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The map label for this gene is flhA [H]

Identifier: 116690624

GI number: 116690624

Start: 2881750

End: 2882856

Strand: Reverse

Name: flhA [H]

Synonym: Bcen2424_2604

Alternate gene names: 116690624

Gene position: 2882856-2881750 (Counterclockwise)

Preceding gene: 116690625

Following gene: 116690623

Centisome position: 82.75

GC content: 65.22

Gene sequence:

>1107_bases
ATGAAAACGAAAGCCGCGATTGCATGGAAGGCGGGTGCGCCGCTGACGATCGAGGAAGTCGATCTCGAAGGGCCGCGCGC
CGGCGAAGTGCTGATCGAAGTGAAGGCGACGGGCATCTGCCACACCGACTACTACACGCTGTCGGGCGCCGATCCGGAAG
GGATCTTCCCGGCGATCCTCGGCCATGAAGGCGCGGGCGTGGTGGTCGACGTCGGCCCCGGCGTCGGCACGGTGCGCAAG
GGCGACCACGTGATTCCGCTCTACACGCCCGAATGCCGCGAGTGCAAGTTCTGCCTGTCGCGCAAGACCAACCTGTGCCA
GAAGATCCGCGCGACGCAGGGCAAGGGCCTGATGCCGGACGCGACGTCGCGCTTCTCGCTCGACGGCAAGCCGCTGTTCC
ACTACATGGGCACGTCGACGTTCTCGAACTACATCGTCGTGCCGGAGATCGCCGTCGCGAAGGTGCGCGAGGACGCGCCG
TTCGACAAGATCTGCTACATCGGCTGCGGCGTGACGACGGGCGTCGGCGCGGTCGTGTACTCGGCGAAGGTCGAGGCCGG
TGCGAACGTGGTCGTGTTCGGCCTCGGCGGGATCGGCCTGAACGTGATCCAGGGCGCGAAGATGGTCGGCGCGGACAAGA
TCATCGGCGTCGACATCAACCCGAAGCGCGTCGAGCTCGCGAAAAAGTTCGGGATGACGCACTTCATCAACCCGAACGAA
GTCGAGAACGTCGTCGACCACATCGTGCAGTTGACCGACGGCGGCGCCGACTATTCGTTCGAATGCGTCGGCAACGTGAA
GCTGATGCGCCAGGCGCTCGAATGCACGCACAAGGGCTGGGGCCAGTCGTTCATCATCGGCGTGGCGGCGGCGGGCGAGG
AGATCAGCACGCGTCCGTTCCAGCTGGTGACGGGCCGGGAGTGGAAGGGCTCGGCGTTCGGCGGCGCGCGCGGGCGCACC
GACGTGCCGAAGATCGTCGACTGGTACATGGAAGGCAAGATCAACATCGACGACCTGATCACGCACACGCTGCCGCTCGA
GCGGATCAACGAAGGCTTCGACCTGATGAAGGCCGGCGAGTCGATCCGCTCGGTCGTGCTGTACTGA

Upstream 100 bases:

>100_bases
CGCCGTGCCACGCGGCACGGCTTCGACCGCTTTGCACGAAACCCGGGCCGGCGCCGCACGCCGGCCCGGGCCGATCTCAC
CGACACACAGGAGTGACACG

Downstream 100 bases:

>100_bases
CCGGAGCGCACCGCCATGCTCGAACTCGTTTCCTCGCATGCGTGCCACGGCGGCGAGCAGCGTTTCTACCGTCACGATTC
GGCGGCCATCGGCCTGCCGA

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTVRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLERINEGFDLMKAGESIRSVVLY

Sequences:

>Translated_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTVRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLERINEGFDLMKAGESIRSVVLY
>Mature_368_residues
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAILGHEGAGVVVDVGPGVGTVRK
GDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAP
FDKICYIGCGVTTGVGAVVYSAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPFQLVTGREWKGSAFGGARGRT
DVPKIVDWYMEGKINIDDLITHTLPLERINEGFDLMKAGESIRSVVLY

