BacMap

Definition	Ralstonia eutropha H16 chromosome 2, complete sequence.
Accession	NC_008314
Length	2,912,490

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The map label for this gene is caiD [H]

Identifier: 116695841

GI number: 116695841

Start: 2151757

End: 2152560

Strand: Direct

Name: caiD [H]

Synonym: H16_B1905

Alternate gene names: 116695841

Gene position: 2151757-2152560 (Clockwise)

Preceding gene: 116695840

Following gene: 116695842

Centisome position: 73.88

GC content: 70.4

Gene sequence:

>804_bases
ATGACCGTACGACTCCACACCGAAGGCGCGGTCGCCGTGCTAACGATCGACCGGCCCGATGCCCTCAATGCGCTGGACGT
GGCCGCGCTGCGCGAACTGCGCGCGCACCTCATCGAAGTGCGCGACCGCGACGACCTGCGCGTGGCGGTGCTGACCGGCG
CCGGCACGCGCGCCTTCTGCGCGGGCGCCGACCTGAAAGGCACGCGCAGCTCGCCCGCGACCTATCCGGAAGCACTGTTC
CGCGCGCCGGAGCAGGCCGCCGACCTGGGGCTCTATATCCGGCTGATGGACCTGACCGACCTGGGCCTGTTCAAGCCGCT
GATCGCGGCGGTCAACGGCCATTGCCTTGGAGCGGGGCTTGAGATAGCGCTGCAATGCGATGCGCGGATCGCGTCAGAAC
AAGCGAGCTTCGGCCTGCCCGAAGCCGCGGTCGGGTCAATCCCCGCTGTCTCGGGCTTGCATCGCCTGCTGAAGGCCGTG
CCGGCGGCGCATGCGATGCAGATGGTGCTGACCGGCGAGCGAATCGACGCGGCGCAGGCCGCGCGCATCGGGCTGGTCAC
CGAGACGGTGGCGCCTGCCACGCTGCTCGACCGTGCGCTGGCGATCGCGACCCGCATCGCCGCCAATGCGCCGCTGGCGG
TGCAGGCCGTCAAGAAGCTGTCGCGCCAGACCAGCCACCTCAGCGAAGCCGACGCCCAGCAACTGACCGAACTCTACTGG
GGCGTGCTGCGCGATACCGCGGACCGCACCGAAGGCCGGCAGGCCTTCGCCGAGAAGCGCGAGCCCCGCTACACCGGGCG
CTAA

Upstream 100 bases:

>100_bases
GCTTCTTCCTCGACCCGCAGTCGTCCTACGTGACCGGGCAGGTGCTCTATGTCTGTGGCGGCCGCAGCCTGTCGTCGCCA
AGCGTGTAAGGAGGCAAGCC

Downstream 100 bases:

>100_bases
GCGTTCGCAAACAGAAAGACAGGAGACAGACCATGACCCGCATTACCCGATTCGCACGCGTGGCGCTGGCCGGCATGCTG
GCAGCCACGGCGCTGGGCGC

Product: enoyl-CoA hydratase/carnithine racemase

Products: crotonoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTVRLHTEGAVAVLTIDRPDALNALDVAALRELRAHLIEVRDRDDLRVAVLTGAGTRAFCAGADLKGTRSSPATYPEALF
RAPEQAADLGLYIRLMDLTDLGLFKPLIAAVNGHCLGAGLEIALQCDARIASEQASFGLPEAAVGSIPAVSGLHRLLKAV
PAAHAMQMVLTGERIDAAQAARIGLVTETVAPATLLDRALAIATRIAANAPLAVQAVKKLSRQTSHLSEADAQQLTELYW
GVLRDTADRTEGRQAFAEKREPRYTGR

Sequences:

