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The map label for this gene is alkJ [H]

Identifier: 116695473

GI number: 116695473

Start: 1719917

End: 1721617

Strand: Direct

Name: alkJ [H]

Synonym: H16_B1532

Alternate gene names: 116695473

Gene position: 1719917-1721617 (Clockwise)

Preceding gene: 116695471

Following gene: 116695474

Centisome position: 59.05

GC content: 68.2

Gene sequence:

>1701_bases
ATGCTGGCAACTCAGGAGACACGCAAATTGAATACCAAGACATCACAAGACACCGCCAGCGCGGCGGCAGAGTTCGACTA
CATCGTGATCGGCGCGGGATCGGCGGGGTGCGCCGTGGCGGGGCGCCTGGCAGAAGATCCGAGTGCGACCGTGGCGCTGC
TCGAGGCCGGGCCGCATGATCATCACTTCTCGATCTGGGCGCCGGTGGGCATTGCCGCGGTGGTGCCCAAGGCCGGCCCG
CGCAACTACGCCTACTACACGGTACCGCAACCGGGCCTGAACGGCCGCCGCTCCTACCAGCCGCGCGGGCGCGGCCTGGG
CGGCAGCTCCTCGATCAACGGCATGGTCTATATCCGCGGCCATCGGCGCGACTACGACGACTGGGCCGCGCTCGGTTGCC
GGGGCTGGGGCTTCGACGACGTCCTGCCCTACTTCCGCCGCAGCGAGCGCAATCCTAGCCTGGCCGGCCAGGAACATCCG
CTGCACGGCAATGACGGGCCGCTGCACGTGAGCGACCTGCGCTCGCCCAATCCGTTTGCACAGCGCTTTGTCCAGGCAGC
GATCCAGGCCGGCCTGCCTCACAACGACGACTTCAATGGCCACAGCCAGGAAGGCGTCGGCCTGTACCAGGTGACGCAGC
GCAACGGCGAGCGCTGGAACTCGGCGCGGGCCTACCTGCACAACGGCAATGCCGCGGACACGGCGCTCAATGGCGGCCGT
CGCGGCCTGGCGGTGCTGACCGATACGCAAGCGCTGCGCATCGTGTTCGAAGGCAGGCGCGCTGTCGGCGTGGAAGTCGT
GCGCGGCGGGGCGGTGCAGACCCTGCGCGCACGGCGCGAGATCGTGGTCAGCGGCGGCACCTTCAACTCGCCGCAGTTGC
TGCTGGCATCCGGCATCGGCCCGGCCGCGCACTTGCGCAACTTCGGCATCGACGTGGTCCATGACCTTCCGGGCGTGGGC
GAGAACCTGCAGGACCACCTCGACATCATCGTCAACAAGCAGCTGCAAACGACCGAGCTGTTCGGCAAGACCGGCCGCGG
CATGTTGCGGCTGGCGCGCGAGGTGATGCGCTACCGGCGCAACCGCACCGGCATGGTGTCGTCCAATATCGCCGAGGCCG
GTGCGTTCCTGCGCAGCCGCCCCGAGCTGGACATCCCGGATGTGCAGCTGCACTTTGCCGTGGCGCTGCTCGGCAACCGC
AACCTGGGCAAACTGGGCCACGGCTACTCCTGCCACGCCTGCGTGCTGCGTCCGAAGAGCCGCGGCCATGTGCGCCTGCG
CAGCGCCGACATGCGCGAGACGCCGGTGATCGATCCGCGCTTCCTGTCGGCTGAGGAAGACATGGCCGGCATGGTCGCAG
GCGTGCGGGCAATCCGCCGCATCTTTGCGCAGCCTGCGCTGGCGCAGCACGGCGGGCGCGAAATCCTGACCGACGCTTTC
GGCCCCGACAGCAGCAATGAAGCCGCCATCCGCGACTTCGTGCGCACGCACGCGGACACGGTCTACCACCCGGTCGGCAC
CTGCAAGATGGGCGTCGACAACATGGCGGTAGTCGACCCGGAACTACGCGTGCAAGGTGTGGAGGGCCTGCGCGTCGCTG
ACGCATCGATCATGCCGACGCTGATCGGCGGCAACACCAACGCCCCCGCCATCATGATCGGCGAAAAGGCTGCGGACCTG
GTCAAGGCCTCGCACCGCTAA

Upstream 100 bases:

>100_bases
CTATCATGACAGCCTGCGGCGTAACCCTATTGACCTGTTATAACAGGTAATGGCAGTATCCGTCGTCACCAGATATGCGC
GCGCCCCCGCAGATCACCGC

Downstream 100 bases:

