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The map label for this gene is gcvA [H]

Identifier: 116694481

GI number: 116694481

Start: 597892

End: 598869

Strand: Direct

Name: gcvA [H]

Synonym: H16_B0530

Alternate gene names: 116694481

Gene position: 597892-598869 (Clockwise)

Preceding gene: 116694478

Following gene: 116694484

Centisome position: 20.53

GC content: 66.67

Gene sequence:

>978_bases
ATGGATGACAATGCCAGCACGCGAACGGCCCCTCAAACCGAAGTCGGCTTCCCGTCACGCCCTACCGCCATGAAAAGTAC
AAACGGCCGCCACCTGCCTGCGCTGGCGAACCTGATCGCATTCGAGACCACGGCCCGCACGGGCAGCGTCACCGGGGCGG
CCGAGGAACTCTGCCTGACGCAGAGCGCGGTCAGCAAGCAGATCACCGAGCTGGAATCGTTCCTCGGCGCGCGCATGCTG
AACCGGCGCAAAGGCGCGGTGGCGGCGACACCCGCGGGCGAGGATTACCTCAGGAACGTGCGCAAGGCGCTGGCGCTGCT
GGAGGAAGCGACCATGGAGGTGCAGTCCGGCCGCGCCGCGGGCGGGCGGCTGAACCTGTCGGTGCCGGTTTCGCTGGGCA
ATATCTGGCTGTTGCCGCGCATTCTCAGGTTCGTCAAGGCGCAGCCGCAGATCCAGCTAAACGTCACGACCAAGGTGGGG
CCGGTCGACCTGGAAAGCACCGGGCTCGATGCGGCGATCATGTATTGCGCGGGGCCGCCGCCACGCCATTTCAGCCTGAA
GGTCATGCCGCTGGTGCTCTATCCGATGTGCGCGCCTGAGTTGCTGCCTGGCGGCGGAACGCTGGCCAAGGCCGTGAAGC
GCTTGCCGCTGCTGCACCAGATCGCCGCGCTGGAGGCCTGGCCCGCCTATCTCCGGCAGGCCGGGATCAAGCTGCCGCAT
CCCGACGACGGCTCACGCTATGGTCTGCTGACCATGGGTCTGCAGGCCGCCGTCAGCGGCATGGGCGTGGCCCTGCTGCC
CGATTATGTGGCGGGTGAAGACATCAAGGCCGGCCGGCTGCTGCGCCTGTCCGACGAGCCCTATGTCTCACCCAAGGCCT
ACTACTTCATCTGTCCGGAGGACAAGCGCGACAACCCGGCGATGCAGACCTTCATCGCGTGGCTGCTTGAGCAAAGCGCC
GATGCAAGCGGGCGCTAG

Upstream 100 bases:

>100_bases
TCGGAGTCATCGGCAATCAGGCGGCATGACGCCAGTATTGGTGCAGCCGTGCCGGCAGGCAATGGAAGTTTTGTCCATGC
TTCTTTCCTTCAGGGAATGA

Downstream 100 bases:

>100_bases
GTGAAGCGCGTCCTCAGCCGGCCTGGTCCGCCTTCACCAGGGTGGAAGTGACGAAGCGCCCGGGACGGGTGCTGGCCGGC
AGGCCGTCGCGCAGGGTGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MDDNASTRTAPQTEVGFPSRPTAMKSTNGRHLPALANLIAFETTARTGSVTGAAEELCLTQSAVSKQITELESFLGARML
NRRKGAVAATPAGEDYLRNVRKALALLEEATMEVQSGRAAGGRLNLSVPVSLGNIWLLPRILRFVKAQPQIQLNVTTKVG
PVDLESTGLDAAIMYCAGPPPRHFSLKVMPLVLYPMCAPELLPGGGTLAKAVKRLPLLHQIAALEAWPAYLRQAGIKLPH
PDDGSRYGLLTMGLQAAVSGMGVALLPDYVAGEDIKAGRLLRLSDEPYVSPKAYYFICPEDKRDNPAMQTFIAWLLEQSA
DASGR