>Translated_267_residues
MTVRLHTEGAVAVLTIDRPDALNALDVAALRELRAHLIEVRDRDDLRVAVLTGAGTRAFCAGADLKGTRSSPATYPEALF
RAPEQAADLGLYIRLMDLTDLGLFKPLIAAVNGHCLGAGLEIALQCDARIASEQASFGLPEAAVGSIPAVSGLHRLLKAV
PAAHAMQMVLTGERIDAAQAARIGLVTETVAPATLLDRALAIATRIAANAPLAVQAVKKLSRQTSHLSEADAQQLTELYW
GVLRDTADRTEGRQAFAEKREPRYTGR
>Mature_266_residues
TVRLHTEGAVAVLTIDRPDALNALDVAALRELRAHLIEVRDRDDLRVAVLTGAGTRAFCAGADLKGTRSSPATYPEALFR
APEQAADLGLYIRLMDLTDLGLFKPLIAAVNGHCLGAGLEIALQCDARIASEQASFGLPEAAVGSIPAVSGLHRLLKAVP
AAHAMQMVLTGERIDAAQAARIGLVTETVAPATLLDRALAIATRIAANAPLAVQAVKKLSRQTSHLSEADAQQLTELYWG
VLRDTADRTEGRQAFAEKREPRYTGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=34.6456692913386, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI20127408, Length=193, Percent_Identity=33.160621761658, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI31542718, Length=264, Percent_Identity=28.7878787878788, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502327, Length=266, Percent_Identity=28.1954887218045, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI37594469, Length=194, Percent_Identity=27.319587628866, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI37594471, Length=194, Percent_Identity=27.319587628866, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI68989263, Length=234, Percent_Identity=30.3418803418803, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=28.0155642023346, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI150378541, Length=263, Percent_Identity=27.7566539923954, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=247, Percent_Identity=26.7206477732794, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI260275230, Length=247, Percent_Identity=26.7206477732794, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI260274832, Length=247, Percent_Identity=26.7206477732794, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI221307494, Length=246, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI221136756, Length=245, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI221136753, Length=245, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI213417737, Length=248, Percent_Identity=23.7903225806452, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI157694516, Length=248, Percent_Identity=23.7903225806452, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI25453481, Length=247, Percent_Identity=23.4817813765182, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=247, Percent_Identity=23.4817813765182, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI51538573, Length=247, Percent_Identity=23.4817813765182, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI51538581, Length=247, Percent_Identity=23.4817813765182, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI116235487, Length=218, Percent_Identity=26.605504587156, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI221142681, Length=272, Percent_Identity=38.2352941176471, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=40, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1788597, Length=259, Percent_Identity=29.3436293436293, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=33.4928229665072, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1787660, Length=272, Percent_Identity=33.0882352941176, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1790281, Length=248, Percent_Identity=30.6451612903226, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87082183, Length=271, Percent_Identity=25.0922509225092, Blast_Score=84, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=37.109375, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=34.4961240310078, Blast_Score=136, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=32.3144104803493, Blast_Score=109, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17558304, Length=201, Percent_Identity=31.8407960199005, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=263, Percent_Identity=27.7566539923954, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=28.9592760180996, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17508953, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17508951, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25144160, Length=216, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=216, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17534483, Length=222, Percent_Identity=29.2792792792793, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540306, Length=255, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=220, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=192, Percent_Identity=26.0416666666667, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=35.9375, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=35.9375, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24653139, Length=261, Percent_Identity=31.4176245210728, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24650670, Length=209, Percent_Identity=30.622009569378, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28571729, Length=193, Percent_Identity=26.4248704663212, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571730, Length=193, Percent_Identity=26.4248704663212, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=28.6363636363636, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922422, Length=273, Percent_Identity=27.1062271062271, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920382, Length=209, Percent_Identity=30.622009569378, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921000, Length=193, Percent_Identity=34.1968911917098, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583077, Length=193, Percent_Identity=34.1968911917098, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583079, Length=193, Percent_Identity=34.1968911917098, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24583165, Length=238, Percent_Identity=25.2100840336134, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.55

Molecular weight: Translated: 28426; Mature: 28295

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLHTEGAVAVLTIDRPDALNALDVAALRELRAHLIEVRDRDDLRVAVLTGAGTRAFC
CEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
AGADLKGTRSSPATYPEALFRAPEQAADLGLYIRLMDLTDLGLFKPLIAAVNGHCLGAGL
CCCCCCCCCCCCCCCHHHHHCCCHHHHCCEEEEEEECHHHHHHHHHHHHHHCCCEECCCC
EIALQCDARIASEQASFGLPEAAVGSIPAVSGLHRLLKAVPAAHAMQMVLTGERIDAAQA
EEEEEECCHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
ARIGLVTETVAPATLLDRALAIATRIAANAPLAVQAVKKLSRQTSHLSEADAQQLTELYW
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLRDTADRTEGRQAFAEKREPRYTGR
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVRLHTEGAVAVLTIDRPDALNALDVAALRELRAHLIEVRDRDDLRVAVLTGAGTRAFC
EEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
AGADLKGTRSSPATYPEALFRAPEQAADLGLYIRLMDLTDLGLFKPLIAAVNGHCLGAGL
CCCCCCCCCCCCCCCHHHHHCCCHHHHCCEEEEEEECHHHHHHHHHHHHHHCCCEECCCC
EIALQCDARIASEQASFGLPEAAVGSIPAVSGLHRLLKAVPAAHAMQMVLTGERIDAAQA
EEEEEECCHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
ARIGLVTETVAPATLLDRALAIATRIAANAPLAVQAVKKLSRQTSHLSEADAQQLTELYW
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVLRDTADRTEGRQAFAEKREPRYTGR
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxybutanoyl-CoA

Specific reaction: (3R)-3-hydroxybutanoyl-CoA = crotonoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA