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The map label for this gene is flgJ [H]

Identifier: 116694224

GI number: 116694224

Start: 303891

End: 304934

Strand: Direct

Name: flgJ [H]

Synonym: H16_B0269

Alternate gene names: 116694224

Gene position: 303891-304934 (Clockwise)

Preceding gene: 116694223

Following gene: 116694225

Centisome position: 10.43

GC content: 69.92

Gene sequence:

>1044_bases
ATGAACACGGCACGACCCCCGCAGGCGGCGGAGCTGACGCAGCGCTTTGCGCTGGACACGCAGGGCTTCGAGGCGCTCAA
GCACAGCGCGCGCGGCGGCGCCGACGCCAATACGCTGCAAGCCGTCGCCAGGCAGTTCGAGGCGGTGTTCACGCAGATGG
TGCTCAAGAGCATGCGCGATGCCACCCCGCAGGACGGCCTCTTCGACAACGAGCAGAGCAAGCTCTATATGTCGATGATG
GACCAGCAGCTTGCGCAGCAGATGTCGTCGCGCGGGATCGGCCTGGCCGATGTGATGGTGCGGCAGCTGGCGCGCGCCAC
GGGCACGCCGATGCCGGCCGGCATGAGCGCCATGAGCCCGGCCGAGTCAGGCAAGGCGGCCGATGCCGAGATGGCAAGAT
TGCTCGACAGCCGCGGCGCCGGGGCGATCCCGGCCGACGCCGGCGAGCAGGCCGACCTGCCGGCGATCGGCACCATCGTG
CCGGGGCAGAACTGGAATCCCACTGCCGGCCTGCGCCAGTACCAGCCGCAGTGGTACGCGGACCGTGGCCAGGGCGAGGA
CAGGCTGGGACGGTTGCCGGACGACGCGCCGTCCCACGTCAGCGCCTTTGTCGCCCGCATGGCCGGGCCCGCGGAAGCCG
CCGCGCGCGCCAGCGGCGTGCCGGCGCGGCTGATCGTAGGCCAGGCCGCGCTGGAATCCGGCTGGGGCCAGCGCGAGATC
ACGCACGCCGACGGCTCCACCACCTTCAACGTCTTCGGCATCAAGGCCGGGGCCAGCTGGAAGGGGCGTGTGGCCGAGAT
CACCACCACGGAATATGTCGACGGGCAGCCGCAGAAGGTGCGGGCGAAGTTTCGCGCCTATGGCTCGTATGACGAGGCCT
GTGCCGACTATGCCCGCCTGCTGACGAACAACCCGCGCTACGCGGGCGTGGTCAGCGCGGCGTCGGCTGAAGAGGCGGCG
CATGGCTTGCAGCGCGCGGGCTATGCCACCGATCCGGCGTACGGGCACAAGCTGGTGAAGATCATGAAGAAGGTCGCAGC
CTGA

Upstream 100 bases:

>100_bases
GGCTGAACGCGCTGGGCGCAACCCCGGCCGACCTGCAGACCATCCTGGAAGCCATGCGCGCGGCCGGGGCGCTGCGCGCC
GAGCTGGAAGTGATCTGACC

Downstream 100 bases:

>100_bases
GGGCAGGGGGAAGCCAGCGGATCTGGCGAAGATCACTTCCACACCTAAAGTCCCGCCCCCACCCAGCCGATAACTTCCAC
ATCCACCACCGCCGGCCCTC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNTARPPQAAELTQRFALDTQGFEALKHSARGGADANTLQAVARQFEAVFTQMVLKSMRDATPQDGLFDNEQSKLYMSMM
DQQLAQQMSSRGIGLADVMVRQLARATGTPMPAGMSAMSPAESGKAADAEMARLLDSRGAGAIPADAGEQADLPAIGTIV
PGQNWNPTAGLRQYQPQWYADRGQGEDRLGRLPDDAPSHVSAFVARMAGPAEAAARASGVPARLIVGQAALESGWGQREI
THADGSTTFNVFGIKAGASWKGRVAEITTTEYVDGQPQKVRAKFRAYGSYDEACADYARLLTNNPRYAGVVSAASAEEAA
HGLQRAGYATDPAYGHKLVKIMKKVAA

Sequences:

>Translated_347_residues
MNTARPPQAAELTQRFALDTQGFEALKHSARGGADANTLQAVARQFEAVFTQMVLKSMRDATPQDGLFDNEQSKLYMSMM
DQQLAQQMSSRGIGLADVMVRQLARATGTPMPAGMSAMSPAESGKAADAEMARLLDSRGAGAIPADAGEQADLPAIGTIV
PGQNWNPTAGLRQYQPQWYADRGQGEDRLGRLPDDAPSHVSAFVARMAGPAEAAARASGVPARLIVGQAALESGWGQREI
THADGSTTFNVFGIKAGASWKGRVAEITTTEYVDGQPQKVRAKFRAYGSYDEACADYARLLTNNPRYAGVVSAASAEEAA
HGLQRAGYATDPAYGHKLVKIMKKVAA
>Mature_347_residues
MNTARPPQAAELTQRFALDTQGFEALKHSARGGADANTLQAVARQFEAVFTQMVLKSMRDATPQDGLFDNEQSKLYMSMM
DQQLAQQMSSRGIGLADVMVRQLARATGTPMPAGMSAMSPAESGKAADAEMARLLDSRGAGAIPADAGEQADLPAIGTIV
PGQNWNPTAGLRQYQPQWYADRGQGEDRLGRLPDDAPSHVSAFVARMAGPAEAAARASGVPARLIVGQAALESGWGQREI
THADGSTTFNVFGIKAGASWKGRVAEITTTEYVDGQPQKVRAKFRAYGSYDEACADYARLLTNNPRYAGVVSAASAEEAA
HGLQRAGYATDPAYGHKLVKIMKKVAA

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=331, Percent_Identity=44.1087613293051, Blast_Score=250, Evalue=9e-68,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36863; Mature: 36863

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTARPPQAAELTQRFALDTQGFEALKHSARGGADANTLQAVARQFEAVFTQMVLKSMRD
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ATPQDGLFDNEQSKLYMSMMDQQLAQQMSSRGIGLADVMVRQLARATGTPMPAGMSAMSP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHCCC
AESGKAADAEMARLLDSRGAGAIPADAGEQADLPAIGTIVPGQNWNPTAGLRQYQPQWYA
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHCCCCHHC
DRGQGEDRLGRLPDDAPSHVSAFVARMAGPAEAAARASGVPARLIVGQAALESGWGQREI
CCCCCHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEE
THADGSTTFNVFGIKAGASWKGRVAEITTTEYVDGQPQKVRAKFRAYGSYDEACADYARL
EECCCCCEEEEEEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LTNNPRYAGVVSAASAEEAAHGLQRAGYATDPAYGHKLVKIMKKVAA
HHCCCCCCHHEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTARPPQAAELTQRFALDTQGFEALKHSARGGADANTLQAVARQFEAVFTQMVLKSMRD
CCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
ATPQDGLFDNEQSKLYMSMMDQQLAQQMSSRGIGLADVMVRQLARATGTPMPAGMSAMSP
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHCCC
AESGKAADAEMARLLDSRGAGAIPADAGEQADLPAIGTIVPGQNWNPTAGLRQYQPQWYA
CCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHCCCCHHC
DRGQGEDRLGRLPDDAPSHVSAFVARMAGPAEAAARASGVPARLIVGQAALESGWGQREI
CCCCCHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEE
THADGSTTFNVFGIKAGASWKGRVAEITTTEYVDGQPQKVRAKFRAYGSYDEACADYARL
EECCCCCEEEEEEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LTNNPRYAGVVSAASAEEAAHGLQRAGYATDPAYGHKLVKIMKKVAA
HHCCCCCCHHEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]