Specific function: Essential for the oxidation of formaldehyde produced during methylotrophic growth [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=367, Percent_Identity=60.7629427792915, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI71565152, Length=374, Percent_Identity=47.3262032085562, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI34577061, Length=369, Percent_Identity=46.070460704607, Blast_Score=352, Evalue=4e-97,
Organism=Homo sapiens, GI4501933, Length=369, Percent_Identity=45.7994579945799, Blast_Score=347, Evalue=7e-96,
Organism=Homo sapiens, GI4501929, Length=369, Percent_Identity=45.5284552845528, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI262073058, Length=369, Percent_Identity=43.9024390243902, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI71743840, Length=369, Percent_Identity=43.9024390243902, Blast_Score=337, Evalue=1e-92,
Organism=Homo sapiens, GI156523966, Length=368, Percent_Identity=43.75, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI4501939, Length=361, Percent_Identity=43.7673130193906, Blast_Score=327, Evalue=1e-89,
Organism=Escherichia coli, GI1786552, Length=369, Percent_Identity=58.8075880758808, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI87082125, Length=379, Percent_Identity=26.9129287598945, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788073, Length=361, Percent_Identity=26.5927977839335, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790045, Length=376, Percent_Identity=27.1276595744681, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786825, Length=378, Percent_Identity=25.1322751322751, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1787863, Length=347, Percent_Identity=25.9365994236311, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081918, Length=342, Percent_Identity=28.6549707602339, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510992, Length=372, Percent_Identity=23.6559139784946, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=382, Percent_Identity=27.2251308900524, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788407, Length=229, Percent_Identity=27.9475982532751, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790718, Length=326, Percent_Identity=24.2331288343558, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786518, Length=367, Percent_Identity=24.5231607629428, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25146526, Length=373, Percent_Identity=54.6916890080429, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71997431, Length=375, Percent_Identity=54.4, Blast_Score=426, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17565904, Length=337, Percent_Identity=55.4896142433234, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17562584, Length=367, Percent_Identity=27.7929155313352, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17562876, Length=363, Percent_Identity=26.4462809917355, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562582, Length=330, Percent_Identity=25.7575757575758, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=373, Percent_Identity=26.0053619302949, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17562878, Length=360, Percent_Identity=24.7222222222222, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320033, Length=371, Percent_Identity=53.9083557951482, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323729, Length=364, Percent_Identity=27.7472527472527, Blast_Score=99, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323099, Length=371, Percent_Identity=28.3018867924528, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324486, Length=360, Percent_Identity=27.5, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6322619, Length=363, Percent_Identity=29.4765840220386, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319621, Length=372, Percent_Identity=27.1505376344086, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319955, Length=363, Percent_Identity=29.4765840220386, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323961, Length=364, Percent_Identity=25.8241758241758, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=366, Percent_Identity=25.6830601092896, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=258, Percent_Identity=28.6821705426357, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17737895, Length=368, Percent_Identity=60.054347826087, Blast_Score=476, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17137530, Length=341, Percent_Identity=27.2727272727273, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284 [H]

Molecular weight: Translated: 39535; Mature: 39535

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTVRKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCEEECCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTLPLERINEGFDLMKAGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCHHHHHHCH
SIRSVVLY
HHHHEECC
>Mature Secondary Structure
MKTKAAIAWKAGAPLTIEEVDLEGPRAGEVLIEVKATGICHTDYYTLSGADPEGIFPAIL
CCCCEEEEEECCCCEEEEEECCCCCCCCEEEEEEEECEEEECCEEEECCCCCCCCCHHHC
GHEGAGVVVDVGPGVGTVRKGDHVIPLYTPECRECKFCLSRKTNLCQKIRATQGKGLMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ATSRFSLDGKPLFHYMGTSTFSNYIVVPEIAVAKVREDAPFDKICYIGCGVTTGVGAVVY
CCCEEECCCCEEEEECCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCHHCCCCEEE
SAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVDINPKRVELAKKFGMTHFINPNE
EEEECCCCCEEEEEECCCCHHHHCCCEEECCCEEEEECCCCHHHHHHHHHCCEECCCCHH
VENVVDHIVQLTDGGADYSFECVGNVKLMRQALECTHKGWGQSFIIGVAAAGEEISTRPF
HHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCE
QLVTGREWKGSAFGGARGRTDVPKIVDWYMEGKINIDDLITHTLPLERINEGFDLMKAGE
EEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCHHHHCCCHHHHHHCH
SIRSVVLY
HHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7798140 [H]