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The map label for this gene is acrA8 [H]

Identifier: 116694533

GI number: 116694533

Start: 653028

End: 654227

Strand: Direct

Name: acrA8 [H]

Synonym: H16_B0582

Alternate gene names: 116694533

Gene position: 653028-654227 (Clockwise)

Preceding gene: 116694532

Following gene: 116694534

Centisome position: 22.42

GC content: 70.58

Gene sequence:

>1200_bases
ATGTCTTCCGTCCATTTCCCCGCCGGCCTGCCCGCACGGTTTGCTGCCCTGTCCCTGTGCGCGCTGGCGCTGGCCGCCTG
CGGCAAGTCTGAAGCGCCCAAGGCCGATGACGATCCCAAGGTCAGCGGCAATACCATCCAGTTCCCGGCCAGCGTGAAGA
CGCTGCCGGGCATCGCCGGCGAAGCCGCCAGGACCGGCGGCGAGCGCATGCTGAGCCTGCCCGGCCGGCTGGTGTGGAAC
GAGGACAAGACCGTGCGCGTGTCGACGCCGTTTGCCGGCCGCGTCACCGAGATCCTGGTGCAGCCCGGCGCCACCGTGAA
AGCGGGCCAGCCGCTGGCCAGCCTGACCTCGCCCGATTTCGGCGTGGCCCAGGCCGATGCGCGCAAGGCCGCCGCCGACA
GCGCGGTCGCGGCCAAGGCGCTGGCACGGCAGCGCGAGCTGTACGGCGCCGGCATCATCGCGCAGAAGGAGCTGGAGCAG
GCGCAGGCCGACGCCGCGCGCGCCGCGGCGGACCTGCAGCGCACCCAGGCGGCGCTGGCCCAGTACGGCGCGGCCGCGGG
CGGCGCGGGCGCCTCAGGCATCGTAAACCAGCGCTTTCCGCTGCGCAGCCCGATCGACGGCCTGGTGGTCGAACGCAACA
TCAACCTCGGCATGGAGCTGCGCCCCGACCAGCCGCCCGCCGCGCCGTTGTTCCTGGTGACCGATCCGACCACGCTGTGG
GCGCAGGTCGATGCGGGCGAGGCCGACCTGAGCCTGTTCAAGCCGGGCGTGGACGTGCAGCTCACCACCGCTGCCTGGCC
CGGCGAGACCTTCTCCGGCACCGTGGTCAAGATCGCCGACTATGTCGATCCCACCGCGCGCAGTATCAAGGTGCGGCTGA
ACGTGCCCAACCCGGAGCGGCGCCTGAAGGCAGAGATGTTCGTCACCGCGAAGCTGCCGGCCGCGTCGTTCAAGGGCATC
GCCGTGCCGTCCAAGGCCGTGTTCCTGGCCGACAACCGCAACTATGTGTTCGTGCGCACCACCGCCAATACCTTCGAGCG
CCGCCTGGTGCGCGTGGGCGTGACGCTGCCCGGCACCACCGAGCTGCTTGAAGGCGTGAAGGACGGCGAGACCGTGATCA
CCGACGGCAACCTCTACCTGCAGGACATCCTGCGCGATGCCACTGCCGTGAACGCGGCGCGTGCGTCCGAGAAGAAGTGA

Upstream 100 bases:

>100_bases
GCTGCAGACCTCCACGACCGGCACTGCGACAAGCTCGCGTCCCTGAACCGCAGTCCCAGCGAATCCAATGCACTCCCTCC
TGTCACGCATGTCCCGCCGC

Downstream 100 bases:

>100_bases
GGACGGCCGCCACATGATTTCGCGCATCGTCAGCTTTGCCCTGCACCAGAAGCTCTTTGTCTGGCTGGGCCTGCTTATCT
TCGTCGGGGGCGGCCTGGCT

Product: cation/multidrug efflux system, mebrane-fusion component

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSSVHFPAGLPARFAALSLCALALAACGKSEAPKADDDPKVSGNTIQFPASVKTLPGIAGEAARTGGERMLSLPGRLVWN
EDKTVRVSTPFAGRVTEILVQPGATVKAGQPLASLTSPDFGVAQADARKAAADSAVAAKALARQRELYGAGIIAQKELEQ
AQADAARAAADLQRTQAALAQYGAAAGGAGASGIVNQRFPLRSPIDGLVVERNINLGMELRPDQPPAAPLFLVTDPTTLW
AQVDAGEADLSLFKPGVDVQLTTAAWPGETFSGTVVKIADYVDPTARSIKVRLNVPNPERRLKAEMFVTAKLPAASFKGI
AVPSKAVFLADNRNYVFVRTTANTFERRLVRVGVTLPGTTELLEGVKDGETVITDGNLYLQDILRDATAVNAARASEKK

Sequences:

>Translated_399_residues
MSSVHFPAGLPARFAALSLCALALAACGKSEAPKADDDPKVSGNTIQFPASVKTLPGIAGEAARTGGERMLSLPGRLVWN
EDKTVRVSTPFAGRVTEILVQPGATVKAGQPLASLTSPDFGVAQADARKAAADSAVAAKALARQRELYGAGIIAQKELEQ
AQADAARAAADLQRTQAALAQYGAAAGGAGASGIVNQRFPLRSPIDGLVVERNINLGMELRPDQPPAAPLFLVTDPTTLW
AQVDAGEADLSLFKPGVDVQLTTAAWPGETFSGTVVKIADYVDPTARSIKVRLNVPNPERRLKAEMFVTAKLPAASFKGI
AVPSKAVFLADNRNYVFVRTTANTFERRLVRVGVTLPGTTELLEGVKDGETVITDGNLYLQDILRDATAVNAARASEKK
>Mature_398_residues
SSVHFPAGLPARFAALSLCALALAACGKSEAPKADDDPKVSGNTIQFPASVKTLPGIAGEAARTGGERMLSLPGRLVWNE
DKTVRVSTPFAGRVTEILVQPGATVKAGQPLASLTSPDFGVAQADARKAAADSAVAAKALARQRELYGAGIIAQKELEQA
QADAARAAADLQRTQAALAQYGAAAGGAGASGIVNQRFPLRSPIDGLVVERNINLGMELRPDQPPAAPLFLVTDPTTLWA
QVDAGEADLSLFKPGVDVQLTTAAWPGETFSGTVVKIADYVDPTARSIKVRLNVPNPERRLKAEMFVTAKLPAASFKGIA
VPSKAVFLADNRNYVFVRTTANTFERRLVRVGVTLPGTTELLEGVKDGETVITDGNLYLQDILRDATAVNAARASEKK

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=309, Percent_Identity=23.6245954692557, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786787, Length=312, Percent_Identity=25.9615384615385, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41875; Mature: 41744

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVHFPAGLPARFAALSLCALALAACGKSEAPKADDDPKVSGNTIQFPASVKTLPGIAG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
EAARTGGERMLSLPGRLVWNEDKTVRVSTPFAGRVTEILVQPGATVKAGQPLASLTSPDF
HHHHCCCHHEECCCCEEEECCCCEEEEECCCCCCHHHEEECCCCCEECCCCHHHCCCCCC
GVAQADARKAAADSAVAAKALARQRELYGAGIIAQKELEQAQADAARAAADLQRTQAALA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QYGAAAGGAGASGIVNQRFPLRSPIDGLVVERNINLGMELRPDQPPAAPLFLVTDPTTLW
HHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCEEE
AQVDAGEADLSLFKPGVDVQLTTAAWPGETFSGTVVKIADYVDPTARSIKVRLNVPNPER
EEECCCCCCHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCHHH
RLKAEMFVTAKLPAASFKGIAVPSKAVFLADNRNYVFVRTTANTFERRLVRVGVTLPGTT
HHEEEEEEEEECCCCCCCCEECCCCEEEEECCCCEEEEEECHHHHHHHHEEEECCCCCHH
ELLEGVKDGETVITDGNLYLQDILRDATAVNAARASEKK
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SSVHFPAGLPARFAALSLCALALAACGKSEAPKADDDPKVSGNTIQFPASVKTLPGIAG
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
EAARTGGERMLSLPGRLVWNEDKTVRVSTPFAGRVTEILVQPGATVKAGQPLASLTSPDF
HHHHCCCHHEECCCCEEEECCCCEEEEECCCCCCHHHEEECCCCCEECCCCHHHCCCCCC
GVAQADARKAAADSAVAAKALARQRELYGAGIIAQKELEQAQADAARAAADLQRTQAALA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
QYGAAAGGAGASGIVNQRFPLRSPIDGLVVERNINLGMELRPDQPPAAPLFLVTDPTTLW
HHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEEECCCEEE
AQVDAGEADLSLFKPGVDVQLTTAAWPGETFSGTVVKIADYVDPTARSIKVRLNVPNPER
EEECCCCCCHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEECCCHHH
RLKAEMFVTAKLPAASFKGIAVPSKAVFLADNRNYVFVRTTANTFERRLVRVGVTLPGTT
HHEEEEEEEEECCCCCCCCEECCCCEEEEECCCCEEEEEECHHHHHHHHEEEECCCCCHH
ELLEGVKDGETVITDGNLYLQDILRDATAVNAARASEKK
HHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]