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The map label for this gene is sdhA [C]

Identifier: 116696178

GI number: 116696178

Start: 2541211

End: 2542980

Strand: Direct

Name: sdhA [C]

Synonym: H16_B2242

Alternate gene names: 116696178

Gene position: 2541211-2542980 (Clockwise)

Preceding gene: 116696177

Following gene: 116696179

Centisome position: 87.25

GC content: 67.63

Gene sequence:

>1770_bases
ATGAATACCCTGACTCACGAATACGACATCGTTGTCGTCGGCGGCGGCACCGCCGGCCCGATGGCCGCGATCAAGGCCAA
GGAACGCAACCCCGCGCTGCGCGTGCTGCTGCTCGACAAGGCGCACGTCAAGCGCAGCGGCGCCATCTCGATGGGCATGG
ACGGGCTGAACAATGCCGTGATCCCCGGCCACGCCACGCCCGAGCAGTACACCCGCGAGATCACCGTGGCCAACGACGGC
ATCGTCGACCAGTCCACCGTTTATGCCTACGCGCGCCACAGCTTTGCCACCATCGAGCAGCTGGATCGCTGGGGCGTCAG
GTTCGAGAAGGACGAGACCGGCGACTACGCAGTGAAGAAGGTCCATCACATGGGCTCCTATGTGCTGCCGATGCCGGAAG
GACACAACGTCAAGAAGGTGCTGTACCGCCAGCTCAAGCGCGCGCGGGTAGAGATCACCAACCGCATCGTCGCCACGCGC
GTGCTGACCGACGCCAGCGGCGCCGCCGGCGGCGTGATGGGCTTCGACTGCCGCAGCGCGGACTTCCACGTGATCCGCGC
GCGCGCCGTGATCCTGTGCTGCGGCGCGGCCGGCCGCCTGGGCCTGCCCGCTTCCGGCTACCTGATGGGCACCTACGAGA
ACCCGACCAATGCCGGCGACGGCTATGCCATGGCCTATCACGCCGGCGCCGCGCTGGCCAACCTGGAGTGCTTCCAGATC
AATCCGCTGATCAAGGACTACAACGGCCCGGCCTGCGCCTACGTGACCGGCCCGCTGGGCGGCTATACCGCCAACGGCAA
GGGCGAGCGCTTTATCGAATGCGACTACTGGAGCGGCCAGATGATGTGGGAGTTCTACCAGGAGCTCCAGGGCGGCAACG
GCCCGGTGTTCCTGAAGCTTGACCACCTGGCGGAAGAAACCATCCAGACCATCGAGACGATCCTGCACACCAACGAACGG
CCCAGCCGCGGCCAGTTCCACGCCGGGCGCGGCACCGACTACCGCAGGCAGATGGTGGAGATGCATATCTCGGAGATCGG
CTTCTGCAGCGGCCACAGCGCTTCCGGCGTGCATGTGAACGAGCGCGCCGAGACTACCGTGCCGGGCCTGTACGCGGCCG
GAGACATGGCCGCCGTGCCGCACAACTACATGCTGGGCGCCTTCACCTATGGCTGGTTTGCCGGGCACAACGCGGCCGAC
TACGTCGCCGGCCGCGGCCTGCCTGAAGCCGATGCGGCCCAGGTCGAGGCGGAGCGCGCGCGCGTGTTCGCACCGCTGGC
GCGCGAGAGTGGGCTGCCGCCCGCCCAGGTGGAATACAAGCTGCGCCGCATGGTCAACGACTACCTGCAGCCGCCCAAGG
TCACGCGCAAGATGGAAATCGGCCTGCAGCGTTTTGACGCCATCGCCGAGGACATCGGCCAGATCCGCGCCGCCAATCCG
CACGAGCTGATGCGCGCGGTCGAGGTGATGGCGATCCGCGACTGCGCCGAGATGGCGGCACGCGCCTCCCTGTTCCGCAC
CGAGAGCCGCTGGGGTCTCTACCACCATCGCGTCGACTATCCGCAGCGCAATGATGCGGACTGGTTCTGCCATACGCACC
TGAGCAAGGATGAAGCCGGGCGCATGGTCAGCCACAAGCGCGCGATCGAGCCGTACATCGTCCCGGTGGCAGGCGACGAC
GCCACCGCCTACCAGCGGCTGCGCGTCAGCCGCGGCCAGGCCGCCGCCACGGCCGCCACTGCCGAACCCGCCACCCTGGC
TGCCGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGCAGACCGGCACAAGACCGCGCCAGCCGCCAGCGTCACCAGCCCAGCCGGCCATGCACGGCCCCGCAAACCAGCC
AGCAAGGGGAAGCCCTCCGC

