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The map label for this gene is gatA2 [H]

Identifier: 116694449

GI number: 116694449

Start: 562763

End: 564106

Strand: Direct

Name: gatA2 [H]

Synonym: H16_B0498

Alternate gene names: 116694449

Gene position: 562763-564106 (Clockwise)

Preceding gene: 116694448

Following gene: 116694450

Centisome position: 19.32

GC content: 72.25

Gene sequence:

>1344_bases
ATGGAAGCGACTATCGCAACCGCGCAGCGCCAGCTTGCCGCGGGCAGCGCCAACGCGCTGTCCCTGCTGGCGTCATGCGT
CGAACGTATCTGCAATCCAGGCGGCGAGGGCGCGCGCGTATTCCCGCATGGCGTGAGCATGGCGGCGCGCAAGCAGGCCC
AGGCCAGCGACATGCTGCGCGAGCAGGGCGTGGCCGGCCCGCTGGCGGGCGTGCCGGTGTCCGTGAAGGACCTGTTCGAC
GTGCAGGGCGACGTGACTCGCGCCGGCGCACGTGTGCTGCCGGAGCGTGCGGCCAGTGCCGACGCGCCGGTCATTGCGCG
CTTGCGTGCGGCGGGGGCGGTGTTTGTCGGGCGTACCAATATGACCGAGTTCGCCTACTCGGGCGTCGGCATCAATCCGC
ACTACGGCACGCCACGCAATCCCTACGACCGGCAGGCGGGCCGCATTCCCGGTGGCTCGTCGTCGGGGGCTGCGGTGTCG
GTGACCGACGGCATGGCCCTGGCCGCGATCGGCTCCGACACCGGCGGTTCGTGCCGGATCCCGGCCGCGTTGTGCGGCAT
CGCCGGCTTCAAGCCCACGGCGCGGCGCGTGCCGCTGCAGGGCACGGTGCCGTTGTCGCCCAGCTATGACTCGGTGGGCT
GCCTGGCCAACACTGTGGCGTGCTGCGCCGCAGTTGATGCCGTGATGGCGGGCGAGGTGCCGCCCGCGGGGCCGGCGCCG
CTGGCAGGGCTGCGCCTGGCGGTGCCCACCGGCCTGGTGCTGGAAGGCATGTCGGACGAGGTGGCCGAGGTCTTCAGCCG
CACGCTGCGCCGGCTGTCCGCGGCGGGCGCGCTGCTGCAGGACGTGAGCTTCGACAGCTGGGACCAGCTGGCCGGCATCG
GCGCGAACGGCGGCCTGGTCGCAGCGGAAGCCAGTGCCTGGCACGGCAGCCTGCTGGCTGAGCAGGCCGATCGATATGAC
CCGCGCGTGCTGTCGCGCATCCGGCTGGCTGACGGGCAGCGTGCCGCCGACTACCTGCGCATGCTGCAGCGCCGGGGCGA
GCTGTGCCGGCAGGCCGATGCCGAGCTGGCGGGATTCGATGCCGTGGTCCTGCCCACCGTGCCGGTCGTGGCGCCGCGCA
TCGCGGACCTGGACGACGACGAAGCCTTCTTCAGCACCAACCGGCTGCTGCTGCGCAACCCGGCCATCGCCAACATGCTC
GACCTGTGCGCGCTGTCGGTCCCGTGCCATCGCCCGGGCGACGCACCGGTGGGGCTGATGCTGTTCGGGCGCCACCTGTC
CGACCGCAGGCTGCTGGCCATCGGCATGGGCATCGAGGGGGCATTACGGCACGAGCGGGGTTGA

Upstream 100 bases:

>100_bases
ACACCTACGCCTGCCGCCCGCCCGGCGCCTTCCATGGCCCGTTCAAGTCAGACGGCGGCTGCATGCTGCTGGAGATCCAC
TACTACGACCCGGCCTGATC

Downstream 100 bases:

>100_bases
GCCCCCGCAGCAGTACAGAAGTACCCATAACTACAGTGGAGACAACAATGCCTGATATCCGTACCGGCGCCGCCGTACTG
GCCTGCGCCACCCTGTTCGC

Product: glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MEATIATAQRQLAAGSANALSLLASCVERICNPGGEGARVFPHGVSMAARKQAQASDMLREQGVAGPLAGVPVSVKDLFD
VQGDVTRAGARVLPERAASADAPVIARLRAAGAVFVGRTNMTEFAYSGVGINPHYGTPRNPYDRQAGRIPGGSSSGAAVS
VTDGMALAAIGSDTGGSCRIPAALCGIAGFKPTARRVPLQGTVPLSPSYDSVGCLANTVACCAAVDAVMAGEVPPAGPAP
LAGLRLAVPTGLVLEGMSDEVAEVFSRTLRRLSAAGALLQDVSFDSWDQLAGIGANGGLVAAEASAWHGSLLAEQADRYD
PRVLSRIRLADGQRAADYLRMLQRRGELCRQADAELAGFDAVVLPTVPVVAPRIADLDDDEAFFSTNRLLLRNPAIANML
DLCALSVPCHRPGDAPVGLMLFGRHLSDRRLLAIGMGIEGALRHERG

Sequences:

>Translated_447_residues
MEATIATAQRQLAAGSANALSLLASCVERICNPGGEGARVFPHGVSMAARKQAQASDMLREQGVAGPLAGVPVSVKDLFD
VQGDVTRAGARVLPERAASADAPVIARLRAAGAVFVGRTNMTEFAYSGVGINPHYGTPRNPYDRQAGRIPGGSSSGAAVS
VTDGMALAAIGSDTGGSCRIPAALCGIAGFKPTARRVPLQGTVPLSPSYDSVGCLANTVACCAAVDAVMAGEVPPAGPAP
LAGLRLAVPTGLVLEGMSDEVAEVFSRTLRRLSAAGALLQDVSFDSWDQLAGIGANGGLVAAEASAWHGSLLAEQADRYD
PRVLSRIRLADGQRAADYLRMLQRRGELCRQADAELAGFDAVVLPTVPVVAPRIADLDDDEAFFSTNRLLLRNPAIANML
DLCALSVPCHRPGDAPVGLMLFGRHLSDRRLLAIGMGIEGALRHERG
>Mature_447_residues
MEATIATAQRQLAAGSANALSLLASCVERICNPGGEGARVFPHGVSMAARKQAQASDMLREQGVAGPLAGVPVSVKDLFD
VQGDVTRAGARVLPERAASADAPVIARLRAAGAVFVGRTNMTEFAYSGVGINPHYGTPRNPYDRQAGRIPGGSSSGAAVS
VTDGMALAAIGSDTGGSCRIPAALCGIAGFKPTARRVPLQGTVPLSPSYDSVGCLANTVACCAAVDAVMAGEVPPAGPAP
LAGLRLAVPTGLVLEGMSDEVAEVFSRTLRRLSAAGALLQDVSFDSWDQLAGIGANGGLVAAEASAWHGSLLAEQADRYD
PRVLSRIRLADGQRAADYLRMLQRRGELCRQADAELAGFDAVVLPTVPVVAPRIADLDDDEAFFSTNRLLLRNPAIANML
DLCALSVPCHRPGDAPVGLMLFGRHLSDRRLLAIGMGIEGALRHERG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=454, Percent_Identity=25.3303964757709, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI195972892, Length=140, Percent_Identity=40, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI166795287, Length=136, Percent_Identity=32.3529411764706, Blast_Score=66, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=464, Percent_Identity=26.7241379310345, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=191, Percent_Identity=34.0314136125654, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17537465, Length=465, Percent_Identity=24.7311827956989, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556276, Length=192, Percent_Identity=35.9375, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=192, Percent_Identity=35.9375, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=135, Percent_Identity=40, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=173, Percent_Identity=34.6820809248555, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=391, Percent_Identity=30.9462915601023, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323950, Length=436, Percent_Identity=24.7706422018349, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648113, Length=254, Percent_Identity=35.0393700787402, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45550774, Length=174, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=174, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648439, Length=174, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648435, Length=174, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648437, Length=174, Percent_Identity=33.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24652985, Length=476, Percent_Identity=23.109243697479, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922090, Length=476, Percent_Identity=23.109243697479, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652981, Length=476, Percent_Identity=23.109243697479, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24652983, Length=476, Percent_Identity=23.109243697479, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356731, Length=149, Percent_Identity=33.5570469798658, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644968, Length=140, Percent_Identity=35, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI161078093, Length=212, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 46242; Mature: 46242

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATIATAQRQLAAGSANALSLLASCVERICNPGGEGARVFPHGVSMAARKQAQASDMLR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHH
EQGVAGPLAGVPVSVKDLFDVQGDVTRAGARVLPERAASADAPVIARLRAAGAVFVGRTN
HCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCC
MTEFAYSGVGINPHYGTPRNPYDRQAGRIPGGSSSGAAVSVTDGMALAAIGSDTGGSCRI
CHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEC
PAALCGIAGFKPTARRVPLQGTVPLSPSYDSVGCLANTVACCAAVDAVMAGEVPPAGPAP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LAGLRLAVPTGLVLEGMSDEVAEVFSRTLRRLSAAGALLQDVSFDSWDQLAGIGANGGLV
CCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEE
AAEASAWHGSLLAEQADRYDPRVLSRIRLADGQRAADYLRMLQRRGELCRQADAELAGFD
EEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
AVVLPTVPVVAPRIADLDDDEAFFSTNRLLLRNPAIANMLDLCALSVPCHRPGDAPVGLM
EEHHCCCCHHCCHHCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEHH
LFGRHLSDRRLLAIGMGIEGALRHERG
HHCCCCCCCEEEEEECCCCHHHHCCCC
>Mature Secondary Structure
MEATIATAQRQLAAGSANALSLLASCVERICNPGGEGARVFPHGVSMAARKQAQASDMLR
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHH
EQGVAGPLAGVPVSVKDLFDVQGDVTRAGARVLPERAASADAPVIARLRAAGAVFVGRTN
HCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEEECCC
MTEFAYSGVGINPHYGTPRNPYDRQAGRIPGGSSSGAAVSVTDGMALAAIGSDTGGSCRI
CHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCEEC
PAALCGIAGFKPTARRVPLQGTVPLSPSYDSVGCLANTVACCAAVDAVMAGEVPPAGPAP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LAGLRLAVPTGLVLEGMSDEVAEVFSRTLRRLSAAGALLQDVSFDSWDQLAGIGANGGLV
CCCEEEECCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCEE
AAEASAWHGSLLAEQADRYDPRVLSRIRLADGQRAADYLRMLQRRGELCRQADAELAGFD
EEECCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCC
AVVLPTVPVVAPRIADLDDDEAFFSTNRLLLRNPAIANMLDLCALSVPCHRPGDAPVGLM
EEHHCCCCHHCCHHCCCCCCHHHHHCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCEEHH
LFGRHLSDRRLLAIGMGIEGALRHERG
HHCCCCCCCEEEEEECCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA