Click here to switch to the map view.

The map label for this gene is braE [H]

Identifier: 116690516

GI number: 116690516

Start: 2765544

End: 2766713

Strand: Reverse

Name: braE [H]

Synonym: Bcen2424_2496

Alternate gene names: 116690516

Gene position: 2766713-2765544 (Counterclockwise)

Preceding gene: 116690517

Following gene: 116690515

Centisome position: 79.41

GC content: 65.04

Gene sequence:

>1170_bases
ATGACATCCATTCAACCGATCGAATCGTCGACGTCGCTGGTCGAAGAGCGCAATACCGCCAAGACCGTCGTCATCGGCAT
CCTGACCGCCGCGTTCGTGATCGCCGCGCCGATCATCATCGGCTCGGCCGGCGGCAACTACTGGGTCCGCGTGCTCGACT
TCGCGATGCTGTACGTGATGCTCGCGCTGGGCCTGAACGTGGTGGTCGGCTTCGCCGGCCTGCTCGACCTCGGCTACATC
GCGTTCTACGCGGTGGGCGCCTACACGGCGGCGCTCCTGAGCTCGCCGCACCTGACCTCGCAGTTCGAGTGGATCGCGGC
GCTCGCGCCGAACGGGCTGCACGTGCCGTTTCTGCTGATCGTGCCGATCGCGATGGCGCTGGCGGCGACCTTCGGGATCC
TGCTCGGCGCGCCGACGCTGCGCCTGCGCGGCGACTACCTGGCGATCGTCACGCTCGGCTTCGGTGAAATCGTCCGGATC
TTCATGAACAACCTCGACCGTCCGGTGAACATCACCAACGGCCCGAAGGGGATCACGGGCATCGATCCGGTGCACGTCGG
CGGCTTCAACCTGTCGCAGACGCATACGCTGTTCGGCTTCCAGCTGCCGTCGGTGTACATGTACTACTACCTGTTCGTGC
TCTGCTCGCTGCTGGTGATCTGGGTGTGTACGCGTCTGCAGCACTCGCGTATCGGCCGCGCGTGGGCTGCGATCCGCGAG
GACGAGATCGCCGCGAAGGCGATGGGCATCAACACCCGTAACGTGAAGCTGCTCGCGTTCGCGATGGGCGCGTCGTTCGG
TGGCCTGTCGGGCTCGATGTTCGGCGCGTTCCAGGGCTTCGTGTCGCCCGAGTCGTTCACGTTCTGGGAATCGATCGTCG
TGCTCGCCTGCGTGGTGCTCGGCGGCATGGGCCACATCCCGGGCGTGATCCTGGGCGCGGTGCTGCTCGCGATCTTCCCG
GAATTCCTGCGCTCGACGATGAGCCCGCTGCAGCACGCGCTGTTCGGTCATGACATCGTCGACACCGAAGTGATCCGTCA
GGCGCTGTACGGCCTCGCGATGGTGATCATCATGCTGTACCGCTCGGAAGGCCTGTGGCCCGCGCCGAAGCATGAGGACA
AGATCGCGAAACTGGCGAAGCGCAACAGCAAGAAGCCGGTGCGCGCTTAA

Upstream 100 bases:

>100_bases
TTCGCCTTCGTCGTGCTGATCATCGTGCTGGTGTTCCGTCCGTCGGGCCTGCTGGGCGAACGGGTCGCGGATCGCGCGTA
ACGGAAGGGGAGCAAACAAC

Downstream 100 bases:

>100_bases
CCTACGGACAAGGGGATACACACATGAGCGACAAGCAAATCCGACTGTCCGTCTCGGGCGTGAACAAGCGCTTCGGCGGG
CTGCAGGCGCTGTCCGACGT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIESSTSLVEERNTAKTVVIGILTAAFVIAAPIIIGSAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLLIVPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITGIDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAIFP
EFLRSTMSPLQHALFGHDIVDTEVIRQALYGLAMVIIMLYRSEGLWPAPKHEDKIAKLAKRNSKKPVRA

Sequences:

>Translated_389_residues
MTSIQPIESSTSLVEERNTAKTVVIGILTAAFVIAAPIIIGSAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLLIVPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNITNGPKGITGIDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAIFP
EFLRSTMSPLQHALFGHDIVDTEVIRQALYGLAMVIIMLYRSEGLWPAPKHEDKIAKLAKRNSKKPVRA
>Mature_388_residues
TSIQPIESSTSLVEERNTAKTVVIGILTAAFVIAAPIIIGSAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLLIVPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPVNITNGPKGITGIDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVLGGMGHIPGVILGAVLLAIFPE
FLRSTMSPLQHALFGHDIVDTEVIRQALYGLAMVIIMLYRSEGLWPAPKHEDKIAKLAKRNSKKPVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=348, Percent_Identity=42.2413793103448, Blast_Score=213, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 42019; Mature: 41888

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQPIESSTSLVEERNTAKTVVIGILTAAFVIAAPIIIGSAGGNYWVRVLDFAMLYVM
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHH
VPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
CCCCEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAIFPEFLRSTMSPLQHALFGHDIVDTEVIRQALYGLAMVIIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIAKLAKRNSKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSIQPIESSTSLVEERNTAKTVVIGILTAAFVIAAPIIIGSAGGNYWVRVLDFAMLYVM
CCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSQFEWIAALAPNGLHVPFLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHH
VPIAMALAATFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNITNGPKGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVHVGGFNLSQTHTLFGFQLPSVYMYYYLFVLCSLLVIWVCTRLQHSRIGRAWAAIRE
CCCCEECCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGSMFGAFQGFVSPESFTFWESIVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAIFPEFLRSTMSPLQHALFGHDIVDTEVIRQALYGLAMVIIMLY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDKIAKLAKRNSKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]