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The map label for this gene is fadE12 [H]

Identifier: 116694616

GI number: 116694616

Start: 739653

End: 740855

Strand: Direct

Name: fadE12 [H]

Synonym: H16_B0665

Alternate gene names: 116694616

Gene position: 739653-740855 (Clockwise)

Preceding gene: 116694615

Following gene: 116694617

Centisome position: 25.4

GC content: 71.24

Gene sequence:

>1203_bases
ATGACAGCGAATCAAGAGGCGGCATTCCGCGCCGAAGTCGCGCAGTGGATGGCCGCCAACCTGGCCGGCGAGTTCGCCTG
CCTGAAGCACCGCGGCGGCCCCGGCGACGAAGAGGCCTATCCCGAACTGCGCAAGGCGTGGGAGCGCCGGCTGGCCGAAG
GCGGCTGGACCGGCCTGGGCTGGCCGCGCGCGCATGGCGGGCGCGAGCTGCCGGTCATGCAGCAGGTGATCTTCCATGAG
GAATACGCGCGGGCAGGGGGGCCGGGCCGCATGGGCCATATCGGCGAGGGGCTGATCGGCCCGACCCTGGTCGCCTTCGG
CACCGACGACCAGAAGGCGCGCTTGCTGCCCGGCATCCTGAACGGCACCACGTTCTGGTGCCAGGGGTATTCGGAGCCTG
GCGCGGGCTCGGACCTGGCCAATGTGCGCACGCGGGCGGAGCGCGATGCGGCCACCGGCGACTGGCTGGTGAGCGGCCAG
AAGGTGTGGACCTCGCTCGCGCACGATTCGGACTGGATCTTCGTGCTGGCGCGCTGCGAGCCCGGCTCGCGCGGCAGCAA
GGGCCTGGTCTTCCTGATGCTGCCGCTGGACCAGCCCGGCATCGAGATCCGGCCGATTCGCCAGATGGGCGGCGGCGCCG
AATTCAACGAGGTGTTCTTCGACGGCGCACGTGCCGCCGCCGCCGATGTGCTGGGCGCGCCCGGCGATGGCTGGCGCATC
GCCATGGCGCTGCTGGGCTTCGAGCGCGGCATCTCCACGCTGGGCCAGCAGATGCAGTTCACGCACGAACTGGAATGGGT
GGCGCAGGCCGCGCGCGACAACGGCAGCAGCCGCGACGCGCTGGTGCGCCAGCGCATCGCGCGCGCCTGGGCCGGCCTGC
GCGTGATGCGCGCCAATGCGCTGCGCATGCTGGCGGGCGCGGCGCAGGCGGGCCAGGACGGCGGCACCGCGCTGCAGCGC
GACGCGCTGATCTACAAGTACTACTGGTCCAACTGGCACCGAGACCTGGGCCAGCTGGCGCTGGACGTGCTGGGTCCGCT
GGCCAATGTGCTGGACGCCGGCGACACGCGCCGCACGCGGCTGCAGCAGATGGCGCTGTTCTCGCGCGCCGACACCATCT
ACGCCGGCACCAACGAAATCCAGCTCAACATCATCGCCGAACGCGGCCTCGGCATGCCGCGCGAAGCACGAGGACAGTCA
TGA

Upstream 100 bases:

>100_bases
TCTATTTCAAGCGCGCGCAGGCCGCCAGCCACTGGCTGGGCGGTGCCGGCGCGGCGCTGGCGTATCTCGCAGCAAATGGC
CCGAACGCGTGGAGGACAGC

Downstream 100 bases:

>100_bases
CCCTGAACACCGAAATCCCCAGCGGCCCGGCCTATGTGCCTGGCCATCAGTTGCTCGCCGGCAAGAGTGTGCTCATCACC
GCCGCGGCGGGCGCCGGCAT

Product: Acyl-CoA dehydrogenase

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTANQEAAFRAEVAQWMAANLAGEFACLKHRGGPGDEEAYPELRKAWERRLAEGGWTGLGWPRAHGGRELPVMQQVIFHE
EYARAGGPGRMGHIGEGLIGPTLVAFGTDDQKARLLPGILNGTTFWCQGYSEPGAGSDLANVRTRAERDAATGDWLVSGQ
KVWTSLAHDSDWIFVLARCEPGSRGSKGLVFLMLPLDQPGIEIRPIRQMGGGAEFNEVFFDGARAAAADVLGAPGDGWRI
AMALLGFERGISTLGQQMQFTHELEWVAQAARDNGSSRDALVRQRIARAWAGLRVMRANALRMLAGAAQAGQDGGTALQR
DALIYKYYWSNWHRDLGQLALDVLGPLANVLDAGDTRRTRLQQMALFSRADTIYAGTNEIQLNIIAERGLGMPREARGQS

Sequences:

>Translated_400_residues
MTANQEAAFRAEVAQWMAANLAGEFACLKHRGGPGDEEAYPELRKAWERRLAEGGWTGLGWPRAHGGRELPVMQQVIFHE
EYARAGGPGRMGHIGEGLIGPTLVAFGTDDQKARLLPGILNGTTFWCQGYSEPGAGSDLANVRTRAERDAATGDWLVSGQ
KVWTSLAHDSDWIFVLARCEPGSRGSKGLVFLMLPLDQPGIEIRPIRQMGGGAEFNEVFFDGARAAAADVLGAPGDGWRI
AMALLGFERGISTLGQQMQFTHELEWVAQAARDNGSSRDALVRQRIARAWAGLRVMRANALRMLAGAAQAGQDGGTALQR
DALIYKYYWSNWHRDLGQLALDVLGPLANVLDAGDTRRTRLQQMALFSRADTIYAGTNEIQLNIIAERGLGMPREARGQS
>Mature_399_residues
TANQEAAFRAEVAQWMAANLAGEFACLKHRGGPGDEEAYPELRKAWERRLAEGGWTGLGWPRAHGGRELPVMQQVIFHEE
YARAGGPGRMGHIGEGLIGPTLVAFGTDDQKARLLPGILNGTTFWCQGYSEPGAGSDLANVRTRAERDAATGDWLVSGQK
VWTSLAHDSDWIFVLARCEPGSRGSKGLVFLMLPLDQPGIEIRPIRQMGGGAEFNEVFFDGARAAAADVLGAPGDGWRIA
MALLGFERGISTLGQQMQFTHELEWVAQAARDNGSSRDALVRQRIARAWAGLRVMRANALRMLAGAAQAGQDGGTALQRD
ALIYKYYWSNWHRDLGQLALDVLGPLANVLDAGDTRRTRLQQMALFSRADTIYAGTNEIQLNIIAERGLGMPREARGQS

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4557231, Length=356, Percent_Identity=25.2808988764045, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI187960098, Length=356, Percent_Identity=25.2808988764045, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI4501859, Length=360, Percent_Identity=26.3888888888889, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI226958412, Length=214, Percent_Identity=27.1028037383178, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI226958414, Length=212, Percent_Identity=26.8867924528302, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI4557233, Length=339, Percent_Identity=25.6637168141593, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI76496475, Length=380, Percent_Identity=25.7894736842105, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4557235, Length=380, Percent_Identity=25.7894736842105, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4501857, Length=353, Percent_Identity=24.929178470255, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI21361497, Length=370, Percent_Identity=25.6756756756757, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786223, Length=347, Percent_Identity=23.6311239193084, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081958, Length=391, Percent_Identity=24.0409207161125, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17570075, Length=401, Percent_Identity=24.4389027431421, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17508101, Length=342, Percent_Identity=26.0233918128655, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17538396, Length=250, Percent_Identity=26, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17569725, Length=193, Percent_Identity=28.4974093264249, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506239, Length=365, Percent_Identity=24.9315068493151, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17505929, Length=265, Percent_Identity=24.1509433962264, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17551932, Length=155, Percent_Identity=28.3870967741935, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71990804, Length=196, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17534899, Length=200, Percent_Identity=25.5, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71985184, Length=145, Percent_Identity=33.1034482758621, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71985192, Length=146, Percent_Identity=32.8767123287671, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24660351, Length=211, Percent_Identity=31.7535545023697, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24666513, Length=360, Percent_Identity=23.3333333333333, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356377, Length=363, Percent_Identity=26.9972451790634, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281363737, Length=299, Percent_Identity=23.7458193979933, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21355753, Length=217, Percent_Identity=27.6497695852535, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24646207, Length=357, Percent_Identity=26.890756302521, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 43553; Mature: 43422

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANQEAAFRAEVAQWMAANLAGEFACLKHRGGPGDEEAYPELRKAWERRLAEGGWTGLG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
WPRAHGGRELPVMQQVIFHEEYARAGGPGRMGHIGEGLIGPTLVAFGTDDQKARLLPGIL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCHHH
NGTTFWCQGYSEPGAGSDLANVRTRAERDAATGDWLVSGQKVWTSLAHDSDWIFVLARCE
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCEEEEEEECC
PGSRGSKGLVFLMLPLDQPGIEIRPIRQMGGGAEFNEVFFDGARAAAADVLGAPGDGWRI
CCCCCCCCEEEEEEECCCCCCEEEEHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHH
AMALLGFERGISTLGQQMQFTHELEWVAQAARDNGSSRDALVRQRIARAWAGLRVMRANA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRMLAGAAQAGQDGGTALQRDALIYKYYWSNWHRDLGQLALDVLGPLANVLDAGDTRRTR
HHHHHHHHHCCCCCCCHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LQQMALFSRADTIYAGTNEIQLNIIAERGLGMPREARGQS
HHHHHHHHHCCEEEECCCEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TANQEAAFRAEVAQWMAANLAGEFACLKHRGGPGDEEAYPELRKAWERRLAEGGWTGLG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
WPRAHGGRELPVMQQVIFHEEYARAGGPGRMGHIGEGLIGPTLVAFGTDDQKARLLPGIL
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHCCHHH
NGTTFWCQGYSEPGAGSDLANVRTRAERDAATGDWLVSGQKVWTSLAHDSDWIFVLARCE
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEECCHHHHHHHCCCCCEEEEEEECC
PGSRGSKGLVFLMLPLDQPGIEIRPIRQMGGGAEFNEVFFDGARAAAADVLGAPGDGWRI
CCCCCCCCEEEEEEECCCCCCEEEEHHHCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHH
AMALLGFERGISTLGQQMQFTHELEWVAQAARDNGSSRDALVRQRIARAWAGLRVMRANA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LRMLAGAAQAGQDGGTALQRDALIYKYYWSNWHRDLGQLALDVLGPLANVLDAGDTRRTR
HHHHHHHHHCCCCCCCHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LQQMALFSRADTIYAGTNEIQLNIIAERGLGMPREARGQS
HHHHHHHHHCCEEEECCCEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]