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The map label for this gene is garR [C]

Identifier: 116695596

GI number: 116695596

Start: 1868077

End: 1868955

Strand: Direct

Name: garR [C]

Synonym: H16_B1657

Alternate gene names: 116695596

Gene position: 1868077-1868955 (Clockwise)

Preceding gene: 116695592

Following gene: 116695597

Centisome position: 64.14

GC content: 69.4

Gene sequence:

>879_bases
ATGCGTGTCGCATTTCTCGGACTGGGCGTCATGGGTTTCCACATGGCCGGCCACCTCGCCACCAAGGGGCACGAGGTCAC
CGTGTACAACCGCACCGCCGCCAAGGCGCAGGCCTGGGTCGGGAAGTTCGGCGGCAAAGCGGCTCCCACGCCGGCACAGG
CCGTGCGCGATGCGCAGGTGGTCTGCTCCTGCGTGGGCAATGACGACGACCTGCGCGCGGTGCTGACCGGGCATGACGGC
GCCTTCTTCAGCGCGCCCAGCGGCTGCATTTTCGTGGACCACACCACCGCCAGCGCCAACGTGGCGCGCGAACTCCACGC
CGCCGCGCGCGAGCACGGCCTGCACTTTGTCGACGGCCCGGTCTCCGGCGGCGAAGTCGGCGCGGAGAAAGGCATCCTGA
CCATCATGTGCGGCGGCGATGCCGACGCCTATGCGCGCGCCGAACCGGTCATTGCCGCCTACGCACGCGCCGTCACGCGC
ATCGGCGAATCCGGCGCCGGGCAACTGGCCAAGATGGTCAACCAGATCAGCATCGCCGGGCTGATCCAGGGCCTGTCCGA
GGCCATCGCCTTTGGCGAGCGCGCCGGGCTGGACATGCGCCTAGTGCTGGACGTCATCAGCAAGGGCGCGGCCGGCTCAT
GGCAGCTCGAGAACCGCGGCCCCACCATGATCGACAACAAGTTCGACTTCGGCTTCGCGGTGGACTGGATGCGCAAGGAC
CTGGGCCTGTGCCTGGACGAGGCGCGCCGCAACGGCGCCGGCCTGCCGGTGACGGCGCTGGTCGACCAGTTCTATGCCGA
CCTGCAGCAGATGGGCTGCGGCCGCGCCGACACTTCTTCGCTGATCAAGCGCCTGCGCCAGCACGCCGGCGTCGCCTGA

Upstream 100 bases:

>100_bases
GGCGGCCCAGCCGTCCCGCCGGCGGAGGACCCTGGCGTGGCATCACGCAAACGGGGCCGCGCCTCACCGATCCCAAACAT
ACAAGCAACGGAGCAGCAGC

Downstream 100 bases:

>100_bases
CGCCACCGCTGCGCCACCTGCGTCGTCCGATTCCTACAGCGCAATCGGCGTGTCTCGGAGAGGCCCCTGCCCGGCATGGG
CCTAAGCTGGACACATGGGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MRVAFLGLGVMGFHMAGHLATKGHEVTVYNRTAAKAQAWVGKFGGKAAPTPAQAVRDAQVVCSCVGNDDDLRAVLTGHDG
AFFSAPSGCIFVDHTTASANVARELHAAAREHGLHFVDGPVSGGEVGAEKGILTIMCGGDADAYARAEPVIAAYARAVTR
IGESGAGQLAKMVNQISIAGLIQGLSEAIAFGERAGLDMRLVLDVISKGAAGSWQLENRGPTMIDNKFDFGFAVDWMRKD
LGLCLDEARRNGAGLPVTALVDQFYADLQQMGCGRADTSSLIKRLRQHAGVA

Sequences:

>Translated_292_residues
MRVAFLGLGVMGFHMAGHLATKGHEVTVYNRTAAKAQAWVGKFGGKAAPTPAQAVRDAQVVCSCVGNDDDLRAVLTGHDG
AFFSAPSGCIFVDHTTASANVARELHAAAREHGLHFVDGPVSGGEVGAEKGILTIMCGGDADAYARAEPVIAAYARAVTR
IGESGAGQLAKMVNQISIAGLIQGLSEAIAFGERAGLDMRLVLDVISKGAAGSWQLENRGPTMIDNKFDFGFAVDWMRKD
LGLCLDEARRNGAGLPVTALVDQFYADLQQMGCGRADTSSLIKRLRQHAGVA
>Mature_292_residues
MRVAFLGLGVMGFHMAGHLATKGHEVTVYNRTAAKAQAWVGKFGGKAAPTPAQAVRDAQVVCSCVGNDDDLRAVLTGHDG
AFFSAPSGCIFVDHTTASANVARELHAAAREHGLHFVDGPVSGGEVGAEKGILTIMCGGDADAYARAEPVIAAYARAVTR
IGESGAGQLAKMVNQISIAGLIQGLSEAIAFGERAGLDMRLVLDVISKGAAGSWQLENRGPTMIDNKFDFGFAVDWMRKD
LGLCLDEARRNGAGLPVTALVDQFYADLQQMGCGRADTSSLIKRLRQHAGVA

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=28.6689419795222, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI40556376, Length=283, Percent_Identity=25.0883392226148, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI145693186, Length=281, Percent_Identity=34.1637010676157, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=32.1799307958478, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790315, Length=292, Percent_Identity=30.4794520547945, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789092, Length=221, Percent_Identity=35.2941176470588, Blast_Score=101, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=28.2527881040892, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24655230, Length=301, Percent_Identity=26.578073089701, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922568, Length=301, Percent_Identity=26.578073089701, Blast_Score=115, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=23.6749116607774, Blast_Score=102, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30596; Mature: 30596

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAFLGLGVMGFHMAGHLATKGHEVTVYNRTAAKAQAWVGKFGGKAAPTPAQAVRDAQV
CEEEEEEHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VCSCVGNDDDLRAVLTGHDGAFFSAPSGCIFVDHTTASANVARELHAAAREHGLHFVDGP
HHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCC
VSGGEVGAEKGILTIMCGGDADAYARAEPVIAAYARAVTRIGESGAGQLAKMVNQISIAG
CCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LIQGLSEAIAFGERAGLDMRLVLDVISKGAAGSWQLENRGPTMIDNKFDFGFAVDWMRKD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCCHHHHHHHHH
LGLCLDEARRNGAGLPVTALVDQFYADLQQMGCGRADTSSLIKRLRQHAGVA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVAFLGLGVMGFHMAGHLATKGHEVTVYNRTAAKAQAWVGKFGGKAAPTPAQAVRDAQV
CEEEEEEHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VCSCVGNDDDLRAVLTGHDGAFFSAPSGCIFVDHTTASANVARELHAAAREHGLHFVDGP
HHHHCCCCCCEEEEEECCCCCEEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEECCC
VSGGEVGAEKGILTIMCGGDADAYARAEPVIAAYARAVTRIGESGAGQLAKMVNQISIAG
CCCCCCCCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LIQGLSEAIAFGERAGLDMRLVLDVISKGAAGSWQLENRGPTMIDNKFDFGFAVDWMRKD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCCCCEECCCCCCCHHHHHHHHH
LGLCLDEARRNGAGLPVTALVDQFYADLQQMGCGRADTSSLIKRLRQHAGVA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA