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The map label for this gene is topB [H]

Identifier: 116691148

GI number: 116691148

Start: 3445427

End: 3448024

Strand: Direct

Name: topB [H]

Synonym: Bcen2424_3130

Alternate gene names: 116691148

Gene position: 3445427-3448024 (Clockwise)

Preceding gene: 116691147

Following gene: 116691150

Centisome position: 98.9

GC content: 64.09

Gene sequence:

>2598_bases
ATGTCCAAAGCACTGATCATTGCGGAAAAGCCTTCTGTCGCGAACGACATCGCACGCGCTTTGGGCGGCTTTACCAAGCA
TGACGAGTATTACGAGAGCGACGATTTCGTCCTTTCGTCCGCTGTCGGCCACCTGCTGGAAATCGCCGCCCCGGAGGAGT
ACGAGGTCAAGCGCGGGAAATGGAGCTTCGCGCATCTGCCCGTCATTCCCCCGCATTTCGACCTGAACCCGATCGCAAAA
AGCGAGTCGCGCCTCAAGGTGCTCACGAAGCTGATGAAGCGCAAGGACGTCGACCGCCTGATCAACGCATGTGACGCGGG
GCGCGAAGGCGAGCTGATTTTCCGCCTGATCGCGCAGCACGCGAAGGCGAAGCAGCCGGTCCAGCGCCTGTGGCTGCAAT
CGATGACGCCGCAGGCGATTCGCGATGGTTTCGCGAACCTGCGCAGCGACACGGACATGCAGCCGCTCGCCGACGCCGCA
CGCTGCCGCTCGGAAGCCGACTGGCTCGTCGGGATCAACGGCACCCGCGCGATGACCGCGTTCAACAGCAAGGGCGGCGG
CTTCTTCCTGACCACCGTCGGCCGGGTTCAGACGCCAACGTTGTCGATCGTCGTCGAACGCGAAGAGAAAATCCGCCGCT
TCATCCCGCGCGACTACTGGGAAGTGAAGGCCGAATTCGCGTGCGCGAGCGGCTTCTACGAAGGGAAGTGGTACGACCCG
AAATTCAAGCGCGACGAATTCGATCCGGAAAAGCGCGACTCCCGCCTGTGGAGCCTGCCGGCCGCCGAGACGATCGTCGC
CGCCTGCCGCGACCAGATCGGCACGGTGTCCGAGGAATCGAAGCCGTCGACGCAACTGTCGCCGCTGCTGTTCGACCTGA
CGAGCCTGCAGCGCGAAGCGAACGGCCGTTTCGGCTTCTCCGCGAAGAACACGCTCGGTCTCGCGCAGGCGCTGTATGAA
AAGCACAAGGTGCTGACCTACCCGCGTACCGACGCGCGCGCGCTGCCGGAAGACTACCTGTCGACCGTGCAGTCCACGCT
CGAGATGCTCAAGGAGAGCAACAACTACCTGCCGCACGCGAAGCAGGTGCTCGACAAGGGTTGGGTGAAGCCGAACAAGC
GGATCTTCGACAACTCGAAGATCAGCGACCACTTTGCAATCATCCCGACGCTCCAGGCGCCGAAGGCGCTGTCCGAGCCC
GAGCAGAAGCTGTACGACCTGGTCGTGAAGCGCTTCCTCGCCGTGTTCTTCCCGGCGGCCGAATTCCGCGTGACGACCCG
GATCACCGAAGTCGCCGGCCATCACTTCAAGACCGAAGGCAAGGTGCTGGTCGAGCCGGGCTGGCTGCAGGTGTACGGCC
GCGACGCCGAAGGCGCGGACGCGAACCTCGTGCCGGTGCAGAAGGACGAGAAGGTGAAGACCGACGAGATCGCCGCCGTC
GCGCTCGTGACCAAGCCGCCCGCACGCTACTCGGAAGCGACGCTGCTGTCCGCGATGGAAGGCGCGGGCAAGCTGGTCGA
GGACGACGAGCTGCGCGAGGCGATGGCCGCGAAGGGCCTCGGTACGCCGGCCACGCGTGCGGCGATCATCGAAGGCCTGC
TCGGCGAAAAGTACCTGCTGCGCGAAGGTCGCGAACTGATCCCGACCGCGAAGGCATTCCAGCTGATGACGCTGCTGCGC
GGCCTCGGCGTGAAGGAACTCACGGCGCCCGAGCTCACCGGCGAATGGGAATACAAGCTGTCGCAGATGGAGCGCGGCAA
TCTCGGCCGCGACGCGTTTATGCAGGAAATCGCCCGCATGACGCAGCAGATCGTCAAGCGCGCGAAGGAATACGATTCCG
ACACGATCCCCGGCGATTACGCGACGCTCGAGACACCGTGCCCGAATTGCGGCGGCCAGGTGAAGGAAAACTACCGCCGC
TTCGCGTGCACGAAATGCGACTTCTCGATCTCGAAGATCCCGGGCAGCCGGCAGTTCGAGATCCCGGAAGTCGAAGAGCT
GCTGCAGAAGAAGGAAATCGGGCCGCTGTCCGGGTTCCGCAGCAAGATGGGCCGCCCGTTCTCGGCGATCCTCAAGCTGT
CCTTCGACGACGAGACGAAGAACTACAAGCTCGAGTTCGATTTCGGCCAGGACCAGGGCGGCGAGGAAGGCGAAGCGCCC
GACTTCTCCGCGCAGGAGCCGGTCGGCGCGTGCCCGAAGTGCAAGGGCCGCGTGTTCGAGCACGGGATGAGCTACGTGTG
CGAACACTCGGTGGCGAACCCGAAGACCTGCGACTTCCGCTCGGGCAAGGTGATCCTGCAGCAGGAAATCACGCGCGAGC
AGATGGGCAAGCTGCTCGCCGACGGCCGCACGGATCTGCTGCCGAACTTCAAGTCGTCGCGTACCGGCCGCAACTTCAAG
GCGTTCCTCGTGAAGCAGCCGGACGGCAAGATCGGTTTCGAGTTCGAGAAGAAGGAACCGAAAGCCGCCGCCGCGAAGAA
GACGGCCAAATCGGCGACGAAGGATGCCGAAACCGTGACGGAAGGCGCCGAAGAGAAACCGGCACCGGCCCGCAAGACCG
CCGCCCGCAAGACGACGGCGCGCAAGACGGGTTCGTGA

Upstream 100 bases:

>100_bases
TTTTTACACGCCGAGCCGCCGTCGCTGCGGCGTGTGCTATAAAGCGGTCGTAAAAGGGACCCACAACCGGGCGAACCCGG
TCTACCAACACACACCTGTC

Downstream 100 bases:

>100_bases
CGGCCCGGCCGCCGGGCGCCGCTTGCGGCGCGCCCGGCTCCGGCACGCAGCCATAAAAAAACGCGGATCGATCGATCCGC
GTTTTTTCTTTGTGCCGGCT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDDFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIAQHAKAKQPVQRLWLQSMTPQAIRDGFANLRSDTDMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACASGFYEGKWYDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQIGTVSEESKPSTQLSPLLFDLTSLQREANGRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKALSEP
EQKLYDLVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLLREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACTKCDFSISKIPGSRQFEIPEVEELLQKKEIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLADGRTDLLPNFKSSRTGRNFK
AFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGAEEKPAPARKTAARKTTARKTGS

Sequences:

>Translated_865_residues
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDDFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAK
SESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIAQHAKAKQPVQRLWLQSMTPQAIRDGFANLRSDTDMQPLADAA
RCRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACASGFYEGKWYDP
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQIGTVSEESKPSTQLSPLLFDLTSLQREANGRFGFSAKNTLGLAQALYE
KHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKALSEP
EQKLYDLVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLLREGRELIPTAKAFQLMTLLR
GLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRR
FACTKCDFSISKIPGSRQFEIPEVEELLQKKEIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLADGRTDLLPNFKSSRTGRNFK
AFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGAEEKPAPARKTAARKTTARKTGS
>Mature_864_residues
SKALIIAEKPSVANDIARALGGFTKHDEYYESDDFVLSSAVGHLLEIAAPEEYEVKRGKWSFAHLPVIPPHFDLNPIAKS
ESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIAQHAKAKQPVQRLWLQSMTPQAIRDGFANLRSDTDMQPLADAAR
CRSEADWLVGINGTRAMTAFNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACASGFYEGKWYDPK
FKRDEFDPEKRDSRLWSLPAAETIVAACRDQIGTVSEESKPSTQLSPLLFDLTSLQREANGRFGFSAKNTLGLAQALYEK
HKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHAKQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKALSEPE
QKLYDLVVKRFLAVFFPAAEFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAVA
LVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLLREGRELIPTAKAFQLMTLLRG
LGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARMTQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRF
ACTKCDFSISKIPGSRQFEIPEVEELLQKKEIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAPD
FSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLADGRTDLLPNFKSSRTGRNFKA
FLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVTEGAEEKPAPARKTAARKTTARKTGS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=649, Percent_Identity=26.3482280431433, Blast_Score=147, Evalue=6e-35,
Organism=Homo sapiens, GI4507635, Length=700, Percent_Identity=23.4285714285714, Blast_Score=119, Evalue=1e-26,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=34.248788368336, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1787529, Length=669, Percent_Identity=26.0089686098655, Blast_Score=155, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=626, Percent_Identity=25.5591054313099, Blast_Score=129, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17555378, Length=634, Percent_Identity=23.8170347003155, Blast_Score=102, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323263, Length=662, Percent_Identity=25.8308157099698, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585251, Length=646, Percent_Identity=25.6965944272446, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24640096, Length=716, Percent_Identity=21.6480446927374, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 96481; Mature: 96350

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKALIIAEKPSVANDIARALGGFTKHDEYYESDDFVLSSAVGHLLEIAAPEEYEVKRGK
CCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIAQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFANLRSDTDMQPLADAARCRSEADWLVGINGTRAMTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACASGFYEGKWYDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQIGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKALSEPEQKLYDLVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCH
EFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLL
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACTKCDFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCC
IPEVEELLQKKEIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
CHHHHHHHHHHCCCCHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLLPNFKSSRTGRNFKAFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
EGAEEKPAPARKTAARKTTARKTGS
CCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKALIIAEKPSVANDIARALGGFTKHDEYYESDDFVLSSAVGHLLEIAAPEEYEVKRGK
CCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCC
WSFAHLPVIPPHFDLNPIAKSESRLKVLTKLMKRKDVDRLINACDAGREGELIFRLIAQH
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
AKAKQPVQRLWLQSMTPQAIRDGFANLRSDTDMQPLADAARCRSEADWLVGINGTRAMTA
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
FNSKGGGFFLTTVGRVQTPTLSIVVEREEKIRRFIPRDYWEVKAEFACASGFYEGKWYDP
ECCCCCCEEEEECCCCCCCEEEEEECCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
KFKRDEFDPEKRDSRLWSLPAAETIVAACRDQIGTVSEESKPSTQLSPLLFDLTSLQREA
CCCCCCCCCHHHCCCEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
NGRFGFSAKNTLGLAQALYEKHKVLTYPRTDARALPEDYLSTVQSTLEMLKESNNYLPHA
CCCCCCCCHHHHHHHHHHHHHHCEEECCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCHH
KQVLDKGWVKPNKRIFDNSKISDHFAIIPTLQAPKALSEPEQKLYDLVVKRFLAVFFPAA
HHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHHCCCH
EFRVTTRITEVAGHHFKTEGKVLVEPGWLQVYGRDAEGADANLVPVQKDEKVKTDEIAAV
HHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCCEECCCCCCCCCHHHEEEE
ALVTKPPARYSEATLLSAMEGAGKLVEDDELREAMAAKGLGTPATRAAIIEGLLGEKYLL
EEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHH
REGRELIPTAKAFQLMTLLRGLGVKELTAPELTGEWEYKLSQMERGNLGRDAFMQEIARM
HCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHH
TQQIVKRAKEYDSDTIPGDYATLETPCPNCGGQVKENYRRFACTKCDFSISKIPGSRQFE
HHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCC
IPEVEELLQKKEIGPLSGFRSKMGRPFSAILKLSFDDETKNYKLEFDFGQDQGGEEGEAP
CHHHHHHHHHHCCCCHHHHHHHHCCCHHHHEEEEECCCCCCEEEEEECCCCCCCCCCCCC
DFSAQEPVGACPKCKGRVFEHGMSYVCEHSVANPKTCDFRSGKVILQQEITREQMGKLLA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
DGRTDLLPNFKSSRTGRNFKAFLVKQPDGKIGFEFEKKEPKAAAAKKTAKSATKDAETVT
CCCHHCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHH
EGAEEKPAPARKTAARKTTARKTGS
CCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA