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The map label for this gene is phbI [H]

Identifier: 116690880

GI number: 116690880

Start: 3158555

End: 3160333

Strand: Reverse

Name: phbI [H]

Synonym: Bcen2424_2860

Alternate gene names: 116690880

Gene position: 3160333-3158555 (Counterclockwise)

Preceding gene: 116690881

Following gene: 116690876

Centisome position: 90.71

GC content: 67.4

Gene sequence:

>1779_bases
GTGCGCGTGTCCTTCACGCTGCATGGCATTCCCGTCTCACGAGGTATCGCGATCGGGCGAGCGTATCTGATCGCGCCGGC
GGCGCTTGACGTCGCCCATTACCTGATCGAGGCCAACCGGACCGACGCCGAAGTGGAGCGATTCCGCACGGCGCTCGACG
TCGTGCGCCGCGAGCTCCAAGCGCTGCGCGCCGACCTGACCGACGATACCCCGAGCGAAGTCGGTGCATTCATCGACGTC
CACACGATGATCCTGAGCGACGAGATGCTCGTGCAGGAAACCATCGACCTGATCCGCACGCGCCGCTACAACGTCGAATG
GGCGCTGACCGAGCAGCTCGAACTGCTGACGCGCCATTTCGACGACATCGAGGACGAATACCTGCGCGAGCGCAAGGCCG
ACATCGAGCAGGTGGTCGAGCGCGTGCTGAAGGCGCTCGCCGGTGCGCCGTCCGCATCGCAGGCGCTCGACGGCGCGGCC
AGGAACGGCACGAACGACATGATCGTCGTCGCGCACGACATCGCGCCGGCCGACATGATGCAGTTCAAGACGCAGTCGTT
CCAGGCGTTCGTCACCGATCTCGGCGGGCGCACGTCGCACACGGCGATCGTCGCGCGCAGCCTCGGCATTCCGGCCGCGG
TCGGCGTGCAGCATGCGAGTTCGCTGATCCGCCAGGACGACCTGATCATCGTCGACGGCGACCAGGGCATCGTGATCGTC
GATCCGGCGCCGATCGTCCTCGAGGAATATTCGTACCGCCAGTCCGAGAAGCTGCTGGAGCAACGCAAGCTGCAGCGCCT
GAAGTTCTCGCCGACGCAAACGCTGTGCGGCACGAAGATCGACCTGTACGCGAACATCGAGCTGCCCGACGACGCGAAGG
CGGCCGTCGAGGCCGGCGCGGTCGGCGTCGGCCTGTTCCGCTCCGAGTTCCTGTTCATGCACGAGAAGGAGATGCCGGAA
GAGGAGGAGCAGTTCGCCGCGTACAAGCGGGCTGTCGAGTGGATGAAGGGCATGCCGGTGACGATCCGCACGATCGACGT
CGGCGCCGACAAACCGCTCGAGGCGCTCGACGAAGGCTACGAAACGGCGCCGAACCCGGCGCTCGGGCTGCGCGCGATCC
GCTGGAGCCTGTCCGAGCCGCAGATGTTCCTCACGCAGTTGCGCGCGATCCTGCGCGCGTCCGCGTTCGGCCAGGTGAAG
ATCCTGATCCCGATGCTCGCGCACGCGCAGGAGATCGACCAGACGCTCGACCTGATCCGCGAGGCGAAGCGGCAGCTCGA
CGACGCGGGGCTCGCATACGATCCGAACGTGCGCCTCGGCGCAATGATCGAGATCCCGGCCGCGGCAATCGCGCTGCCGC
TGTTCCTGAAGCGCTTCGATTTCCTGTCGATCGGCACGAACGACCTGATCCAGTACACGCTCGCGATCGATCGCGCCGAC
AACGCGGTCGCGCACCTGTACGACCCGCTGCATCCGGCCGTGCTGCACCTGATCGCGTACACCTTGCGCGAAGCGAAGCG
CGCGGGCGTGTCGGTGTCGGTGTGCGGGGAGATGGCGGGCGACCCGGCGCTCACGCGCCTGTTGCTCGGGATGGGGCTCA
CCGAGTTCTCGATGCACCCGAGCCAGTTGCTCGTCGTGAAGCAGGAGATCCTGCGCGCGCACCTGAAGGCGCTCGAGAAG
CCGACGGCCGACGTGCTGGCCGCGTTCGAGCCGGAGGAAGTGCAGGCGGCGCTGCAGCGGCTGGCGGTCGCCGAGCCGCG
GGCGGACGCGGCCGCGTAG

Upstream 100 bases:

>100_bases
CGCTGCATGCAGTCAGCAGGGAGTCGCGGAATTTATAATCGCTAACCGGGGTCATAAGCGCATCGCAGCAGTGTGCCGCG
GCTTTACTTGTACAGAGGAG

Downstream 100 bases:

>100_bases
GCGTTTTCGGCGCCGCTAACTGTAGCGAAGTGTCACGCATGCGCGTTCGGCGCATGCCCGTCGCGCGTCAGTGCCGGCCC
CCGCATACGGGGCAGTCGGC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRTDAEVERFRTALDVVRRELQALRADLTDDTPSEVGAFIDV
HTMILSDEMLVQETIDLIRTRRYNVEWALTEQLELLTRHFDDIEDEYLRERKADIEQVVERVLKALAGAPSASQALDGAA
RNGTNDMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASSLIRQDDLIIVDGDQGIVIV
DPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTKIDLYANIELPDDAKAAVEAGAVGVGLFRSEFLFMHEKEMPE
EEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVK
ILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRLGAMIEIPAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRAD
NAVAHLYDPLHPAVLHLIAYTLREAKRAGVSVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEK
PTADVLAAFEPEEVQAALQRLAVAEPRADAAA

Sequences:

>Translated_592_residues
MRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRTDAEVERFRTALDVVRRELQALRADLTDDTPSEVGAFIDV
HTMILSDEMLVQETIDLIRTRRYNVEWALTEQLELLTRHFDDIEDEYLRERKADIEQVVERVLKALAGAPSASQALDGAA
RNGTNDMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASSLIRQDDLIIVDGDQGIVIV
DPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTKIDLYANIELPDDAKAAVEAGAVGVGLFRSEFLFMHEKEMPE
EEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVK
ILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRLGAMIEIPAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRAD
NAVAHLYDPLHPAVLHLIAYTLREAKRAGVSVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEK
PTADVLAAFEPEEVQAALQRLAVAEPRADAAA
>Mature_592_residues
MRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRTDAEVERFRTALDVVRRELQALRADLTDDTPSEVGAFIDV
HTMILSDEMLVQETIDLIRTRRYNVEWALTEQLELLTRHFDDIEDEYLRERKADIEQVVERVLKALAGAPSASQALDGAA
RNGTNDMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASSLIRQDDLIIVDGDQGIVIV
DPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTKIDLYANIELPDDAKAAVEAGAVGVGLFRSEFLFMHEKEMPE
EEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVK
ILIPMLAHAQEIDQTLDLIREAKRQLDDAGLAYDPNVRLGAMIEIPAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRAD
NAVAHLYDPLHPAVLHLIAYTLREAKRAGVSVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLKALEK
PTADVLAAFEPEEVQAALQRLAVAEPRADAAA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=568, Percent_Identity=38.2042253521127, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=34.2644320297952, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI48994992, Length=529, Percent_Identity=32.1361058601134, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI1788726, Length=577, Percent_Identity=28.9428076256499, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1787994, Length=411, Percent_Identity=28.4671532846715, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI226510935, Length=203, Percent_Identity=24.6305418719212, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65341; Mature: 65341

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRTDAEVERFRTALDVVRRELQ
CEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ALRADLTDDTPSEVGAFIDVHTMILSDEMLVQETIDLIRTRRYNVEWALTEQLELLTRHF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
DDIEDEYLRERKADIEQVVERVLKALAGAPSASQALDGAARNGTNDMIVVAHDIAPADMM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCCEEEEEECCCCHHHH
QFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASSLIRQDDLIIVDGDQGIVIV
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEE
DPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTKIDLYANIELPDDAKAAVEAGA
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEEECCCCCHHHHHHHCC
VGVGLFRSEFLFMHEKEMPEEEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGY
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCC
ETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIR
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAKRQLDDAGLAYDPNVRLGAMIEIPAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRAD
HHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEHEEECCC
NAVAHLYDPLHPAVLHLIAYTLREAKRAGVSVSVCGEMAGDPALTRLLLGMGLTEFSMHP
CHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHHHHCCCHHHCCCC
SQLLVVKQEILRAHLKALEKPTADVLAAFEPEEVQAALQRLAVAEPRADAAA
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEANRTDAEVERFRTALDVVRRELQ
CEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ALRADLTDDTPSEVGAFIDVHTMILSDEMLVQETIDLIRTRRYNVEWALTEQLELLTRHF
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
DDIEDEYLRERKADIEQVVERVLKALAGAPSASQALDGAARNGTNDMIVVAHDIAPADMM
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHCCCCCCEEEEEECCCCHHHH
QFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASSLIRQDDLIIVDGDQGIVIV
HHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEE
DPAPIVLEEYSYRQSEKLLEQRKLQRLKFSPTQTLCGTKIDLYANIELPDDAKAAVEAGA
CCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCEEEEEEEECCCCCHHHHHHHCC
VGVGLFRSEFLFMHEKEMPEEEEQFAAYKRAVEWMKGMPVTIRTIDVGADKPLEALDEGY
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCC
ETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILIPMLAHAQEIDQTLDLIR
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAKRQLDDAGLAYDPNVRLGAMIEIPAAAIALPLFLKRFDFLSIGTNDLIQYTLAIDRAD
HHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEHEEECCC
NAVAHLYDPLHPAVLHLIAYTLREAKRAGVSVSVCGEMAGDPALTRLLLGMGLTEFSMHP
CHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCHHHHHHHHHCCCHHHCCCC
SQLLVVKQEILRAHLKALEKPTADVLAAFEPEEVQAALQRLAVAEPRADAAA
HHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]