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The map label for this gene is fusA [H]

Identifier: 116695847

GI number: 116695847

Start: 2157403

End: 2159457

Strand: Direct

Name: fusA [H]

Synonym: H16_B1911

Alternate gene names: 116695847

Gene position: 2157403-2159457 (Clockwise)

Preceding gene: 116695846

Following gene: 116695852

Centisome position: 74.07

GC content: 66.42

Gene sequence:

>2055_bases
ATGCACGATAGTCCCGATGCCATCCGCACCGTCGCCTTGCTAGGGCACGCGGGGTGCGGCAAGACCTCGCTGGTCGAAGC
GCTGTTGCACAAGGGCGGTGCCCTGCACACGCCCGGCAGCGTCGAACGCGGTTCCACGGTGTGCGACAGCGACCCGCTCG
AGCGCAAGTACAAACGCTCGCTCACCACCGCCGCCGCCCACCTGCACTACCGCGACACCCGCATCTACCTGCTTGACACC
CCCGGTTATCCCGACTTCTCCGGCCTCGCCATCAGCGCGCTGGCGGCGGTGGAAACCGCCGCCATCGTCATCAATGCCCA
GACCGGCATCGAGATGACCACGCGCCGCGCGATGGCGTGGGCGCAGGCGCGCCAGCTGTGCCGGATGATCATCGTCAACG
GCATCGACGGCGACAAGGTCGACCTGCCCGCGCTGCTTGCGGACATCCAGGAGGCCTTTGGCAAGGAATGCCTGCCGATC
AACCTGCCTGCCGGCAACGGCAGCCAGGTGGTGGACTGCTTCTTCAATCCGGCCGGCGAGTCGGATTTTTTATCTGTTCC
CGCCGCCCACGATGCGCTGATCGACCAGGTGATCGAGATCGATCCCGAACTGATGGAGCTGTACCTGGAACAAGGCGAGG
CGATCACGCCGGAGCAGCTGCACGCGCCATTCGAGCGCGCGCTGCGCGAAGGCCACCTGGTGCCGATCTGCTTTACCTCG
GCCGCCACCGGCGCCGGCATCACGGAACTGCTCGACGTGTTCGTGCAACTGCTGCCCAACCCGACTGAAGGCAATCCGCC
GCTGTTCTATCGCGATACGGGCGATGGCAGCGAACACCGCAAGGCCGTGCGCGCCGAGCCCGTGCCTGACAAGCACGTGC
TGGCCCATGTCTTCAAGGTGGTGATCGACCCCTATGTCGGCAAGCTGGCGGTGTTCCGCATCCACCAGGGCACGGTCACG
CGCGACAGCCAGCTCTATATCGGCGACGGGCGCCAGCCGTTCAAGGTGGCGCACCTGCTGCGGCTGCAGGGCAAGGAGAC
CCAGGAGATCCCGCGGGCCGGCCCGGGCGATATCTGCGCAGTGGCCAAGGTCGACGAACTGGGCTTCGATGCCGTGCTGC
ACGACGCCACCGAGGACGGCAATATCCACCTGACGCCGCTGGAGTTCCCAACTCCCATCTACGGGCTGGCGATCGAACCG
GCGCGGCGCGGCAACGAGCAGCGCCTGGCCGAAGTGCTGCACAAGCTTAGCGCGGAAGACCCGTGCCTGCGCGTCGAGCA
TCCGGTTGGCACCAATGAGACCGTGGTGTTCGGCCTGGGCGAATTCCATCTGCGCTGCGCGCTGGAGCGGCTGACCGAGC
AATACAAGCTGGAGGTGGTGACGCGCCCGCCCAAGATTGCCTATCGCGAAACCATCGGCGGCAAGGCCGAGGGCCATCAC
CGGCACAAGAAGCAGACCGGCGGCGCCGGGCAGTTCGGTGAAGTGATGCTGCGCGTGGAGCCGCTGGCGCGCGGCGAGGG
CTTCGACTTTGTCGATGCGGTCAAGGGCGGCGCGATTCCCGGCCAGTTCATTCCCGCCGTGGAAAAAGGCATCCGCCAGG
TGCTGGACAGCGGCCCGCTGGCGGGCTTCCCGATGCAGGACGTGCGCGTGACCGTGTATGACGGCAAGAGCCATCCGGTC
GATTCCAAGGAAGTCGCGTTCGCGACCGCCGGGCGCAAGGCCTTTATCGACGCGGTGCTCAAGGCCAGGCCCAGCGTGCT
GGAACCGATCGTCGACATCGAGGTGACGGCGCCGGGCACAGCCATGGGCGACATCATCGGCGACCTCTCAACCAAGCGCG
GCCAGGTCCACGGCACGCGCACCGCGGTCGGCAACGCCGTGGTGGTGGCCGGCCAGGTGCCGCTGTCCGAGCTCAACGAC
TACCAGTCACGGCTGAACAGCATCACCGGCGGCCACGGCAGCTATACCATCCAGTTCAGCCACTACGACAACGTGCCGCC
GGCGCAGCAGGACAAGATGGCATCGAAACACAAGGCGCAGCAGGATACGGATTGA

