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The map label for this gene is mrcA [H]

Identifier: 116696084

GI number: 116696084

Start: 2428714

End: 2431182

Strand: Direct

Name: mrcA [H]

Synonym: H16_B2148

Alternate gene names: 116696084

Gene position: 2428714-2431182 (Clockwise)

Preceding gene: 116696082

Following gene: 116696087

Centisome position: 83.39

GC content: 70.19

Gene sequence:

>2469_bases
ATGAGGCTGGCTGAGATCAAGACCCGGCTTGCCCCGCTGCTCGCCTCGCTGCAGGCTTCATTGCCTGCCCCCCTCCAGCA
ACGCCTGTCAGGCATGGCCGCGCGCCTTGCACGCGCCGGCCTGACCCGGCCCGGGTCGCTGCGCCCCACGCGGCGCGGCG
TGCTGGTGGGCCTGGCCGCCATCCCGGCGGCCCTCCTGCTCTATGTGCTGGTCCTGATCCCGTTCACGCCGGGCATCAGC
GATATCCGCAAGGCCAAGTCCGAGCAGCCGGCCCAGGTGCTGTCCGCCGACGGCAAGGAGCTGGCGGTGTTCAAGTGGGC
CAACCGCGACTGGGTGCCGCTGTCGCAGATTTCACCCAATGTGGTGGCTGCGCTGATCTCCACCGAAGACCACCGCTTCT
ACCACCACCACGGGCTGGATTGGCGCAGGCTGGCCTCGGCCGCGGTGCATACCTTCTCCGGCGACCGCCAGGGCGGGTCC
ACCATCACCCAGCAGCTGGCCCGCAACCTCTATCCCGATGAAATTGGCCGCGCGCCCACGCTGACGCGCAAGCTCAAGGA
AGCCATCACCGCGCTGAAGATTGAGGCGCTCTATACCAAGGACGAGATCCTCGAGACCTATCTCAACACCGTGCCGTTCC
TGTACAACGCGTTCGGCATCGAGATGGCGGCGCGCACCTACTTCGACCGCCCGGCGCGCTCGCTCGACGTGCTGCAGAGC
GCCACGCTGATCGGCATGCTCAAGGGCAACAGCTACTACAACCCGGTGCTCAACCCCGAGCGCGCGCTCGACCGGCGCAA
TATCGTGCTGGGCCAGATGGTCAAGCGCGGCAAGCTGGATGCGGCGCGCTACGAGCAGCTGAAGAAGCGCCCGCTGCGCA
TCGACTTCGAGCGCCAGACCGAGCCGCCAGGCCCCGCGCCACATTTCGCGCAGCAGCTGCGCAAGTGGCTGATCGGCTGG
GCCGACCGCAATGGCTACGACATCTACGCCGACGGCCTGGTGGTGCATACCACCATCGACGCCCGCCTGCAGGCCATGGC
CACGCAGGCCGTGGTGCAGCAGGGCAACCGGCTGCAGGCCATCGCCAACGCGGCCTGGGCGCCGCGCGCGGGCTGGGCCG
CCAACCTGCCGCTGGTGCAGGCCTTCGTGCGCGAAACCCCGGAGTACCGTGCCGCCATCGCCGCCGGCACCGCGCCGGAA
GAGGCGCTGCGCCATCTGATGTCCGACCATGCCTTCATGCGCGCGCTGCACCAGCAGAAGACGCGCATCCAGGCGGGCTT
CATGGCCATCGATCCGCGCAACGGCGAGATCCGCGCCTGGGTCGGCAGCCGCGACTACACCCAGGACGCGTTCGACCACG
TGCAGCAGGCGCGCCGCCAGCCGGGCTCGACCTTCAAGCCCTTTGTCTACGGCGCGGCCTTTGAAGAAGGCAAGACCCCC
GACGACACGCTGGTGGACCAGCCGGTGGAAATCGAACTGGCTGGCGGCGAGGTCTGGCGCCCCACCGATGAAGGCCGCCC
CACCGGCCGCGCCATGACGCTGCGCGACGGCCTGGTCTATTCGCGCAACACCATCACCGCGCAGCTGATGCACGAAGTGG
GGCCGACGCGCGTGGCGCGGCTGGCGCGCGACATGGGCGTGCGCGACAGCCAGCTCGACGTGGTGCCGTCGCTGGCGCTG
GGCACCAGCCCGGTCACGCTCAAGGAGATGGTGGCCGCCTACGGCACCATCGCCAACGCCGGCGACTACATCGCGCCCAC
CATGGTGACGCGCATTGAAGACCGCCAGGGCAATGTGCTGCAGGTGTTCCGGCCGGCGCGCGCCGAGCACGCGCTGCCCG
CCGCCACCACGCAAACCCTGCTCGACGTGATGCGCGACGTGATCGACCGCGGCACCGGCGCCAGCATCCGCTCGCGCTTC
GGCATCCGCGCCGACGTGGCCGGCAAGACCGGCACCACCCAGGACAATGCCGACGGCTGGTTCATCCTGATGCATCCGGA
ACTGGTGGCGGGCGCCTGGGTCGGCTTCAACGACAGCCGCGTCACGCTGCGCAGCGACTACTGGGGCCAGGGCGCGCACA
GCGCGCTGCCGATGGTGGGCGACTTCTACCAGCGCGCGCTGCGCTCGCGCATCGTCGACGGCCGCGCGCGCTTTGCCGAG
CACGAAGAGACCAGCCTGTTCGCATCGCTGGGAACGCAGCTGCGCGGCTGGTTCAGCCAGCTGTTCACGCGCGACAAGGC
CAGCGAGACCCCGGCGCCGCGCCCGGCACCGCGTCGGCCGGCGCTGCCCACGCCGGAGGCCGAGGTCGCGCCGCCGGCCG
GCGCGGCATCGTCTGCCGCGGTGGATGTCGGCCCGGATGAGTCCAGCCTGGCGCTGGATTCGGGGGGAGACGGTGATTGC
GGTGCCGCAGGACATGAGCGCGGGCAGCGCTCCGGTTGCGCCACCCCCTCAGGCCGAACCAGCGCCTAA

Upstream 100 bases:

>100_bases
GTAACCAAGGGGAACGTTTTGGTACGATGCATGCTTTTCCGACGACGGTAATGCCAGTGCCCCGTGCCGCCATGTGCCTG
TGCCTCGACCGCTTCGCCTC

Downstream 100 bases:

>100_bases
CGCCGCCCCTGCCTCCCCGGCTTCCGCAAATCCGCCCGCACCGCCTGCGCCCCCAGCGCCCATCACGCCCCTCGCGCCAA
CCATCATCACCCCAGCCAAC

Product: membrane carboxypeptidase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 822; Mature: 822

Protein sequence:

>822_residues
MRLAEIKTRLAPLLASLQASLPAPLQQRLSGMAARLARAGLTRPGSLRPTRRGVLVGLAAIPAALLLYVLVLIPFTPGIS
DIRKAKSEQPAQVLSADGKELAVFKWANRDWVPLSQISPNVVAALISTEDHRFYHHHGLDWRRLASAAVHTFSGDRQGGS
TITQQLARNLYPDEIGRAPTLTRKLKEAITALKIEALYTKDEILETYLNTVPFLYNAFGIEMAARTYFDRPARSLDVLQS
ATLIGMLKGNSYYNPVLNPERALDRRNIVLGQMVKRGKLDAARYEQLKKRPLRIDFERQTEPPGPAPHFAQQLRKWLIGW
ADRNGYDIYADGLVVHTTIDARLQAMATQAVVQQGNRLQAIANAAWAPRAGWAANLPLVQAFVRETPEYRAAIAAGTAPE
EALRHLMSDHAFMRALHQQKTRIQAGFMAIDPRNGEIRAWVGSRDYTQDAFDHVQQARRQPGSTFKPFVYGAAFEEGKTP
DDTLVDQPVEIELAGGEVWRPTDEGRPTGRAMTLRDGLVYSRNTITAQLMHEVGPTRVARLARDMGVRDSQLDVVPSLAL
GTSPVTLKEMVAAYGTIANAGDYIAPTMVTRIEDRQGNVLQVFRPARAEHALPAATTQTLLDVMRDVIDRGTGASIRSRF
GIRADVAGKTGTTQDNADGWFILMHPELVAGAWVGFNDSRVTLRSDYWGQGAHSALPMVGDFYQRALRSRIVDGRARFAE
HEETSLFASLGTQLRGWFSQLFTRDKASETPAPRPAPRRPALPTPEAEVAPPAGAASSAAVDVGPDESSLALDSGGDGDC
GAAGHERGQRSGCATPSGRTSA

Sequences:

>Translated_822_residues
MRLAEIKTRLAPLLASLQASLPAPLQQRLSGMAARLARAGLTRPGSLRPTRRGVLVGLAAIPAALLLYVLVLIPFTPGIS
DIRKAKSEQPAQVLSADGKELAVFKWANRDWVPLSQISPNVVAALISTEDHRFYHHHGLDWRRLASAAVHTFSGDRQGGS
TITQQLARNLYPDEIGRAPTLTRKLKEAITALKIEALYTKDEILETYLNTVPFLYNAFGIEMAARTYFDRPARSLDVLQS
ATLIGMLKGNSYYNPVLNPERALDRRNIVLGQMVKRGKLDAARYEQLKKRPLRIDFERQTEPPGPAPHFAQQLRKWLIGW
ADRNGYDIYADGLVVHTTIDARLQAMATQAVVQQGNRLQAIANAAWAPRAGWAANLPLVQAFVRETPEYRAAIAAGTAPE
EALRHLMSDHAFMRALHQQKTRIQAGFMAIDPRNGEIRAWVGSRDYTQDAFDHVQQARRQPGSTFKPFVYGAAFEEGKTP
DDTLVDQPVEIELAGGEVWRPTDEGRPTGRAMTLRDGLVYSRNTITAQLMHEVGPTRVARLARDMGVRDSQLDVVPSLAL
GTSPVTLKEMVAAYGTIANAGDYIAPTMVTRIEDRQGNVLQVFRPARAEHALPAATTQTLLDVMRDVIDRGTGASIRSRF
GIRADVAGKTGTTQDNADGWFILMHPELVAGAWVGFNDSRVTLRSDYWGQGAHSALPMVGDFYQRALRSRIVDGRARFAE
HEETSLFASLGTQLRGWFSQLFTRDKASETPAPRPAPRRPALPTPEAEVAPPAGAASSAAVDVGPDESSLALDSGGDGDC
GAAGHERGQRSGCATPSGRTSA
>Mature_822_residues
MRLAEIKTRLAPLLASLQASLPAPLQQRLSGMAARLARAGLTRPGSLRPTRRGVLVGLAAIPAALLLYVLVLIPFTPGIS
DIRKAKSEQPAQVLSADGKELAVFKWANRDWVPLSQISPNVVAALISTEDHRFYHHHGLDWRRLASAAVHTFSGDRQGGS
TITQQLARNLYPDEIGRAPTLTRKLKEAITALKIEALYTKDEILETYLNTVPFLYNAFGIEMAARTYFDRPARSLDVLQS
ATLIGMLKGNSYYNPVLNPERALDRRNIVLGQMVKRGKLDAARYEQLKKRPLRIDFERQTEPPGPAPHFAQQLRKWLIGW
ADRNGYDIYADGLVVHTTIDARLQAMATQAVVQQGNRLQAIANAAWAPRAGWAANLPLVQAFVRETPEYRAAIAAGTAPE
EALRHLMSDHAFMRALHQQKTRIQAGFMAIDPRNGEIRAWVGSRDYTQDAFDHVQQARRQPGSTFKPFVYGAAFEEGKTP
DDTLVDQPVEIELAGGEVWRPTDEGRPTGRAMTLRDGLVYSRNTITAQLMHEVGPTRVARLARDMGVRDSQLDVVPSLAL
GTSPVTLKEMVAAYGTIANAGDYIAPTMVTRIEDRQGNVLQVFRPARAEHALPAATTQTLLDVMRDVIDRGTGASIRSRF
GIRADVAGKTGTTQDNADGWFILMHPELVAGAWVGFNDSRVTLRSDYWGQGAHSALPMVGDFYQRALRSRIVDGRARFAE
HEETSLFASLGTQLRGWFSQLFTRDKASETPAPRPAPRRPALPTPEAEVAPPAGAASSAAVDVGPDESSLALDSGGDGDC
GAAGHERGQRSGCATPSGRTSA

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=588, Percent_Identity=31.1224489795918, Blast_Score=257, Evalue=2e-69,
Organism=Escherichia coli, GI1788867, Length=665, Percent_Identity=26.7669172932331, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1786343, Length=255, Percent_Identity=35.6862745098039, Blast_Score=122, Evalue=8e-29,
Organism=Escherichia coli, GI1789601, Length=174, Percent_Identity=36.7816091954023, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 89885; Mature: 89885

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLAEIKTRLAPLLASLQASLPAPLQQRLSGMAARLARAGLTRPGSLRPTRRGVLVGLAA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IPAALLLYVLVLIPFTPGISDIRKAKSEQPAQVLSADGKELAVFKWANRDWVPLSQISPN
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHCCHH
VVAALISTEDHRFYHHHGLDWRRLASAAVHTFSGDRQGGSTITQQLARNLYPDEIGRAPT
HHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCH
LTRKLKEAITALKIEALYTKDEILETYLNTVPFLYNAFGIEMAARTYFDRPARSLDVLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ATLIGMLKGNSYYNPVLNPERALDRRNIVLGQMVKRGKLDAARYEQLKKRPLRIDFERQT
HHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
EPPGPAPHFAQQLRKWLIGWADRNGYDIYADGLVVHTTIDARLQAMATQAVVQQGNRLQA
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHCCCHHHH
IANAAWAPRAGWAANLPLVQAFVRETPEYRAAIAAGTAPEEALRHLMSDHAFMRALHQQK
HHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TRIQAGFMAIDPRNGEIRAWVGSRDYTQDAFDHVQQARRQPGSTFKPFVYGAAFEEGKTP
HHHHHCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHCCCCC
DDTLVDQPVEIELAGGEVWRPTDEGRPTGRAMTLRDGLVYSRNTITAQLMHEVGPTRVAR
CHHHCCCCEEEEECCCCEECCCCCCCCCCCEEEEECCEEECCCHHHHHHHHHCCHHHHHH
LARDMGVRDSQLDVVPSLALGTSPVTLKEMVAAYGTIANAGDYIAPTMVTRIEDRQGNVL
HHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEE
QVFRPARAEHALPAATTQTLLDVMRDVIDRGTGASIRSRFGIRADVAGKTGTTQDNADGW
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCCCCCCCCEE
FILMHPELVAGAWVGFNDSRVTLRSDYWGQGAHSALPMVGDFYQRALRSRIVDGRARFAE
EEEECCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
HEETSLFASLGTQLRGWFSQLFTRDKASETPAPRPAPRRPALPTPEAEVAPPAGAASSAA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VDVGPDESSLALDSGGDGDCGAAGHERGQRSGCATPSGRTSA
EECCCCCCCEEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLAEIKTRLAPLLASLQASLPAPLQQRLSGMAARLARAGLTRPGSLRPTRRGVLVGLAA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
IPAALLLYVLVLIPFTPGISDIRKAKSEQPAQVLSADGKELAVFKWANRDWVPLSQISPN
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHCCHH
VVAALISTEDHRFYHHHGLDWRRLASAAVHTFSGDRQGGSTITQQLARNLYPDEIGRAPT
HHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHCCCCH
LTRKLKEAITALKIEALYTKDEILETYLNTVPFLYNAFGIEMAARTYFDRPARSLDVLQS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ATLIGMLKGNSYYNPVLNPERALDRRNIVLGQMVKRGKLDAARYEQLKKRPLRIDFERQT
HHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
EPPGPAPHFAQQLRKWLIGWADRNGYDIYADGLVVHTTIDARLQAMATQAVVQQGNRLQA
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHCCCHHHH
IANAAWAPRAGWAANLPLVQAFVRETPEYRAAIAAGTAPEEALRHLMSDHAFMRALHQQK
HHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
TRIQAGFMAIDPRNGEIRAWVGSRDYTQDAFDHVQQARRQPGSTFKPFVYGAAFEEGKTP
HHHHHCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEECHHHCCCCC
DDTLVDQPVEIELAGGEVWRPTDEGRPTGRAMTLRDGLVYSRNTITAQLMHEVGPTRVAR
CHHHCCCCEEEEECCCCEECCCCCCCCCCCEEEEECCEEECCCHHHHHHHHHCCHHHHHH
LARDMGVRDSQLDVVPSLALGTSPVTLKEMVAAYGTIANAGDYIAPTMVTRIEDRQGNVL
HHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEE
QVFRPARAEHALPAATTQTLLDVMRDVIDRGTGASIRSRFGIRADVAGKTGTTQDNADGW
EEECCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCCCCCCCCEE
FILMHPELVAGAWVGFNDSRVTLRSDYWGQGAHSALPMVGDFYQRALRSRIVDGRARFAE
EEEECCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
HEETSLFASLGTQLRGWFSQLFTRDKASETPAPRPAPRRPALPTPEAEVAPPAGAASSAA
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VDVGPDESSLALDSGGDGDCGAAGHERGQRSGCATPSGRTSA
EECCCCCCCEEECCCCCCCCCCCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]