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The map label for this gene is purK [H]

Identifier: 116690643

GI number: 116690643

Start: 2906243

End: 2907439

Strand: Reverse

Name: purK [H]

Synonym: Bcen2424_2623

Alternate gene names: 116690643

Gene position: 2907439-2906243 (Counterclockwise)

Preceding gene: 116690644

Following gene: 116690642

Centisome position: 83.45

GC content: 71.6

Gene sequence:

>1197_bases
ATGACCGCAACTCCTGATTCCGTTTCCCCGATCCTGCCCGGCGCGTGGCTGGGCATGGTCGGCGGCGGCCAGCTCGGCCG
CATGTTCTGCTTTGCCGCCCAGTCGATGGGCTATCGCGTCGCCGTGCTCGATCCCGATCCGGCGAGCCCCGCCGGCGCGG
TCGCCGATCGGCACCTGCGCGCGGCGTACGACGACGAAGCCGCGCTTGCCGAACTGGCCGACCTGTGCGAGGCCGTATCG
ACGGAGTTCGAGAACGTGCCGGCCGCGAGCCTCGATTTCCTCGCGCGCACGACGTTCGTCGCCCCGGCTGGCCGCTGCGT
CGCGGTCGCGCAGGACCGGATCGCGGAGAAGCGTTTCATCGAGGCGTCGGGCGTGCCGGTCGCGCCGCACGTCGTGATCG
AATCGGCGGCGGCGCTTGCCGCGCTCGACGATGCGGCGCTTGACGCGGTGCTGCCGGGCATCCTGAAGACGGCCCGCCTC
GGTTACGACGGCAAGGGCCAGGTGCGCGTCGCCACCGCGCAGGAAGCACGCGACGCGCATGCCGCGCTCGGCGGCGTGCC
GTGCGTGCTGGAGAAGCGCCTGCCGCTGAAATACGAAGTGTCGGCGCTGATCGCGCGCGGCGCGGACGGCCGCTCGGCTG
CGTTCCCGCTCGCGCAGAACGTGCACCACAACGGCATCCTCGCGCTGACGGTCGTACCGGCGCCGGCCGCCGATGCGGCC
CGCGTCGAGGAAGCGCAGCAGGCGGCGATCCGGATCGCCGATACGCTCGGCTACGTCGGCGTGCTGTGCGTCGAGTTCTT
CGTGCTGGAGGACGGCTCGTTCGTCGCGAACGAAATGGCGCCGCGCCCGCACAACTCCGGCCACTACACGGTCGATGCGT
GCGCAACGAGCCAGTTCGAGCAGCAGGTGCGCGCGATGACGCGCATGCCGCTCGGCAATCCGCGCCAGCATTCGCCGGCC
GCGATGCTGAACATCCTCGGCGACGTGTGGTTCCCCAACGGGGCGGCGGCTGACGCCGTCACGCCGCCGTGGGACACGGT
CGCCGCGATGCCGGCTGCCCACCTGCACCTGTACGGCAAGGAAGAAGCGCGCGTCGGCCGCAAGATGGGCCACGTGAACT
TCACGGCCGAGACGCGCGACGAAGCCGTCGCGGCGGCCACCGCATGCGCGCAGCTGCTGCGCGTGCCGCTCGACTGA

Upstream 100 bases:

>100_bases
GCTGGAATGACGGCATGGCGCGCGGCCGCACGACGCGGCCGGCGCCGGATTTTCCCACTGCGGCGGGCCGTTCCGCGTTC
CGCCGCAACCGACCACTGAC

Downstream 100 bases:

>100_bases
GGCGCGGGCCGATGTCCATCGATCTCCCGACGCACGTGACGCCCGCGCAAATCGACGCGGCCGCCGCGCTGCTCGATGCG
GGCCAGCTCGTCGCGTTTCC

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALAELADLCEAVS
TEFENVPAASLDFLARTTFVAPAGRCVAVAQDRIAEKRFIEASGVPVAPHVVIESAAALAALDDAALDAVLPGILKTARL
GYDGKGQVRVATAQEARDAHAALGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNVHHNGILALTVVPAPAADAA
RVEEAQQAAIRIADTLGYVGVLCVEFFVLEDGSFVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQHSPA
AMLNILGDVWFPNGAAADAVTPPWDTVAAMPAAHLHLYGKEEARVGRKMGHVNFTAETRDEAVAAATACAQLLRVPLD

Sequences:

>Translated_398_residues
MTATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALAELADLCEAVS
TEFENVPAASLDFLARTTFVAPAGRCVAVAQDRIAEKRFIEASGVPVAPHVVIESAAALAALDDAALDAVLPGILKTARL
GYDGKGQVRVATAQEARDAHAALGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNVHHNGILALTVVPAPAADAA
RVEEAQQAAIRIADTLGYVGVLCVEFFVLEDGSFVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQHSPA
AMLNILGDVWFPNGAAADAVTPPWDTVAAMPAAHLHLYGKEEARVGRKMGHVNFTAETRDEAVAAATACAQLLRVPLD
>Mature_397_residues
TATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLRAAYDDEAALAELADLCEAVST
EFENVPAASLDFLARTTFVAPAGRCVAVAQDRIAEKRFIEASGVPVAPHVVIESAAALAALDDAALDAVLPGILKTARLG
YDGKGQVRVATAQEARDAHAALGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNVHHNGILALTVVPAPAADAAR
VEEAQQAAIRIADTLGYVGVLCVEFFVLEDGSFVANEMAPRPHNSGHYTVDACATSQFEQQVRAMTRMPLGNPRQHSPAA
MLNILGDVWFPNGAAADAVTPPWDTVAAMPAAHLHLYGKEEARVGRKMGHVNFTAETRDEAVAAATACAQLLRVPLD

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=378, Percent_Identity=33.0687830687831, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1788155, Length=389, Percent_Identity=26.9922879177378, Blast_Score=89, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=377, Percent_Identity=31.5649867374005, Blast_Score=198, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 41650; Mature: 41519

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLR
CCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AAYDDEAALAELADLCEAVSTEFENVPAASLDFLARTTFVAPAGRCVAVAQDRIAEKRFI
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
EASGVPVAPHVVIESAAALAALDDAALDAVLPGILKTARLGYDGKGQVRVATAQEARDAH
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
AALGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNVHHNGILALTVVPAPAADAA
HHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCHHH
RVEEAQQAAIRIADTLGYVGVLCVEFFVLEDGSFVANEMAPRPHNSGHYTVDACATSQFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEHHHHHHHH
QQVRAMTRMPLGNPRQHSPAAMLNILGDVWFPNGAAADAVTPPWDTVAAMPAAHLHLYGK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCHHHEEEECC
EEARVGRKMGHVNFTAETRDEAVAAATACAQLLRVPLD
HHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TATPDSVSPILPGAWLGMVGGGQLGRMFCFAAQSMGYRVAVLDPDPASPAGAVADRHLR
CCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
AAYDDEAALAELADLCEAVSTEFENVPAASLDFLARTTFVAPAGRCVAVAQDRIAEKRFI
HHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHH
EASGVPVAPHVVIESAAALAALDDAALDAVLPGILKTARLGYDGKGQVRVATAQEARDAH
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHH
AALGGVPCVLEKRLPLKYEVSALIARGADGRSAAFPLAQNVHHNGILALTVVPAPAADAA
HHHCCCCHHHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCHHH
RVEEAQQAAIRIADTLGYVGVLCVEFFVLEDGSFVANEMAPRPHNSGHYTVDACATSQFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCEEEEHHHHHHHH
QQVRAMTRMPLGNPRQHSPAAMLNILGDVWFPNGAAADAVTPPWDTVAAMPAAHLHLYGK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCHHHEEEECC
EEARVGRKMGHVNFTAETRDEAVAAATACAQLLRVPLD
HHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]