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The map label for this gene is paaF [C]

Identifier: 116694932

GI number: 116694932

Start: 1113218

End: 1113985

Strand: Direct

Name: paaF [C]

Synonym: H16_B0987

Alternate gene names: 116694932

Gene position: 1113218-1113985 (Clockwise)

Preceding gene: 116694931

Following gene: 116694933

Centisome position: 38.22

GC content: 67.06

Gene sequence:

>768_bases
ATGAATGAAGAACTCCTGTGCGAAGTCCGCGGCGCGGTCCTCTGGCTGACCATCAATCGGCCCGAGCGCCGCAACGCGAT
CAATCCGGCGGTACTGGACGGGTTGCACGCGGGGATCGACCGTGCCAACCACGATGAGGCCATCCGAGCCGTGGTCATCA
CCGGTGCCGGAGACCAGGCCTTTTGCGCGGGCGCCGACCTGCAGAGCGGCAAGTCCTTTGCGTTCGACTATTCGGAGCCG
AGCCAGCGGCTTGCCGACCTGCTGCGCTGCGCGCGGCGCTCCAACGTGCCGCTGATCGCGCGCGTCAATGGCGCCTGCAT
GGCTGGCGGCATGGGGCTGATGGCCATGTGCGACATGGCGGTGGCCAGCGAGCGGGCTGTGTTCGGCCTGCCCGAGGTCA
AGGTGGGGGTGTTCCCGGCGCAGGTGCTGAGCGTGCTGCAGCATCTGGTGCCGCGGCGCGTGCTCAATGAGCTGTGCCTC
TGCGGCGAGCCGCTGACCGCGCAGGAAGCGCTGGCGGCAGGGCTGGTCAACCACGTCAGTACGGACCTGGACGGCAAGCT
TGACTGGCTGCTGGGCCGGCTGCTCGACAAGGCGCCCGCTGCGATCCGGCGCGGGCTCTATACCCTCAAGCATATCGATG
CGATGTCCTTCGAGCAGTCGATGGCGTTCACGGAAAGCCAGATCGGCCTGTTCGCCATGACCGAGGATGCGCGCGAGGGC
CAGCTCGCCTTCCGCGAAAAGCGCAAGCCGAACTGGACCGGCAAGTAA

Upstream 100 bases:

>100_bases
ACAACGAGGCCGACATCGACAGCCTGATGGCATCCGGCGCATCGCGCGAACCGCGCCAGGCATCGGTGCATACGGCTGGC
ACGGCAAGCGAGGCCTGAAC

Downstream 100 bases:

>100_bases
GAGAAAGGCAAGGAGACAGGACCATGCATGACAAGACAGTGCGCATCGGCGGCGCTTGCGGATTCTGGGGCGACAGCAGC
GTGGGCGCGCCGCAACTGGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNEELLCEVRGAVLWLTINRPERRNAINPAVLDGLHAGIDRANHDEAIRAVVITGAGDQAFCAGADLQSGKSFAFDYSEP
SQRLADLLRCARRSNVPLIARVNGACMAGGMGLMAMCDMAVASERAVFGLPEVKVGVFPAQVLSVLQHLVPRRVLNELCL
CGEPLTAQEALAAGLVNHVSTDLDGKLDWLLGRLLDKAPAAIRRGLYTLKHIDAMSFEQSMAFTESQIGLFAMTEDAREG
QLAFREKRKPNWTGK

Sequences:

>Translated_255_residues
MNEELLCEVRGAVLWLTINRPERRNAINPAVLDGLHAGIDRANHDEAIRAVVITGAGDQAFCAGADLQSGKSFAFDYSEP
SQRLADLLRCARRSNVPLIARVNGACMAGGMGLMAMCDMAVASERAVFGLPEVKVGVFPAQVLSVLQHLVPRRVLNELCL
CGEPLTAQEALAAGLVNHVSTDLDGKLDWLLGRLLDKAPAAIRRGLYTLKHIDAMSFEQSMAFTESQIGLFAMTEDAREG
QLAFREKRKPNWTGK
>Mature_255_residues
MNEELLCEVRGAVLWLTINRPERRNAINPAVLDGLHAGIDRANHDEAIRAVVITGAGDQAFCAGADLQSGKSFAFDYSEP
SQRLADLLRCARRSNVPLIARVNGACMAGGMGLMAMCDMAVASERAVFGLPEVKVGVFPAQVLSVLQHLVPRRVLNELCL
CGEPLTAQEALAAGLVNHVSTDLDGKLDWLLGRLLDKAPAAIRRGLYTLKHIDAMSFEQSMAFTESQIGLFAMTEDAREG
QLAFREKRKPNWTGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=268, Percent_Identity=32.089552238806, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=30.4, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=30.7392996108949, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=26.7379679144385, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=26.7379679144385, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=186, Percent_Identity=30.6451612903226, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI260275230, Length=186, Percent_Identity=30.6451612903226, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI260274832, Length=186, Percent_Identity=30.6451612903226, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI116235487, Length=162, Percent_Identity=27.7777777777778, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI68989263, Length=159, Percent_Identity=30.8176100628931, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI221307494, Length=161, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=35.2713178294574, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1788597, Length=246, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=31.4606741573034, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=27.734375, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787660, Length=273, Percent_Identity=29.3040293040293, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=32.1428571428571, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=246, Percent_Identity=29.2682926829268, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=251, Percent_Identity=27.8884462151394, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=30.7086614173228, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=28.2828282828283, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=25.5639097744361, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=190, Percent_Identity=26.8421052631579, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144160, Length=259, Percent_Identity=25.0965250965251, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=259, Percent_Identity=25.0965250965251, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=182, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=181, Percent_Identity=30.939226519337, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144276, Length=179, Percent_Identity=29.0502793296089, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=179, Percent_Identity=29.0502793296089, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=179, Percent_Identity=29.0502793296089, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=219, Percent_Identity=27.8538812785388, Blast_Score=69, Evalue=6e-13,
Organism=Drosophila melanogaster, GI20129971, Length=241, Percent_Identity=29.4605809128631, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653477, Length=241, Percent_Identity=29.4605809128631, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=26.984126984127, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24654903, Length=181, Percent_Identity=30.3867403314917, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24653139, Length=262, Percent_Identity=26.7175572519084, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571730, Length=173, Percent_Identity=28.9017341040462, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571729, Length=173, Percent_Identity=28.9017341040462, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=270, Percent_Identity=27.037037037037, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583165, Length=180, Percent_Identity=28.8888888888889, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27713; Mature: 27713

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEELLCEVRGAVLWLTINRPERRNAINPAVLDGLHAGIDRANHDEAIRAVVITGAGDQA
CCCHHEEEECCEEEEEEECCCHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCC
FCAGADLQSGKSFAFDYSEPSQRLADLLRCARRSNVPLIARVNGACMAGGMGLMAMCDMA
EEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHH
VASERAVFGLPEVKVGVFPAQVLSVLQHLVPRRVLNELCLCGEPLTAQEALAAGLVNHVS
HHCCCCEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
TDLDGKLDWLLGRLLDKAPAAIRRGLYTLKHIDAMSFEQSMAFTESQIGLFAMTEDAREG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
QLAFREKRKPNWTGK
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MNEELLCEVRGAVLWLTINRPERRNAINPAVLDGLHAGIDRANHDEAIRAVVITGAGDQA
CCCHHEEEECCEEEEEEECCCHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCC
FCAGADLQSGKSFAFDYSEPSQRLADLLRCARRSNVPLIARVNGACMAGGMGLMAMCDMA
EEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHCCCHHHHHHHHH
VASERAVFGLPEVKVGVFPAQVLSVLQHLVPRRVLNELCLCGEPLTAQEALAAGLVNHVS
HHCCCCEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
TDLDGKLDWLLGRLLDKAPAAIRRGLYTLKHIDAMSFEQSMAFTESQIGLFAMTEDAREG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
QLAFREKRKPNWTGK
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA