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The map label for this gene is ghrA [H]

Identifier: 116690976

GI number: 116690976

Start: 3271322

End: 3272263

Strand: Direct

Name: ghrA [H]

Synonym: Bcen2424_2958

Alternate gene names: 116690976

Gene position: 3271322-3272263 (Clockwise)

Preceding gene: 116690974

Following gene: 116690977

Centisome position: 93.9

GC content: 72.08

Gene sequence:

>942_bases
ATGAAGATCCTGTTCCATTCACCGCATCAGGACGCCGGCGCATGGCGCGACGAGATCGCGCGTGCACTGCCGGAAGCCGA
ACTGCGCGCCTGGCAGCCGGGCGATACGGCCGCCGCCGACTACGCGCTCGTGTGGCGCCCGCCGCGCGAGTTGTTCGCGC
CGCGCGACGGCCTGCGCGCGATCTTCAACCTCGGCGCGGGCGTCGATGCGCTGCTCGCGCTCGAGCACGCGCATCCGGGC
ACGCTGCCCGCGCACGTGCCGCTGGTGCGGCTCGAAGATTCCGGCATGGCGCAGCAGATGGTCGAATACGTGACGCACGC
GGCGCTGCGCTACCTGCGCCGTTTCGACGAATACGACCTGCAACAGCGCGAACGCCGCTGGCAGCCGCTCGAACCGCATG
CGCGCGCAAGCTTCACCGTCGCCGTCCTCGGCCTCGGCGTGCTTGGTGCGGCAGTCGCCCGCGCGCTCGCCGCGCTCGGC
CTGCCGGTGCGCGGCTACAGCCGCAGCGCGAAGCAGCTCGACGGCATCGCGACCTTCGCCGGCGACGGCGCGTTCGACGC
GTGCATCGACGGCGCGAAGGTGCTCGTCAACCTGCTGCCGAGCACGCGCGACACCGACGGCATCCTGTCGGCGCGCGCGT
TCGCGCGGCTCGCGCCCGGTGCATACGTCGTCAACGTCGCGCGCGGCGCGCATCTCGTCGAAGCCGACCTGCTCGACGCA
CTCGCGAGCGGCCAGATCGCCGCGGCCACGCTCGACGTGTTCCAGCACGAACCGCTGCCGGACGACCATCCGTTCTGGCG
CGCGCCGCGCATCACGATCACGCCGCACAGCTCGGCCGAGACGCTGCGCGCGGAAGCCGTCGAGCAGATCGCCGGCAAGA
TCCGCGCGTTCGAGCGCGGCGCGACCGTCAGCGGCATCGTCGACTACGCACGCGGCTACTGA

Upstream 100 bases:

>100_bases
TCAGGAGTCGCCCGATGTCCGCTACGTCCGCCGGCGCGCCGCGCCCGGCTCTACTCGTCGTTCCCGCGACGCACCTCGCC
GGCGCGCGCGCCGTCACGCA

Downstream 100 bases:

>100_bases
ATTCCGCCTACACAAAACCGGAGACAACCATGACGTTCCCCGCCACCGTCAAGATCGTCGAAGTCGGCCCGCGCGACGGG
CTGCAGAACGAAAAGACCTT

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKILFHSPHQDAGAWRDEIARALPEAELRAWQPGDTAAADYALVWRPPRELFAPRDGLRAIFNLGAGVDALLALEHAHPG
TLPAHVPLVRLEDSGMAQQMVEYVTHAALRYLRRFDEYDLQQRERRWQPLEPHARASFTVAVLGLGVLGAAVARALAALG
LPVRGYSRSAKQLDGIATFAGDGAFDACIDGAKVLVNLLPSTRDTDGILSARAFARLAPGAYVVNVARGAHLVEADLLDA
LASGQIAAATLDVFQHEPLPDDHPFWRAPRITITPHSSAETLRAEAVEQIAGKIRAFERGATVSGIVDYARGY

Sequences:

>Translated_313_residues
MKILFHSPHQDAGAWRDEIARALPEAELRAWQPGDTAAADYALVWRPPRELFAPRDGLRAIFNLGAGVDALLALEHAHPG
TLPAHVPLVRLEDSGMAQQMVEYVTHAALRYLRRFDEYDLQQRERRWQPLEPHARASFTVAVLGLGVLGAAVARALAALG
LPVRGYSRSAKQLDGIATFAGDGAFDACIDGAKVLVNLLPSTRDTDGILSARAFARLAPGAYVVNVARGAHLVEADLLDA
LASGQIAAATLDVFQHEPLPDDHPFWRAPRITITPHSSAETLRAEAVEQIAGKIRAFERGATVSGIVDYARGY
>Mature_313_residues
MKILFHSPHQDAGAWRDEIARALPEAELRAWQPGDTAAADYALVWRPPRELFAPRDGLRAIFNLGAGVDALLALEHAHPG
TLPAHVPLVRLEDSGMAQQMVEYVTHAALRYLRRFDEYDLQQRERRWQPLEPHARASFTVAVLGLGVLGAAVARALAALG
LPVRGYSRSAKQLDGIATFAGDGAFDACIDGAKVLVNLLPSTRDTDGILSARAFARLAPGAYVVNVARGAHLVEADLLDA
LASGQIAAATLDVFQHEPLPDDHPFWRAPRITITPHSSAETLRAEAVEQIAGKIRAFERGATVSGIVDYARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=28.6919831223629, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI23308577, Length=267, Percent_Identity=28.0898876404494, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=48.8817891373802, Blast_Score=308, Evalue=2e-85,
Organism=Escherichia coli, GI87082289, Length=156, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=172, Percent_Identity=29.6511627906977, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322116, Length=160, Percent_Identity=28.75, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=160, Percent_Identity=28.75, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24585516, Length=277, Percent_Identity=25.9927797833935, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24585514, Length=238, Percent_Identity=27.7310924369748, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574282, Length=238, Percent_Identity=27.7310924369748, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI28574284, Length=238, Percent_Identity=27.7310924369748, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45552429, Length=238, Percent_Identity=27.7310924369748, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551003, Length=238, Percent_Identity=27.7310924369748, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574286, Length=180, Percent_Identity=30.5555555555556, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921140, Length=152, Percent_Identity=32.8947368421053, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571528, Length=138, Percent_Identity=31.1594202898551, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33879; Mature: 33879

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFHSPHQDAGAWRDEIARALPEAELRAWQPGDTAAADYALVWRPPRELFAPRDGLRA
CEEEECCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEECCHHHHHCCCHHHHH
IFNLGAGVDALLALEHAHPGTLPAHVPLVRLEDSGMAQQMVEYVTHAALRYLRRFDEYDL
HHHCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCH
QQRERRWQPLEPHARASFTVAVLGLGVLGAAVARALAALGLPVRGYSRSAKQLDGIATFA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHEEC
GDGAFDACIDGAKVLVNLLPSTRDTDGILSARAFARLAPGAYVVNVARGAHLVEADLLDA
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
LASGQIAAATLDVFQHEPLPDDHPFWRAPRITITPHSSAETLRAEAVEQIAGKIRAFERG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
ATVSGIVDYARGY
CHHHHHHHHHCCC
>Mature Secondary Structure
MKILFHSPHQDAGAWRDEIARALPEAELRAWQPGDTAAADYALVWRPPRELFAPRDGLRA
CEEEECCCCCCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEECCHHHHHCCCHHHHH
IFNLGAGVDALLALEHAHPGTLPAHVPLVRLEDSGMAQQMVEYVTHAALRYLRRFDEYDL
HHHCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCH
QQRERRWQPLEPHARASFTVAVLGLGVLGAAVARALAALGLPVRGYSRSAKQLDGIATFA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHEEC
GDGAFDACIDGAKVLVNLLPSTRDTDGILSARAFARLAPGAYVVNVARGAHLVEADLLDA
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
LASGQIAAATLDVFQHEPLPDDHPFWRAPRITITPHSSAETLRAEAVEQIAGKIRAFERG
HHCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
ATVSGIVDYARGY
CHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA