Click here to switch to the map view.

The map label for this gene is pucL [H]

Identifier: 116694943

GI number: 116694943

Start: 1124032

End: 1124550

Strand: Direct

Name: pucL [H]

Synonym: H16_B0998

Alternate gene names: 116694943

Gene position: 1124032-1124550 (Clockwise)

Preceding gene: 116694942

Following gene: 116694946

Centisome position: 38.59

GC content: 67.44

Gene sequence:

>519_bases
ATGTCTCAACCACTCGACCTGGCGTCGGTCAACGCCCTGGACGAAGCGGCCTTTGCCGATGCCTTCGGCAGCGTGTTCGA
ACACTTTCCGCAGGCTGCCGAGGGCGCCTGGGCGCAGCGGCCGTTCGCATCGGCAACGGCGCTGCATGGCGCCATGATGG
ACGTGGTGCGCAAGCTTGACGAACCGCGCCAGCGCGATTTCCTGAACCTGCACCCGCAGCTGTCGGCACGCAATATCCGC
GCGGGCACGATGACCGCCGATTCCAACGCCGAGCAGAAGAGCGCCGGCCTGGACGCGATGTCGGCGCAGCAAGAGGCCGC
GCTGGACCAGCTTAACGCGGACTACCAGGCGCGCCATGGCTTCCCGTTCATCATCTGCGTGCGCCACTACACCCGCGAAG
GCATCTTCGCCGCGTTCGAACGCCGGCTTGGCCGCAGCACGCAGCAGGAGCTTGATGAAGCGCTGGCACAGATCGCCGCG
ATCACGCGAGGGCGGCTGGCGGCGCGGCTGGCCGGTTGA

Upstream 100 bases:

>100_bases
CTATAGGGCGCGGGCTCTCGCCGCTGCGGCGGGCCGTTCCTATACTCGGCCCCGTATCCAATGATTCCCCATGCGCGGGA
TCCCTACGGGAGAAGCATCC

Downstream 100 bases:

>100_bases
GTCCAGCGCCTATCCAAAGGCCTCAAGCGCCAGCGCCAGCATCCCCACCAGGATGAGGCTGGCGGCCCAGACCGCCTCCA
GGTTGAACCAGCTCCGCGAT

Product: hypothetical protein

Products: NA

Alternate protein names: 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase; OHCU decarboxylase; Uricase; Urate oxidase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSQPLDLASVNALDEAAFADAFGSVFEHFPQAAEGAWAQRPFASATALHGAMMDVVRKLDEPRQRDFLNLHPQLSARNIR
AGTMTADSNAEQKSAGLDAMSAQQEAALDQLNADYQARHGFPFIICVRHYTREGIFAAFERRLGRSTQQELDEALAQIAA
ITRGRLAARLAG

Sequences:

>Translated_172_residues
MSQPLDLASVNALDEAAFADAFGSVFEHFPQAAEGAWAQRPFASATALHGAMMDVVRKLDEPRQRDFLNLHPQLSARNIR
AGTMTADSNAEQKSAGLDAMSAQQEAALDQLNADYQARHGFPFIICVRHYTREGIFAAFERRLGRSTQQELDEALAQIAA
ITRGRLAARLAG
>Mature_171_residues
SQPLDLASVNALDEAAFADAFGSVFEHFPQAAEGAWAQRPFASATALHGAMMDVVRKLDEPRQRDFLNLHPQLSARNIRA
GTMTADSNAEQKSAGLDAMSAQQEAALDQLNADYQARHGFPFIICVRHYTREGIFAAFERRLGRSTQQELDEALAQIAAI
TRGRLAARLAG

Specific function: Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5- ureidoimidazoline (OHCU) to (S)-allantoin [H]

COG id: COG3195

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the uricase family [H]

Homologues:

Organism=Homo sapiens, GI157743280, Length=163, Percent_Identity=36.1963190184049, Blast_Score=108, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018020
- InterPro:   IPR017580
- InterPro:   IPR002042
- InterPro:   IPR019842 [H]

Pfam domain/function: PF09349 OHCU_decarbox; PF01014 Uricase [H]

EC number: =1.7.3.3 [H]

Molecular weight: Translated: 18785; Mature: 18654

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPLDLASVNALDEAAFADAFGSVFEHFPQAAEGAWAQRPFASATALHGAMMDVVRKLD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHC
EPRQRDFLNLHPQLSARNIRAGTMTADSNAEQKSAGLDAMSAQQEAALDQLNADYQARHG
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCHHHHCC
FPFIICVRHYTREGIFAAFERRLGRSTQQELDEALAQIAAITRGRLAARLAG
CCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQPLDLASVNALDEAAFADAFGSVFEHFPQAAEGAWAQRPFASATALHGAMMDVVRKLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHCCCHHHHHHHHHHHHHHHHHHC
EPRQRDFLNLHPQLSARNIRAGTMTADSNAEQKSAGLDAMSAQQEAALDQLNADYQARHG
CHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCHHHHCC
FPFIICVRHYTREGIFAAFERRLGRSTQQELDEALAQIAAITRGRLAARLAG
CCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]