Downstream 100 bases:

>100_bases
AGGAACCCACGCCATGGCCTACACCCCGCATGAGATCGCCCAGCGCAGCAGCGCGCCGGTCACCATCGATGAAGACAAGT
GCATTGCCGACAAGGGCTGC

Product: putative oxidoreductase

Products: FADH2; Fumarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MNTLTHEYDIVVVGGGTAGPMAAIKAKERNPALRVLLLDKAHVKRSGAISMGMDGLNNAVIPGHATPEQYTREITVANDG
IVDQSTVYAYARHSFATIEQLDRWGVRFEKDETGDYAVKKVHHMGSYVLPMPEGHNVKKVLYRQLKRARVEITNRIVATR
VLTDASGAAGGVMGFDCRSADFHVIRARAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGYTANGKGERFIECDYWSGQMMWEFYQELQGGNGPVFLKLDHLAEETIQTIETILHTNER
PSRGQFHAGRGTDYRRQMVEMHISEIGFCSGHSASGVHVNERAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGHNAAD
YVAGRGLPEADAAQVEAERARVFAPLARESGLPPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAIAEDIGQIRAANP
HELMRAVEVMAIRDCAEMAARASLFRTESRWGLYHHRVDYPQRNDADWFCHTHLSKDEAGRMVSHKRAIEPYIVPVAGDD
ATAYQRLRVSRGQAAATAATAEPATLAAA

Sequences:

>Translated_589_residues
MNTLTHEYDIVVVGGGTAGPMAAIKAKERNPALRVLLLDKAHVKRSGAISMGMDGLNNAVIPGHATPEQYTREITVANDG
IVDQSTVYAYARHSFATIEQLDRWGVRFEKDETGDYAVKKVHHMGSYVLPMPEGHNVKKVLYRQLKRARVEITNRIVATR
VLTDASGAAGGVMGFDCRSADFHVIRARAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGYTANGKGERFIECDYWSGQMMWEFYQELQGGNGPVFLKLDHLAEETIQTIETILHTNER
PSRGQFHAGRGTDYRRQMVEMHISEIGFCSGHSASGVHVNERAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGHNAAD
YVAGRGLPEADAAQVEAERARVFAPLARESGLPPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAIAEDIGQIRAANP
HELMRAVEVMAIRDCAEMAARASLFRTESRWGLYHHRVDYPQRNDADWFCHTHLSKDEAGRMVSHKRAIEPYIVPVAGDD
ATAYQRLRVSRGQAAATAATAEPATLAAA
>Mature_589_residues
MNTLTHEYDIVVVGGGTAGPMAAIKAKERNPALRVLLLDKAHVKRSGAISMGMDGLNNAVIPGHATPEQYTREITVANDG
IVDQSTVYAYARHSFATIEQLDRWGVRFEKDETGDYAVKKVHHMGSYVLPMPEGHNVKKVLYRQLKRARVEITNRIVATR
VLTDASGAAGGVMGFDCRSADFHVIRARAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
NPLIKDYNGPACAYVTGPLGGYTANGKGERFIECDYWSGQMMWEFYQELQGGNGPVFLKLDHLAEETIQTIETILHTNER
PSRGQFHAGRGTDYRRQMVEMHISEIGFCSGHSASGVHVNERAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGHNAAD
YVAGRGLPEADAAQVEAERARVFAPLARESGLPPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAIAEDIGQIRAANP
HELMRAVEVMAIRDCAEMAARASLFRTESRWGLYHHRVDYPQRNDADWFCHTHLSKDEAGRMVSHKRAIEPYIVPVAGDD
ATAYQRLRVSRGQAAATAATAEPATLAAA

