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The map label for this gene is hipO [H]

Identifier: 116694442

GI number: 116694442

Start: 556275

End: 557447

Strand: Direct

Name: hipO [H]

Synonym: H16_B0491

Alternate gene names: 116694442

Gene position: 556275-557447 (Clockwise)

Preceding gene: 116694441

Following gene: 116694444

Centisome position: 19.1

GC content: 67.43

Gene sequence:

>1173_bases
ATGTACCGCGACCCCGATATTGCCGACGACATCCGCCAGGACATGCTGGCCTGGCGCCGCGACATCCACGCTGTTCCGGA
AACCGCATTCGAAGAACACCGCACCGCTGGCATCGTGGCGCAGGCGCTGGGTTTGATGGACATCGAAGTGCAGCGGGGAC
TGGCGACGACGGGCGTGGTCGGCACGCTGCGCAACGGCGACGGCCCCAGCATCGCGCTGCGCGCGGACCTGGATGCGCTG
AACATGCAGGAGCTGGGCAAGGCCGCCCACGCTTCCACCTGCGCGGGCAAGATGCACGCCTGTGGCCACGACGGCCATAC
CGCCATGCTGCTGGGTGCGGCCGCGCACCTGTCGCGCAACCGCGATGCCTTCCGCGGCACGGTGCATTTCGTGTTCCAGC
CCGCCGAAGAGAATGAAGGCGGCGGCCGCGTGATGGTGGAAGAGGGCCTGTTCGACCAGTTCCCGGCCGATGCCGTCTAT
GGCATGCACAACTTCCCCCAGTTGCCACGCGGCAAGTTCGCCATCCGCGCCGGCACCATGACCGCGTTCCTGGACAACTT
CGAGATCGTCATCACCGGCAAGGGCGCGCATGGCGCCCAGCCGCACCACGGCATCGATTCCGTGGTCGTGTCCGCGCACC
TGATCACCGCGCTGCAGACCATTGCCAGCCGCCGCACCGATCCCACCGATTCGGTCGTGGTCAGCATCACGCAGATCCAC
GGCGGCGATACCTGGAACGTGCTGCCTGAGTCGGTGGTGCTGCGCGGCACCATCCGCACGCTGAACCCCGCCGTGCGGGA
TCGCACGCAGACGGCGATGCGGCAGATCTGCGAGGGCGTGGGCACGACCCACGAGGCGCGGATCGCGATTGACTACCGCC
CCGGCTACCCCGGCGTGGTCAACACCCCCGCCGAGACCGACGCCGCCATTGCCGCCGCCGCGCAACTGGTGGGCGCCGAC
AACGTGAAGACCGATATCAAGCCGGCAATGGGATCGGAGGACTTCGCCTTCATGCTGGAAAAGCGCCCCGGCGCTTATAT
CGGCATCGGCGCCGGCGAGAGCGCGGAAGACCCGCCCCTGCACAATCCCTACTATGACTTCAACGACAGGATCCTGCCGC
TGGGCGCGGCGTATTGGGTGGCGCTGGTCGCGCAGCAATTGCCGGCCAGATGA

Upstream 100 bases:

>100_bases
ATCGTGGTGGTGTCGCTGACGGCCACGGCCGGCACCTTCCTCGGCGCCACGCTGATTGCCGAGTTCTTCCACTGAATCCC
ACCCGTAGCAGAGATTACCC

Downstream 100 bases:

>100_bases
TCCGCGCCCGTCAGGACCTGCGGGCTGCTTGCGCGAACACGTTCAGGAAGCCCAGCGCGGGCTGCGCGCCGGTGTGCGCT
TCGATGGCGGCAGCCAGCAC

Product: aminoacylase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MYRDPDIADDIRQDMLAWRRDIHAVPETAFEEHRTAGIVAQALGLMDIEVQRGLATTGVVGTLRNGDGPSIALRADLDAL
NMQELGKAAHASTCAGKMHACGHDGHTAMLLGAAAHLSRNRDAFRGTVHFVFQPAEENEGGGRVMVEEGLFDQFPADAVY
GMHNFPQLPRGKFAIRAGTMTAFLDNFEIVITGKGAHGAQPHHGIDSVVVSAHLITALQTIASRRTDPTDSVVVSITQIH
GGDTWNVLPESVVLRGTIRTLNPAVRDRTQTAMRQICEGVGTTHEARIAIDYRPGYPGVVNTPAETDAAIAAAAQLVGAD
NVKTDIKPAMGSEDFAFMLEKRPGAYIGIGAGESAEDPPLHNPYYDFNDRILPLGAAYWVALVAQQLPAR

Sequences:

>Translated_390_residues
MYRDPDIADDIRQDMLAWRRDIHAVPETAFEEHRTAGIVAQALGLMDIEVQRGLATTGVVGTLRNGDGPSIALRADLDAL
NMQELGKAAHASTCAGKMHACGHDGHTAMLLGAAAHLSRNRDAFRGTVHFVFQPAEENEGGGRVMVEEGLFDQFPADAVY
GMHNFPQLPRGKFAIRAGTMTAFLDNFEIVITGKGAHGAQPHHGIDSVVVSAHLITALQTIASRRTDPTDSVVVSITQIH
GGDTWNVLPESVVLRGTIRTLNPAVRDRTQTAMRQICEGVGTTHEARIAIDYRPGYPGVVNTPAETDAAIAAAAQLVGAD
NVKTDIKPAMGSEDFAFMLEKRPGAYIGIGAGESAEDPPLHNPYYDFNDRILPLGAAYWVALVAQQLPAR
>Mature_390_residues
MYRDPDIADDIRQDMLAWRRDIHAVPETAFEEHRTAGIVAQALGLMDIEVQRGLATTGVVGTLRNGDGPSIALRADLDAL
NMQELGKAAHASTCAGKMHACGHDGHTAMLLGAAAHLSRNRDAFRGTVHFVFQPAEENEGGGRVMVEEGLFDQFPADAVY
GMHNFPQLPRGKFAIRAGTMTAFLDNFEIVITGKGAHGAQPHHGIDSVVVSAHLITALQTIASRRTDPTDSVVVSITQIH
GGDTWNVLPESVVLRGTIRTLNPAVRDRTQTAMRQICEGVGTTHEARIAIDYRPGYPGVVNTPAETDAAIAAAAQLVGAD
NVKTDIKPAMGSEDFAFMLEKRPGAYIGIGAGESAEDPPLHNPYYDFNDRILPLGAAYWVALVAQQLPAR

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=420, Percent_Identity=30, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41865; Mature: 41865

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRDPDIADDIRQDMLAWRRDIHAVPETAFEEHRTAGIVAQALGLMDIEVQRGLATTGVV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEHHCCCCCCCCE
GTLRNGDGPSIALRADLDALNMQELGKAAHASTCAGKMHACGHDGHTAMLLGAAAHLSRN
EEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCC
RDAFRGTVHFVFQPAEENEGGGRVMVEEGLFDQFPADAVYGMHNFPQLPRGKFAIRAGTM
CHHHEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCC
TAFLDNFEIVITGKGAHGAQPHHGIDSVVVSAHLITALQTIASRRTDPTDSVVVSITQIH
EEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEC
GGDTWNVLPESVVLRGTIRTLNPAVRDRTQTAMRQICEGVGTTHEARIAIDYRPGYPGVV
CCCCCCCCCCHHEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
NTPAETDAAIAAAAQLVGADNVKTDIKPAMGSEDFAFMLEKRPGAYIGIGAGESAEDPPL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCC
HNPYYDFNDRILPLGAAYWVALVAQQLPAR
CCCCCCCCCCEEECHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYRDPDIADDIRQDMLAWRRDIHAVPETAFEEHRTAGIVAQALGLMDIEVQRGLATTGVV
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEHHCCCCCCCCE
GTLRNGDGPSIALRADLDALNMQELGKAAHASTCAGKMHACGHDGHTAMLLGAAAHLSRN
EEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCC
RDAFRGTVHFVFQPAEENEGGGRVMVEEGLFDQFPADAVYGMHNFPQLPRGKFAIRAGTM
CHHHEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCCCCCEEEEECCC
TAFLDNFEIVITGKGAHGAQPHHGIDSVVVSAHLITALQTIASRRTDPTDSVVVSITQIH
EEEECCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEC
GGDTWNVLPESVVLRGTIRTLNPAVRDRTQTAMRQICEGVGTTHEARIAIDYRPGYPGVV
CCCCCCCCCCHHEEEHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
NTPAETDAAIAAAAQLVGADNVKTDIKPAMGSEDFAFMLEKRPGAYIGIGAGESAEDPPL
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCCCCCC
HNPYYDFNDRILPLGAAYWVALVAQQLPAR
CCCCCCCCCCEEECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]