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The map label for this gene is acnB [H]

Identifier: 116694519

GI number: 116694519

Start: 637648

End: 640239

Strand: Direct

Name: acnB [H]

Synonym: H16_B0568

Alternate gene names: 116694519

Gene position: 637648-640239 (Clockwise)

Preceding gene: 116694510

Following gene: 116694521

Centisome position: 21.89

GC content: 65.51

Gene sequence:

>2592_bases
ATGCTTGAAAACTATCGCGCACATGTGGCCGAGCGCGCCGCGCTTGGCATCCCCCCTCTGCCCCTGACCGCCAAGCAGAC
GGCCGAGCTGGTCGAACTGCTGAAGAACCCGCCCGCCGGTGAAGAGCAGACCCTGGTGGAGCTGATCACCTACCGCGTGC
CGGCCGGCGTGGATGACGCCGCCAAGGTCAAGGCCTCGTACCTGGCCGCCGTGGCGCTGGGCAAGGAAAAGTGCGCGCTG
ATCTCGCGCGCCAAGGCGACCGAGCTGCTGGGCACCATGCTGGGCGGCTACAACATCTCGCCGCTGATCGAGCTGCTGGA
CGATGCCGAAGTGGGCACCGTCGCCGCCGAGGCGCTGAAGAAGACCCTGCTGATGTTCGACGCCTTCCACGATGTGAAGG
AAAAGGCCGACCAGGGCAATGCCAACGCCAAGGCCGTGCTGCAAAGCTGGGCCGACGCCGAGTGGTTCACCAGCCGTCCG
GAAGTGCCGCAAAGCCTGACCATCACCGTGTTCAAGGTGCCGGGCGAAACCAACACCGACGACCTGTCGCCGGCCCCGGA
CGCCACCACCCGCCCGGACATCCCGATGCACGCGCTGGCCATGCTGAAGAACAAGCGCGAAGGCGCTGCGTTCCAGCCGG
AAGAAGACGGCAAGCGCGGCCCGGTCAAGTTCATTGAATCGCTGAAGGAAAAGGGCCACCTGGTCGCCTACGTGGGAGAC
GTGGTCGGTACCGGCTCCAGCCGCAAGTCCGCCACCAACTCGGTGCTGTGGTTCACCGGCGAAGACATCCCGTTCATCCC
GAACAAGCGCTTCGGCGGCGTGTGCCTGGGCAACAAGATCGCCCCGATCTTCTACAACACCATGGAAGACGCCGGCGCGC
TGCCGATCGAGCTGGACGTGTCGAAGATGGAAATGGGCGACGTGGTCGAACTGCGCCCGTATGAAGGCAAGGCCCTGAAG
GACGGCGCAGTGATCGCCGAGTTCAAGGTCAAGTCCGACGTGCTGTTCGATGAAGTCCGCGCCGGCGGCCGTATTCCCCT
GATCGTCGGCCGTGGCCTGACCGCCAAGGCGCGCGAAGCGCTGGGCCTGGCCCCGTCGACGCTGTTCCGCCTGCCGCAGA
ACCCGGCCGATACCGGCCGCGGCTTCACGCTGGCGCAGAAGATGGTCGGCCGCGCCTGCGGCCTGCCTGAAGGCAAGGGC
ATCCGCCCGGGCACCTACTGCGAACCGAAGATGACCTCGGTGGGCTCGCAGGACACCACCGGCCCGATGACCCGCGACGA
ACTGAAGGACCTGGCCTGCCTGGGCTTCTCGGCCGACCTGGTGATGCAGTCGTTCTGCCACACCGCCGCCTATCCGAAGC
CGGTCGACGTCAAGACCCACCACACGCTGCCCGAGTTCATCAGCACCCGCGGGGGCATCTCGCTGCGCCCGGGCGACGGC
GTGATCCACTCGTGGCTGAACCGCATGCTGCTGCCCGACACCGTCGGCACCGGCGGCGATTCGCACACCCGCTTCCCGAT
CGGCATCAGCTTCCCCGCAGGTTCGGGCCTGGTGGCCTTTGCCGCCGCCACCGGCGTGATGCCGCTGGACATGCCGGAAT
CGGTGCTGGTCCGCTTCAAGGGCAAGATGCAGCCCGGCGTGACGCTGCGCGACCTGGTCAACGCGATTCCGCTGTACGCG
ATCAAGCAAGGCCTGCTGACCGTGGCCAAGCAAGGCAAGAAGAACATCTTCTCGGGCCGCGTCCTGGAAATCGAAGGCCT
GCCCGACCTGAAGGTCGAGCAAGCGTTCGAGCTGTCGGATGCATCGGCTGAGCGCTCGGCCGCCGGTTGCACGGTGCGCC
TGAACAAGGACCCGATCATCGAATACATCAACAGCAACATCACGCTGCTGAAGTGGATGATCGCCCAGGGCTACCAGGAC
CCGCGCAGCCTGCAGCGCCGCATCAAGGCCATGGAAGCGTGGCTGGCCGATCCCAAGCTGCTGGAGCCGGACGCCGACGC
CGAATACGCCGCCGTGATCGAGATCGACCTGGCCGACGTGCATGAGCCGATCGTGGCCTGCCCGAACGACCCGGACGACG
TCAAGACGCTGTCCGAGGTGGCCGGCGCCAAGATCGACGAAGTGTTTATCGGTTCGTGCATGACCAACATCGGCCACTTC
CGCGCAGCCTCCAAGCTGCTGGAAGGCAAGCGCGACATCCCGGTCAAGCTGTGGGTGGCTCCGCCGACCAAGATGGACCA
GAAGCAGCTGACCGAGGAAGGCCACTATGGCGTGTTCGGCACCGCCGGTGCCCGCACCGAAATGCCGGGCTGCTCGCTGT
GCATGGGCAACCAGGCACAGGTGCGCGAAGGCGCCACGGTGATGTCGACCAGCACGCGTAACTTCCCGAACCGCCTGGGC
AAGAACACCAACGTGTACCTGGGTTCCGCCGAACTGGCGGCGATCTGCTCGCGCCTGGGCCGTATCCCGACCAAGGAAGA
GTACATGGCCGACATGGGCGTGCTCAACGCCAATGGCGACAAGATCTACAAGTACATGAACTTCGACCAGATCGAGGACT
TCAAGGAAGTGGCCGACGGCGTGACGGTGTAA

Upstream 100 bases:

>100_bases
CACTCTTATATAAGATATAAGACATTTGACCGGGGAGCGGCTTCAGACTACAATCGCGACGTAACGAGATTTTCTAAACC
TAATTACTCAGCAGGTTCCC

Downstream 100 bases:

>100_bases
GCCGCGCAGTCAGGCTGGCATGAAGAAGGGGCCCCGCTTGCGGGGCCCTTTTGTATTTGGATTTCGCCGGCTTGACGGGT
GGCATACGCCACCACCACCA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MLENYRAHVAERAALGIPPLPLTAKQTAELVELLKNPPAGEEQTLVELITYRVPAGVDDAAKVKASYLAAVALGKEKCAL
ISRAKATELLGTMLGGYNISPLIELLDDAEVGTVAAEALKKTLLMFDAFHDVKEKADQGNANAKAVLQSWADAEWFTSRP
EVPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKEKGHLVAYVGD
VVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGNKIAPIFYNTMEDAGALPIELDVSKMEMGDVVELRPYEGKALK
DGAVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLPQNPADTGRGFTLAQKMVGRACGLPEGKG
IRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGGISLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYA
IKQGLLTVAKQGKKNIFSGRVLEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKDPIIEYINSNITLLKWMIAQGYQD
PRSLQRRIKAMEAWLADPKLLEPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGSCMTNIGHF
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNTNVYLGSAELAAICSRLGRIPTKEEYMADMGVLNANGDKIYKYMNFDQIEDFKEVADGVTV

Sequences:

>Translated_863_residues
MLENYRAHVAERAALGIPPLPLTAKQTAELVELLKNPPAGEEQTLVELITYRVPAGVDDAAKVKASYLAAVALGKEKCAL
ISRAKATELLGTMLGGYNISPLIELLDDAEVGTVAAEALKKTLLMFDAFHDVKEKADQGNANAKAVLQSWADAEWFTSRP
EVPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKEKGHLVAYVGD
VVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGNKIAPIFYNTMEDAGALPIELDVSKMEMGDVVELRPYEGKALK
DGAVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLPQNPADTGRGFTLAQKMVGRACGLPEGKG
IRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGGISLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYA
IKQGLLTVAKQGKKNIFSGRVLEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKDPIIEYINSNITLLKWMIAQGYQD
PRSLQRRIKAMEAWLADPKLLEPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGSCMTNIGHF
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNTNVYLGSAELAAICSRLGRIPTKEEYMADMGVLNANGDKIYKYMNFDQIEDFKEVADGVTV
>Mature_863_residues
MLENYRAHVAERAALGIPPLPLTAKQTAELVELLKNPPAGEEQTLVELITYRVPAGVDDAAKVKASYLAAVALGKEKCAL
ISRAKATELLGTMLGGYNISPLIELLDDAEVGTVAAEALKKTLLMFDAFHDVKEKADQGNANAKAVLQSWADAEWFTSRP
EVPQSLTITVFKVPGETNTDDLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKEKGHLVAYVGD
VVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGNKIAPIFYNTMEDAGALPIELDVSKMEMGDVVELRPYEGKALK
DGAVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREALGLAPSTLFRLPQNPADTGRGFTLAQKMVGRACGLPEGKG
IRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGGISLRPGDG
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYA
IKQGLLTVAKQGKKNIFSGRVLEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKDPIIEYINSNITLLKWMIAQGYQD
PRSLQRRIKAMEAWLADPKLLEPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGSCMTNIGHF
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
KNTNVYLGSAELAAICSRLGRIPTKEEYMADMGVLNANGDKIYKYMNFDQIEDFKEVADGVTV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=864, Percent_Identity=73.2638888888889, Blast_Score=1270, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=378, Percent_Identity=25.3968253968254, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321429, Length=357, Percent_Identity=26.0504201680672, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93127; Mature: 93127

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENYRAHVAERAALGIPPLPLTAKQTAELVELLKNPPAGEEQTLVELITYRVPAGVDDA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHH
AKVKASYLAAVALGKEKCALISRAKATELLGTMLGGYNISPLIELLDDAEVGTVAAEALK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDAFHDVKEKADQGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKEKGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECH
VVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGNKIAPIFYNTMEDAGALPIELDV
HHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHEEECHHHCCCCEEEEEH
SKMEMGDVVELRPYEGKALKDGAVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREA
HHHCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
LGLAPSTLFRLPQNPADTGRGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMTSVGSQDTT
HCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGGISLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCH
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCC
GKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKKNIFSGRVLEIEGLPDLKVEQAFELSD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHCCCC
ASAERSAAGCTVRLNKDPIIEYINSNITLLKWMIAQGYQDPRSLQRRIKAMEAWLADPKL
CCCHHCCCCCEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LEPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHH
VREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGRIPTKEEYMADMGVLNANGD
HHCCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHCEEECCCC
KIYKYMNFDQIEDFKEVADGVTV
CEEEECCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLENYRAHVAERAALGIPPLPLTAKQTAELVELLKNPPAGEEQTLVELITYRVPAGVDDA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHH
AKVKASYLAAVALGKEKCALISRAKATELLGTMLGGYNISPLIELLDDAEVGTVAAEALK
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDAFHDVKEKADQGNANAKAVLQSWADAEWFTSRPEVPQSLTITVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPMHALAMLKNKREGAAFQPEEDGKRGPVKFIESLKEKGHLVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECH
VVGTGSSRKSATNSVLWFTGEDIPFIPNKRFGGVCLGNKIAPIFYNTMEDAGALPIELDV
HHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHEEECHHHCCCCEEEEEH
SKMEMGDVVELRPYEGKALKDGAVIAEFKVKSDVLFDEVRAGGRIPLIVGRGLTAKAREA
HHHCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEECCCCCHHHHHH
LGLAPSTLFRLPQNPADTGRGFTLAQKMVGRACGLPEGKGIRPGTYCEPKMTSVGSQDTT
HCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCC
GPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHHTLPEFISTRGGISLRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCH
VIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHHCC
GKMQPGVTLRDLVNAIPLYAIKQGLLTVAKQGKKNIFSGRVLEIEGLPDLKVEQAFELSD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHCCCC
ASAERSAAGCTVRLNKDPIIEYINSNITLLKWMIAQGYQDPRSLQRRIKAMEAWLADPKL
CCCHHCCCCCEEEECCCHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
LEPDADAEYAAVIEIDLADVHEPIVACPNDPDDVKTLSEVAGAKIDEVFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RAASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHH
VREGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGRIPTKEEYMADMGVLNANGD
HHCCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHCEEECCCC
KIYKYMNFDQIEDFKEVADGVTV
CEEEECCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]