>100_bases
GCCGGCTATCCCCCTGCATTGGCAACCACTACACCGAGGAGACACCCCATGCAAGCCACTGCCGCAACCACCATCAGCCC
GGAAGCCTACCGCGCGCTGG

Product: oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MLATQETRKLNTKTSQDTASAAAEFDYIVIGAGSAGCAVAGRLAEDPSATVALLEAGPHDHHFSIWAPVGIAAVVPKAGP
RNYAYYTVPQPGLNGRRSYQPRGRGLGGSSSINGMVYIRGHRRDYDDWAALGCRGWGFDDVLPYFRRSERNPSLAGQEHP
LHGNDGPLHVSDLRSPNPFAQRFVQAAIQAGLPHNDDFNGHSQEGVGLYQVTQRNGERWNSARAYLHNGNAADTALNGGR
RGLAVLTDTQALRIVFEGRRAVGVEVVRGGAVQTLRARREIVVSGGTFNSPQLLLASGIGPAAHLRNFGIDVVHDLPGVG
ENLQDHLDIIVNKQLQTTELFGKTGRGMLRLAREVMRYRRNRTGMVSSNIAEAGAFLRSRPELDIPDVQLHFAVALLGNR
NLGKLGHGYSCHACVLRPKSRGHVRLRSADMRETPVIDPRFLSAEEDMAGMVAGVRAIRRIFAQPALAQHGGREILTDAF
GPDSSNEAAIRDFVRTHADTVYHPVGTCKMGVDNMAVVDPELRVQGVEGLRVADASIMPTLIGGNTNAPAIMIGEKAADL
VKASHR

Sequences:

>Translated_566_residues
MLATQETRKLNTKTSQDTASAAAEFDYIVIGAGSAGCAVAGRLAEDPSATVALLEAGPHDHHFSIWAPVGIAAVVPKAGP
RNYAYYTVPQPGLNGRRSYQPRGRGLGGSSSINGMVYIRGHRRDYDDWAALGCRGWGFDDVLPYFRRSERNPSLAGQEHP
LHGNDGPLHVSDLRSPNPFAQRFVQAAIQAGLPHNDDFNGHSQEGVGLYQVTQRNGERWNSARAYLHNGNAADTALNGGR
RGLAVLTDTQALRIVFEGRRAVGVEVVRGGAVQTLRARREIVVSGGTFNSPQLLLASGIGPAAHLRNFGIDVVHDLPGVG
ENLQDHLDIIVNKQLQTTELFGKTGRGMLRLAREVMRYRRNRTGMVSSNIAEAGAFLRSRPELDIPDVQLHFAVALLGNR
NLGKLGHGYSCHACVLRPKSRGHVRLRSADMRETPVIDPRFLSAEEDMAGMVAGVRAIRRIFAQPALAQHGGREILTDAF
GPDSSNEAAIRDFVRTHADTVYHPVGTCKMGVDNMAVVDPELRVQGVEGLRVADASIMPTLIGGNTNAPAIMIGEKAADL
VKASHR
>Mature_566_residues
MLATQETRKLNTKTSQDTASAAAEFDYIVIGAGSAGCAVAGRLAEDPSATVALLEAGPHDHHFSIWAPVGIAAVVPKAGP
RNYAYYTVPQPGLNGRRSYQPRGRGLGGSSSINGMVYIRGHRRDYDDWAALGCRGWGFDDVLPYFRRSERNPSLAGQEHP
LHGNDGPLHVSDLRSPNPFAQRFVQAAIQAGLPHNDDFNGHSQEGVGLYQVTQRNGERWNSARAYLHNGNAADTALNGGR
RGLAVLTDTQALRIVFEGRRAVGVEVVRGGAVQTLRARREIVVSGGTFNSPQLLLASGIGPAAHLRNFGIDVVHDLPGVG
ENLQDHLDIIVNKQLQTTELFGKTGRGMLRLAREVMRYRRNRTGMVSSNIAEAGAFLRSRPELDIPDVQLHFAVALLGNR
NLGKLGHGYSCHACVLRPKSRGHVRLRSADMRETPVIDPRFLSAEEDMAGMVAGVRAIRRIFAQPALAQHGGREILTDAF
GPDSSNEAAIRDFVRTHADTVYHPVGTCKMGVDNMAVVDPELRVQGVEGLRVADASIMPTLIGGNTNAPAIMIGEKAADL
VKASHR

