Click here to switch to the map view.

The map label for this gene is menE [H]

Identifier: 116694704

GI number: 116694704

Start: 849422

End: 850969

Strand: Direct

Name: menE [H]

Synonym: H16_B0753

Alternate gene names: 116694704

Gene position: 849422-850969 (Clockwise)

Preceding gene: 116694699

Following gene: 116694705

Centisome position: 29.16

GC content: 67.64

Gene sequence:

>1548_bases
ATGCATCCGCACATCCACGCGCAGCGCACACCCGAAAAACCAGCCGTCATCATGGGTGGCAGCGGCGCAGTCGTCACCTA
CCGCGAACTGGACGAACGCTCGAACCAGGTCGCCCACCTGTTCCGCAGCCAGGGACTGCAGCCCGGTGACCGCGTGGCGT
TCATGGTCGAGAATCATCCGCGCCTGTTCGAACTGTGCTGGGGCGCGCAGCGCAGCGGCATTGTCTATGTCTGCCTGAGC
ACGCGGCTGAACGTGGCCGATGCCGCCCACATCATCAATGACAGCGGCGCCAGGCTGCTGGTCACCACGCACGCGCAGGC
CGAGGTCGCGGCAGCCCTGGCCGGGCAGACGCCGGCCTTGCGCGGCAGGCTGATGCTGGACGGCACAATGCCGGGCTATG
ACGCGTACGAGACCGCGCTGGCGCGCTGCCCGGCAACGCGCATCGATGACGAAGTGACCGGCGGCGACATGCTGTATTCG
TCCGGCACCACGGGCCGGCCCAAGGGAGTGTATGCGCCGCCATCAAGCCCGAATATCGACGATCCCACGACCCTGACCAG
CCTGTGCCAGCGGCTCTATGGCTTCGATGCCGAGACGCGCTACCTGTCGCCCGCGCCGCTGTACCACGCGGCGCCGCTGC
GCTACAACATGACCGTGCAGGCGCTGGGCGGGACCGCGGTGGTGATGGAGCATTTCGATGCCGAGCACTATCTGCAGCTG
GTGCAGCAGCACCGCATCACGCATACGCAGCTGGTACCGACGATGTTCTCGCGCATGCTCAAGCTGCCCGAGGCGCAGCG
CCAGGCCTATGATGTGTCGTCGCTGCGCGTGGCGATCCACGCGGCCGCGCCGTGCCCGGTGCAGGTCAAGGAAGCCATGA
TCGCGTGGTGGGGGCCGGTGATCTGGGAGTACTACGCCGGGACGGAGGGCAACGGCGTGACCGTGGTCAGCACGCCGGAG
TGGCTGGAGCGCAAGGGCACGGTGGGCCGGGCCATGGTCGGCAAGCTGCGCATCTGCGGGCCGGACGGCGCGCTGCTGCC
GCCGGGCGAATCGGGCACGATCTACTTTGCCGAAGGCCGCGACTTTTCGTACCACAACGACGAGGCCAAGACCGCGGAGT
CGCGCCACCCGCAGCAGCCGGACTGGAGCACCATCGGCGATGTCGGCTACGTGGATGCGGACGGCTACCTGTACCTGACC
GACCGCAAGGCCAACATGATCATCTCCGGCGGCGTCAACATCTACCCGCAGGAGGCCGAGAACCTGCTGATGACGCATCC
CAAGGTGATGGACGTGGCCGTGATCGGCGTGCCCAACGAGGACTTCGGCGAAGAGGTCAAGGCCGTGGTGCAGCCCGTCG
ACATGAGCCAGGCCGGTCCCGAGCTGGCCGCGGAACTGATCGCGTTCTGCCGCGCCAACCTGTCGGCCATCAAGTGCCCG
CGCTCGGTCGACTTCGCGTCCGAGCTGCCGCGCCTGCCGACCGGTAAGCTGCTCAAGCGGCTGCTGCGCGACCGCTACTG
GGGCGGCCACGCCAACAAGCTGGTGTAG

Upstream 100 bases:

>100_bases
GCCGCTTCGTCGCTTCCGACGATGGTTGAACCGGCTGGGGTTCCGAGAATGCGGTACCGGCATCCGCCAACACACCGACA
CCCTCGAGGAGTCCAACGCC

Downstream 100 bases:

>100_bases
GGCCGTCTGCCGCTGCCCGCATTTTCGCGAGTGCGGCGGTTTACGTCATAATCCTCCGTTACATCACAGCCACGGCCCGC
CTGCCTGCATCCGCAGCGCG

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MHPHIHAQRTPEKPAVIMGGSGAVVTYRELDERSNQVAHLFRSQGLQPGDRVAFMVENHPRLFELCWGAQRSGIVYVCLS
TRLNVADAAHIINDSGARLLVTTHAQAEVAAALAGQTPALRGRLMLDGTMPGYDAYETALARCPATRIDDEVTGGDMLYS
SGTTGRPKGVYAPPSSPNIDDPTTLTSLCQRLYGFDAETRYLSPAPLYHAAPLRYNMTVQALGGTAVVMEHFDAEHYLQL
VQQHRITHTQLVPTMFSRMLKLPEAQRQAYDVSSLRVAIHAAAPCPVQVKEAMIAWWGPVIWEYYAGTEGNGVTVVSTPE
WLERKGTVGRAMVGKLRICGPDGALLPPGESGTIYFAEGRDFSYHNDEAKTAESRHPQQPDWSTIGDVGYVDADGYLYLT
DRKANMIISGGVNIYPQEAENLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPVDMSQAGPELAAELIAFCRANLSAIKCP
RSVDFASELPRLPTGKLLKRLLRDRYWGGHANKLV

Sequences:

>Translated_515_residues
MHPHIHAQRTPEKPAVIMGGSGAVVTYRELDERSNQVAHLFRSQGLQPGDRVAFMVENHPRLFELCWGAQRSGIVYVCLS
TRLNVADAAHIINDSGARLLVTTHAQAEVAAALAGQTPALRGRLMLDGTMPGYDAYETALARCPATRIDDEVTGGDMLYS
SGTTGRPKGVYAPPSSPNIDDPTTLTSLCQRLYGFDAETRYLSPAPLYHAAPLRYNMTVQALGGTAVVMEHFDAEHYLQL
VQQHRITHTQLVPTMFSRMLKLPEAQRQAYDVSSLRVAIHAAAPCPVQVKEAMIAWWGPVIWEYYAGTEGNGVTVVSTPE
WLERKGTVGRAMVGKLRICGPDGALLPPGESGTIYFAEGRDFSYHNDEAKTAESRHPQQPDWSTIGDVGYVDADGYLYLT
DRKANMIISGGVNIYPQEAENLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPVDMSQAGPELAAELIAFCRANLSAIKCP
RSVDFASELPRLPTGKLLKRLLRDRYWGGHANKLV
>Mature_515_residues
MHPHIHAQRTPEKPAVIMGGSGAVVTYRELDERSNQVAHLFRSQGLQPGDRVAFMVENHPRLFELCWGAQRSGIVYVCLS
TRLNVADAAHIINDSGARLLVTTHAQAEVAAALAGQTPALRGRLMLDGTMPGYDAYETALARCPATRIDDEVTGGDMLYS
SGTTGRPKGVYAPPSSPNIDDPTTLTSLCQRLYGFDAETRYLSPAPLYHAAPLRYNMTVQALGGTAVVMEHFDAEHYLQL
VQQHRITHTQLVPTMFSRMLKLPEAQRQAYDVSSLRVAIHAAAPCPVQVKEAMIAWWGPVIWEYYAGTEGNGVTVVSTPE
WLERKGTVGRAMVGKLRICGPDGALLPPGESGTIYFAEGRDFSYHNDEAKTAESRHPQQPDWSTIGDVGYVDADGYLYLT
DRKANMIISGGVNIYPQEAENLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPVDMSQAGPELAAELIAFCRANLSAIKCP
RSVDFASELPRLPTGKLLKRLLRDRYWGGHANKLV