Upstream 100 bases:

>100_bases
TCTTGCGTCAGGCAATGCACCGAGTCGGGCATCCGCGTATGTCGCTTCCGGCGAATTTCGCCAATAATTCCCGGACAGCC
ATTGCCGGACGAGAGCCACC

Downstream 100 bases:

>100_bases
GGGGTTCGCGCCCGCGGCTGCATCTAGTACGTTTCCAGATGCAGCCGCCCTTCCCGCTTCAACCCTTCAGCCAGCGCCTC
CCATTGCAGCCCCGCCTGCT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MHDSPDAIRTVALLGHAGCGKTSLVEALLHKGGALHTPGSVERGSTVCDSDPLERKYKRSLTTAAAHLHYRDTRIYLLDT
PGYPDFSGLAISALAAVETAAIVINAQTGIEMTTRRAMAWAQARQLCRMIIVNGIDGDKVDLPALLADIQEAFGKECLPI
NLPAGNGSQVVDCFFNPAGESDFLSVPAAHDALIDQVIEIDPELMELYLEQGEAITPEQLHAPFERALREGHLVPICFTS
AATGAGITELLDVFVQLLPNPTEGNPPLFYRDTGDGSEHRKAVRAEPVPDKHVLAHVFKVVIDPYVGKLAVFRIHQGTVT
RDSQLYIGDGRQPFKVAHLLRLQGKETQEIPRAGPGDICAVAKVDELGFDAVLHDATEDGNIHLTPLEFPTPIYGLAIEP
ARRGNEQRLAEVLHKLSAEDPCLRVEHPVGTNETVVFGLGEFHLRCALERLTEQYKLEVVTRPPKIAYRETIGGKAEGHH
RHKKQTGGAGQFGEVMLRVEPLARGEGFDFVDAVKGGAIPGQFIPAVEKGIRQVLDSGPLAGFPMQDVRVTVYDGKSHPV
DSKEVAFATAGRKAFIDAVLKARPSVLEPIVDIEVTAPGTAMGDIIGDLSTKRGQVHGTRTAVGNAVVVAGQVPLSELND
YQSRLNSITGGHGSYTIQFSHYDNVPPAQQDKMASKHKAQQDTD

Sequences:

>Translated_684_residues
MHDSPDAIRTVALLGHAGCGKTSLVEALLHKGGALHTPGSVERGSTVCDSDPLERKYKRSLTTAAAHLHYRDTRIYLLDT
PGYPDFSGLAISALAAVETAAIVINAQTGIEMTTRRAMAWAQARQLCRMIIVNGIDGDKVDLPALLADIQEAFGKECLPI
NLPAGNGSQVVDCFFNPAGESDFLSVPAAHDALIDQVIEIDPELMELYLEQGEAITPEQLHAPFERALREGHLVPICFTS
AATGAGITELLDVFVQLLPNPTEGNPPLFYRDTGDGSEHRKAVRAEPVPDKHVLAHVFKVVIDPYVGKLAVFRIHQGTVT
RDSQLYIGDGRQPFKVAHLLRLQGKETQEIPRAGPGDICAVAKVDELGFDAVLHDATEDGNIHLTPLEFPTPIYGLAIEP
ARRGNEQRLAEVLHKLSAEDPCLRVEHPVGTNETVVFGLGEFHLRCALERLTEQYKLEVVTRPPKIAYRETIGGKAEGHH
RHKKQTGGAGQFGEVMLRVEPLARGEGFDFVDAVKGGAIPGQFIPAVEKGIRQVLDSGPLAGFPMQDVRVTVYDGKSHPV
DSKEVAFATAGRKAFIDAVLKARPSVLEPIVDIEVTAPGTAMGDIIGDLSTKRGQVHGTRTAVGNAVVVAGQVPLSELND
YQSRLNSITGGHGSYTIQFSHYDNVPPAQQDKMASKHKAQQDTD
>Mature_684_residues
MHDSPDAIRTVALLGHAGCGKTSLVEALLHKGGALHTPGSVERGSTVCDSDPLERKYKRSLTTAAAHLHYRDTRIYLLDT
PGYPDFSGLAISALAAVETAAIVINAQTGIEMTTRRAMAWAQARQLCRMIIVNGIDGDKVDLPALLADIQEAFGKECLPI
NLPAGNGSQVVDCFFNPAGESDFLSVPAAHDALIDQVIEIDPELMELYLEQGEAITPEQLHAPFERALREGHLVPICFTS
AATGAGITELLDVFVQLLPNPTEGNPPLFYRDTGDGSEHRKAVRAEPVPDKHVLAHVFKVVIDPYVGKLAVFRIHQGTVT
RDSQLYIGDGRQPFKVAHLLRLQGKETQEIPRAGPGDICAVAKVDELGFDAVLHDATEDGNIHLTPLEFPTPIYGLAIEP
ARRGNEQRLAEVLHKLSAEDPCLRVEHPVGTNETVVFGLGEFHLRCALERLTEQYKLEVVTRPPKIAYRETIGGKAEGHH
RHKKQTGGAGQFGEVMLRVEPLARGEGFDFVDAVKGGAIPGQFIPAVEKGIRQVLDSGPLAGFPMQDVRVTVYDGKSHPV
DSKEVAFATAGRKAFIDAVLKARPSVLEPIVDIEVTAPGTAMGDIIGDLSTKRGQVHGTRTAVGNAVVVAGQVPLSELND
YQSRLNSITGGHGSYTIQFSHYDNVPPAQQDKMASKHKAQQDTD

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=697, Percent_Identity=27.8335724533716, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI19923640, Length=727, Percent_Identity=25.8596973865199, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI25306287, Length=305, Percent_Identity=28.5245901639344, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI25306283, Length=382, Percent_Identity=26.1780104712042, Blast_Score=100, Evalue=8e-21,
Organism=Homo sapiens, GI4503483, Length=437, Percent_Identity=22.6544622425629, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1789738, Length=693, Percent_Identity=31.1688311688312, Blast_Score=339, Evalue=4e-94,
Organism=Escherichia coli, GI1790835, Length=481, Percent_Identity=25.1559251559252, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI48994988, Length=200, Percent_Identity=26, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17533571, Length=707, Percent_Identity=28.4299858557284, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17556745, Length=716, Percent_Identity=22.7653631284916, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17506493, Length=437, Percent_Identity=22.1967963386728, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=706, Percent_Identity=27.7620396600567, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6322359, Length=775, Percent_Identity=22.7096774193548, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24582462, Length=707, Percent_Identity=28.005657708628, Blast_Score=280, Evalue=4e-75,
Organism=Drosophila melanogaster, GI221458488, Length=731, Percent_Identity=25.4445964432285, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 73898; Mature: 73898