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=549, Percent_Identity=43.7158469945355, Blast_Score=410, Evalue=1e-114,
Organism=Escherichia coli, GI1786503, Length=547, Percent_Identity=42.7787934186472, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17532301, Length=589, Percent_Identity=36.8421052631579, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24642042, Length=588, Percent_Identity=37.9251700680272, Blast_Score=332, Evalue=5e-91,
Organism=Drosophila melanogaster, GI45549471, Length=593, Percent_Identity=37.6053962900506, Blast_Score=331, Evalue=9e-91,
Organism=Drosophila melanogaster, GI45551458, Length=593, Percent_Identity=37.6053962900506, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24642055, Length=596, Percent_Identity=36.4093959731544, Blast_Score=325, Evalue=6e-89,
Organism=Drosophila melanogaster, GI24642039, Length=579, Percent_Identity=37.4784110535406, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI17137792, Length=573, Percent_Identity=37.8708551483421, Blast_Score=313, Evalue=2e-85,
Organism=Drosophila melanogaster, GI24650267, Length=603, Percent_Identity=34.9917081260365, Blast_Score=311, Evalue=6e-85,
Organism=Drosophila melanogaster, GI18859995, Length=589, Percent_Identity=35.6536502546689, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24642035, Length=581, Percent_Identity=35.9724612736661, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24642059, Length=586, Percent_Identity=34.8122866894198, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24642048, Length=584, Percent_Identity=34.2465753424658, Blast_Score=297, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24642037, Length=584, Percent_Identity=35.2739726027397, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI18859993, Length=585, Percent_Identity=32.4786324786325, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642051, Length=582, Percent_Identity=31.0996563573883, Blast_Score=261, Evalue=6e-70,
Organism=Drosophila melanogaster, GI24645930, Length=591, Percent_Identity=27.580372250423, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24642057, Length=601, Percent_Identity=29.450915141431, Blast_Score=153, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 61103; Mature: 61103

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATQETRKLNTKTSQDTASAAAEFDYIVIGAGSAGCAVAGRLAEDPSATVALLEAGPHD
CCCCCCHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHCCCCCCCCCEEEEEECCCCC
HHFSIWAPVGIAAVVPKAGPRNYAYYTVPQPGLNGRRSYQPRGRGLGGSSSINGMVYIRG
CCEEEEECCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
HRRDYDDWAALGCRGWGFDDVLPYFRRSERNPSLAGQEHPLHGNDGPLHVSDLRSPNPFA
CCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHCCCCCCHHH
QRFVQAAIQAGLPHNDDFNGHSQEGVGLYQVTQRNGERWNSARAYLHNGNAADTALNGGR
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHCCCC
RGLAVLTDTQALRIVFEGRRAVGVEVVRGGAVQTLRARREIVVSGGTFNSPQLLLASGIG
CCEEEEECCCEEEEEEECCCEEEEEEECCCHHHHHHHCEEEEEECCCCCCCCEEEECCCC
PAAHLRNFGIDVVHDLPGVGENLQDHLDIIVNKQLQTTELFGKTGRGMLRLAREVMRYRR
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHC
NRTGMVSSNIAEAGAFLRSRPELDIPDVQLHFAVALLGNRNLGKLGHGYSCHACVLRPKS
CCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCC
RGHVRLRSADMRETPVIDPRFLSAEEDMAGMVAGVRAIRRIFAQPALAQHGGREILTDAF
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCC
GPDSSNEAAIRDFVRTHADTVYHPVGTCKMGVDNMAVVDPELRVQGVEGLRVADASIMPT
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCEEECCCCEEEECCCCCCE
LIGGNTNAPAIMIGEKAADLVKASHR
EECCCCCCCEEEECCHHHHHHHHCCC
>Mature Secondary Structure
MLATQETRKLNTKTSQDTASAAAEFDYIVIGAGSAGCAVAGRLAEDPSATVALLEAGPHD
CCCCCCHHHCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHCCCCCCCCCEEEEEECCCCC
HHFSIWAPVGIAAVVPKAGPRNYAYYTVPQPGLNGRRSYQPRGRGLGGSSSINGMVYIRG
CCEEEEECCCEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEC
HRRDYDDWAALGCRGWGFDDVLPYFRRSERNPSLAGQEHPLHGNDGPLHVSDLRSPNPFA
CCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHCCCCCCHHH
QRFVQAAIQAGLPHNDDFNGHSQEGVGLYQVTQRNGERWNSARAYLHNGNAADTALNGGR
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHCCCC
RGLAVLTDTQALRIVFEGRRAVGVEVVRGGAVQTLRARREIVVSGGTFNSPQLLLASGIG
CCEEEEECCCEEEEEEECCCEEEEEEECCCHHHHHHHCEEEEEECCCCCCCCEEEECCCC
PAAHLRNFGIDVVHDLPGVGENLQDHLDIIVNKQLQTTELFGKTGRGMLRLAREVMRYRR
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHC
NRTGMVSSNIAEAGAFLRSRPELDIPDVQLHFAVALLGNRNLGKLGHGYSCHACVLRPKS
CCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCC
RGHVRLRSADMRETPVIDPRFLSAEEDMAGMVAGVRAIRRIFAQPALAQHGGREILTDAF
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHCC
GPDSSNEAAIRDFVRTHADTVYHPVGTCKMGVDNMAVVDPELRVQGVEGLRVADASIMPT
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEECCCCEEECCCCEEEECCCCCCE
LIGGNTNAPAIMIGEKAADLVKASHR
EECCCCCCCEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]