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The map label for this gene is ldh [H]

Identifier: 116694400

GI number: 116694400

Start: 508285

End: 509382

Strand: Direct

Name: ldh [H]

Synonym: H16_B0449

Alternate gene names: 116694400

Gene position: 508285-509382 (Clockwise)

Preceding gene: 116694399

Following gene: 116694403

Centisome position: 17.45

GC content: 68.49

Gene sequence:

>1098_bases
ATGGAGAAGAAGTTGAACGACCGTACCCACGCGGCGGCCGCTGTCCGCCTTTTCGGCACCGGCGAAGGCCTGTCCCACGA
GCGCGTAACGCTCGCCACCGATCCGGAATCCGGCCTGCAGGCCATCATCGCCATCTACAACACCAGACTGGGCCCCGCAT
TCGGCGGCTGCCGGTACTGGGCCTATCCCGCCGAAGCCGACGCGCTGTCCGATGCGCTGCGCCTGTCACAGGGCATGGCC
TACAAGAACGCCCTCGCCGGGCTGCCGTTCGGTGGCGGCAAGGCGGTGATCCTGCGTTCCCCGGCGCAGACCGACCGCAA
GCAACTCTTCCGCGCCTTCGGCCGCATGATCGAGTCGCTGCAAGGCAGCTATATTACGGCCGAGGATGTCGGCACGACGG
TGGACGACATGCGTGCCGTTCAGGCCGAGACCCGCTACGTCAGCGGCATGCCGCGCGACGGCGGCTTCGGCGGCAATCCC
TCGCCGTGGACGGCATACGGCGTGTTCGTCGGCATCGAGGCGGCGGCGCTGTATCGCCTGGACCGCAAGTCGCTCGCCGG
CTTGTCCGTGGCCGTCCAGGGACTGGGTTCGGTGGGCTGGGAGCTTTGCCGCCGTCTCCATGAAGCGGGCGCCACCCTGA
CCGTGGCCGATATCGACGCCGGCCGCACGACGCAGGCGCAACAGCAGTTTGGCGCCCGCGTCGTATCGCCGGAAGCCATC
ACGGCCGTCGACGCCGATGTATTCGCGCCTTGCGCGCTGGGTGCCGTCATCACCCCGGCCGTGGCCGAAAGCTGCCGTTT
CCGGGCCATCGCCGGCGGGGCAAACAATCAACTCGCTTCGCTCGCCGAAGGTGACATCCTGCACCAGCGCGGGATCTTCT
ATGCGCCCGACTTCCTCATCAATGCCGGCGGCATCGTGAGTTGCGCCCGGGAATACCTCGGCGGTGTCGACGACTCGGAC
CTGCGCGACGAAGTGGCAGGCATCCGCGAACGGGTGCTCAGGCTGGCGCAACGCGTGGAGTCGACCGGCCAGGCGCCGGC
GCGCGCGGCGGTGACATGGGCGCACGAGGTGTTGTCGCGTGGCATCGGGCCGAACTGA

Upstream 100 bases:

>100_bases
GAACGGGATTGGGGCTCCAGGCCCCATCAACGCCGCACCGCACTGCAACCCAGGCGGTGTGTCAAGGAAGGCAGAGCTAC
AGCTTTGCATTCCCCACGTC

Downstream 100 bases:

>100_bases
GCGTCGCGTCGACCAGGAAGCCGATGAACGAATTCCCGAAGCCCCACAGTTCGACCAGCGCCAGCGCTGGTTGATGTGCT
GGCTGGGGAATACGCACGGG

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MEKKLNDRTHAAAAVRLFGTGEGLSHERVTLATDPESGLQAIIAIYNTRLGPAFGGCRYWAYPAEADALSDALRLSQGMA
YKNALAGLPFGGGKAVILRSPAQTDRKQLFRAFGRMIESLQGSYITAEDVGTTVDDMRAVQAETRYVSGMPRDGGFGGNP
SPWTAYGVFVGIEAAALYRLDRKSLAGLSVAVQGLGSVGWELCRRLHEAGATLTVADIDAGRTTQAQQQFGARVVSPEAI
TAVDADVFAPCALGAVITPAVAESCRFRAIAGGANNQLASLAEGDILHQRGIFYAPDFLINAGGIVSCAREYLGGVDDSD
LRDEVAGIRERVLRLAQRVESTGQAPARAAVTWAHEVLSRGIGPN

Sequences:

>Translated_365_residues
MEKKLNDRTHAAAAVRLFGTGEGLSHERVTLATDPESGLQAIIAIYNTRLGPAFGGCRYWAYPAEADALSDALRLSQGMA
YKNALAGLPFGGGKAVILRSPAQTDRKQLFRAFGRMIESLQGSYITAEDVGTTVDDMRAVQAETRYVSGMPRDGGFGGNP
SPWTAYGVFVGIEAAALYRLDRKSLAGLSVAVQGLGSVGWELCRRLHEAGATLTVADIDAGRTTQAQQQFGARVVSPEAI
TAVDADVFAPCALGAVITPAVAESCRFRAIAGGANNQLASLAEGDILHQRGIFYAPDFLINAGGIVSCAREYLGGVDDSD
LRDEVAGIRERVLRLAQRVESTGQAPARAAVTWAHEVLSRGIGPN
>Mature_365_residues
MEKKLNDRTHAAAAVRLFGTGEGLSHERVTLATDPESGLQAIIAIYNTRLGPAFGGCRYWAYPAEADALSDALRLSQGMA
YKNALAGLPFGGGKAVILRSPAQTDRKQLFRAFGRMIESLQGSYITAEDVGTTVDDMRAVQAETRYVSGMPRDGGFGGNP
SPWTAYGVFVGIEAAALYRLDRKSLAGLSVAVQGLGSVGWELCRRLHEAGATLTVADIDAGRTTQAQQQFGARVVSPEAI
TAVDADVFAPCALGAVITPAVAESCRFRAIAGGANNQLASLAEGDILHQRGIFYAPDFLINAGGIVSCAREYLGGVDDSD
LRDEVAGIRERVLRLAQRVESTGQAPARAAVTWAHEVLSRGIGPN

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids. Catalyzes the reversible deamination of these branched-chain L-amino acids to their oxo analogs [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=311, Percent_Identity=27.3311897106109, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4885281, Length=311, Percent_Identity=26.6881028938907, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45549226, Length=320, Percent_Identity=28.125, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45553475, Length=320, Percent_Identity=28.125, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 38568; Mature: 38568

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKLNDRTHAAAAVRLFGTGEGLSHERVTLATDPESGLQAIIAIYNTRLGPAFGGCRYW
CCCCCCCHHHHHHEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
AYPAEADALSDALRLSQGMAYKNALAGLPFGGGKAVILRSPAQTDRKQLFRAFGRMIESL
CCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
QGSYITAEDVGTTVDDMRAVQAETRYVSGMPRDGGFGGNPSPWTAYGVFVGIEAAALYRL
CCCEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DRKSLAGLSVAVQGLGSVGWELCRRLHEAGATLTVADIDAGRTTQAQQQFGARVVSPEAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCEECCCHHH
TAVDADVFAPCALGAVITPAVAESCRFRAIAGGANNQLASLAEGDILHQRGIFYAPDFLI
HHCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHCCCHHHHCCCEECCHHHC
NAGGIVSCAREYLGGVDDSDLRDEVAGIRERVLRLAQRVESTGQAPARAAVTWAHEVLSR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GIGPN
CCCCC
>Mature Secondary Structure
MEKKLNDRTHAAAAVRLFGTGEGLSHERVTLATDPESGLQAIIAIYNTRLGPAFGGCRYW
CCCCCCCHHHHHHEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
AYPAEADALSDALRLSQGMAYKNALAGLPFGGGKAVILRSPAQTDRKQLFRAFGRMIESL
CCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
QGSYITAEDVGTTVDDMRAVQAETRYVSGMPRDGGFGGNPSPWTAYGVFVGIEAAALYRL
CCCEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DRKSLAGLSVAVQGLGSVGWELCRRLHEAGATLTVADIDAGRTTQAQQQFGARVVSPEAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCEECCCHHH
TAVDADVFAPCALGAVITPAVAESCRFRAIAGGANNQLASLAEGDILHQRGIFYAPDFLI
HHCCHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHCCCHHHHCCCEECCHHHC
NAGGIVSCAREYLGGVDDSDLRDEVAGIRERVLRLAQRVESTGQAPARAAVTWAHEVLSR
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
GIGPN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8020469 [H]