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The map label for this gene is purR [H]

Identifier: 116690990

GI number: 116690990

Start: 3286284

End: 3287303

Strand: Direct

Name: purR [H]

Synonym: Bcen2424_2972

Alternate gene names: 116690990

Gene position: 3286284-3287303 (Clockwise)

Preceding gene: 116690989

Following gene: 116690991

Centisome position: 94.33

GC content: 72.75

Gene sequence:

>1020_bases
ATGGGTACGACCATTCGCGATGTGGCGCGGGCAGCAGAGGTCTCGATCGGCACCGTCTCCCGCGCGCTGAAAAACCAGCC
CGGCCTGTCCGAAGCCACGCGTGCGCGCATCGTCGGGATCGCCCAGCAGCTCGGCTACGATCCCGCCCAGCTGCGGCCGC
GCATCCGCCGGCTCACCTTCCTGCTGCACCGCCAGCACAACCGCTTTCCCGCGAGCCCGTTCTTCTCGCACGTGCTGCAC
GGCGTCGAGGACGCGTGCCGCGAGCGCGGCATCGTGCCGACGCTGCTCACGGTCGGCCCGAACGACGACGTGCTGCGCCA
GATGCGCCCGCACGCGCCCGACGCGATCGCGGTCGCCGGCTTCATCGAGCCCGAGACGATCGAGGCGCTCGCCGCCACGG
GCCGCCCGCTCGTGCTGATCGACCTGTGGGCGCCCGGGCTGCGCTCGGTCAACATCGACAACGCGACGGGCGCGGCGCTC
GCGATGCGCCACCTGCTCGCCACCGGCCGCTCGCGGATCGCCTTCATCGGCGGCTCGGCCGCGCACTACAGCATCGCGCA
GCGCGCGATCGGCTACCGGCGCGCGTTCTTCGAAGCCGGGCGGCTGTTCGATCCCGCGTACGAAGTGACGATCGACGCGG
GGCTCGACCCGGACACGGGCGCCGCGCGTGCGATGGAGCAACTGCTCGACGCGCCCGGCCCGCGGCCCGAAGCCGTGTTC
GCGTACAACGACGCGGCCGCGCTGGCCGCGCAGCGCGTGTGCATCGCGCGCGGCCTGCGGATTCCGGACGACATCGCGAT
CGTCGGCTTCGACGACATCCCGGCCGCCGCGCACGCGAACCCGCCGCTCACGACGCTCGCGGTCGACAAAGAAGCGCTCG
GCCGCCGCGGCGTCGAGCTGCTGCTCGCCGACGCGCCCGAGCGCACCGAGATTTCCCTGCCCGTCGAGCTGATCGTGCGG
GCCAGCAGCCAGCCCGCCGGCTCCCCGGCACTCGATACCGTAACGGCCACCGAATCATGA

Upstream 100 bases:

>100_bases
AACCCGTTATTCGCGGGTATTCCCCGGTTATCCGGCCCGAATTCCGCCATCCGTTTTACTATACAATCGCTTTCAACCTA
AACAAACCGGACCCCGAACG

Downstream 100 bases:

>100_bases
ACATGCCCCCGGTCCAACCCTGCACGGCCGCGCCGGCCGCCCACACGCCCGCCGTACCGTTCGTCGCGAGCTTCCGCGAT
CCGTCGTTCCTGCTGTCGCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVGIAQQLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRSRIAFIGGSAAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGAARAMEQLLDAPGPRPEAVF
AYNDAAALAAQRVCIARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLADAPERTEISLPVELIVR
ASSQPAGSPALDTVTATES

Sequences:

>Translated_339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVGIAQQLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRSRIAFIGGSAAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGAARAMEQLLDAPGPRPEAVF
AYNDAAALAAQRVCIARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLADAPERTEISLPVELIVR
ASSQPAGSPALDTVTATES
>Mature_338_residues
GTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVGIAQQLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLHG
VEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALA
MRHLLATGRSRIAFIGGSAAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGAARAMEQLLDAPGPRPEAVFA
YNDAAALAAQRVCIARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVELLLADAPERTEISLPVELIVRA
SSQPAGSPALDTVTATES

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=352, Percent_Identity=31.8181818181818, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=32.9073482428115, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789456, Length=319, Percent_Identity=29.4670846394984, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=29.073482428115, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=29.9093655589124, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI48994940, Length=341, Percent_Identity=28.7390029325513, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.6666666666667, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789068, Length=298, Percent_Identity=26.1744966442953, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786540, Length=353, Percent_Identity=26.0623229461756, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787906, Length=269, Percent_Identity=28.6245353159851, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=24.1590214067278, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36148; Mature: 36016

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVGIAQQLGYDPAQLRPRIRRLTF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRSRIAFIGGSA
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGAARAMEQLLDAPGPRPEAVF
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCIARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHCCCCEEE
LLADAPERTEISLPVELIVRASSQPAGSPALDTVTATES
EEECCCCCCEECCCHHHEEECCCCCCCCCCCCCEECCCC
>Mature Secondary Structure 
GTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVGIAQQLGYDPAQLRPRIRRLTF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRSRIAFIGGSA
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCCEEEEECCCH
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGAARAMEQLLDAPGPRPEAVF
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCIARGLRIPDDIAIVGFDDIPAAAHANPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEECHHHHCCCCEEE
LLADAPERTEISLPVELIVRASSQPAGSPALDTVTATES
EEECCCCCCEECCCHHHEEECCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA