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The map label for this gene is phnB [H]

Identifier: 116694778

GI number: 116694778

Start: 941453

End: 942757

Strand: Direct

Name: phnB [H]

Synonym: H16_B0832

Alternate gene names: 116694778

Gene position: 941453-942757 (Clockwise)

Preceding gene: 116694777

Following gene: 116694779

Centisome position: 32.32

GC content: 65.98

Gene sequence:

>1305_bases
ATGGAAACTGCCGATCGCATCCAGGCAGGGGCGTTCGTCGCTCCGGCCGAGCATTCGTTCCGGCGCGCCCAGTGGCGCAT
GCTGCTGGCCGCGATGTTCTGCTACTTCTTCTTCTACACGGGGCGGCAGACCTTTGGCTTCGCCATTCCCGGCATCCAGC
AGGAATTCGGGTTTTCCAAGGAAGCGCTGGGCTGGGCCTCCACCTGCCTGCTGTGGTGCTACGCCATCGGGCAGGCCATC
AACGGCAACCTGGGTGACAAGTTCGGCGGCCGCCGCGTGATGACCGCCGGCGCGATCCTGTCGTGCGCGGCCAACTGGGT
GGTCAGCTTCGCCGTCGGCTTCAAGAGCCTGGCGATTCCCTGGGGCATCAACGGCTACTTCCAGGCCCTTGGCTGGGCGC
CCGGCAGCCGGCTGCTGTCGAACTGGTGGGGCGCCGAAGAGCGCGGCAAAGTCTACGGCTTCTACGTGTTCGCGGCCGGC
TGCGCCTCGGTGCTGTCGTTCGTCACCTCGATCGTGGTCGTGAATATCCTGCACCTGGACTGGCGCTGGATCTTCCGCCT
GCCGGTGCTGCTGATGCTGGTGGGCGGCATCACCTTCTTCCTGATCGTGCGCGAGCGGCCGGAAGACCTTGGCTTCAAGT
CGCCCGATACCGGCGTTGCCAAGGCCGAGGGTGCGGAGAAGGCTACCGCAGTCGAGACCGATGCCGGCGAAAGCTCCTGG
TCGCGCTACAAGGCCGTGCTGCGCAACCCGCGGCTTCTGATCGCGGGCCTGTCGATCGGTTTCCAGAATGCCGCGCGCTA
TGGCCTGATCGTCTGGGTGCCGGTCCACTTCCTGGGCAAGAACTGGAAGAGCGCCGAGACCCTGATCGACCCGGCCTGGA
TCTCGGTGGCGCTGCCGGTGGGCATGGCCTTCGGCGCGCTGTCCAACGGCTGGATTTCCGACCGCCTGTTCGGCTCCAGC
CGCAGCAAGGCCATCATGCTCTACATGGTGCTGGGTGCGATTGCCTCGATGATCATGTACCAGCTGCCCACGGGCATGGG
CGCGATCGTCGCGCTGTTCCTTGCCGGCTTCTTCGTGTACGGCCCCGCCTCGTCCTTCTGGGCGCTGTGCCCGGACCTGG
TCGGCGCCAAGCGGGCCGGCACCGCCACCGGTATCCTGAACTTCTTCTCGTACCTGCTCGCAGGCCTGGGCGAACCCCTG
ATCGGCCGCATCCTGGACCAGTCCGGCAATACCTCGCTGGTGTTCCCGATCGTGGCAACCAGCTGCATCATCAGCGCCGT
GATCGCGGCCTTCATCCGCCGCTGA

Upstream 100 bases:

>100_bases
ATCGGCGCTGTGCCGGCCTAGTAGCACGACCCCCGGCCGACGGGTGCAGCCGGCCAACACGAGCCCGCAATACGGGCCGA
CAATACAAGAGGAGACAAAA

Downstream 100 bases:

>100_bases
GCGCGCCGGGCCGGCGCCTTGCCGGCCCGCCCGCGACGGCTGCATCTCCTTCCACACACTGCTCAAGACCCGAGAGGGCA
AGCTCATGACCGCATTGCAA

Product: 2-phosphonopropionate transporter

Products: Proton [Cytoplasm]; glycerol-3-phosphate [Cytoplasm] [C]

Alternate protein names: G-3-P transporter; G-3-P permease [H]

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
METADRIQAGAFVAPAEHSFRRAQWRMLLAAMFCYFFFYTGRQTFGFAIPGIQQEFGFSKEALGWASTCLLWCYAIGQAI
NGNLGDKFGGRRVMTAGAILSCAANWVVSFAVGFKSLAIPWGINGYFQALGWAPGSRLLSNWWGAEERGKVYGFYVFAAG
CASVLSFVTSIVVVNILHLDWRWIFRLPVLLMLVGGITFFLIVRERPEDLGFKSPDTGVAKAEGAEKATAVETDAGESSW
SRYKAVLRNPRLLIAGLSIGFQNAARYGLIVWVPVHFLGKNWKSAETLIDPAWISVALPVGMAFGALSNGWISDRLFGSS
RSKAIMLYMVLGAIASMIMYQLPTGMGAIVALFLAGFFVYGPASSFWALCPDLVGAKRAGTATGILNFFSYLLAGLGEPL
IGRILDQSGNTSLVFPIVATSCIISAVIAAFIRR

Sequences:

>Translated_434_residues
METADRIQAGAFVAPAEHSFRRAQWRMLLAAMFCYFFFYTGRQTFGFAIPGIQQEFGFSKEALGWASTCLLWCYAIGQAI
NGNLGDKFGGRRVMTAGAILSCAANWVVSFAVGFKSLAIPWGINGYFQALGWAPGSRLLSNWWGAEERGKVYGFYVFAAG
CASVLSFVTSIVVVNILHLDWRWIFRLPVLLMLVGGITFFLIVRERPEDLGFKSPDTGVAKAEGAEKATAVETDAGESSW
SRYKAVLRNPRLLIAGLSIGFQNAARYGLIVWVPVHFLGKNWKSAETLIDPAWISVALPVGMAFGALSNGWISDRLFGSS
RSKAIMLYMVLGAIASMIMYQLPTGMGAIVALFLAGFFVYGPASSFWALCPDLVGAKRAGTATGILNFFSYLLAGLGEPL
IGRILDQSGNTSLVFPIVATSCIISAVIAAFIRR
>Mature_434_residues
METADRIQAGAFVAPAEHSFRRAQWRMLLAAMFCYFFFYTGRQTFGFAIPGIQQEFGFSKEALGWASTCLLWCYAIGQAI
NGNLGDKFGGRRVMTAGAILSCAANWVVSFAVGFKSLAIPWGINGYFQALGWAPGSRLLSNWWGAEERGKVYGFYVFAAG
CASVLSFVTSIVVVNILHLDWRWIFRLPVLLMLVGGITFFLIVRERPEDLGFKSPDTGVAKAEGAEKATAVETDAGESSW
SRYKAVLRNPRLLIAGLSIGFQNAARYGLIVWVPVHFLGKNWKSAETLIDPAWISVALPVGMAFGALSNGWISDRLFGSS
RSKAIMLYMVLGAIASMIMYQLPTGMGAIVALFLAGFFVYGPASSFWALCPDLVGAKRAGTATGILNFFSYLLAGLGEPL
IGRILDQSGNTSLVFPIVATSCIISAVIAAFIRR

Specific function: Responsible for glycerol-3-phosphate uptake [H]

COG id: COG2271

COG function: function code G; Sugar phosphate permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family [H]

Homologues:

Organism=Homo sapiens, GI256219598, Length=387, Percent_Identity=24.031007751938, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI4503847, Length=387, Percent_Identity=24.031007751938, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI256219483, Length=387, Percent_Identity=24.031007751938, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI256219543, Length=325, Percent_Identity=24.3076923076923, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI46361976, Length=411, Percent_Identity=26.0340632603406, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI223717983, Length=423, Percent_Identity=24.113475177305, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI38093649, Length=423, Percent_Identity=24.113475177305, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788573, Length=396, Percent_Identity=25.7575757575758, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87082310, Length=419, Percent_Identity=25.2983293556086, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082320, Length=404, Percent_Identity=25, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI2367379, Length=429, Percent_Identity=23.7762237762238, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI133901856, Length=346, Percent_Identity=26.3005780346821, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI133901854, Length=346, Percent_Identity=26.3005780346821, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI133930953, Length=452, Percent_Identity=22.1238938053097, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17542316, Length=396, Percent_Identity=23.4848484848485, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005267
- InterPro:   IPR000849
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR021159 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47123; Mature: 47123

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METADRIQAGAFVAPAEHSFRRAQWRMLLAAMFCYFFFYTGRQTFGFAIPGIQQEFGFSK
CCCHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCCH
EALGWASTCLLWCYAIGQAINGNLGDKFGGRRVMTAGAILSCAANWVVSFAVGFKSLAIP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEECC
WGINGYFQALGWAPGSRLLSNWWGAEERGKVYGFYVFAAGCASVLSFVTSIVVVNILHLD
CCCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
WRWIFRLPVLLMLVGGITFFLIVRERPEDLGFKSPDTGVAKAEGAEKATAVETDAGESSW
HHHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCCCCCCCCCCCCCCHHHEEECCCCCHHH
SRYKAVLRNPRLLIAGLSIGFQNAARYGLIVWVPVHFLGKNWKSAETLIDPAWISVALPV
HHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEEHHHHCCCCCCHHHHCCHHHHHHHHHH
GMAFGALSNGWISDRLFGSSRSKAIMLYMVLGAIASMIMYQLPTGMGAIVALFLAGFFVY
HHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GPASSFWALCPDLVGAKRAGTATGILNFFSYLLAGLGEPLIGRILDQSGNTSLVFPIVAT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEHHHHHH
SCIISAVIAAFIRR
HHHHHHHHHHHHCC
>Mature Secondary Structure
METADRIQAGAFVAPAEHSFRRAQWRMLLAAMFCYFFFYTGRQTFGFAIPGIQQEFGFSK
CCCHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCCH
EALGWASTCLLWCYAIGQAINGNLGDKFGGRRVMTAGAILSCAANWVVSFAVGFKSLAIP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEECC
WGINGYFQALGWAPGSRLLSNWWGAEERGKVYGFYVFAAGCASVLSFVTSIVVVNILHLD
CCCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
WRWIFRLPVLLMLVGGITFFLIVRERPEDLGFKSPDTGVAKAEGAEKATAVETDAGESSW
HHHHHHHHHHHHHHHHHHEEEEEECCCHHCCCCCCCCCCCCCCCCCHHHEEECCCCCHHH
SRYKAVLRNPRLLIAGLSIGFQNAARYGLIVWVPVHFLGKNWKSAETLIDPAWISVALPV
HHHHHHHCCCCEEEEEECCCHHHHCCCCEEEEEEHHHHCCCCCCHHHHCCHHHHHHHHHH
GMAFGALSNGWISDRLFGSSRSKAIMLYMVLGAIASMIMYQLPTGMGAIVALFLAGFFVY
HHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GPASSFWALCPDLVGAKRAGTATGILNFFSYLLAGLGEPLIGRILDQSGNTSLVFPIVAT
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEHHHHHH
SCIISAVIAAFIRR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; glycerol-3-phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + glycerol-3-phosphate [Periplasm] = Proton [Cytoplasm] + glycerol-3-phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8012593; 9384377 [H]