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The map label for this gene is oprM [H]

Identifier: 116690627

GI number: 116690627

Start: 2886532

End: 2888055

Strand: Reverse

Name: oprM [H]

Synonym: Bcen2424_2607

Alternate gene names: 116690627

Gene position: 2888055-2886532 (Counterclockwise)

Preceding gene: 116690628

Following gene: 116690625

Centisome position: 82.9

GC content: 68.24

Gene sequence:

>1524_bases
ATGATGCAAAAACACGCTTTGACTGCAATCGCAGTCGCGCTCTTTGCCACGGGCTGCACGATGGCGCCGCACTACAAGCG
GCCCGACGCACCCGTCGCGCAGGCGTACCCGGCCGGCGGCGTCTACGCGACGCAGCCGGGCGCAGCCGGCGCGCGCAGCG
CGAACGGCCAGGCGGCGACTGCCATCGGCTGGCGCGAGTTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATCGCG
CTGAAAAACAACCGCGACCTGCGCGTGTCGGTGCTCAATATCGAGGCGGCGCGTGCTCAGTACCAGATCACGCGTGCGGG
GCTGTTCCCGACGCTCGACGGTACGGGCACGGGCAACATCCAGCGTCTGCCGGCCGGTGTGTCGACGACGAGGGCGCCGC
TGATTTCGCGCACCTATAACGTTGGATTGTCCGCGTCGTGGGAGCTCGACCTGTTCGGCCGCGTGCAGAGCCTGAAGGAT
CAGGCGCTCGCGCAATACCTGTCGACCGCGTATGCGCGGCAGGCGTCGGAGATCTCGCTGGTGTCGCAGGTGGCGGATCA
ATACCTGACGCTGCTGTCGACCGACGACCTGCTGAAGGTCACGGAGGACACGCTGAAGACCGCGCAGGCATCGTACGACC
TGACCAAGCTGCAGTTCGACAACGGTACGGGCTCGGAGCTCGAGCTGCGTCAGGCGCAGACGGTCGTCGAGACGGCGCTC
GCGAACCAGCAGGCGCAGGCCCGTGCCCGCGCGCAGGCGCTGAACGCGCTGGTGCTGCTGATCGGCGAGCCGCTGCCGGA
CGACCTGCCGGCGGGCATGCCGCTCGATGCGCAGAACCTGCTGACGGACGTGCCCGCTGGCCTGCCGTCGGATCTGCTGA
CGCGTCGTCCTGACGTGATGCAGGCCGAGCAGACGCTGCTGGCCGCGAACGCGAACATCGGCGCGGCGCGCGCGGCGTTC
TTCCCGAAGATTTCGCTGACGGGCGCGTTCGGCACCGCGAGCCCGACGCTGGGCGGCCTGTTCAAGGCCGGCACGGCGGC
ATGGTCGTTCGCGCCGAGCATCGCACTGCCGATCTTCGAGGGCGGGCAGAACATCGCGAACCTCGATCTCGCGCACGTGC
AGAAGCGCATCGAGATCGCGAACTACGAGAAGGCGATCCAGTCGGCATTCCGCGAAGTGTCGGATGGCCTCGCCGCACGC
GGCACGTACGACCAGCAGATCGCGGCGCTCGAGCGCAACGAGCACGCGCAGCAACGTCGTTTCGACTTGTCGGACCTGCG
TTACAAGAACGGTGTCGACAGCTACCTGTCGGTGCTGACCGCGCAGACGGATCTGTACTCGGCGCAGCAGTCGCTGATCA
GCGCACGCCTGGCACGCTGGACGAACCTGGTCGACCTGTACCGCGCGCTCGGCGGCGGCTGGATCCAGCATGCCGGCGAA
GCGCCGCGCGCGCCGGATGCGCCGGTCGACTACGACAAGGCGGCGGCTCCTGCGCCCGCGTCGGCAACGGCGACGAACGG
GTAA

Upstream 100 bases:

>100_bases
GCGAGAAGGAAGACGTCGACGAGGCGCTGCGCCTGGCTCAGGAGCACTCGCACCACGAAGAGAAGCCGGGCAACGGCGAC
GAAGGCAACAAGGGACAGTG

Downstream 100 bases:

>100_bases
GCACCGAGAAGGGCGGGCGCGGGTCACCGCGCTTGCCCTTCGAACCGGCTGTATGCGGAAACGAGAACGCCACGGACGAA
AGTCCGTGGCGTTTTCTTTT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAATAIGWREFFVDPRLQRLIEIA
LKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNIQRLPAGVSTTRAPLISRTYNVGLSASWELDLFGRVQSLKD
QALAQYLSTAYARQASEISLVSQVADQYLTLLSTDDLLKVTEDTLKTAQASYDLTKLQFDNGTGSELELRQAQTVVETAL
ANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQTLLAANANIGAARAAF
FPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAAR
GTYDQQIAALERNEHAQQRRFDLSDLRYKNGVDSYLSVLTAQTDLYSAQQSLISARLARWTNLVDLYRALGGGWIQHAGE
APRAPDAPVDYDKAAAPAPASATATNG

Sequences:

>Translated_507_residues
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAATAIGWREFFVDPRLQRLIEIA
LKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNIQRLPAGVSTTRAPLISRTYNVGLSASWELDLFGRVQSLKD
QALAQYLSTAYARQASEISLVSQVADQYLTLLSTDDLLKVTEDTLKTAQASYDLTKLQFDNGTGSELELRQAQTVVETAL
ANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQTLLAANANIGAARAAF
FPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAAR
GTYDQQIAALERNEHAQQRRFDLSDLRYKNGVDSYLSVLTAQTDLYSAQQSLISARLARWTNLVDLYRALGGGWIQHAGE
APRAPDAPVDYDKAAAPAPASATATNG
>Mature_507_residues
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAATAIGWREFFVDPRLQRLIEIA
LKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNIQRLPAGVSTTRAPLISRTYNVGLSASWELDLFGRVQSLKD
QALAQYLSTAYARQASEISLVSQVADQYLTLLSTDDLLKVTEDTLKTAQASYDLTKLQFDNGTGSELELRQAQTVVETAL
ANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVMQAEQTLLAANANIGAARAAF
FPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAAR
GTYDQQIAALERNEHAQQRRFDLSDLRYKNGVDSYLSVLTAQTDLYSAQQSLISARLARWTNLVDLYRALGGGWIQHAGE
APRAPDAPVDYDKAAAPAPASATATNG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=474, Percent_Identity=41.3502109704641, Blast_Score=332, Evalue=5e-92,
Organism=Escherichia coli, GI2367348, Length=427, Percent_Identity=25.5269320843091, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082199, Length=318, Percent_Identity=23.2704402515723, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54161; Mature: 54161

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAAT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCEEE
AIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNI
EHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
QRLPAGVSTTRAPLISRTYNVGLSASWELDLFGRVQSLKDQALAQYLSTAYARQASEISL
EECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSQVADQYLTLLSTDDLLKVTEDTLKTAQASYDLTKLQFDNGTGSELELRQAQTVVETAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
ANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVM
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHH
QAEQTLLAANANIGAARAAFFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFE
HHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCEEEC
GGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHC
FDLSDLRYKNGVDSYLSVLTAQTDLYSAQQSLISARLARWTNLVDLYRALGGGWIQHAGE
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
APRAPDAPVDYDKAAAPAPASATATNG
CCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMQKHALTAIAVALFATGCTMAPHYKRPDAPVAQAYPAGGVYATQPGAAGARSANGQAAT
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCEEECCCCCCCCCCCCCCEEE
AIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNIEAARAQYQITRAGLFPTLDGTGTGNI
EHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCC
QRLPAGVSTTRAPLISRTYNVGLSASWELDLFGRVQSLKDQALAQYLSTAYARQASEISL
EECCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSQVADQYLTLLSTDDLLKVTEDTLKTAQASYDLTKLQFDNGTGSELELRQAQTVVETAL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
ANQQAQARARAQALNALVLLIGEPLPDDLPAGMPLDAQNLLTDVPAGLPSDLLTRRPDVM
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCHH
QAEQTLLAANANIGAARAAFFPKISLTGAFGTASPTLGGLFKAGTAAWSFAPSIALPIFE
HHHHHHHHCCCCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHHCCCCCCCEEEC
GGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQRR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHC
FDLSDLRYKNGVDSYLSVLTAQTDLYSAQQSLISARLARWTNLVDLYRALGGGWIQHAGE
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
APRAPDAPVDYDKAAAPAPASATATNG
CCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]