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The map label for this gene is bphC [H]

Identifier: 116694605

GI number: 116694605

Start: 728767

End: 729663

Strand: Direct

Name: bphC [H]

Synonym: H16_B0654

Alternate gene names: 116694605

Gene position: 728767-729663 (Clockwise)

Preceding gene: 116694604

Following gene: 116694606

Centisome position: 25.02

GC content: 66.89

Gene sequence:

>897_bases
ATGTTGGATATCCGTGCGCTTGGCTACATCGTGGTCGAGTCCACCGACCTGGGGCAATGGCGGCGCTATGCCGAACAGGT
GCTGGGCATGACCTGTTCCGGCGCCCCCGGCGGTGCGCTGTACCTGAAGATGGATGAGCGCGATTACCGCTACCTGGTGG
TGCCGGGCGCGCGCGACCGCTACCTGGCATCGGGCTGGGAGCTTGCCGACGAGCCGGCCTACCGCCATGCGCTCGACACG
CTGCGCCAGGCGAACGTTGAGGTCGTGCACGCCAGCGCCGCGGAACTGGCACAGCGCCGCGTGCAGGCCATGGCCTGGTT
TGCCGATCCCTCGGGCAACCGCCATGAGATCTCCTGGGGCATGCGCTCGGACTTCCTGCGCTTTATCTCGCCGGTGGGCG
TGCCGCGCTTCGTCACTGACCCGATGGGGGCGGGCCATGCGGTCCTGCCCGCGCCGGCCTTCGACGAGACCTATGCCTTC
CTGTGCGAGGTGATGGGTTTCGGTTTGTCCGACATCTTCCGCGTGCGCTTTACCAATGACCCGGCCGAATCGGAAAAGCG
CATCCACTTCCTGCATTGCGGCAACGCCCGCCACCACAGCCTGGCGATCTTCGAGATGCCGTCGGAAGCGGGCTGCATCC
ACGTGATGGCCGAGGTTCCGGACATGGCCGAGGTGGGCCGCGCGCTGGACCGCGCGCAGCAGCACGGCGTGAAGCTCTCG
GCCACGCTGGGCCAGCACTGCAATGACCGCATGACTTCCTTCTACATGAAGACCCCCGGCGGCTTCGACCTCGAGTTCGG
CCACGGCGGCCTGGAGGTGGACTGGAGCCACCACGCCGCCTACGAGGCCACGCGCGTCAGCCTGTGGGGACACGACTTCA
GCATCGGATACCGCTAA

Upstream 100 bases:

>100_bases
GCGCCGGACCGGCTAGTCCCACTGGACGATGTGCCGGGCTCCAGCGCACGGAAGAATGGTCCCATCAGCCAGCAGTGGCA
ACGAACCTGAGGAGACCGGC

Downstream 100 bases:

>100_bases
ACACATCATGACCAAGACAATCGACAAGATCATGAGCGCGGCCGACGTGGTCGGCCAGCTCGCCGACGGCATGACCATCG
GCATCGGTGGGTGGGGCCCG

Product: biphenyl-2,3-diol 1,2-dioxygenase

Products: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate; H2O

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLDIRALGYIVVESTDLGQWRRYAEQVLGMTCSGAPGGALYLKMDERDYRYLVVPGARDRYLASGWELADEPAYRHALDT
LRQANVEVVHASAAELAQRRVQAMAWFADPSGNRHEISWGMRSDFLRFISPVGVPRFVTDPMGAGHAVLPAPAFDETYAF
LCEVMGFGLSDIFRVRFTNDPAESEKRIHFLHCGNARHHSLAIFEMPSEAGCIHVMAEVPDMAEVGRALDRAQQHGVKLS
ATLGQHCNDRMTSFYMKTPGGFDLEFGHGGLEVDWSHHAAYEATRVSLWGHDFSIGYR

Sequences:

>Translated_298_residues
MLDIRALGYIVVESTDLGQWRRYAEQVLGMTCSGAPGGALYLKMDERDYRYLVVPGARDRYLASGWELADEPAYRHALDT
LRQANVEVVHASAAELAQRRVQAMAWFADPSGNRHEISWGMRSDFLRFISPVGVPRFVTDPMGAGHAVLPAPAFDETYAF
LCEVMGFGLSDIFRVRFTNDPAESEKRIHFLHCGNARHHSLAIFEMPSEAGCIHVMAEVPDMAEVGRALDRAQQHGVKLS
ATLGQHCNDRMTSFYMKTPGGFDLEFGHGGLEVDWSHHAAYEATRVSLWGHDFSIGYR
>Mature_298_residues
MLDIRALGYIVVESTDLGQWRRYAEQVLGMTCSGAPGGALYLKMDERDYRYLVVPGARDRYLASGWELADEPAYRHALDT
LRQANVEVVHASAAELAQRRVQAMAWFADPSGNRHEISWGMRSDFLRFISPVGVPRFVTDPMGAGHAVLPAPAFDETYAF
LCEVMGFGLSDIFRVRFTNDPAESEKRIHFLHCGNARHHSLAIFEMPSEAGCIHVMAEVPDMAEVGRALDRAQQHGVKLS
ATLGQHCNDRMTSFYMKTPGGFDLEFGHGGLEVDWSHHAAYEATRVSLWGHDFSIGYR

Specific function: Catalyzes the meta-cleavage of 3,4-dihydroxy-9,10- seconandrost-1,3,5(10)-triene-9,17-dione (3,4-DHSA) to produce 4,5-9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4- oic acid (4,9-DSHA) [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR000486 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: 1.13.11.39

Molecular weight: Translated: 33309; Mature: 33309

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIRALGYIVVESTDLGQWRRYAEQVLGMTCSGAPGGALYLKMDERDYRYLVVPGARDR
CCCEEEEEEEEEECCCHHHHHHHHHHHHCEECCCCCCCEEEEEECCCCEEEEEECCCCCC
YLASGWELADEPAYRHALDTLRQANVEVVHASAAELAQRRVQAMAWFADPSGNRHEISWG
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHEEEECCCCCCEEEECC
MRSDFLRFISPVGVPRFVTDPMGAGHAVLPAPAFDETYAFLCEVMGFGLSDIFRVRFTND
HHHHHHHHHCCCCCCCEECCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCC
PAESEKRIHFLHCGNARHHSLAIFEMPSEAGCIHVMAEVPDMAEVGRALDRAQQHGVKLS
CCCCCCEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEE
ATLGQHCNDRMTSFYMKTPGGFDLEFGHGGLEVDWSHHAAYEATRVSLWGHDFSIGYR
EHHHHCCCCHHHEEEEECCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCEECCCC
>Mature Secondary Structure
MLDIRALGYIVVESTDLGQWRRYAEQVLGMTCSGAPGGALYLKMDERDYRYLVVPGARDR
CCCEEEEEEEEEECCCHHHHHHHHHHHHCEECCCCCCCEEEEEECCCCEEEEEECCCCCC
YLASGWELADEPAYRHALDTLRQANVEVVHASAAELAQRRVQAMAWFADPSGNRHEISWG
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHEEEECCCCCCEEEECC
MRSDFLRFISPVGVPRFVTDPMGAGHAVLPAPAFDETYAFLCEVMGFGLSDIFRVRFTND
HHHHHHHHHCCCCCCCEECCCCCCCCEECCCCCCHHHHHHHHHHHCCCCCCEEEEEECCC
PAESEKRIHFLHCGNARHHSLAIFEMPSEAGCIHVMAEVPDMAEVGRALDRAQQHGVKLS
CCCCCCEEEEEEECCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCCEEEE
ATLGQHCNDRMTSFYMKTPGGFDLEFGHGGLEVDWSHHAAYEATRVSLWGHDFSIGYR
EHHHHCCCCHHHEEEEECCCCCEEEECCCCEEEECCCCCCCEEEEEEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: biphenyl-2,3-diol; O2

Specific reaction: biphenyl-2,3-diol + O2 = 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]