Sequences:

>Translated_325_residues
MDDNASTRTAPQTEVGFPSRPTAMKSTNGRHLPALANLIAFETTARTGSVTGAAEELCLTQSAVSKQITELESFLGARML
NRRKGAVAATPAGEDYLRNVRKALALLEEATMEVQSGRAAGGRLNLSVPVSLGNIWLLPRILRFVKAQPQIQLNVTTKVG
PVDLESTGLDAAIMYCAGPPPRHFSLKVMPLVLYPMCAPELLPGGGTLAKAVKRLPLLHQIAALEAWPAYLRQAGIKLPH
PDDGSRYGLLTMGLQAAVSGMGVALLPDYVAGEDIKAGRLLRLSDEPYVSPKAYYFICPEDKRDNPAMQTFIAWLLEQSA
DASGR
>Mature_325_residues
MDDNASTRTAPQTEVGFPSRPTAMKSTNGRHLPALANLIAFETTARTGSVTGAAEELCLTQSAVSKQITELESFLGARML
NRRKGAVAATPAGEDYLRNVRKALALLEEATMEVQSGRAAGGRLNLSVPVSLGNIWLLPRILRFVKAQPQIQLNVTTKVG
PVDLESTGLDAAIMYCAGPPPRHFSLKVMPLVLYPMCAPELLPGGGTLAKAVKRLPLLHQIAALEAWPAYLRQAGIKLPH
PDDGSRYGLLTMGLQAAVSGMGVALLPDYVAGEDIKAGRLLRLSDEPYVSPKAYYFICPEDKRDNPAMQTFIAWLLEQSA
DASGR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=34.3537414965986, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=27.3356401384083, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=26.5993265993266, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI145693193, Length=295, Percent_Identity=24.406779661017, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=26.4026402640264, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=291, Percent_Identity=25.085910652921, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787589, Length=306, Percent_Identity=24.8366013071895, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34930; Mature: 34930

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDNASTRTAPQTEVGFPSRPTAMKSTNGRHLPALANLIAFETTARTGSVTGAAEELCLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QSAVSKQITELESFLGARMLNRRKGAVAATPAGEDYLRNVRKALALLEEATMEVQSGRAA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGRLNLSVPVSLGNIWLLPRILRFVKAQPQIQLNVTTKVGPVDLESTGLDAAIMYCAGPP
CCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCC
PRHFSLKVMPLVLYPMCAPELLPGGGTLAKAVKRLPLLHQIAALEAWPAYLRQAGIKLPH
CCCEEEEEHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDDGSRYGLLTMGLQAAVSGMGVALLPDYVAGEDIKAGRLLRLSDEPYVSPKAYYFICPE
CCCCCEEEHHHHHHHHHHHCCCCEECCHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCC
DKRDNPAMQTFIAWLLEQSADASGR
CCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDDNASTRTAPQTEVGFPSRPTAMKSTNGRHLPALANLIAFETTARTGSVTGAAEELCLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QSAVSKQITELESFLGARMLNRRKGAVAATPAGEDYLRNVRKALALLEEATMEVQSGRAA
HHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGRLNLSVPVSLGNIWLLPRILRFVKAQPQIQLNVTTKVGPVDLESTGLDAAIMYCAGPP
CCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCEEEEEECCCC
PRHFSLKVMPLVLYPMCAPELLPGGGTLAKAVKRLPLLHQIAALEAWPAYLRQAGIKLPH
CCCEEEEEHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PDDGSRYGLLTMGLQAAVSGMGVALLPDYVAGEDIKAGRLLRLSDEPYVSPKAYYFICPE
CCCCCEEEHHHHHHHHHHHCCCCEECCHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCC
DKRDNPAMQTFIAWLLEQSADASGR
CCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]