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The map label for this gene is yafC [C]

Identifier: 116690985

GI number: 116690985

Start: 3279374

End: 3280336

Strand: Reverse

Name: yafC [C]

Synonym: Bcen2424_2967

Alternate gene names: 116690985

Gene position: 3280336-3279374 (Counterclockwise)

Preceding gene: 116690986

Following gene: 116690984

Centisome position: 94.16

GC content: 67.81

Gene sequence:

>963_bases
ATGGATACCCTCGTCAGCATGAATGTGTTTCGCTACGTCGTCGAAGTGGGCAGCTTCGTCGGCGCGGCGGAGCGCATGCA
GATGTCGGCGGCGATGGCGAGCAAGCACGTGATGCACCTCGAGCAGCAGCTCGGCGCGCGGCTGCTGCATCGCACGACAC
GACGCGTCGCGCCGACCGAGGCGGGACGCGAATACTATGAGCGCCTCGTGCAGGCGCTGTCCGAACTCGACGAAGCCGGC
CAGGCCGTCGGCGCCGCGAGCGTGGTGCCGCAGGGGCGGTTGCGCGTGACGTCGCTGTCCGCGTTCGGGCTGCGGCACGT
GATGCAGGCCGTCACCGATTACGCGGGCCGGTTCCCGGACGTGACCGTCGACATGACGCTGTCCGATCGCGTGGTCGACC
TGATCGAGGAAGGCTACGACGTCGCGGTGCGCGCGGCGCCGAACGGATTGAAATCGTCGTCGCTGGTCGCGCGGCAGATC
GCGACCGCGCACATCCTGCTGGTCGCGTCGCCCGAGTATCTCGAGAAACACGGCACGCCGCAGACGATCGCCGATCTCGC
GAACCACAACTACCTGCGGCGCGATTCGAATTCGACGATGCTCGATTCGCTCGTGATCGACGCGGCCGCTGCTTCGCGCG
TGAGCCTGAACGGCAACCTGATCGTGAACCATCTCGAAGGGCTGCGCGCGGCCGTGCTCGCCGGCGCGGGCATCGCGCTG
CTCGGCACCGAAGTGGTCGGCGACGACATCGAATCGGGCCGGCTCGTGCCGTTGCTGCTCGATTCCGTGCCGCCGCACGA
GGCGCCGATCTATGCGGTGTACGCGAGCCGTCGCCACGTGTCCGCGAAGGTGCGCTCGTTCGTCGATTTCCTGGCCGCGC
GTTTCGAAGGGCAGTCGCTGTGCCCGTCGATCGAATCGCGGCTGCGCGTGCTGCCGATCACGCGGATGAAGCGCGCGGTC
TGA

Upstream 100 bases:

>100_bases
ACCCGCGGCGCCGTTTTGCCTTTCTTGACCTTTCTGTTTCCTCGCCTTTGTTACCCTATCTCGTAATTGGTTGATTCCCC
CGGGATTGCGAGCTAGTTCG

Downstream 100 bases:

>100_bases
GCCAAAGCGTCGGCCCGTCTGCCGCGGCGGGGTAATAAAAAAGCGCGAACTGCAACGGTTCGCGCTTAATAGGATGGTCA
GCCCGATCCTCACTCAGTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPDVTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQI
ATAHILLVASPEYLEKHGTPQTIADLANHNYLRRDSNSTMLDSLVIDAAAASRVSLNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIESGRLVPLLLDSVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRLRVLPITRMKRAV

Sequences:

>Translated_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPDVTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQI
ATAHILLVASPEYLEKHGTPQTIADLANHNYLRRDSNSTMLDSLVIDAAAASRVSLNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIESGRLVPLLLDSVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRLRVLPITRMKRAV
>Mature_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPDVTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQI
ATAHILLVASPEYLEKHGTPQTIADLANHNYLRRDSNSTMLDSLVIDAAAASRVSLNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIESGRLVPLLLDSVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRLRVLPITRMKRAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=35.3146853146853, Blast_Score=168, Evalue=4e-43,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=36.4548494983278, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=31.0810810810811, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=30.3448275862069, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=29.5918367346939, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=29.7658862876254, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=26.7558528428094, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=27.1812080536913, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788706, Length=200, Percent_Identity=26.5, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=22.4832214765101, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789204, Length=261, Percent_Identity=23.7547892720307, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34677; Mature: 34677

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALSELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
VTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQIATAHILLVASPEYLEKHGTP
EEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCH
QTIADLANHNYLRRDSNSTMLDSLVIDAAAASRVSLNGNLIVNHLEGLRAAVLAGAGIAL
HHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCEEECCCCEEEHHHHHHHHHHHHCCCHHH
LGTEVVGDDIESGRLVPLLLDSVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSL
HHHHHHCCCCCCCCEEEEHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
CPSIESRLRVLPITRMKRAV
CHHHHHCCEECCHHHHHHCC
>Mature Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALSELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
VTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQIATAHILLVASPEYLEKHGTP
EEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCH
QTIADLANHNYLRRDSNSTMLDSLVIDAAAASRVSLNGNLIVNHLEGLRAAVLAGAGIAL
HHHHHHHCCCCEECCCCCHHHHHHHHHHHHHCEEECCCCEEEHHHHHHHHHHHHCCCHHH
LGTEVVGDDIESGRLVPLLLDSVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSL
HHHHHHCCCCCCCCEEEEHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
CPSIESRLRVLPITRMKRAV
CHHHHHCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]