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=538, Percent_Identity=26.7657992565056, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI38505220, Length=526, Percent_Identity=27.7566539923954, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI122937307, Length=543, Percent_Identity=27.8084714548803, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI115511026, Length=537, Percent_Identity=26.0707635009311, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI42544132, Length=535, Percent_Identity=25.4205607476636, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI157311624, Length=537, Percent_Identity=25.1396648044693, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI157311622, Length=537, Percent_Identity=25.1396648044693, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI58082049, Length=519, Percent_Identity=26.0115606936416, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI28416953, Length=571, Percent_Identity=22.5919439579685, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI38524616, Length=484, Percent_Identity=25.8264462809917, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI40807357, Length=547, Percent_Identity=25.7769652650823, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI62865631, Length=540, Percent_Identity=22.4074074074074, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI13325055, Length=540, Percent_Identity=22.4074074074074, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI227499619, Length=472, Percent_Identity=22.6694915254237, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI221142682, Length=521, Percent_Identity=28.0230326295585, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI1788107, Length=550, Percent_Identity=27.6363636363636, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI145693145, Length=512, Percent_Identity=27.9296875, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790505, Length=548, Percent_Identity=21.8978102189781, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1786529, Length=557, Percent_Identity=26.032315978456, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786810, Length=513, Percent_Identity=25.3411306042885, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=30.2970297029703, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI32563687, Length=507, Percent_Identity=28.9940828402367, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531443, Length=529, Percent_Identity=24.5746691871456, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI71994694, Length=542, Percent_Identity=26.7527675276753, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=542, Percent_Identity=26.7527675276753, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=542, Percent_Identity=26.7527675276753, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=507, Percent_Identity=26.0355029585799, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=24.7672253258845, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17559526, Length=508, Percent_Identity=25.3937007874016, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17538037, Length=499, Percent_Identity=24.8496993987976, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=505, Percent_Identity=21.3861386138614, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=24.7933884297521, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=361, Percent_Identity=27.4238227146814, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319264, Length=577, Percent_Identity=21.4904679376083, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355181, Length=488, Percent_Identity=28.0737704918033, Blast_Score=149, Evalue=5e-36,
Organism=Drosophila melanogaster, GI24648253, Length=471, Percent_Identity=24.6284501061571, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648255, Length=471, Percent_Identity=24.6284501061571, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24581924, Length=533, Percent_Identity=24.202626641651, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI21356441, Length=503, Percent_Identity=23.6580516898608, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI18859661, Length=506, Percent_Identity=25.4940711462451, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24656500, Length=505, Percent_Identity=23.7623762376238, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24648257, Length=518, Percent_Identity=24.1312741312741, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24648260, Length=524, Percent_Identity=24.4274809160305, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24653035, Length=510, Percent_Identity=24.1176470588235, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI161076582, Length=517, Percent_Identity=24.3713733075435, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281365686, Length=327, Percent_Identity=25.9938837920489, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358303, Length=467, Percent_Identity=23.1263383297645, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI20130357, Length=516, Percent_Identity=23.062015503876, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI62472339, Length=552, Percent_Identity=22.1014492753623, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24667955, Length=552, Percent_Identity=22.1014492753623, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI45552801, Length=531, Percent_Identity=23.728813559322, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45550496, Length=531, Percent_Identity=23.728813559322, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19922652, Length=507, Percent_Identity=25.4437869822485, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356947, Length=507, Percent_Identity=23.8658777120316, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667959, Length=376, Percent_Identity=24.2021276595745, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355225, Length=372, Percent_Identity=24.1935483870968, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 56389; Mature: 56389

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHPHIHAQRTPEKPAVIMGGSGAVVTYRELDERSNQVAHLFRSQGLQPGDRVAFMVENHP
CCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
RLFELCWGAQRSGIVYVCLSTRLNVADAAHIINDSGARLLVTTHAQAEVAAALAGQTPAL
CHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCC
RGRLMLDGTMPGYDAYETALARCPATRIDDEVTGGDMLYSSGTTGRPKGVYAPPSSPNID
CCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCC
DPTTLTSLCQRLYGFDAETRYLSPAPLYHAAPLRYNMTVQALGGTAVVMEHFDAEHYLQL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECEEEEECCCEEEEHHHCCHHHHHHH
VQQHRITHTQLVPTMFSRMLKLPEAQRQAYDVSSLRVAIHAAAPCPVQVKEAMIAWWGPV
HHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
IWEYYAGTEGNGVTVVSTPEWLERKGTVGRAMVGKLRICGPDGALLPPGESGTIYFAEGR
HHHHHCCCCCCCEEEEECHHHHHHCCCCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCC
DFSYHNDEAKTAESRHPQQPDWSTIGDVGYVDADGYLYLTDRKANMIISGGVNIYPQEAE
CCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEEECCCEECCCCCC
NLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPVDMSQAGPELAAELIAFCRANLSAIKCP
HHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
RSVDFASELPRLPTGKLLKRLLRDRYWGGHANKLV
CCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MHPHIHAQRTPEKPAVIMGGSGAVVTYRELDERSNQVAHLFRSQGLQPGDRVAFMVENHP
CCCCCCCCCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCC
RLFELCWGAQRSGIVYVCLSTRLNVADAAHIINDSGARLLVTTHAQAEVAAALAGQTPAL
CHHHHHCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCEEEEEECCHHHHHHHHHCCCCCC
RGRLMLDGTMPGYDAYETALARCPATRIDDEVTGGDMLYSSGTTGRPKGVYAPPSSPNID
CCEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCC
DPTTLTSLCQRLYGFDAETRYLSPAPLYHAAPLRYNMTVQALGGTAVVMEHFDAEHYLQL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECEEEEECCCEEEEHHHCCHHHHHHH
VQQHRITHTQLVPTMFSRMLKLPEAQRQAYDVSSLRVAIHAAAPCPVQVKEAMIAWWGPV
HHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHH
IWEYYAGTEGNGVTVVSTPEWLERKGTVGRAMVGKLRICGPDGALLPPGESGTIYFAEGR
HHHHHCCCCCCCEEEEECHHHHHHCCCCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCC
DFSYHNDEAKTAESRHPQQPDWSTIGDVGYVDADGYLYLTDRKANMIISGGVNIYPQEAE
CCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEEECCCEECCCCCC
NLLMTHPKVMDVAVIGVPNEDFGEEVKAVVQPVDMSQAGPELAAELIAFCRANLSAIKCP
HHHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHCCC
RSVDFASELPRLPTGKLLKRLLRDRYWGGHANKLV
CCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA