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The map label for this gene is thcD [H]

Identifier: 116690700

GI number: 116690700

Start: 2969735

End: 2971003

Strand: Reverse

Name: thcD [H]

Synonym: Bcen2424_2680

Alternate gene names: 116690700

Gene position: 2971003-2969735 (Counterclockwise)

Preceding gene: 116690701

Following gene: 116690696

Centisome position: 85.28

GC content: 70.69

Gene sequence:

>1269_bases
ATGACCAGCGACCGGGTGATGGCGATCGTCGGCGCAGGTCATGCCGGCGGCCGCGCGGCGCAGGTGCTGCGCGCGTCCGG
CTGGCGCGGGCGTATCGTGCTGATCGGCGCGGAGCCGCATCCGCCGTACGAGCGGCCGCCGTTGTCCAAGGGCGTGCTGA
CGGGCGAGCGCAGCGCGGTGCAATGCCGGTTGCGCGACGCCGACGCATGGGCGGCTGATCGTATCGAGCGGATCGTCGCG
ACCGTCGAGCGCATCGATCCGCACGCACGCGAAGTGCGCATGTCCGGCGGACACGTGTTCGGCTACGACGCGCTGCTGCT
GGCCACCGGCGGCCGTGCACGCCGTCTGGCCATTCCGGGCGCGGCGCTCGACGGCGTAGTCGCGTTGCGCACGCTCGACG
ATGCGGCCGTACTCGGCGCGCGGCTCGTGCCCGACGCGCGGATCGTGCTGATCGGCGGCGGTTTCATCGGCCTCGAAGTC
GCGGCGTCGGCGCGTCGGCGCGGCTGCCGCGTGACCGTGCTCGATGCGGCACCGCGGTTGCTCGGCCGTGCGGTGCCGGA
AGCCATCGCGACGCGCGTGCATGCGCTGCACGATGCGCAGGGCGTGGCGATTCGCCTGAACCGAAAGCCGGTTGCGATCG
AGCGGACGTCGGGCGGTGCGCTGGCCGTCGTGCTCGACGACGGCGACACGCTGCTCGCCGACACGGTCGTGGCCGGTATC
GGCATCGAACCGGCCGATGAACTGGCACGCGATGCGGGCCTCGCGGTCGAGCGCGGCATCGTCGTGAATGCGCAGCTCGA
GACGTCGGCGCGCGGCATCTACGCGGCCGGCGACGTCGCGGTGTTTCCGAGCGCGTTATCGGGCCAGCTGGTTCGACAGG
AGACCTGGCACGGCGCCGAGACGCAAGCGCACGTCGCCGCCCGCAACATGCTCGGTGCGGCCGAAGCGTATCGCGACATG
CCGTGGTTCTGGTCGGATCAATACGATGCGCAGTTGCAGGTGGCCGGGGAGCCGGCGCTCGGTACGCAGTCGGTCGCACG
CAGCCTTGGCGACGATGCGGAAATCCATTTCCATTTCGATGCGCTCGCGCGGCTCGTCGCGGTGAGCGGCTTCGGGCGGG
CGGCGGGATTCGTGAAGGAAATGCGCGTGGCGAGGATGCTGGTCGAACGCGGGATCGACGTGACGCCGGCGGCGGTCGCG
GACGTCGACGTGAAGCTGAAATCGCTGGTGACGGCCGTGAATGACCGGCAGAAGAAAGGAGGGGAGTAA

Upstream 100 bases:

>100_bases
GTGCGCGACGGCCGGCCGCTGTCGGCGCCCGCGTGCGAGAAGCTGCGCACGTACCGCGCGAAGATCGAGGACGGCCGCAT
CCTGATCGAGGTATGACGCG

Downstream 100 bases:

>100_bases
AGCGCGCGGCAGCGAACGCCGCGCGCTTCGTTCAAACCGCGATCAGGCCATCGCGTGCAGGCGCAGATACAGGCCGGCTG
CCGCCGCGCCGCCGAGCAGC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTSDRVMAIVGAGHAGGRAAQVLRASGWRGRIVLIGAEPHPPYERPPLSKGVLTGERSAVQCRLRDADAWAADRIERIVA
TVERIDPHAREVRMSGGHVFGYDALLLATGGRARRLAIPGAALDGVVALRTLDDAAVLGARLVPDARIVLIGGGFIGLEV
AASARRRGCRVTVLDAAPRLLGRAVPEAIATRVHALHDAQGVAIRLNRKPVAIERTSGGALAVVLDDGDTLLADTVVAGI
GIEPADELARDAGLAVERGIVVNAQLETSARGIYAAGDVAVFPSALSGQLVRQETWHGAETQAHVAARNMLGAAEAYRDM
PWFWSDQYDAQLQVAGEPALGTQSVARSLGDDAEIHFHFDALARLVAVSGFGRAAGFVKEMRVARMLVERGIDVTPAAVA
DVDVKLKSLVTAVNDRQKKGGE

Sequences:

>Translated_422_residues
MTSDRVMAIVGAGHAGGRAAQVLRASGWRGRIVLIGAEPHPPYERPPLSKGVLTGERSAVQCRLRDADAWAADRIERIVA
TVERIDPHAREVRMSGGHVFGYDALLLATGGRARRLAIPGAALDGVVALRTLDDAAVLGARLVPDARIVLIGGGFIGLEV
AASARRRGCRVTVLDAAPRLLGRAVPEAIATRVHALHDAQGVAIRLNRKPVAIERTSGGALAVVLDDGDTLLADTVVAGI
GIEPADELARDAGLAVERGIVVNAQLETSARGIYAAGDVAVFPSALSGQLVRQETWHGAETQAHVAARNMLGAAEAYRDM
PWFWSDQYDAQLQVAGEPALGTQSVARSLGDDAEIHFHFDALARLVAVSGFGRAAGFVKEMRVARMLVERGIDVTPAAVA
DVDVKLKSLVTAVNDRQKKGGE
>Mature_421_residues
TSDRVMAIVGAGHAGGRAAQVLRASGWRGRIVLIGAEPHPPYERPPLSKGVLTGERSAVQCRLRDADAWAADRIERIVAT
VERIDPHAREVRMSGGHVFGYDALLLATGGRARRLAIPGAALDGVVALRTLDDAAVLGARLVPDARIVLIGGGFIGLEVA
ASARRRGCRVTVLDAAPRLLGRAVPEAIATRVHALHDAQGVAIRLNRKPVAIERTSGGALAVVLDDGDTLLADTVVAGIG
IEPADELARDAGLAVERGIVVNAQLETSARGIYAAGDVAVFPSALSGQLVRQETWHGAETQAHVAARNMLGAAEAYRDMP
WFWSDQYDAQLQVAGEPALGTQSVARSLGDDAEIHFHFDALARLVAVSGFGRAAGFVKEMRVARMLVERGIDVTPAAVAD
VDVKLKSLVTAVNDRQKKGGE

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=339, Percent_Identity=31.5634218289086, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI65787454, Length=339, Percent_Identity=31.5634218289086, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI226437568, Length=339, Percent_Identity=31.5634218289086, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI4757732, Length=334, Percent_Identity=29.6407185628743, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI22202629, Length=334, Percent_Identity=29.6407185628743, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI22202631, Length=169, Percent_Identity=33.1360946745562, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788892, Length=411, Percent_Identity=35.5231143552311, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789065, Length=307, Percent_Identity=29.9674267100977, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789915, Length=193, Percent_Identity=29.0155440414508, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789765, Length=320, Percent_Identity=25.9375, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=364, Percent_Identity=28.021978021978, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24639257, Length=374, Percent_Identity=28.3422459893048, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281359715, Length=374, Percent_Identity=28.3422459893048, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281359713, Length=374, Percent_Identity=28.3422459893048, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639250, Length=374, Percent_Identity=28.3422459893048, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI18543267, Length=374, Percent_Identity=28.3422459893048, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639252, Length=374, Percent_Identity=28.3422459893048, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24585130, Length=375, Percent_Identity=25.8666666666667, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28573993, Length=388, Percent_Identity=28.0927835051546, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581020, Length=388, Percent_Identity=28.0927835051546, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44452; Mature: 44321

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDRVMAIVGAGHAGGRAAQVLRASGWRGRIVLIGAEPHPPYERPPLSKGVLTGERSAV
CCCCEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
QCRLRDADAWAADRIERIVATVERIDPHAREVRMSGGHVFGYDALLLATGGRARRLAIPG
EEEEECCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCEEEECEEEEEECCCCEEEEECCC
AALDGVVALRTLDDAAVLGARLVPDARIVLIGGGFIGLEVAASARRRGCRVTVLDAAPRL
CHHCCEEEEEECCHHHHHHHEECCCCEEEEEECCEEEHHHHHHHHHCCCEEEEECCCHHH
LGRAVPEAIATRVHALHDAQGVAIRLNRKPVAIERTSGGALAVVLDDGDTLLADTVVAGI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEECCCCEEEHHHHHHCC
GIEPADELARDAGLAVERGIVVNAQLETSARGIYAAGDVAVFPSALSGQLVRQETWHGAE
CCCCHHHHHHHCCCEEECCEEEEEEECCCCCCEEECCCCEECCCHHCCCHHHHHCCCCCC
TQAHVAARNMLGAAEAYRDMPWFWSDQYDAQLQVAGEPALGTQSVARSLGDDAEIHFHFD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEHH
ALARLVAVSGFGRAAGFVKEMRVARMLVERGIDVTPAAVADVDVKLKSLVTAVNDRQKKG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GE
CC
>Mature Secondary Structure 
TSDRVMAIVGAGHAGGRAAQVLRASGWRGRIVLIGAEPHPPYERPPLSKGVLTGERSAV
CCCEEEEEEECCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
QCRLRDADAWAADRIERIVATVERIDPHAREVRMSGGHVFGYDALLLATGGRARRLAIPG
EEEEECCCCHHHHHHHHHHHHHHHCCCCHHHEEECCCEEEECEEEEEECCCCEEEEECCC
AALDGVVALRTLDDAAVLGARLVPDARIVLIGGGFIGLEVAASARRRGCRVTVLDAAPRL
CHHCCEEEEEECCHHHHHHHEECCCCEEEEEECCEEEHHHHHHHHHCCCEEEEECCCHHH
LGRAVPEAIATRVHALHDAQGVAIRLNRKPVAIERTSGGALAVVLDDGDTLLADTVVAGI
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEECCCCEEEHHHHHHCC
GIEPADELARDAGLAVERGIVVNAQLETSARGIYAAGDVAVFPSALSGQLVRQETWHGAE
CCCCHHHHHHHCCCEEECCEEEEEEECCCCCCEEECCCCEECCCHHCCCHHHHHCCCCCC
TQAHVAARNMLGAAEAYRDMPWFWSDQYDAQLQVAGEPALGTQSVARSLGDDAEIHFHFD
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCEEEEEHH
ALARLVAVSGFGRAAGFVKEMRVARMLVERGIDVTPAAVADVDVKLKSLVTAVNDRQKKG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]