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDSPDAIRTVALLGHAGCGKTSLVEALLHKGGALHTPGSVERGSTVCDSDPLERKYKRS
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LTTAAAHLHYRDTRIYLLDTPGYPDFSGLAISALAAVETAAIVINAQTGIEMTTRRAMAW
HHHHHHHEEEECEEEEEEECCCCCCCCHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHH
AQARQLCRMIIVNGIDGDKVDLPALLADIQEAFGKECLPINLPAGNGSQVVDCFFNPAGE
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCC
SDFLSVPAAHDALIDQVIEIDPELMELYLEQGEAITPEQLHAPFERALREGHLVPICFTS
CCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
AATGAGITELLDVFVQLLPNPTEGNPPLFYRDTGDGSEHRKAVRAEPVPDKHVLAHVFKV
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHH
VIDPYVGKLAVFRIHQGTVTRDSQLYIGDGRQPFKVAHLLRLQGKETQEIPRAGPGDICA
HHHHHHCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCCCCCCEEE
VAKVDELGFDAVLHDATEDGNIHLTPLEFPTPIYGLAIEPARRGNEQRLAEVLHKLSAED
EEEHHHCCCHHHHCCCCCCCCEEEEECCCCCCEEEEEECHHHCCCHHHHHHHHHHHCCCC
PCLRVEHPVGTNETVVFGLGEFHLRCALERLTEQYKLEVVTRPPKIAYRETIGGKAEGHH
CCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHCCCCCCCCH
RHKKQTGGAGQFGEVMLRVEPLARGEGFDFVDAVKGGAIPGQFIPAVEKGIRQVLDSGPL
HHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
AGFPMQDVRVTVYDGKSHPVDSKEVAFATAGRKAFIDAVLKARPSVLEPIVDIEVTAPGT
CCCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCEEEEEECCCC
AMGDIIGDLSTKRGQVHGTRTAVGNAVVVAGQVPLSELNDYQSRLNSITGGHGSYTIQFS
HHHHHHHHCCCCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
HYDNVPPAQQDKMASKHKAQQDTD
CCCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHDSPDAIRTVALLGHAGCGKTSLVEALLHKGGALHTPGSVERGSTVCDSDPLERKYKRS
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LTTAAAHLHYRDTRIYLLDTPGYPDFSGLAISALAAVETAAIVINAQTGIEMTTRRAMAW
HHHHHHHEEEECEEEEEEECCCCCCCCHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHH
AQARQLCRMIIVNGIDGDKVDLPALLADIQEAFGKECLPINLPAGNGSQVVDCFFNPAGE
HHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCC
SDFLSVPAAHDALIDQVIEIDPELMELYLEQGEAITPEQLHAPFERALREGHLVPICFTS
CCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECC
AATGAGITELLDVFVQLLPNPTEGNPPLFYRDTGDGSEHRKAVRAEPVPDKHVLAHVFKV
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHH
VIDPYVGKLAVFRIHQGTVTRDSQLYIGDGRQPFKVAHLLRLQGKETQEIPRAGPGDICA
HHHHHHCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHCCCCCCCCEEE
VAKVDELGFDAVLHDATEDGNIHLTPLEFPTPIYGLAIEPARRGNEQRLAEVLHKLSAED
EEEHHHCCCHHHHCCCCCCCCEEEEECCCCCCEEEEEECHHHCCCHHHHHHHHHHHCCCC
PCLRVEHPVGTNETVVFGLGEFHLRCALERLTEQYKLEVVTRPPKIAYRETIGGKAEGHH
CCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHCCCCCCCCH
RHKKQTGGAGQFGEVMLRVEPLARGEGFDFVDAVKGGAIPGQFIPAVEKGIRQVLDSGPL
HHHHCCCCCCCCCEEEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
AGFPMQDVRVTVYDGKSHPVDSKEVAFATAGRKAFIDAVLKARPSVLEPIVDIEVTAPGT
CCCCCCCCEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHCCCEEEEEECCCC
AMGDIIGDLSTKRGQVHGTRTAVGNAVVVAGQVPLSELNDYQSRLNSITGGHGSYTIQFS
HHHHHHHHCCCCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEC
HYDNVPPAQQDKMASKHKAQQDTD
CCCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]