGCCCGCGTGTCGGCCGTGACCCGCACCGGCGACAAGGTGCTGCTGCTGTGGC
TGCTGGTGACGCTGCTGCTGGGCCTGTCCACGATCTTCGAATCCGCCAGCCACATGGACGGCCACATGATGGTGCAACTG
ATGACCTGGGCCCAGCACCTCGTCACGCTGCGCGGCGACGCCGCCAGCTATATCGCCGATGCGCCGCTGCTGTTCAAGGC
GCACCTGTTCATGGGCATCACGCTGTTCGCGATCTTCCCGTTCACGCGGCTGGTGCATGTGTGGAGCGGCTTCGCCTCGG
TCGGCTATCTCGGCCGCGCCTGGCAACTGGTGCGCAGCCGCTGA

Upstream 100 bases:

>100_bases
ACCCGCGGTGTTCTCCCCTCTCCCGCTTGCGGGAGAGGGGAACACAGTTGGCAATTGAGACGACCGAACCTGCCTCAAAG
GCAGCTACAGAGCAAACACC

Downstream 100 bases:

>100_bases
GCGCCGCCACCCGACAAGGAGAAACAGCATGCCTGTCACCGTCAACGGCGTGGAACTGCGCGACGCCGATATCGAACGCG
AGCTGGACCACCATCACGAT

Product: respiratory nitrate reductase gamma chain (NarI)

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MATLHQFLYGIYPYIAAAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVAVWNAL
GVSHGFKQAVAMAAGGVMGTMCLAGLLILLHRRLTNARVSAVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHLVTLRGDAASYIADAPLLFKAHLFMGITLFAIFPFTRLVHVWSGFASVGYLGRAWQLVRSR

Sequences:

>Translated_227_residues
MATLHQFLYGIYPYIAAAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVAVWNAL
GVSHGFKQAVAMAAGGVMGTMCLAGLLILLHRRLTNARVSAVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQL
MTWAQHLVTLRGDAASYIADAPLLFKAHLFMGITLFAIFPFTRLVHVWSGFASVGYLGRAWQLVRSR
>Mature_226_residues
ATLHQFLYGIYPYIAAAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGILGLFFGHLVGLLTPVAVWNALG
VSHGFKQAVAMAAGGVMGTMCLAGLLILLHRRLTNARVSAVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLM
TWAQHLVTLRGDAASYIADAPLLFKAHLFMGITLFAIFPFTRLVHVWSGFASVGYLGRAWQLVRSR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=46.6960352422907, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1787738, Length=224, Percent_Identity=47.3214285714286, Blast_Score=191, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25127; Mature: 24996

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLHQFLYGIYPYIAAAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
GLFFGHLVGLLTPVAVWNALGVSHGFKQAVAMAAGGVMGTMCLAGLLILLHRRLTNARVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHLVTLRGDAASYIAD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
APLLFKAHLFMGITLFAIFPFTRLVHVWSGFASVGYLGRAWQLVRSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATLHQFLYGIYPYIAAAIFLFGSLARFEREQYTWKTDSSQVLYRGNLRTGNILFHVGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHH
GLFFGHLVGLLTPVAVWNALGVSHGFKQAVAMAAGGVMGTMCLAGLLILLHRRLTNARVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRTGDKVLLLWLLVTLLLGLSTIFESASHMDGHMMVQLMTWAQHLVTLRGDAASYIAD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHC
APLLFKAHLFMGITLFAIFPFTRLVHVWSGFASVGYLGRAWQLVRSR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]