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The map label for this gene is gcvA [H]

Identifier: 116695449

GI number: 116695449

Start: 1694374

End: 1695306

Strand: Direct

Name: gcvA [H]

Synonym: H16_B1508

Alternate gene names: 116695449

Gene position: 1694374-1695306 (Clockwise)

Preceding gene: 116695448

Following gene: 116695452

Centisome position: 58.18

GC content: 70.2

Gene sequence:

>933_bases
ATGGCGGCTTCTCTGACCGGAAACCCCATGCGCATTTCCCCGCTCCCGCCCCTGCCCAACCTGGTCGCGTTTGAAGCCGC
GATGCGCCACGGCAGCTTCACCCGCGCCGCGGCCGAACTGCACCTGACCCAAAGCGCGATCAGCCGCCAGGTGGCCCAGC
TGGAAGACTTTCTCGGGCGCAAGCTCTTTATCCGCGAACCGCGCGCGCTGCGCCTGACCGTCAGTGGCCAGCGCTATGCC
GAGGTGGTGCAGCGCCTGCTGGTGGGCTGCGCCGAAGCGACCGAAGACGTGATGAAGGTGCGCAGCCATACCGCCCTGAC
GGTGGCGTGCTCCAGCGGCGTGGCGGTGCTGTGGCTGACGCCGCGGCTGGCCTCGTTCCGCGAGGCGCACCCCGAGATCC
AGCTGCGCCTGACCGTCAACGACAGCCTGGCGTCGCTGTCGCCGGCGGAGTTCGATATCGGCATGTACTACCTGCGCGAG
GGCCCGCCGCCGGGACTGGCAGCACGACGGCTGTATGGCGAGGAAGTGTTCCCGGTGTGCGCGCCGGGCTACCTGAACGG
CCGCACGCTGGCCCCGGCCGCGCTGGCCAATGAAACGCTGCTGATGCTCGACGACGGCCAGCGCCAGTGGATGTCCTGGC
AGACCTGGCTGGCCAACCAGGGCCTGCCCGACGCCACGCTGCGCAACGTGCTCACCTCCAACCAGTACCCGATCCTGCTG
CAGCTGGCCATGGAAGGCCATGGCATCGTACTGGGCTGGCGCCATATGATCGACGCCTGCCTGCGCGAAGGCCTGCTGGT
GCGCGCCAGCGAGGCCAGCGCCAGCCTGGGCGGCGGCTACTACGCGGTGTGGCCGCGCGACCGGATGGAACCGGCCGCGG
CGCGCGCGTTCCGCAGCTGGCTGGTGCGGCAGGCGACGCCTGCACCAGCCTGA

Upstream 100 bases:

>100_bases
AGCGTGGTGATGCAGGCCGGCCGCATCCACCGCCGCGCCGGCTGATCCGGGCGGCGCGCCCCTCCCGCTGGCCTCCATCT
GGATGCGCCGGCGGTCTATC

Downstream 100 bases:

>100_bases
TCGCTCAGGAACCCGCCTTGTAGTTCGTATCCTTGACGATCTTCGCCCACCTTGCCGCATCCTCGGCAATGGTCGCGGCA
AACTGCTCCGGCGTGCCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MAASLTGNPMRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLEDFLGRKLFIREPRALRLTVSGQRYA
EVVQRLLVGCAEATEDVMKVRSHTALTVACSSGVAVLWLTPRLASFREAHPEIQLRLTVNDSLASLSPAEFDIGMYYLRE
GPPPGLAARRLYGEEVFPVCAPGYLNGRTLAPAALANETLLMLDDGQRQWMSWQTWLANQGLPDATLRNVLTSNQYPILL
QLAMEGHGIVLGWRHMIDACLREGLLVRASEASASLGGGYYAVWPRDRMEPAAARAFRSWLVRQATPAPA

Sequences:

>Translated_310_residues
MAASLTGNPMRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLEDFLGRKLFIREPRALRLTVSGQRYA
EVVQRLLVGCAEATEDVMKVRSHTALTVACSSGVAVLWLTPRLASFREAHPEIQLRLTVNDSLASLSPAEFDIGMYYLRE
GPPPGLAARRLYGEEVFPVCAPGYLNGRTLAPAALANETLLMLDDGQRQWMSWQTWLANQGLPDATLRNVLTSNQYPILL
QLAMEGHGIVLGWRHMIDACLREGLLVRASEASASLGGGYYAVWPRDRMEPAAARAFRSWLVRQATPAPA
>Mature_309_residues
AASLTGNPMRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLEDFLGRKLFIREPRALRLTVSGQRYAE
VVQRLLVGCAEATEDVMKVRSHTALTVACSSGVAVLWLTPRLASFREAHPEIQLRLTVNDSLASLSPAEFDIGMYYLREG
PPPGLAARRLYGEEVFPVCAPGYLNGRTLAPAALANETLLMLDDGQRQWMSWQTWLANQGLPDATLRNVLTSNQYPILLQ
LAMEGHGIVLGWRHMIDACLREGLLVRASEASASLGGGYYAVWPRDRMEPAAARAFRSWLVRQATPAPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=36.518771331058, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI1786448, Length=302, Percent_Identity=32.4503311258278, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788706, Length=298, Percent_Identity=29.1946308724832, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=30, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1790262, Length=290, Percent_Identity=28.2758620689655, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1787589, Length=300, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI157672245, Length=143, Percent_Identity=34.2657342657343, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789440, Length=240, Percent_Identity=25.8333333333333, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081978, Length=246, Percent_Identity=26.8292682926829, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI2367136, Length=236, Percent_Identity=26.271186440678, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=26.1224489795918, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=26.3698630136986, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34040; Mature: 33909

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASLTGNPMRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLEDFLGR
CCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KLFIREPRALRLTVSGQRYAEVVQRLLVGCAEATEDVMKVRSHTALTVACSSGVAVLWLT
EEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEC
PRLASFREAHPEIQLRLTVNDSLASLSPAEFDIGMYYLREGPPPGLAARRLYGEEVFPVC
CCHHHHHHCCCCEEEEEEECCCHHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEE
APGYLNGRTLAPAALANETLLMLDDGQRQWMSWQTWLANQGLPDATLRNVLTSNQYPILL
CCCCCCCCCCCCHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEE
QLAMEGHGIVLGWRHMIDACLREGLLVRASEASASLGGGYYAVWPRDRMEPAAARAFRSW
EEEECCCEEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCEEEECCHHHCCHHHHHHHHHH
LVRQATPAPA
HHHCCCCCCC
>Mature Secondary Structure 
AASLTGNPMRISPLPPLPNLVAFEAAMRHGSFTRAAAELHLTQSAISRQVAQLEDFLGR
CCCCCCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KLFIREPRALRLTVSGQRYAEVVQRLLVGCAEATEDVMKVRSHTALTVACSSGVAVLWLT
EEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEC
PRLASFREAHPEIQLRLTVNDSLASLSPAEFDIGMYYLREGPPPGLAARRLYGEEVFPVC
CCHHHHHHCCCCEEEEEEECCCHHCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEE
APGYLNGRTLAPAALANETLLMLDDGQRQWMSWQTWLANQGLPDATLRNVLTSNQYPILL
CCCCCCCCCCCCHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEE
QLAMEGHGIVLGWRHMIDACLREGLLVRASEASASLGGGYYAVWPRDRMEPAAARAFRSW
EEEECCCEEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCEEEECCHHHCCHHHHHHHHHH
LVRQATPAPA
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]