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786942, Length=457, Percent_Identity=26.9146608315098, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1790597, Length=587, Percent_Identity=21.9761499148211, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=578, Percent_Identity=23.8754325259516, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1 [C]

Molecular weight: Translated: 64533; Mature: 64533

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLTHEYDIVVVGGGTAGPMAAIKAKERNPALRVLLLDKAHVKRSGAISMGMDGLNNAV
CCCCCEEEEEEEECCCCCCCHHHEEECCCCCEEEEEEECCHHHCCCCCEEECCCCCCCCC
IPGHATPEQYTREITVANDGIVDQSTVYAYARHSFATIEQLDRWGVRFEKDETGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHNVKKVLYRQLKRARVEITNRIVATRVLTDASGAAGGVMGFDCRSA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEECCCCCC
DFHVIRARAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
CCEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHCCEEEEE
NPLIKDYNGPACAYVTGPLGGYTANGKGERFIECDYWSGQMMWEFYQELQGGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
DHLAEETIQTIETILHTNERPSRGQFHAGRGTDYRRQMVEMHISEIGFCSGHSASGVHVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEC
ERAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGHNAADYVAGRGLPEADAAQVEAERA
CCCCCCCCCEEECCCCCCCCCCCEEHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHH
RVFAPLARESGLPPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAIAEDIGQIRAANP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
HELMRAVEVMAIRDCAEMAARASLFRTESRWGLYHHRVDYPQRNDADWFCHTHLSKDEAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHH
RMVSHKRAIEPYIVPVAGDDATAYQRLRVSRGQAAATAATAEPATLAAA
HHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNTLTHEYDIVVVGGGTAGPMAAIKAKERNPALRVLLLDKAHVKRSGAISMGMDGLNNAV
CCCCCEEEEEEEECCCCCCCHHHEEECCCCCEEEEEEECCHHHCCCCCEEECCCCCCCCC
IPGHATPEQYTREITVANDGIVDQSTVYAYARHSFATIEQLDRWGVRFEKDETGDYAVKK
CCCCCCHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHH
VHHMGSYVLPMPEGHNVKKVLYRQLKRARVEITNRIVATRVLTDASGAAGGVMGFDCRSA
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEECCCCCC
DFHVIRARAVILCCGAAGRLGLPASGYLMGTYENPTNAGDGYAMAYHAGAALANLECFQI
CCEEEEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCHHHCCEEEEE
NPLIKDYNGPACAYVTGPLGGYTANGKGERFIECDYWSGQMMWEFYQELQGGNGPVFLKL
CCHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
DHLAEETIQTIETILHTNERPSRGQFHAGRGTDYRRQMVEMHISEIGFCSGHSASGVHVN
HHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEC
ERAETTVPGLYAAGDMAAVPHNYMLGAFTYGWFAGHNAADYVAGRGLPEADAAQVEAERA
CCCCCCCCCEEECCCCCCCCCCCEEHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHH
RVFAPLARESGLPPAQVEYKLRRMVNDYLQPPKVTRKMEIGLQRFDAIAEDIGQIRAANP
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
HELMRAVEVMAIRDCAEMAARASLFRTESRWGLYHHRVDYPQRNDADWFCHTHLSKDEAG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCCHHHH
RMVSHKRAIEPYIVPVAGDDATAYQRLRVSRGQAAATAATAEPATLAAA
HHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: FAD; Succinate [C]

Specific reaction: FAD + Succinate --> FADH2 + Fumarate [C]

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]